Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,504,884 T→C 6.9% intergenic (+19/+21) insQ → / ← ydcO IS609 transposase B/BenE family inner membrane putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,504,8840TC 6.9% 209.6 / 5.9 74intergenic (+19/+21)insQ/ydcOIS609 transposase B/BenE family inner membrane putative transporter
Reads supporting (aligned to +/- strand):  ref base T (28/39);  new base C (2/3);  total (32/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.01e-01

CGAACGCCGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGCCACCA  >  NC_000913/1504735‑1505032
                                                                                                                                                     |                                                                                                                                                    
cGAACGCCGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGt                                                                                                                                                      <  2:386560/150‑1 (MQ=255)
  accGCCGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACACACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTcc                                                                                                                                                    <  1:322435/148‑1 (MQ=255)
     gCCGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTc                                                                                                                                                 <  2:220558/150‑1 (MQ=255)
        gATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCAGAACGTAGCAGGGATCCACGTCCTTCAgg                                                                                                                                              >  1:315156/1‑150 (MQ=255)
        gATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCATGTGGAGAGATGGTGCATTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAgg                                                                                                                                              <  1:836886/150‑1 (MQ=255)
         aTGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAggg                                                                                                                                             <  2:77641/150‑1 (MQ=255)
           gTAAAGGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCg                                                                                                                                           <  2:72648/150‑1 (MQ=255)
             aaaTGGCGCTCGCAAGATTTTAGCGGCGGGGCACGCCGGTCTTGCCTGTGGAGAGATGGTGCCGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGCCCTGAAGGACGTg                                                                                                                                         <  2:954793/150‑1 (MQ=255)
             aaaTCGCGCTAGCAAGATTTTCGCGTCGGGGCACGCCGTGCTTGCCTGGGCAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGGAGGACCCCACCGAAATGAATCAGGCGACAGCCTGATCGTAGGAGGGTTCCTCGTCCTTGAGGGCGTg                                                                                                                                         <  2:194937/150‑1 (MQ=255)
               aTGGCACTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTgga                                                                                                                                       <  2:242283/150‑1 (MQ=255)
                  gcgcTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGACCCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGCCCTGAAGGACGTggatcc                                                                                                                                    <  1:1285063/150‑4 (MQ=255)
                  gcgcTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTggaggt                                                                                                                                    >  1:248212/1‑149 (MQ=255)
                  gcgcTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGCCCTGAAGGACGTggatcc                                                                                                                                    <  1:1290052/150‑4 (MQ=255)
                     cTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGACCTTCAGGTCGTGGAGGATgg                                                                                                                                 >  2:703501/1‑149 (MQ=255)
                      ccGCAACAGGTTCGCGCCGGGGCACCCCGCGCTGCCCGGCGGCGAGCTGGTGCAGTCCCGCCGCCCGTTGAACCAGGACCCCCCCGAAATGATTCCGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTc                                                                                                                                <  2:928932/149‑1 (MQ=255)
                        gCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGATGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCaa                                                                                                                              <  1:463191/150‑1 (MQ=255)
                          aaCATTTAAGCGGCGGGGCACGCAGTTCTTGCCTGTGGAGGGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGATCCCACCCAAATGATTCAGGCGACCGCCTGAACGTAGCAGGCATCCACGTCCTTCAGGCCGAGGAGGATGTCAata                                                                                                                            >  2:679712/1‑150 (MQ=255)
                              ttttAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCgg                                                                                                                        <  1:447297/150‑1 (MQ=255)
                              ttttAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATAATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCgg                                                                                                                        >  1:24552/1‑150 (MQ=255)
                                ttAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGtt                                                                                                                      <  1:283087/150‑1 (MQ=255)
                                  aGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTc                                                                                                                    <  2:994816/150‑1 (MQ=255)
                                  aGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTGGAAGCAGGAACCCACCGAAAGGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTc                                                                                                                    <  2:988970/150‑1 (MQ=255)
                                    cggcggGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTg                                                                                                                  >  2:236197/1‑150 (MQ=255)
                                         gggCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATACAGGCGACAGCCTGAACGTAGCAGGGAACCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTTTCAGc                                                                                                             >  2:932589/1‑150 (MQ=255)
                                             aCGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGGCCTGCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGAtt                                                                                                         >  1:334871/1‑150 (MQ=255)
                                              cGCCGTTCTTGCCTGTCGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTa                                                                                                        <  2:517818/150‑1 (MQ=255)
                                                   ttCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTc                                                                                                   <  1:712638/150‑1 (MQ=255)
                                                   ttCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTc                                                                                                   <  1:1299896/150‑1 (MQ=255)
                                                    tCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCa                                                                                                  <  1:836818/150‑1 (MQ=255)
                                                       tGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAaca                                                                                               >  1:414408/1‑150 (MQ=255)
                                                            gtgGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATTTCGGTTTCTGTCAGCGATTAAATTCAACACCACg                                                                                          >  2:803901/1‑150 (MQ=255)
                                                              ggAGAGATGGTGCAGTCAGGCCGCCCGATGAAGCAGGAACACACCGAAATGATTCAGGCTACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATAGGTTACAGTAAGCGATAACATTCAACACCACGTa                                                                                        >  1:76048/1‑150 (MQ=255)
                                                                agagaTGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAAc                                                                                      >  1:1233626/1‑150 (MQ=255)
                                                                     tGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACg                                                                                 >  1:167683/1‑150 (MQ=255)
                                                                     tGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACg                                                                                 <  2:792199/150‑1 (MQ=255)
                                                                            gTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCgg                                                                          >  2:1109718/1‑150 (MQ=255)
                                                                              cAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCa                                                                        >  2:1189409/1‑150 (MQ=255)
                                                                                ggCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAAt                                                                      <  2:223441/150‑1 (MQ=255)
                                                                                ggCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAAt                                                                      <  1:1036481/150‑1 (MQ=255)
                                                                                 gccgccCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAAtt                                                                     >  2:531249/1‑150 (MQ=255)
                                                                                  ccgccCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTa                                                                    <  2:565508/150‑1 (MQ=255)
                                                                                   cgccCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGATTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTaa                                                                   <  1:139496/150‑1 (MQ=255)
                                                                                       cGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATccc                                                               <  2:1020022/150‑1 (MQ=255)
                                                                                       cGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATccc                                                               >  2:907790/1‑150 (MQ=255)
                                                                                          tGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCaa                                                            <  2:337364/150‑1 (MQ=255)
                                                                                           gAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAAGTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCaaa                                                           >  2:18022/1‑150 (MQ=255)
                                                                                             aGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAAc                                                         <  1:998209/150‑1 (MQ=255)
                                                                                                         ccGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATc                                             <  1:977434/150‑1 (MQ=255)
                                                                                                           gAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATccc                                           >  2:522820/1‑150 (MQ=255)
                                                                                                             aaTGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGa                                         <  1:236197/150‑1 (MQ=255)
                                                                                                               tGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGAcc                                       <  2:992845/150‑1 (MQ=255)
                                                                                                                   tCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCg                                   <  2:764200/150‑1 (MQ=255)
                                                                                                                   tCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCg                                   <  2:1267160/150‑1 (MQ=255)
                                                                                                                    cAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGTTGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGt                                  >  2:1290238/1‑150 (MQ=255)
                                                                                                                    cAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGt                                  <  1:1203919/150‑1 (MQ=255)
                                                                                                                     tggCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTc                                 <  2:207948/149‑1 (MQ=255)
                                                                                                                     aGGCGAGAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGGTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTc                                 <  2:826948/150‑1 (MQ=255)
                                                                                                                      ggCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAACCGCAGAACCAATCCCGACCAGCGTCa                                >  2:1095079/1‑150 (MQ=255)
                                                                                                                        ctgtcgCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCaaa                              >  1:972076/6‑149 (MQ=255)
                                                                                                                         gACAGCCTGAACGTAGCAGGGATCCCCGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACCCCACGTAACAAACGCCTCCGGCAATTAAGCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATc                             >  1:1055998/1‑150 (MQ=255)
                                                                                                                              ccTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACtt                        >  2:280584/1‑150 (MQ=255)
                                                                                                                               cTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTg                       <  2:131633/150‑1 (MQ=255)
                                                                                                                                        gCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCa              <  2:1039718/150‑1 (MQ=255)
                                                                                                                                        gCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCa              <  1:522820/150‑1 (MQ=255)
                                                                                                                                          aGGGATCCACGTCCTTCTGTGCGTGGAGGATGTCTATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGa            <  2:798515/150‑1 (MQ=255)
                                                                                                                                          aGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCCCTTGCCGTTACCAGa            >  2:317992/1‑150 (MQ=255)
                                                                                                                                           gggATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCCGCGTCAATCCACTTGCCGGTACCCGaa           >  1:680529/1‑150 (MQ=255)
                                                                                                                                            ggATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGaaa          <  2:315156/150‑1 (MQ=255)
                                                                                                                                              ctCCACGCCCTGAAGGACGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATg        >  1:525722/2‑150 (MQ=255)
                                                                                                                                                ccACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTGCCAGAAATGcc      >  1:979398/1‑150 (MQ=255)
                                                                                                                                                ccACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGcc      <  2:167683/150‑1 (MQ=255)
                                                                                                                                                ccACGCCCTGAAGGACGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGcc      >  2:166191/1‑150 (MQ=255)
                                                                                                                                                 cACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATGCAACACCACGTAACCAACGCCGCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTGTCCGTTACCAGAAATGcgc     >  2:1269640/1‑148 (MQ=255)
                                                                                                                                                    gTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCTGAACCAATCCCGACCAGCGTCAATCCACTGGCCGTTACCAGAAATGccaccc  >  2:106892/1‑149 (MQ=255)
                                                                                                                                                    gTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGccaccc  >  1:39356/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CGAACGCCGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGCCACCA  >  NC_000913/1504735‑1505032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: