Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,508,433 Δ1 bp 6.3% intergenic (‑191/+28) fryB ← / ← glk putative enzyme IIB component of PTS/glucokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,508,4330A. 6.3% 232.7 / 9.6 79intergenic (‑191/+28)fryB/glkputative enzyme IIB component of PTS/glucokinase
Reads supporting (aligned to +/- strand):  ref base A (32/42);  new base . (3/2);  total (35/44)
Fisher's exact test for biased strand distribution p-value = 6.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01

AACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCGGAACCGAGAAGGCCCGGATTGTCATGGACGATGAGATACACCGGAATATCATGGACATATTCTTTAAAGCGCCCTTTA  >  NC_000913/2508285‑2508578
                                                                                                                                                    |                                                                                                                                                  
aaCGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGagag                                                                                                                                                   >  2:260987/1‑150 (MQ=255)
    tGCAAAACAAATCGCCGTTACCAGACAAATAAGGGATTTTCTGGAGAATTTATCCCGCTGTACGGCAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTTAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTAc                                                                                                                                               <  2:54909/150‑1 (MQ=255)
     gCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTAcc                                                                                                                                             >  2:596126/1‑150 (MQ=255)
      cAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCt                                                                                                                                            >  2:1297364/1‑150 (MQ=255)
      cAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCt                                                                                                                                            >  1:401456/1‑150 (MQ=255)
       aaaaCAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCGTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGTAGAGTTACCTc                                                                                                                                           >  1:374956/1‑150 (MQ=255)
         aaCAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTccc                                                                                                                                         <  2:278645/150‑1 (MQ=255)
         aaCAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTccc                                                                                                                                         <  1:623091/150‑1 (MQ=255)
         aaCAAATCGCCGTTACCAGACAAATAATGCATTTAATGGAGAAATAATCCAGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAAATGAATAAACTGTATGAATTAAAAGATTATCGGGAGAGTTACCTccc                                                                                                                                         >  2:724409/1‑150 (MQ=255)
            aaaTCGCCTTAACCAGACAAATAATTCATTTACTGGAGAATTAATCCCGCTGTACGGAATTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGat                                                                                                                                      <  2:596403/150‑1 (MQ=255)
            aaaTCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGGTAACTCTCCCGat                                                                                                                                      <  2:701120/150‑1 (MQ=255)
            aaaTCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGGTAACTCTCCCGat                                                                                                                                      <  2:779886/150‑1 (MQ=255)
               tCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGAtata                                                                                                                                   >  1:476183/1‑150 (MQ=255)
                 gCCGTTACCAGACAAATAAAGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATaaa                                                                                                                                 >  2:428045/1‑150 (MQ=255)
                       aCCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAaaggaag                                                                                                                           <  2:746914/150‑1 (MQ=255)
                       aCCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAaaggaag                                                                                                                           <  2:779623/150‑1 (MQ=255)
                         cAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAaggaagga                                                                                                                         >  1:659781/1‑150 (MQ=255)
                          aGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGAt                                                                                                                        >  2:714969/1‑150 (MQ=255)
                          aGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGAAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCTATATAAAAGGAAGGAt                                                                                                                        >  1:1263827/1‑150 (MQ=255)
                           gACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACATAGAAAAATTGAATAAACTGTTTGATTTAAAAGATTATCGGGAGAGTTACCTCCCGTTATAAAAGGAAGGAtt                                                                                                                       >  2:759581/1‑150 (MQ=255)
                           gACAAAAAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTAGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGAtt                                                                                                                       >  2:445482/1‑150 (MQ=255)
                              aaaTAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTAc                                                                                                                    <  1:830517/150‑1 (MQ=255)
                                ataatGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAg                                                                                                                  <  1:242504/150‑1 (MQ=255)
                                    tGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAAtg                                                                                                              <  1:38733/150‑1 (MQ=255)
                                    tGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAAtg                                                                                                              <  2:1289610/150‑1 (MQ=255)
                                    tGCATTTACTGGAGAATTAATCCAGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAAtg                                                                                                              <  1:31935/150‑1 (MQ=255)
                                       aTTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGTGACACAAAGAAAAATTGAATAAACTGTATGTTTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGa                                                                                                           <  1:1112492/150‑1 (MQ=255)
                                       aTTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGa                                                                                                           <  2:759227/150‑1 (MQ=255)
                                       aTTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGa                                                                                                           <  2:1100690/150‑1 (MQ=255)
                                       aTTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGGGAGGGGACACAAAGAAAAATTGAATACACTCTATGATTTAAAAGATTACCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGa                                                                                                           <  1:1233902/150‑1 (MQ=255)
                                        tttACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGAc                                                                                                          <  1:515687/150‑1 (MQ=255)
                                         atACTGGAGAATTAATCCCGCTTTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGAcc                                                                                                         <  2:517487/149‑1 (MQ=255)
                                          tACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAATCTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCt                                                                                                        >  1:94193/1‑150 (MQ=255)
                                          tACTGGAGAATTAATCCCGCTGTACCGAAGTGTTACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATAGAATATACTGTATGCTTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGGGACCt                                                                                                        <  2:122363/150‑1 (MQ=255)
                                            cTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTAAAGAATGTGACCTaa                                                                                                      >  2:675406/1‑150 (MQ=255)
                                               gagaATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGt                                                                                                   <  1:1108715/150‑1 (MQ=255)
                                                 taaTTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCt                                                                                                 <  2:829894/149‑1 (MQ=255)
                                                     tAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCg                                                                                             <  1:834412/150‑1 (MQ=255)
                                                       aTCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTa                                                                                           <  1:1073538/150‑1 (MQ=255)
                                                       aTCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTa                                                                                           >  2:198396/1‑150 (MQ=255)
                                                       aTCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTATGGCGTa                                                                                           >  1:408187/1‑150 (MQ=255)
                                                              tGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGc                                                                                    <  2:1060202/150‑1 (MQ=255)
                                                                 aCGGAAGTGTGACGCAGGTCTACCTTGTTAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCGGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCAcc                                                                                 <  2:24939/150‑1 (MQ=255)
                                                                  cGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAATCTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCAACg                                                                                >  1:197602/1‑150 (MQ=255)
                                                                  cGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCg                                                                                >  1:1077783/1‑150 (MQ=255)
                                                                    gAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCGGa                                                                              >  2:498147/1‑150 (MQ=255)
                                                                        tgtgACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAATGTCTGGCGTAAATGTGCACCGGAACCg                                                                          >  1:276659/1‑150 (MQ=255)
                                                                        tgtgACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCGGAACCg                                                                          <  2:1057512/150‑1 (MQ=255)
                                                                          tgACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCGGAACCgag                                                                        <  1:262204/150‑1 (MQ=255)
                                                                            actcAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATATAAAAGATTATCGGCAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCGGAACCGAGaa                                                                      <  2:506787/147‑1 (MQ=255)
                                                                             cGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCGGAACCGAGAAg                                                                     <  2:78271/150‑1 (MQ=255)
                                                                             cGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACATCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCGGAACCGAGAAg                                                                     >  1:1140832/1‑150 (MQ=255)
                                                                              gCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCGGAACCGAGAAgg                                                                    >  2:473493/1‑150 (MQ=255)
                                                                                 ggTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCGGAACCGAGAAGGccc                                                                 >  1:1075377/1‑150 (MQ=255)
                                                                                  gTCGACATTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAATGGAAGGATTTACAGAATGTGACCTAAGGTGTGGCGTAAATGTGCACCGGAACCGAGAAGGCCCg                                                                >  2:890865/1‑150 (MQ=255)
                                                                                     gACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCGGAACCGAGAAGGCCCGGAt                                                             >  2:682405/1‑150 (MQ=255)
                                                                                     gACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCGGAACCGAGAAGGCCCGGAt                                                             <  1:452603/150‑1 (MQ=255)
                                                                                             gAGGTGACAGAAAGAAAAATTGAAATAACTGTTTGATATAAAAGATTATCGGGAGAGGTACCTCCCGATATTAAATGAATGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCGGAACCGAGAAGGCCCGGATTGTCATgg                                                     <  2:827249/150‑1 (MQ=255)
                                                                                               ggggACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCGGAACCGAGAAGGCCCGGATTGTCATGGAc                                                   <  1:351608/150‑1 (MQ=255)
                                                                                                    cacaAAGATATATTGAATAAACTGTATGATTTAAAAGATTAACGGGAGAGGTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGAAAATGTGCACCGGAACCGAGAAGGCCCGGATTGTCATGGTCGATga                                              <  2:157143/150‑1 (MQ=255)
                                                                                                    cacaAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCGGAACCGAGAAGGCCCGGATTGTCATGGACGATga                                              <  2:620318/150‑1 (MQ=255)
                                                                                                    cacaAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCGGAACCGAGAAGGCCCGGATTGTCATGGACGATga                                              >  1:620318/1‑150 (MQ=255)
                                                                                                      caAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATTTAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCGGAACCGAGAAGGCCCGGATTGTCATGGACGATgaga                                            >  2:542474/1‑150 (MQ=255)
                                                                                                      caAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCACGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCGGAACCGAGAAGGCCCGGATTGTCATTGACGATgaga                                            >  2:957733/1‑150 (MQ=255)
                                                                                                       aaaGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGTAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCGGAACCGAGAAGGCCCGGATTGTCATGGACGATGAGAt                                           <  2:949381/150‑1 (MQ=255)
                                                                                                              aaTTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCGGAACCGAGAAGGCCCGGATTGTCATGGACGATGAGATACACCgg                                    >  1:872131/1‑150 (MQ=255)
                                                                                                              aaTTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCGGAACCGAGAAGGCCCGGATTGTCATGGACGATGAGATACACCgg                                    >  1:370402/1‑150 (MQ=255)
                                                                                                              aaTTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCGGAACCGAGAAGGCCCGGATTGTCATGGACGATGAGATACACCgg                                    >  1:375925/1‑150 (MQ=255)
                                                                                                                           gTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCGGAACCGAGAAGGCCCGGATTGTCATGGACGATGAGATACACCGGAATATCATGGACa                       <  2:683442/150‑1 (MQ=255)
                                                                                                                              tGATTTAAAATATTATCGGGAGATTTACCTCCAGATATAAAAGGAATGATTTACAGAATGTGACCTAAGTTCTGGCGTAAATGTGCACCGGAACCGAGAAGGCCCGGATTTTCATGGACGATGAGATACACCGGAATATCATGGACatat                    <  1:360314/150‑1 (MQ=255)
                                                                                                                                   tAAAAGATTATCGGGATAGTTACCTCCAGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCGGAACCGAGAAGGCCCGGATTGTCATGGACGATGAGATACACCGGAATATCATGGACATATTCttt               <  2:1263827/150‑1 (MQ=255)
                                                                                                                                     cttttatATCGGGAGGTAACTCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCGGAACCGAGAAGGCCCGGATTGTCATGGACGATGAGATACACCGGAATATCATGGACATATTCTTTaa             >  1:357774/7‑150 (MQ=255)
                                                                                                                                     aaaGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAATTGTGCACCGGAACCGAGAAGGCCCGGATTGTCATGGACGATGAGATACACCGGAATATCATGGACATATTCTTTaa             >  2:1015425/1‑150 (MQ=255)
                                                                                                                                       aGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCGGAACCGAGAAGGCCCGGATTGTCATGGACGATGAGATACACCGGAATATCATGGACATATTCTTTAAAg           <  2:374956/150‑1 (MQ=255)
                                                                                                                                        caTTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCGGAACCGAGAAGGCCCGGATTGTCATGGACGATGAGATACACCGGAATATCATGGACATATTCTTTAAAgc          <  2:186132/149‑1 (MQ=255)
                                                                                                                                         tatATCGGGAGGTAACTCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCGGAACCGAGAAGGCCCGGATTGTCATGGACGATGAGATACACCGGAATATCATGGACATATTCTTTAAAgcg         >  2:884943/3‑150 (MQ=255)
                                                                                                                                           tATCGGGAGGTAACTCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCGGAACCGAGAAGGCCCGGATTGTCATGGACGATGAGATACACCGGAATATCATGGACATATTCTTTAAAGCGcc       >  1:975534/1‑150 (MQ=255)
                                                                                                                                            aTCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCGGAACCGAGAAGGCCCGGATTGTCATGGACGATGAGATACACCGGAATATCATGGACATATTCTTTAAAGCGccc      <  2:415584/150‑1 (MQ=255)
                                                                                                                                            aTCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCGGAACCGAGAAGGCCCGGATTGGCATGGACGATGAGATACACCGGAATATCATGGACATATTCTTTAAAGCGccc      <  2:659781/150‑1 (MQ=255)
                                                                                                                                                ggAGAGTTACCTCCAGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCGGCACCGAGAAGGCCCGGATTGTCATGGACGATGAGATACACCGGAATATCATGGACATATTCTTTAAAGCGCCCTTTa  <  2:476183/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGCACCGGAACCGAGAAGGCCCGGATTGTCATGGACGATGAGATACACCGGAATATCATGGACATATTCTTTAAAGCGCCCTTTA  >  NC_000913/2508285‑2508578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: