Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,390,016:1 +C 5.1% intergenic (+52/+32) yfbP → / ← nuoN TPR‑like repeats‑containing protein/NADH:ubiquinone oxidoreductase, membrane subunit N

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,390,0161.C 5.1% 321.8 / 12.5 79intergenic (+52/+32)yfbP/nuoNTPR‑like repeats‑containing protein/NADH:ubiquinone oxidoreductase, membrane subunit N
Reads supporting (aligned to +/- strand):  ref base . (37/38);  new base C (2/2);  total (39/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.74e-01

GGAACGCTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTGTACTGCC  >  NC_000913/2389871‑2390163
                                                                                                                                                  |                                                                                                                                                    
ggAACGCTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTcgg                                                                                                                                                  <  1:896863/150‑1 (MQ=255)
ggAACGCTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTACACTTAACGCTCTGCGTATATCTAATATACCTTCTTTTTCAGAAAAACCGCCGAGCAAGTcgg                                                                                                                                                  >  2:195648/1‑150 (MQ=255)
 gAACGCTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTcggc                                                                                                                                                 <  1:375057/150‑1 (MQ=255)
    cGCTATGATTTTAAAATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGACATGCTcggcggt                                                                                                                                              <  2:1177469/150‑2 (MQ=255)
    agCTATTATTTTAATCTCGATGAATGGCGGGCAAAATTCAGGCAGGTATTTTTTGAGTAGGTTGGCGATAGTCCTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGTGCATGTCggcggc                                                                                                                                             <  2:801153/149‑1 (MQ=255)
      cTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCtt                                                                                                                                           <  2:1198148/150‑1 (MQ=255)
      cTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCtt                                                                                                                                           <  1:1314909/150‑1 (MQ=255)
        aTGATTTTAATATAGATGAATGGCGTGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGGCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTc                                                                                                                                         <  1:109458/150‑1 (MQ=255)
         tGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCGTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCt                                                                                                                                        >  1:806522/1‑150 (MQ=255)
         tGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCt                                                                                                                                        >  2:484953/1‑150 (MQ=255)
           aTTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAGCCGCCGACATGCTCGGCGGtttttctga                                                                                                                                     <  1:860736/150‑6 (MQ=38)
                     cGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAACACCGCCGCGCATGTCGGCGGCTTTCTGACTTACAAAGt                                                                                                                            >  1:501202/1‑150 (MQ=255)
                        tGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAAc                                                                                                                         <  2:686724/150‑1 (MQ=255)
                         gAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACa                                                                                                                        <  2:955795/150‑1 (MQ=255)
                              gctggCATAATTGACGCTGTTATGTTTAGAGTATTTTTGCGATAGTCGTACACAGTTCACTTAATGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGAATGTGGGCGGCTTTCTGACTTACAAAGTAACAGATTa                                                                                                                   <  2:574352/147‑1 (MQ=255)
                               cGGGCAAAATTCAGGCAGTTATGTGTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCAAGTCGGCGGCTTTCTGACTTACAAAGTAACAGATGAc                                                                                                                  >  2:207044/1‑150 (MQ=255)
                                 ggCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAACCCGCCGAGCATGGCGGAGGCTTTCTGACTTACAAAGTACCAGATTACAt                                                                                                                >  2:490078/1‑150 (MQ=255)
                                 ggCAAAATTCAGGCAGTTAGGTTTAGAGTATTTTGGCGATAGTCGTGCACAGGTCACTTAACGCTCTGCGTCTATCTAGTAGCCCTTCTTTTTCAGAAAAACCGCGGGGCTTGTCGGGGGCTTTCTGGCTGAGAAAGGAACGGGTTAcct                                                                                                                >  2:851578/1‑148 (MQ=255)
                                  gCAATATTAAGGCAGTGATGTTTAGAGTATTTTGGAGATAGTCGTACACATTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATc                                                                                                               <  2:1170106/150‑1 (MQ=255)
                                  gCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATc                                                                                                               <  2:1051371/150‑1 (MQ=255)
                                         tCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCa                                                                                                        <  2:856226/150‑1 (MQ=255)
                                         tCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCa                                                                                                        <  2:194909/150‑1 (MQ=255)
                                             gCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTTACAGATTACATCAGCGGCATTGc                                                                                                    <  1:195887/150‑1 (MQ=255)
                                             gCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCAGAGCATATCGGCGGCTTTCTGACATAAAAAGTAACAGATTACATCAGCGGCAATGc                                                                                                    >  2:1189227/1‑150 (MQ=255)
                                             gCAGCTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGc                                                                                                    <  1:451158/150‑1 (MQ=255)
                                               aGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCa                                                                                                  >  1:772052/1‑150 (MQ=255)
                                                gTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCaa                                                                                                 >  2:775656/1‑150 (MQ=255)
                                                   aTGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGGCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACg                                                                                              <  1:1160301/150‑1 (MQ=255)
                                                    tGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGc                                                                                             >  1:1161203/1‑150 (MQ=255)
                                                        tAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACaa                                                                                         >  2:1239757/1‑150 (MQ=255)
                                                          gagTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATg                                                                                       <  1:661004/150‑1 (MQ=255)
                                                           agTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGc                                                                                      <  1:261585/150‑1 (MQ=255)
                                                              aTTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTaa                                                                                   >  2:554907/1‑150 (MQ=255)
                                                              aTTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATATACTATCCATTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGAACAATGCTaa                                                                                   >  1:1245596/1‑150 (MQ=255)
                                                               ttttGGCGATAGTCGTACACAGTTCACTTAAGGCGCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGGCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAAt                                                                                  <  2:483583/150‑1 (MQ=255)
                                                                tttGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATc                                                                                 >  2:286577/1‑150 (MQ=255)
                                                                tttGGCGATAGTCGTACACAGTTCACATAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCTCCGAGCATGTCGGCGGCATTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATc                                                                                 <  1:286375/150‑1 (MQ=255)
                                                                 ttGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCa                                                                                >  1:1026407/1‑150 (MQ=255)
                                                                  tGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCAGGGCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCCGCGGCATTGCCAAACGCACAATGCTAATCAg                                                                               >  2:798596/1‑150 (MQ=255)
                                                                    gCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTAAATCAGCGGCATTGCCAAACGCACGATGCTAATCAGCg                                                                             >  1:194166/1‑150 (MQ=255)
                                                                      gATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGt                                                                           <  2:1267351/150‑1 (MQ=255)
                                                                         aGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTtgt                                                                        >  1:1189901/1‑150 (MQ=255)
                                                                           tCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTgg                                                                      <  2:1037936/150‑1 (MQ=255)
                                                                                  caGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATacc                                                               >  1:524793/1‑149 (MQ=255)
                                                                                  caGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCAAGTCGGCGGCATTCTGACTTACAAAGTAACAGATTACATCAGCGTCATTGCCAAACGCACAATACTAATCAGCGGTTGTGGCCAAaca                                                               >  2:62198/1‑150 (MQ=255)
                                                                                   aGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATacac                                                              <  1:361766/150‑1 (MQ=255)
                                                                                   aGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATacac                                                              >  1:414372/1‑150 (MQ=255)
                                                                                      tCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCa                                                           <  2:1012920/150‑1 (MQ=255)
                                                                                       cACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAAAAGATTACATCAGCGGCATTGCCAAACGCACAATTCTAATCAGCGGTTGTGGCCATACACCCAg                                                          >  1:753961/1‑150 (MQ=255)
                                                                                        agttAACTCTCTGGGTGTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAAAAGATTACATCAGCGGCATTGCCAAACGCACAATTCTAATCAGCGGTTGTGGCCATACACCCAgg                                                         <  2:543046/148‑2 (MQ=255)
                                                                                           tAACGCTCTGCGTCTATCTACTATCCCTTCTATTTCAGAAAAACCGCCGAGCATGGCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCAcc                                                      <  2:124421/150‑1 (MQ=255)
                                                                                              cGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCTGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGt                                                   <  1:712664/150‑1 (MQ=255)
                                                                                                        ctatctaCTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACATAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATCCACCCAGCACCAGTACCCACAGTg                                         >  2:550200/1‑150 (MQ=255)
                                                                                                        ctatctaCTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTg                                         <  2:438653/150‑1 (MQ=255)
                                                                                                         tatctaCTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACAACAGCGGCATTGCCAAACGCACAATGCTAATCAGCGTTTGTGGCAATACACCCAGCACCAGTACCAACAGTGc                                        >  1:619253/1‑150 (MQ=255)
                                                                                                              actaTCCCTTCTTTTTCAGAACAACCGCCGAGCATGGCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAgaga                                   <  2:459139/150‑1 (MQ=255)
                                                                                                              actaTCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCTGCTTTCTGACTTACAAAGTAACAGATTACATCAGAGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGAGGCCATACACCCAGAACCAGTACAAACAGAGCAgaga                                   >  1:198386/1‑150 (MQ=255)
                                                                                                                 aTCCCTTCTTTTTCAGAAAAACCGCCGCGCATGGCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCa                                <  1:697583/150‑1 (MQ=255)
                                                                                                                 aTCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCa                                >  2:456688/1‑150 (MQ=255)
                                                                                                                  tCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAg                               >  1:26524/1‑150 (MQ=255)
                                                                                                                   cccTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGc                              >  1:579153/1‑150 (MQ=255)
                                                                                                                    ccTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGTTTACATCAGCGGCATTGCCAATCGCACAATGCTAATCAGCGGTTGTGGCCATACAGCCAGCACCAGTACCAACAGTGCAGAGATCAGca                             >  1:63911/1‑150 (MQ=255)
                                                                                                                    ccTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCTTTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGca                             <  2:392164/150‑1 (MQ=255)
                                                                                                                    ccTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGca                             <  2:1210263/150‑1 (MQ=255)
                                                                                                                      ttcttTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGcaca                           <  1:574806/150‑1 (MQ=255)
                                                                                                                            agtcagaaagCCGCCGACATGCTCGGCGGTTTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGAt                      >  1:677833/7‑150 (MQ=38)
                                                                                                                            agtcagaaagCCGCCGACATGCTCGGCGGTTTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGAt                      >  1:783312/7‑150 (MQ=38)
                                                                                                                             tCAGAAAAACGGCCGAGGATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCAGAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATAc                    <  1:80365/150‑1 (MQ=255)
                                                                                                                             tCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGTCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATAc                    <  1:83390/150‑1 (MQ=255)
                                                                                                                              cAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATAcc                   >  1:1099758/1‑150 (MQ=255)
                                                                                                                               aGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATAccg                  <  2:1161203/150‑1 (MQ=255)
                                                                                                                               aGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATAccg                  >  1:328563/1‑150 (MQ=255)
                                                                                                                                gAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACAGCAGCGGCATTGCCACACGCACATTGCTAATCCGCGGTTTTGGCCATACACCCCGCACCAGTACCACCAGTGCAGAGATCAGAACAACGATTccgc                 >  1:865171/1‑150 (MQ=255)
                                                                                                                                  aaaaCCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATAccgccc               >  1:863141/1‑150 (MQ=255)
                                                                                                                                   aaaCCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATAccgcccg              >  1:896718/1‑150 (MQ=255)
                                                                                                                                        gccgAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACCACGATACCGCCCGCGCTg         >  2:747932/1‑150 (MQ=255)
                                                                                                                                        gccgAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTg         <  1:1132746/150‑1 (MQ=255)
                                                                                                                                              cATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTGTACTGc   >  1:818816/1‑150 (MQ=255)
                                                                                                                                               aTGTCGGCGGCTTTCTGACTTGCAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTACTCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTGTACTGcc  <  1:125086/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GGAACGCTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTGTACTGCC  >  NC_000913/2389871‑2390163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: