Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,530,499 A→C 11.7% intergenic (+95/‑87) ydcD → / → yncI putative immunity protein for RhsE/pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,530,4990AC11.7% 143.6 / 12.5 60intergenic (+95/‑87)ydcD/yncIputative immunity protein for RhsE/pseudogene
Reads supporting (aligned to +/- strand):  ref base A (32/21);  new base C (2/5);  total (34/26)
Fisher's exact test for biased strand distribution p-value = 2.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.80e-01

TTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACGACAACAAGGAAAG  >  NC_000913/1530350‑1530648
                                                                                                                                                     |                                                                                                                                                     
ttatGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTaa                                                                                                                                                       <  2:634517/150‑1 (MQ=255)
   tGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACCCCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATc                                                                                                                                                    <  1:925676/150‑1 (MQ=255)
   tGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATc                                                                                                                                                    >  2:1095/1‑150 (MQ=255)
        aCAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATCGTGATTTACATCACAAt                                                                                                                                               <  1:701809/150‑1 (MQ=255)
         aaaTTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATTAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGAtt                                                                                                                                              <  2:332484/149‑1 (MQ=255)
          aaTTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATAAAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATTTACATCACAAttt                                                                                                                                             <  1:716185/150‑1 (MQ=255)
            tttGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTc                                                                                                                                           <  1:498408/150‑1 (MQ=255)
             ttGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATTTACATCACAATTTTCa                                                                                                                                          <  1:857369/150‑1 (MQ=255)
              tGAAAAATGAAGTTTTTGACTGTATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATCGTGATTTACATCACAATTTTCAt                                                                                                                                         <  1:30600/150‑1 (MQ=255)
              tGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCAt                                                                                                                                         >  2:270253/1‑150 (MQ=255)
              tGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCAt                                                                                                                                         >  1:444752/1‑150 (MQ=255)
               taaaaaTGAAGTTTTTGACTGGATTGGTAAATGGTAATAATGAACAACACCATAAATAGAAAAATATAAAATACATACAGAAAGACATTAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATCGTGATTTACATCACAATTTTCAtg                                                                                                                                        <  1:231634/149‑2 (MQ=255)
                 aaaaTGAAGTTTTTTACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCtt                                                                                                                                      <  1:887175/150‑1 (MQ=255)
                    aTGAAGTTTTTGACTGTATTGGAAAATGGTCATAATCAACAACACCAGAAAGAGAAAAATATAAAAAACAAACAGAAAGACATCAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGc                                                                                                                                   <  1:1156350/150‑1 (MQ=255)
                     tGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCt                                                                                                                                  >  1:1012045/1‑150 (MQ=255)
                     tGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGAAGGGGAAGGGCAAGATCATGAAAATTGTGATTTAAATCACGATTTTCATCTTTGCt                                                                                                                                  >  1:1017271/1‑150 (MQ=255)
                     tGAAGTTTTTGACTGGATTGGAAAATGTTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAACGACATGAGAGCAAGGGCCGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCt                                                                                                                                  <  1:940865/150‑1 (MQ=255)
                       aaGTTTTTGACTGGATTGGTAAATGGTCATAATCAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCttt                                                                                                                                <  2:607059/150‑1 (MQ=255)
                        aGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTa                                                                                                                               >  2:427712/1‑150 (MQ=255)
                         gTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAATGACATGATAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTaa                                                                                                                              >  1:203080/1‑150 (MQ=255)
                          tttttGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAAAATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAAc                                                                                                                             <  2:1060962/150‑1 (MQ=255)
                              tGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCt                                                                                                                         >  1:687212/1‑150 (MQ=255)
                               gACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTa                                                                                                                        >  2:704124/1‑150 (MQ=255)
                                         gTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCa                                                                                                              >  1:659269/1‑150 (MQ=255)
                                          tAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCaa                                                                                                             >  2:770790/1‑150 (MQ=255)
                                            aaTGGTCATAATGCACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGATCAAGTGACGGGGCAGTGCAATATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCaaaa                                                                                                           <  1:921587/150‑1 (MQ=255)
                                                  cATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAATATTGTGATGTAAAGCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAAc                                                                                                     <  2:703348/150‑1 (MQ=255)
                                                   aTAATTAACAACACCAGAAAGATAAAAATTTAAAATAAATACAGAAATACTTTAGAGCAAGGGACGGGGAAGTGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAAcc                                                                                                    <  1:579921/150‑1 (MQ=255)
                                                        gAACAACCCCAGAAAGCGAAAAATATAAAATACAAACAGAAAGACATGAGAGCAAGCGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTgg                                                                                               <  2:119005/150‑1 (MQ=255)
                                                          acaacaCCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGaa                                                                                             >  1:684713/1‑150 (MQ=255)
                                                             acacCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACATTTGGAATaa                                                                                          >  2:236889/1‑150 (MQ=255)
                                                              cacCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAAc                                                                                         <  2:511451/150‑1 (MQ=255)
                                                                     aaGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAg                                                                                  >  2:706050/1‑150 (MQ=255)
                                                                       gagaAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGAt                                                                                <  2:692223/150‑1 (MQ=255)
                                                                              ttataAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATAATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTAAACCTTTGGAATAACTTAAAATATAAAAAAg                                                                         >  1:40063/2‑150 (MQ=255)
                                                                              atatAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATAAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAg                                                                         >  2:1208606/1‑150 (MQ=255)
                                                                                  aaaaTACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACg                                                                     >  1:554959/1‑150 (MQ=255)
                                                                                        catacaGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGt                                                               >  2:715937/1‑150 (MQ=255)
                                                                                             aGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTa                                                          >  2:170807/1‑150 (MQ=255)
                                                                                              gAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGCCGCAAGGTGAGTAt                                                         >  1:1041345/1‑150 (MQ=255)
                                                                                                 aGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTACCGCCTTCAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCATGTGAGTATTCa                                                      >  2:58102/1‑150 (MQ=255)
                                                                                                 aGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTACCGCCTACAGGTGATCAAAATTTAACCTTTCGAATAACTAAAAAGAGAATAAAGGACGCCAGGTGAGTATTCa                                                      >  1:628135/1‑150 (MQ=255)
                                                                                                    cATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAg                                                   <  1:39901/150‑1 (MQ=255)
                                                                                                    cATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTAATCTTTGATTTAACGCCTACAGTTGATCAAAATTTAACCTTTGGATTAACTAAAAAGATAAAAAAGGACGCAAGGTGAGTATTCAAAg                                                   >  1:984113/1‑150 (MQ=255)
                                                                                                          agCAAGGGACGGGTCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCt                                             <  2:820667/150‑1 (MQ=255)
                                                                                                             aaGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGa                                          >  1:3465/1‑150 (MQ=255)
                                                                                                                 gACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATtat                                      >  2:1205552/1‑150 (MQ=255)
                                                                                                                 gACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATtat                                      >  1:263771/1‑150 (MQ=255)
                                                                                                                   cGGGGCAGGGCAAGTTAATTAAAATTGTGATGTAAATAACGATTTTCATATTTGCTTTAACGCCTACAGGTGATCAAAATTTAAACTTTGGAATAACTAAAAATATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTatat                                    <  2:1017271/150‑1 (MQ=255)
                                                                                                                   cGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTatat                                    <  2:1012045/150‑1 (MQ=255)
                                                                                                                      ggcagggcaAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTc                                 >  1:51116/1‑150 (MQ=255)
                                                                                                                       gcagggcaAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCa                                <  1:228692/150‑1 (MQ=255)
                                                                                                                       gcagggcaAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCa                                <  1:82145/150‑1 (MQ=255)
                                                                                                                           ggcaAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGa                            >  2:471412/1‑150 (MQ=255)
                                                                                                                               caaagaTGAAAATCGTGATTTACATCACAATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGAcccc                        >  1:471553/6‑150 (MQ=39)
                                                                                                                                   cATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGTTGATAAAAATTTAAAATTTGTAATAAATAAAAAGATAAAAAATGAAGAAATTTAAGTATTCAAAGTTTGCTTGATTATATTTCATTGAACACTGat                    >  2:107934/1‑150 (MQ=255)
                                                                                                                                    aTGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGata                   >  1:588774/1‑150 (MQ=255)
                                                                                                                                    aTGAAAATCGTGATTTACATCACAATTTTCTTCTTTGGTTTACCGCCTACAGGTGATCATATTTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGGGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGata                   >  1:864673/1‑150 (MQ=255)
                                                                                                                                     tGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGatat                  <  2:588774/150‑1 (MQ=255)
                                                                                                                                               gATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACGAcaacaa        >  2:195175/1‑150 (MQ=255)
                                                                                                                                                  gTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACGACAACAAGGa     >  1:1238648/1‑150 (MQ=255)
                                                                                                                                                    aaaTCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACGTCAACAAGGaaa   >  1:1077792/1‑150 (MQ=255)
                                                                                                                                                     aaTCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACGACAACAAGGAAAg  <  1:69161/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACGACAACAAGGAAAG  >  NC_000913/1530350‑1530648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: