New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 444746465 (0.850)3 (0.040) 3/262 NT 4.5% coding (3353/3780 nt) tamB translocation and assembly module for autotransporter export, inner membrane subunit
?NC_000913 = 4447516 67 (0.930)coding (3405/3780 nt) tamB translocation and assembly module for autotransporter export, inner membrane subunit
Rejected: Frequency below/above cutoff threshold.

GGCAGATCAACATCCCTGAAGGGCGCTTCCATGCCTATGGTCAGGATCTGATTGTGCGTAAAGGTGAGTTACTGTTCTCTGGTCCGCCAGATCAACCGTATCTTAATATTGAAGCTATTCGTAACCCGGATGCTACAGAAGACGACGTAATCGCCGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4447308‑4447464
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑atcgccggGTCAGAGAAGATCTCCGCTTTCGGTTCGTCCGCCAGACCAGTGACGCGAACTCCGGCGATTACGTCGTCTTCTGTAGCATCCGGGTTACGAATAGCTTCAATATTAAGATACGGTTGATCTGGCGGACCAGAGAACAGTAACTCACCTT  <  NC_000913/4447516‑4447368
                                                                                                                                                                                                                                                                                                                  
GGCAGATCAACATCCCTGAAGGGCGCTTCCATGCCTATGGTCAGGATCTGATTGTGCGTAAAGGTGAGTTACTGTTCTCTGGTCCGCCAGATCAACCGTATCTTAATATTGAAGCTATTCGTAACCCGGATGCTACAGAAGACGACGTAA                                                                                                                                                              >  2:205397/1‑150
 GCAGATCAACATCCCTGAAGGGCGCTTCCATGCCTATGGTCAGGATCTGATTGTGCGTAAAGGTGAGTTACTGTTCTCTGGTCCGCCAGATCAACCGTATCTTAATATTGAAGCTATTCGTAACCCGGATGCTACAGAAGACGACGTAAT                                                                                                                                                             <  2:1188146/150‑1
    GATCAACATCCCTGAAGGGCGCTTCCATGCCTATGGTCAGGATCTGATTGTGCGTAAAGGTGAGTTACTGTTCTCTGGTCCGCCAGATCAACCGTATCTTAATATTGAAGCTATTCGTAACCCGGATGCTACAGAAGACGACGTAATCGC                                                                                                                                                          >  2:989914/1‑150
    GATCAACATCCCTGAAGGGCGCTTCCATGCCTATGGTCAGGATCTGATTGTGCGTAAAGGTGAGTTACTGTTCTCTGGTCCGCCAGATCAACCGTATCTTAATATTGAAGCTATTCGTAACCCGGATGCTACAGAAGACGACGTAATCGC                                                                                                                                                          >  2:279509/1‑150
    GATCAACATCCCTGAAGGGCGCTTCCATGCCTATGGTCAGGATCTGATTGTGCGTAAAGGTGAGTTACTGTTCTCTGGTCCGCCAGATCAACCGAATCTTAATATTGAAGCTATTCGTAACCCGGATGCTACAGAAGACGACGTAATCGC                                                                                                                                                          >  2:926715/1‑150
     ATCAACATCCCTGAAGGGCGCTTCCATGCCTATGGTCAGGATCTGATTGTGCGTAAAGGTGAGTTACTGTTCTCTGGTCCGCCAGATCAACCGTATCTTAATATTGAAGCTATTCGTAACCCGGATGCTACAGAAGACGACGTAATCGCC                                                                                                                                                         <  2:977895/150‑1
     ATCAACATCCCTGAAGGGCGCTTCCATGCCTATGGTCAGGATCTGATTGTGCGTAAAGGTGAGTTACTGTTCTCTGGTCCGCCAGATCAACCGTATCTTAATATTGAAGCTATTCGTAACCCGGATGCTACAGAAGACGACGTAATCGCC                                                                                                                                                         <  2:403143/150‑1
       CAACATCCCTGAAGGGCGCTTCCATGCCTATGGTCAGGATCTGATTGTGCGTAAAGGTGAGTTACTGTTCTCTGGTCCGCCAGATCAACCGTATCTTAATATTGAAGCTATTCGTAACCCGGATGCTACAGAAGACGACGTAATCGCCGG                                                                                                                                                       <  2:709719/150‑1
                                                                   GTTACTGTTCTCTGGTCCGCCAGATCAACCGTATCTTAATTTTGAAGCTATTCGTAACCCGGATGCTACAGAAGACGACGTAATCGCCGGGTCAGAGAAGATCTCCGCTTTCGGTTCGTCCGCCAGACCAGTGACGCGAACTCCGGCGAT                                                                                           <  2:454402/150‑1
                                                                       ccGTTCTCCGCTCCGCCAGATCACCCGTAGCTAACTCTCGAAGCGAGTCGTAACCCGGATGCTACAGAAGACGACGTAATCGCCGGGTCAGAGAAGATCTCCGCTGTCGGTTCGTCCGCCAGACCAGTGACGCGAACTCCGGCGATTACG                                                                                       <  1:529086/148‑1
                                                                          TTCTCTGGGCCGCCAGATCAACCGTATCTAAATATTGAAGCTATTCGTAACCCGGATGCTACAGAAGACGACGTAATCGCCGGGTCAGAGAAGATCTCCGCTTTCGGTTCGTCCGCCAGACCAGTGACGCGAACTCCGGCGATTACGTCG                                                                                    <  1:1211834/150‑1
                                                                                                                                                     ATCGCCGGGTCAGAGAAGATCTCCGCTGTCGGTTCTTCCGCCAGACCAGGTACGCCAACTCCGGCGATTACGTCGTCTTCTGTAGCATCCGGGTTACGAATAGCTTCAATATTAAGATACGGTTGATCTGGCGGACCAGAGAACAGTAAC         <  1:99665/150‑1
                                                                                                                                                      TCGCCGGGTCAGAGAAGATCTCCGCTTTCGGTTCGTCCGCCAGACCAGTGACGCGAACTCCGGCGATTACGTCGTCTTCTGTAGCATCCGGGTTACGAATAGCTTCAATATTAAGATACGGTTGATCTGGCGGACCAGAGAACAGTAACT        <  2:246674/150‑1
                                                                                                                                                       CGCCGGGTCAGAGAAGATCTCCGCTTTCGGTTCGTCCGCCAGACCAGTGACGCGAACTCCGGCGATTACGTCGTCTTCTGTAGCATCCGGGTTACGAATAGCTTCAATATTTAGATACGGTTGATCTGGCGGACCAGCGCACAGTAACTC       >  2:982055/1‑150
                                                                                                                                                       CGCCGGGTCAGAGAAGATCTCCGCTTTCGGTTCGTCCGCCAGACCAGTGACGCGAACTCCGGCGATTACGTCGGCTTCTGTAGCATCCGGGTTACGAATAGCTTCAATATTAAGATACGGTTGATCTGGCGGACCAGAGAACAGTAACTC       <  1:293139/150‑1
                                                                                                                                                          CGGGTCAGAGAAGATCTCCGCTTTCGGTTCGTCCGCCAGACCAGTGACGCGAACTCCGGCGATTACGTCGTCTTCTGTAGCATCCGGGTTACGAATAGCTTCAATATTAAGATACGGTTGATCTGGCGGACCAGAGAACAGTAACTCACC    <  1:725995/150‑1
                                                                                                                                                          CGGGTCAGAGAAGATCTCCGCTTTCGGTTCGTCCGCCAGACCAGTGACGCGAACTCCGGCGATTACGTCGTCTTCTGTAGCATCCGGGTTACGAATAGCTTCAATATTAAGATACGGTTGATCTGGCGGACCAGAGAACAGTAACTCACC    >  2:978464/1‑150
                                                                                                                                                          CGGGTCAGAGAAGATCTCCGCGTTCGGTTCGTCCGCCAGACCAGTGACGCGAACTCCGGCGATTACGTCGTCTGCTGTAGCATCCGGGTTACGAATAGCTTCAATATTAAGATACGGGTGATCTGGCGGACCAGAGAACAGTAACTCACC    <  2:224767/150‑1
                                                                                                                                                           GGGTCAGAGAAGATCTCCGCTTTCGGTTCGTCCGCCAGACCAGTGACGCGAACTCCGGCGATTACGTCGTCTTCTGTAGCATCCGGGTTACGAATAGCTTCAATATTAAGATACGGTTTATCTGGCGGACCAGAGAACAGTAACTCACat   >  1:604940/1‑148
                                                                                                                                                            GGTCATAGAAGATCTCCGCTTTCGGTTCGTCCTCCAGACCAGTGACGCGAACTCCGGCGATTACGGCGTCTTCTTTAGCATCCGGGTTACGAATAGCTTCAATATTAAGATACGGTTGATCTGGCGGACCAGAGAACAGTAACTCACCTT  <  2:41919/150‑1
                                                                                                                                                            GGTCAGAGAAGATCTCCGCTTTCGGTTCGTCCGCCAGACCAGTGACGCGAACTCCGGCGATTACGTCGTCTTCTGTAGCATCCGGGTTACGAATAGCTTCAATATTAAGATACGGTTGATCTGGCGGACCAGAGAACAGTAACTCACCTT  >  2:136254/1‑150
                                                                                                                                                                                                                                                                                                                  
GGCAGATCAACATCCCTGAAGGGCGCTTCCATGCCTATGGTCAGGATCTGATTGTGCGTAAAGGTGAGTTACTGTTCTCTGGTCCGCCAGATCAACCGTATCTTAATATTGAAGCTATTCGTAACCCGGATGCTACAGAAGACGACGTAATCGCCGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4447308‑4447464
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑atcgccggGTCAGAGAAGATCTCCGCTTTCGGTTCGTCCGCCAGACCAGTGACGCGAACTCCGGCGATTACGTCGTCTTCTGTAGCATCCGGGTTACGAATAGCTTCAATATTAAGATACGGTTGATCTGGCGGACCAGAGAACAGTAACTCACCTT  <  NC_000913/4447516‑4447368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.