Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,991,805:1 +T 5.3% intergenic (+46/‑289) ygeG → / → ygeH SycD‑like chaperone family TPR‑repeat‑containing protein/putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,991,8051.T 5.3% 373.8 / 15.3 94intergenic (+46/‑289)ygeG/ygeHSycD‑like chaperone family TPR‑repeat‑containing protein/putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base . (42/47);  new base T (3/2);  total (45/49)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.78e-01

AATGTTTCTATCACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATAAGGCTATTTCAGATTACATCACTGAATTCC  >  NC_000913/2991656‑2991954
                                                                                                                                                      |                                                                                                                                                      
aatgTTTCTATCACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAc                                                                                                                                                         >  2:529097/1‑150 (MQ=255)
 atgTTTCTATAACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAc‑‑c                                                                                                                                                      <  1:841358/150‑1 (MQ=255)
  tgTTTCTATCACTATATTAACAATTGTGAGGAAGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑Ca                                                                                                                                                     <  2:812901/150‑1 (MQ=255)
       cTATCACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACtata                                                                                                                                                <  2:1019463/150‑1 (MQ=255)
        tATCACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATaa                                                                                                                                               <  1:119805/150‑1 (MQ=255)
        tATCACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATaa                                                                                                                                               <  1:1264554/150‑1 (MQ=255)
          tCACATTATTAACATTTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATTGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAAcc                                                                                                                                             <  1:893171/150‑1 (MQ=255)
          tCACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTTAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAAcc                                                                                                                                             <  2:637830/150‑1 (MQ=255)
          tCACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTG‑‑GTTAACTGCACTATAAcc                                                                                                                                             <  2:944324/150‑1 (MQ=255)
             aattattAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTG‑‑GTTAACTGCACTATAACCAGc                                                                                                                                          <  1:1238952/149‑1 (MQ=255)
                 attaACAATTGTGAGGATGATAGTGTTAAGAGTAAAGATCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACct                                                                                                                                      >  2:1258496/1‑150 (MQ=255)
                   taACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACctct                                                                                                                                    <  1:825424/150‑1 (MQ=255)
                   taACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACctct                                                                                                                                    >  1:287218/1‑150 (MQ=255)
                    aaCAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGAAAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTAGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCtt                                                                                                                                   >  1:423060/1‑150 (MQ=255)
                     aCAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTa                                                                                                                                  >  2:950593/1‑150 (MQ=255)
                        aTTGTGAGGATGATAGTGTTTAGAGTAAAGGGCAGGCATATATTGAACTCTTAAACGATAATTCTGATGATTATGGCTAAGAATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGt                                                                                                                               <  2:738534/150‑1 (MQ=255)
                        aTTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGt                                                                                                                               >  1:292894/1‑150 (MQ=255)
                         ttGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGtt                                                                                                                              <  1:322828/150‑1 (MQ=255)
                              aGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTTAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCtt                                                                                                                         >  2:1149314/1‑150 (MQ=255)
                              aGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCtt                                                                                                                         <  2:599831/150‑1 (MQ=255)
                              aGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCtt                                                                                                                         <  1:747201/150‑1 (MQ=255)
                               ggATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTa                                                                                                                        >  1:620967/1‑150 (MQ=255)
                                gatgatAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTaa                                                                                                                       <  1:876756/150‑1 (MQ=255)
                                  tgatAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAAt                                                                                                                     >  2:207106/1‑150 (MQ=255)
                                    atAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCa                                                                                                                   <  2:567725/150‑1 (MQ=255)
                                      aGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGc                                                                                                                 >  2:133741/1‑150 (MQ=255)
                                       ttgtTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCa                                                                                                                <  1:1183590/149‑1 (MQ=255)
                                         gtTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATc                                                                                                              >  1:412843/1‑150 (MQ=255)
                                           tAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAAATCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAG‑‑CACTATAAACAGCACCTCTTATGTTTTCTTAAATCAGCATCtt                                                                                                            >  2:457733/1‑150 (MQ=255)
                                             agagTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTaa                                                                                                          >  1:689918/1‑150 (MQ=255)
                                              gagTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTaaa                                                                                                         <  1:613002/150‑1 (MQ=255)
                                                gTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTaaaaa                                                                                                       >  1:395733/1‑149 (MQ=255)
                                                   aaGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGt                                                                                                    <  2:1249724/150‑1 (MQ=255)
                                                     gcgcAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTa                                                                                                  >  1:778799/1‑150 (MQ=255)
                                                     gcgcAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑AACTATAACCAGCACCTCTTATGTTTTATTAAATCAGCATCTTAAAACAGTTa                                                                                                  >  2:1146285/1‑150 (MQ=255)
                                                       gcAGGCATATATTGAACTCTTAAACGATTATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTTGTTATAGTGCAGTTAAC‑‑CACTATAACCAGAACATCTTATGTTTTCTTATATCAGAATCTTAAAACAGTTAag                                                                                                >  1:191227/1‑150 (MQ=255)
                                                        cAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAaga                                                                                               >  1:482745/1‑150 (MQ=255)
                                                            cATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCAt                                                                                           <  2:79666/150‑1 (MQ=255)
                                                                    gAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATaaa                                                                                   >  2:20397/1‑150 (MQ=255)
                                                                       ctctTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGTTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACATCTAATGTTTTATTAAATCAGCATCTTAAAACAGTAAATAGAAAAATATAAAtaa                                                                                >  2:1292850/1‑148 (MQ=255)
                                                                       ctctTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGATAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCAACTATTATGTTATCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACAATAAATCa                                                                                >  1:973244/1‑150 (MQ=255)
                                                                        tctTAAAAGATAATTCAGAAGATAATTTATAAGCATTACAATAATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCat                                                                               <  1:21954/150‑1 (MQ=255)
                                                                          ttAATCGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCatta                                                                             <  1:1110133/150‑1 (MQ=255)
                                                                           tAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTTGTTAAATTTGCTGTTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCattat                                                                            <  1:199329/150‑1 (MQ=255)
                                                                           tAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAACTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCattat                                                                            >  2:692467/1‑150 (MQ=255)
                                                                            aaaCGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAAAAGTTAAGAGCATTCTATAAATCattatt                                                                           >  1:628908/1‑150 (MQ=255)
                                                                             aaCGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTAttt                                                                          <  2:1316787/150‑1 (MQ=255)
                                                                                 aTAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCa                                                                      <  2:491754/150‑1 (MQ=255)
                                                                                 aTAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCa                                                                      >  1:258801/1‑150 (MQ=255)
                                                                                    aTTCATAAGATAATGTCTAATCATTACAATCATTTTTTTAAATTTTCTTGTTATAGTTCAGTTAAA‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATg                                                                   <  1:183220/150‑1 (MQ=255)
                                                                                    aTTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAATAGCATACTATAAATCATTATTTTTCAATg                                                                   >  1:161503/1‑150 (MQ=255)
                                                                                      tCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCg                                                                 <  2:882460/150‑1 (MQ=255)
                                                                                        agaagaTAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGtt                                                               >  2:1296924/1‑150 (MQ=255)
                                                                                         gaagaTAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATATTTCAATGCGTTa                                                              >  2:625302/1‑150 (MQ=255)
                                                                                         gaagaTAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGAATACTATAAATCATTATTTTTCAATGCGTTa                                                              >  2:489420/1‑150 (MQ=255)
                                                                                            gaTAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTagag                                                           <  2:863847/150‑1 (MQ=255)
                                                                                             aTAAAGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTTGAGGTAAC‑‑CACTATCACCAGGACCTCTTATGTTTTCTTTTATCTGCTTCTTAATACAGTTAAGAGCATACTATAAATCATTATTTTTCATTGCGTTAgaga                                                          <  1:666528/150‑1 (MQ=255)
                                                                                                 tGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGAGTTAGAGAaaaa                                                      >  2:607451/1‑150 (MQ=255)
                                                                                                  ggCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAAt                                                     <  2:1194333/150‑1 (MQ=255)
                                                                                                      aaGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTAc                                                 >  2:258247/1‑150 (MQ=255)
                                                                                                        ttaTTACATTCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑AACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACtt                                               <  1:116113/148‑1 (MQ=255)
                                                                                                                 tCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAAt                                      <  2:620967/150‑1 (MQ=255)
                                                                                                                 tCATTTGGTTAAATTTGATGGTTATAGTTCAGTTAAC‑‑CACTATAACCAGAAACTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAAt                                      <  1:319843/150‑1 (MQ=255)
                                                                                                                   aTTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTg                                    >  1:970378/1‑150 (MQ=255)
                                                                                                                   aTTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTg                                    <  1:950593/150‑1 (MQ=255)
                                                                                                                     ttGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGaa                                  <  1:1199690/150‑1 (MQ=255)
                                                                                                                     ttGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGaa                                  >  2:669422/1‑150 (MQ=255)
                                                                                                                       ggTTAAATTTGCTTGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATa                                <  1:963331/150‑1 (MQ=255)
                                                                                                                       ggTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAATCAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATa                                >  1:543270/1‑150 (MQ=255)
                                                                                                                          tAAATTTGCTGTTTATAGTGCAGTTAAC‑‑CACTATTACCAGAACCTCTTATTTTTTCTTAAATCAGCATCTTAATATAGTTATGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATAAgg                             <  1:632463/150‑1 (MQ=255)
                                                                                                                           aaaTTTGCTTGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATAAGGc                            <  2:809671/150‑1 (MQ=255)
                                                                                                                            aaTTTGCTTGTTATAGTGCAGTTAAC‑‑CACTATAAACAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATAAGGCt                           <  1:1268002/150‑1 (MQ=255)
                                                                                                                               ttGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATAAGGCTAtt                        <  1:657256/150‑1 (MQ=255)
                                                                                                                               ttGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGGTTTCTTAAATCAGCATCTTAAAACAGTTAACAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATAAGGCTAtt                        <  2:1143345/150‑1 (MQ=255)
                                                                                                                                tGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATAAGGCTAttt                       >  2:282353/1‑150 (MQ=255)
                                                                                                                                tGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGATTTCTTAAATCAGCATCTTAAAACAGGTAAGAGCATACTATAAATCATTATTTTTCAATGCGCTAGAGAACAAACTACTTTATCAAAAATGAATAAGGCTAttt                       <  2:41070/150‑1 (MQ=255)
                                                                                                                                tGCTGGTTATAGTG‑‑GTTAACTGCACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATAAGGCTAttt                       >  2:379854/1‑150 (MQ=39)
                                                                                                                                tGCTGGTTATAGTG‑‑GTTAACTGCACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATAAGGCTAttt                       >  2:1214072/1‑150 (MQ=39)
                                                                                                                                 gCTGGTTATAGTGCAGTTAAA‑‑CAATATAACCAGAAACTATTATGTTTTCTTAAATCATCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATAAGGCTATTTc                      <  1:216432/150‑1 (MQ=255)
                                                                                                                                  cTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATAAGGCTATTTCa                     >  2:475029/1‑150 (MQ=255)
                                                                                                                                  cTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATAAGGCTATTTCa                     <  2:1032225/150‑1 (MQ=255)
                                                                                                                                  cTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATAAGGCTATTTCa                     <  2:1091911/150‑1 (MQ=255)
                                                                                                                                  cTGGTTATAGTG‑‑GTTAACTGCACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTATTTTATCAAAATTGAATAAGGCTATTTCa                     >  1:311061/1‑150 (MQ=255)
                                                                                                                                   tGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATAAGGCTATTTCAg                    <  1:1292293/150‑1 (MQ=255)
                                                                                                                                    ggTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATAAGGCTATTTCAGa                   >  1:917386/1‑150 (MQ=255)
                                                                                                                                        ataGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATAAGGCTATTTCAGATTAc               <  1:1133732/150‑1 (MQ=255)
                                                                                                                                          aGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATAAGGCTATTTCAGATTACAt             >  1:1027435/1‑150 (MQ=255)
                                                                                                                                          aGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATAAGGCTATTTCAGATTACAt             >  1:598523/1‑150 (MQ=255)
                                                                                                                                           gTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACGATCTACTTTATCAAAATTGAATAAGGCTATTTCAGATTACAta            >  2:753780/1‑149 (MQ=255)
                                                                                                                                           gTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATAAGGCTATTTCAGATTACAta            >  1:468381/1‑149 (MQ=255)
                                                                                                                                           gTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATAAGGCTATTTCAGATTACATc            <  1:717829/150‑1 (MQ=255)
                                                                                                                                           gTGCAGTTAAC‑‑CACTATAACCAGCACCTCGTATGTTTTCTTAACTCAGCATCTTAAAACTGGTAAGTGCATACTATAACTGATTATTTTTCAATGCGTTAGAGAACAATCTAGTTTATCAAAATTGAATAAGGCTATTTCAGTTTTCATc            >  2:812532/1‑150 (MQ=255)
                                                                                                                                             gCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATAAGGCTATTTCAGATTACATCAc          >  1:178397/1‑150 (MQ=255)
                                                                                                                                                gTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATAC‑‑TAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATAAGGCTATTTCAGATTACATCACTGAAt     <  2:412843/150‑1 (MQ=255)
                                                                                                                                                  tAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATAAGGCTATTTCAGATTACATCACTGAAt     >  1:136542/1‑150 (MQ=255)
                                                                                                                                                  tAAC‑‑CACTATAAACAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATAAGGCTATTTCAGATTACATCACTGAAt     <  1:3142/150‑1 (MQ=255)
                                                                                                                                                     c‑‑cACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATAAGGCTATTTCAGATTACATCACTGAATTcc  <  1:207106/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
AATGTTTCTATCACATTATTAACAATTGTGAGGATGATAGTGTTAAGAGTAAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATCTACTTTATCAAAATTGAATAAGGCTATTTCAGATTACATCACTGAATTCC  >  NC_000913/2991656‑2991954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: