Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,757,939 Δ1 bp 6.2% intergenic (‑112/+78) yiaY ← / ← selB L‑threonine dehydrogenase/selenocysteinyl‑tRNA‑specific translation factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,757,9390A. 6.2% 241.5 / 9.5 81intergenic (‑112/+78)yiaY/selBL‑threonine dehydrogenase/selenocysteinyl‑tRNA‑specific translation factor
Reads supporting (aligned to +/- strand):  ref base A (39/37);  new base . (2/3);  total (41/40)
Fisher's exact test for biased strand distribution p-value = 6.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.34e-01

ATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTAAAGCCAATG  >  NC_000913/3757792‑3758086
                                                                                                                                                   |                                                                                                                                                    
aTTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTAtt                                                                                                                                                    <  2:660075/150‑1 (MQ=255)
   cacaGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCAt                                                                                                                                                 >  1:399517/1‑150 (MQ=255)
     caGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTTAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTa                                                                                                                                               <  1:977704/150‑1 (MQ=255)
     caGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTa                                                                                                                                               >  1:398215/1‑150 (MQ=255)
        aaGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAAc                                                                                                                                           <  2:983279/150‑1 (MQ=255)
        aaGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAAc                                                                                                                                           <  2:328289/150‑1 (MQ=255)
        aaGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAAc                                                                                                                                           <  1:1250870/150‑1 (MQ=255)
         aGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCTTTAAACa                                                                                                                                          >  1:549184/1‑150 (MQ=255)
         aGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACa                                                                                                                                          >  2:395168/1‑150 (MQ=255)
         aGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACa                                                                                                                                          <  1:1190261/150‑1 (MQ=255)
           gAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGAACATTGTATTCATTAAACAAt                                                                                                                                        >  1:646939/1‑150 (MQ=255)
             aTAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAAtgt                                                                                                                                      <  2:151911/150‑1 (MQ=255)
               aaaGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCATCTTACTGAGGATTATCATTCGAAAATATGATGATTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTTTAATGAATACAATGTGc                                                                                                                                    <  2:733045/150‑1 (MQ=255)
               aaaGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGc                                                                                                                                    <  2:151604/150‑1 (MQ=255)
                  gAACGTTTAATCTGCCATAACACACTACTGAAATTTTAAAAAAGGTCTGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTTTAATGAATACAATGTGCttt                                                                                                                                 <  1:680717/150‑1 (MQ=255)
                     cGTTGAAGCTGCCATAACACCCTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATACATTAAACAATGTGCtttttt                                                                                                                              >  1:1007060/1‑150 (MQ=255)
                     cGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCtttttt                                                                                                                              <  1:754974/150‑1 (MQ=255)
                      gTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCttttttt                                                                                                                             >  1:1161905/1‑150 (MQ=255)
                       ttGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTTTCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTa                                                                                                                            <  1:967365/150‑1 (MQ=255)
                        tgtaGCTGCCATTACACACTTCTTTAAGTGTAAAAAAGGTCGGGCAGCTTACTGAGGATTTTCATTCGAAAATAGGATCAGTATTATTGATTATCATTATTAATCTAATAAAAAGCACATTGTTTAATGAATACAATGTGCtttttatta                                                                                                                           <  1:647598/147‑5 (MQ=25)
                        tGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTaa                                                                                                                           <  2:251719/150‑1 (MQ=255)
                           aGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAAtt                                                                                                                        >  2:901427/1‑150 (MQ=255)
                           aGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAAtt                                                                                                                        >  1:679013/1‑150 (MQ=255)
                            gCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTc                                                                                                                       >  2:510515/1‑150 (MQ=255)
                            gCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCAAATTTTATTCATTAAAAAATGTGCTTTTTTTAAAtta                                                                                                                       >  2:726017/1‑149 (MQ=255)
                              tGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCAt                                                                                                                     >  2:710601/1‑150 (MQ=255)
                               gCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATa                                                                                                                    <  2:795213/150‑1 (MQ=255)
                               gCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATa                                                                                                                    <  1:734579/150‑1 (MQ=255)
                               gCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGAACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATa                                                                                                                    >  2:515431/1‑150 (MQ=255)
                                 cATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATaat                                                                                                                  >  1:452007/1‑149 (MQ=255)
                                          cTCCTTAAAGTTTATAAAAGTGCGTGCAGCTTATAGAGGATTTTCATTCTAAAATATGATCAGTATTATTGATTATCAATATTAATCTAATAAAAATCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCTTATGAAtttaaaa                                                                                                         <  1:274328/150‑5 (MQ=255)
                                                     gtAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAatttattt                                                                                              >  1:771325/1‑150 (MQ=255)
                                                      tAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTAtttt                                                                                             <  2:1083485/150‑1 (MQ=255)
                                                        aaaaaGGGCGTGCAGCTTAATGATGTTTTTCATTAGAAAATATGATCAGTATTATTGATTATAAAAATTAATCTAATAAAAAGCACATTGTATTCATTATACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTaa                                                                                           <  1:641941/150‑1 (MQ=255)
                                                             gggcgggcAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACtt                                                                                      >  1:8834/1‑150 (MQ=255)
                                                                 gggcAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACttaatt                                                                                  <  1:264602/150‑1 (MQ=255)
                                                                      tattACTGATGATTTTCATTCGAAAATATGATGAGTATTATTTATTATCAAAATTAATCTAATAAAAAGCAAATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCa                                                                             <  1:923304/148‑1 (MQ=255)
                                                                      gCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCa                                                                             <  2:369512/150‑1 (MQ=255)
                                                                        ttACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCAtt                                                                           >  2:1063109/1‑150 (MQ=255)
                                                                         tACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCAttt                                                                          >  2:34257/1‑150 (MQ=255)
                                                                               ggATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTAt                                                                    <  1:1184207/150‑1 (MQ=255)
                                                                                 aTTTTCATTAGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTAttt                                                                  <  1:879688/150‑1 (MQ=255)
                                                                                     tCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTcc                                                              <  2:753052/150‑1 (MQ=255)
                                                                                      cATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCg                                                             >  2:146093/1‑150 (MQ=255)
                                                                                      cATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCg                                                             >  1:1072733/1‑150 (MQ=255)
                                                                                             aaaTATGATCATTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAaataa                                                      <  1:109748/150‑1 (MQ=255)
                                                                                             aaaTATGATCAGAATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTCTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCATGAaataa                                                      >  2:830507/1‑150 (MQ=255)
                                                                                                tatGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAaataataa                                                   <  1:1087909/150‑1 (MQ=255)
                                                                                                  tGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATg                                                 >  1:393085/1‑150 (MQ=255)
                                                                                                  tGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATg                                                 <  2:466741/150‑1 (MQ=255)
                                                                                                   taTCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGc                                                <  1:390620/149‑1 (MQ=255)
                                                                                                    aTCAGTATTATTGATTATCAATATTAATCTAATAAAAAGCACAATGTATTCATTTAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTATAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCg                                               <  2:846099/150‑1 (MQ=255)
                                                                                                      cAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCGTTATTTTTCCGGAAATAATAATGCGTc                                             >  2:489433/1‑150 (MQ=255)
                                                                                                       aGTATTATTGATTATAAAAATTAATCTAATAAAAAGGACATTGTATTCATTAATCAATTTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCTGAAATAATTTTGCGTcg                                            <  1:519532/150‑1 (MQ=255)
                                                                                                           ttattGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGTAAATAATAATGCGTCGCGCa                                        >  1:558719/1‑150 (MQ=255)
                                                                                                           ttattGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCa                                        <  2:831134/150‑1 (MQ=255)
                                                                                                              ttGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATa                                     <  2:1077941/150‑1 (MQ=255)
                                                                                                               tGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATaa                                    >  2:653929/1‑150 (MQ=255)
                                                                                                                     tCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATc                              <  2:716077/150‑1 (MQ=255)
                                                                                                                      cAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCa                             <  2:372435/150‑1 (MQ=255)
                                                                                                                       aaaaTTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCAt                            >  1:1214539/1‑150 (MQ=255)
                                                                                                                        aaaTTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGAGTCGCGCAATAAATGATCAtt                           >  2:1268614/1‑150 (MQ=255)
                                                                                                                            tAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCa                       <  1:1013261/150‑1 (MQ=255)
                                                                                                                             aaTCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCac                      >  2:1300355/1‑150 (MQ=255)
                                                                                                                              aTCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCacg                     <  1:1063109/150‑1 (MQ=255)
                                                                                                                              aTCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCacg                     >  1:700269/1‑150 (MQ=255)
                                                                                                                                cTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGAGTCGCGCAATAAATGATCATTTcaaagaa                   >  1:212528/1‑144 (MQ=255)
                                                                                                                                  aataaAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCacgacga                 >  2:599520/1‑150 (MQ=255)
                                                                                                                                  aataaAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCacgacga                 >  2:605027/1‑150 (MQ=255)
                                                                                                                                      aaaaGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGAcgcg             <  2:8834/150‑1 (MQ=255)
                                                                                                                                        aaGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCTCAATAAATGATCATTTCCACGACGACGCTTa           >  2:185844/1‑150 (MQ=255)
                                                                                                                                        aaGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTa           <  2:1020877/150‑1 (MQ=255)
                                                                                                                                        aaGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTa           >  2:1062619/1‑150 (MQ=255)
                                                                                                                                         aGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCAACGACGACGCGTaa          >  1:507201/1‑150 (MQ=255)
                                                                                                                                         aGCACATTGTTTAATGAATACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTaa          >  2:521762/1‑150 (MQ=255)
                                                                                                                                          gCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTaaa         >  2:178689/1‑150 (MQ=255)
                                                                                                                                          gCACATTGTTTAATGAATACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTaaa         >  1:484577/1‑150 (MQ=255)
                                                                                                                                          ccaaaTTGTATTAATTAAACAATGTGCTTTTTTTAAATTCATAATAATTTTGTATTAATTTATTTTAAAACATAATTAATCATTTCCTTATTTTTCCGGTAATAATAATGCGTCTCGCAATAAATGATCATTTCCACGACGACGCGTaaa         <  1:832277/146‑1 (MQ=255)
                                                                                                                                             caTTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCTTAAAGcc      >  2:159804/1‑150 (MQ=255)
                                                                                                                                             caTTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTACTTATTTTTCCTGAAATAATAATTCTTCTCGAAATAAATTATCATTTCCAAGACGACTAGTAAAgaa      >  1:113939/1‑148 (MQ=255)
                                                                                                                                                 gTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATTCGTCGCGCAATAAATTATCATTTCCACGACGACGCGTAAAGCCAATg  >  2:215113/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ATTCACAGAAGGAATAAAGAACGTTGAAGCTGCCATAACACACTCCTGAAAGTGTAAAAAAGGGCGGGCAGCTTACTGAGGATTTTCATTCGAAAATATGATCAGTATTATTGATTATCAAAATTAATCTAATAAAAAGCACATTGTATTCATTAAACAATGTGCTTTTTTTAAATTCATAAGAATTTTGTATTAATTTATTTTAAAACTTAATTAATCATTTCCTTATTTTTCCGGAAATAATAATGCGTCGCGCAATAAATGATCATTTCCACGACGACGCGTAAAGCCAATG  >  NC_000913/3757792‑3758086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: