Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,530,496 G→T 12.2% intergenic (+92/‑90) ydcD → / → yncI putative immunity protein for RhsE/pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,530,4960GT12.2% 142.7 / 9.4 63intergenic (+92/‑90)ydcD/yncIputative immunity protein for RhsE/pseudogene
Reads supporting (aligned to +/- strand):  ref base G (33/22);  new base T (3/5);  total (36/27)
Fisher's exact test for biased strand distribution p-value = 2.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.16e-01

ATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACGACAACAAGGA  >  NC_000913/1530349‑1530645
                                                                                                                                                   |                                                                                                                                                     
attatGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACACAACCAGAAAGATAAAAATAAAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTa                                                                                                                                                     >  2:822407/1‑150 (MQ=255)
attatGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTa                                                                                                                                                     >  2:82145/1‑150 (MQ=255)
attatGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTa                                                                                                                                                     >  2:228692/1‑150 (MQ=255)
 ttatGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTaa                                                                                                                                                    <  2:634517/150‑1 (MQ=255)
    tGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACCCCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATc                                                                                                                                                 <  1:925676/150‑1 (MQ=255)
    tGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATc                                                                                                                                                 >  2:1095/1‑150 (MQ=255)
         aCAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATCGTGATTTACATCACAAt                                                                                                                                            <  1:701809/150‑1 (MQ=255)
          aaaTTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATTAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGAtt                                                                                                                                           <  2:332484/149‑1 (MQ=255)
           aaTTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATAAAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATTTACATCACAAttt                                                                                                                                          <  1:716185/150‑1 (MQ=255)
             tttGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTc                                                                                                                                        <  1:498408/150‑1 (MQ=255)
              ttGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATTTACATCACAATTTTCa                                                                                                                                       <  1:857369/150‑1 (MQ=255)
               tGAAAAATGAAGTTTTTGACTGTATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATCGTGATTTACATCACAATTTTCAt                                                                                                                                      <  1:30600/150‑1 (MQ=255)
               tGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCAt                                                                                                                                      >  1:444752/1‑150 (MQ=255)
               tGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCAt                                                                                                                                      >  2:270253/1‑150 (MQ=255)
                taaaaaTGAAGTTTTTGACTGGATTGGTAAATGGTAATAATGAACAACACCATAAATAGAAAAATATAAAATACATACAGAAAGACATTAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATCGTGATTTACATCACAATTTTCAtg                                                                                                                                     <  1:231634/149‑2 (MQ=255)
                  aaaaTGAAGTTTTTTACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCtt                                                                                                                                   <  1:887175/150‑1 (MQ=255)
                     aTGAAGTTTTTGACTGTATTGGAAAATGGTCATAATCAACAACACCAGAAAGAGAAAAATATAAAAAACAAACAGAAAGACATCAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGc                                                                                                                                <  1:1156350/150‑1 (MQ=255)
                      tGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCt                                                                                                                               >  1:1012045/1‑150 (MQ=255)
                      tGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGAAGGGGAAGGGCAAGATCATGAAAATTGTGATTTAAATCACGATTTTCATCTTTGCt                                                                                                                               >  1:1017271/1‑150 (MQ=255)
                      tGAAGTTTTTGACTGGATTGGAAAATGTTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAACGACATGAGAGCAAGGGCCGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCt                                                                                                                               <  1:940865/150‑1 (MQ=255)
                        aaGTTTTTGACTGGATTGGTAAATGGTCATAATCAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCttt                                                                                                                             <  2:607059/150‑1 (MQ=255)
                         aGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTa                                                                                                                            >  2:427712/1‑150 (MQ=255)
                          gTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAATGACATGATAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTaa                                                                                                                           >  1:203080/1‑150 (MQ=255)
                           tttttGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAAAATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAAc                                                                                                                          <  2:1060962/150‑1 (MQ=255)
                               tGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCt                                                                                                                      >  1:687212/1‑150 (MQ=255)
                                gACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTa                                                                                                                     >  2:704124/1‑150 (MQ=255)
                                          gTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCa                                                                                                           >  1:659269/1‑150 (MQ=255)
                                           tAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCaa                                                                                                          >  2:770790/1‑150 (MQ=255)
                                             aaTGGTCATAATGCACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGATCAAGTGACGGGGCAGTGCAATATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCaaaa                                                                                                        <  1:921587/150‑1 (MQ=255)
                                                   cATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAATATTGTGATGTAAAGCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAAc                                                                                                  <  2:703348/150‑1 (MQ=255)
                                                    aTAATTAACAACACCAGAAAGATAAAAATTTAAAATAAATACAGAAATACTTTAGAGCAAGGGACGGGGAAGTGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAAcc                                                                                                 <  1:579921/150‑1 (MQ=255)
                                                         gAACAACCCCAGAAAGCGAAAAATATAAAATACAAACAGAAAGACATGAGAGCAAGCGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTgg                                                                                            <  2:119005/150‑1 (MQ=255)
                                                           acaacaCCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGaa                                                                                          >  1:684713/1‑150 (MQ=255)
                                                              acacCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACATTTGGAATaa                                                                                       >  2:236889/1‑150 (MQ=255)
                                                               cacCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAAc                                                                                      <  2:511451/150‑1 (MQ=255)
                                                                      aaGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAg                                                                               >  2:706050/1‑150 (MQ=255)
                                                                        gagaAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGAt                                                                             <  2:692223/150‑1 (MQ=255)
                                                                               ttataAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATAATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTAAACCTTTGGAATAACTTAAAATATAAAAAAg                                                                      >  1:40063/2‑150 (MQ=255)
                                                                               atatAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATAAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAg                                                                      >  2:1208606/1‑150 (MQ=255)
                                                                                   aaaaTACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACg                                                                  >  1:554959/1‑150 (MQ=255)
                                                                                         catacaGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGt                                                            >  2:715937/1‑150 (MQ=255)
                                                                                              aGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTa                                                       >  2:170807/1‑150 (MQ=255)
                                                                                               gAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGCCGCAAGGTGAGTAt                                                      >  1:1041345/1‑150 (MQ=255)
                                                                                                  aGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTACCGCCTTCAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCATGTGAGTATTCa                                                   >  2:58102/1‑150 (MQ=255)
                                                                                                  aGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTACCGCCTACAGGTGATCAAAATTTAACCTTTCGAATAACTAAAAAGAGAATAAAGGACGCCAGGTGAGTATTCa                                                   >  1:628135/1‑150 (MQ=255)
                                                                                                     cATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAg                                                <  1:39901/150‑1 (MQ=255)
                                                                                                     cATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTAATCTTTGATTTAACGCCTACAGTTGATCAAAATTTAACCTTTGGATTAACTAAAAAGATAAAAAAGGACGCAAGGTGAGTATTCAAAg                                                >  1:984113/1‑150 (MQ=255)
                                                                                                           agCAAGGGACGGGTCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCt                                          <  2:820667/150‑1 (MQ=255)
                                                                                                              aaGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGa                                       >  1:3465/1‑150 (MQ=255)
                                                                                                                  gACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATtat                                   >  2:1205552/1‑150 (MQ=255)
                                                                                                                  gACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATtat                                   >  1:263771/1‑150 (MQ=255)
                                                                                                                    cGGGGCAGGGCAAGTTAATTAAAATTGTGATGTAAATAACGATTTTCATATTTGCTTTAACGCCTACAGGTGATCAAAATTTAAACTTTGGAATAACTAAAAATATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTatat                                 <  2:1017271/150‑1 (MQ=255)
                                                                                                                    cGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTatat                                 <  2:1012045/150‑1 (MQ=255)
                                                                                                                       ggcagggcaAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTc                              >  1:51116/1‑150 (MQ=255)
                                                                                                                        gcagggcaAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCa                             <  1:82145/150‑1 (MQ=255)
                                                                                                                        gcagggcaAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCa                             <  1:228692/150‑1 (MQ=255)
                                                                                                                            ggcaAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGa                         >  2:471412/1‑150 (MQ=255)
                                                                                                                                caaagaTGAAAATCGTGATTTACATCACAATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGAcccc                     >  1:471553/6‑150 (MQ=39)
                                                                                                                                    cATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGTTGATAAAAATTTAAAATTTGTAATAAATAAAAAGATAAAAAATGAAGAAATTTAAGTATTCAAAGTTTGCTTGATTATATTTCATTGAACACTGat                 >  2:107934/1‑150 (MQ=255)
                                                                                                                                     aTGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGata                >  1:588774/1‑150 (MQ=255)
                                                                                                                                     aTGAAAATCGTGATTTACATCACAATTTTCTTCTTTGGTTTACCGCCTACAGGTGATCATATTTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGGGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGata                >  1:864673/1‑150 (MQ=255)
                                                                                                                                      tGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGatat               <  2:588774/150‑1 (MQ=255)
                                                                                                                                                gATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACGAcaacaa     >  2:195175/1‑150 (MQ=255)
                                                                                                                                                   gTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACGACAACAAGGa  >  1:1238648/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
ATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACGACAACAAGGA  >  NC_000913/1530349‑1530645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: