Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,530,505 G→A 11.3% intergenic (+101/‑81) ydcD → / → yncI putative immunity protein for RhsE/pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,530,5050GA11.3% 158.3 / 11.5 62intergenic (+101/‑81)ydcD/yncIputative immunity protein for RhsE/pseudogene
Reads supporting (aligned to +/- strand):  ref base G (33/22);  new base A (2/5);  total (35/27)
Fisher's exact test for biased strand distribution p-value = 2.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.05e-01

ACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACGACAACAAGGAAAGGTTA  >  NC_000913/1530358‑1530652
                                                                                                                                                   |                                                                                                                                                   
aCAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATCGTGATTTACATCACAAt                                                                                                                                                   <  1:701809/150‑1 (MQ=255)
 aaaTTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATTAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGAtt                                                                                                                                                  <  2:332484/149‑1 (MQ=255)
  aaTTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATAAAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATTTACATCACAAttt                                                                                                                                                 <  1:716185/150‑1 (MQ=255)
    tttGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTc                                                                                                                                               <  1:498408/150‑1 (MQ=255)
     ttGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATTTACATCACAATTTTCa                                                                                                                                              <  1:857369/150‑1 (MQ=255)
      tGAAAAATGAAGTTTTTGACTGTATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATCGTGATTTACATCACAATTTTCAt                                                                                                                                             <  1:30600/150‑1 (MQ=255)
      tGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCAt                                                                                                                                             >  2:270253/1‑150 (MQ=255)
      tGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCAt                                                                                                                                             >  1:444752/1‑150 (MQ=255)
       taaaaaTGAAGTTTTTGACTGGATTGGTAAATGGTAATAATGAACAACACCATAAATAGAAAAATATAAAATACATACAGAAAGACATTAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATCGTGATTTACATCACAATTTTCAtg                                                                                                                                            <  1:231634/149‑2 (MQ=255)
         aaaaTGAAGTTTTTTACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCtt                                                                                                                                          <  1:887175/150‑1 (MQ=255)
            aTGAAGTTTTTGACTGTATTGGAAAATGGTCATAATCAACAACACCAGAAAGAGAAAAATATAAAAAACAAACAGAAAGACATCAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGc                                                                                                                                       <  1:1156350/150‑1 (MQ=255)
             tGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCt                                                                                                                                      >  1:1012045/1‑150 (MQ=255)
             tGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGAAGGGGAAGGGCAAGATCATGAAAATTGTGATTTAAATCACGATTTTCATCTTTGCt                                                                                                                                      >  1:1017271/1‑150 (MQ=255)
             tGAAGTTTTTGACTGGATTGGAAAATGTTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAACGACATGAGAGCAAGGGCCGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCt                                                                                                                                      <  1:940865/150‑1 (MQ=255)
               aaGTTTTTGACTGGATTGGTAAATGGTCATAATCAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCttt                                                                                                                                    <  2:607059/150‑1 (MQ=255)
                aGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTa                                                                                                                                   >  2:427712/1‑150 (MQ=255)
                 gTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAATGACATGATAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTaa                                                                                                                                  >  1:203080/1‑150 (MQ=255)
                  tttttGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAAAATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAAc                                                                                                                                 <  2:1060962/150‑1 (MQ=255)
                      tGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCt                                                                                                                             >  1:687212/1‑150 (MQ=255)
                       gACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTa                                                                                                                            >  2:704124/1‑150 (MQ=255)
                                 gTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCa                                                                                                                  >  1:659269/1‑150 (MQ=255)
                                  tAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCaa                                                                                                                 >  2:770790/1‑150 (MQ=255)
                                    aaTGGTCATAATGCACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGATCAAGTGACGGGGCAGTGCAATATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCaaaa                                                                                                               <  1:921587/150‑1 (MQ=255)
                                          cATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAATATTGTGATGTAAAGCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAAc                                                                                                         <  2:703348/150‑1 (MQ=255)
                                           aTAATTAACAACACCAGAAAGATAAAAATTTAAAATAAATACAGAAATACTTTAGAGCAAGGGACGGGGAAGTGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAAcc                                                                                                        <  1:579921/150‑1 (MQ=255)
                                                gAACAACCCCAGAAAGCGAAAAATATAAAATACAAACAGAAAGACATGAGAGCAAGCGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTgg                                                                                                   <  2:119005/150‑1 (MQ=255)
                                                  acaacaCCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGaa                                                                                                 >  1:684713/1‑150 (MQ=255)
                                                     acacCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACATTTGGAATaa                                                                                              >  2:236889/1‑150 (MQ=255)
                                                      cacCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAAc                                                                                             <  2:511451/150‑1 (MQ=255)
                                                             aaGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAg                                                                                      >  2:706050/1‑150 (MQ=255)
                                                               gagaAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGAt                                                                                    <  2:692223/150‑1 (MQ=255)
                                                                      ttataAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATAATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTAAACCTTTGGAATAACTTAAAATATAAAAAAg                                                                             >  1:40063/2‑150 (MQ=255)
                                                                      atatAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATAAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAg                                                                             >  2:1208606/1‑150 (MQ=255)
                                                                          aaaaTACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACg                                                                         >  1:554959/1‑150 (MQ=255)
                                                                                catacaGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGt                                                                   >  2:715937/1‑150 (MQ=255)
                                                                                     aGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTa                                                              >  2:170807/1‑150 (MQ=255)
                                                                                      gAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGCCGCAAGGTGAGTAt                                                             >  1:1041345/1‑150 (MQ=255)
                                                                                         aGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTACCGCCTTCAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCATGTGAGTATTCa                                                          >  2:58102/1‑150 (MQ=255)
                                                                                         aGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTACCGCCTACAGGTGATCAAAATTTAACCTTTCGAATAACTAAAAAGAGAATAAAGGACGCCAGGTGAGTATTCa                                                          >  1:628135/1‑150 (MQ=255)
                                                                                            cATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAg                                                       <  1:39901/150‑1 (MQ=255)
                                                                                            cATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTAATCTTTGATTTAACGCCTACAGTTGATCAAAATTTAACCTTTGGATTAACTAAAAAGATAAAAAAGGACGCAAGGTGAGTATTCAAAg                                                       >  1:984113/1‑150 (MQ=255)
                                                                                                  agCAAGGGACGGGTCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCt                                                 <  2:820667/150‑1 (MQ=255)
                                                                                                     aaGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGa                                              >  1:3465/1‑150 (MQ=255)
                                                                                                         gACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATtat                                          >  2:1205552/1‑150 (MQ=255)
                                                                                                         gACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATtat                                          >  1:263771/1‑150 (MQ=255)
                                                                                                           cGGGGCAGGGCAAGTTAATTAAAATTGTGATGTAAATAACGATTTTCATATTTGCTTTAACGCCTACAGGTGATCAAAATTTAAACTTTGGAATAACTAAAAATATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTatat                                        <  2:1017271/150‑1 (MQ=255)
                                                                                                           cGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTatat                                        <  2:1012045/150‑1 (MQ=255)
                                                                                                              ggcagggcaAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTc                                     >  1:51116/1‑150 (MQ=255)
                                                                                                               gcagggcaAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCa                                    <  1:228692/150‑1 (MQ=255)
                                                                                                               gcagggcaAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCa                                    <  1:82145/150‑1 (MQ=255)
                                                                                                                   ggcaAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGa                                >  2:471412/1‑150 (MQ=255)
                                                                                                                       caaagaTGAAAATCGTGATTTACATCACAATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGAcccc                            >  1:471553/6‑150 (MQ=39)
                                                                                                                           cATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGTTGATAAAAATTTAAAATTTGTAATAAATAAAAAGATAAAAAATGAAGAAATTTAAGTATTCAAAGTTTGCTTGATTATATTTCATTGAACACTGat                        >  2:107934/1‑150 (MQ=255)
                                                                                                                            aTGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGata                       >  1:588774/1‑150 (MQ=255)
                                                                                                                            aTGAAAATCGTGATTTACATCACAATTTTCTTCTTTGGTTTACCGCCTACAGGTGATCATATTTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGGGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGata                       >  1:864673/1‑150 (MQ=255)
                                                                                                                             tGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGatat                      <  2:588774/150‑1 (MQ=255)
                                                                                                                                       gATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACGAcaacaa            >  2:195175/1‑150 (MQ=255)
                                                                                                                                          gTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACGACAACAAGGa         >  1:1238648/1‑150 (MQ=255)
                                                                                                                                            aaaTCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACGTCAACAAGGaaa       >  1:1077792/1‑150 (MQ=255)
                                                                                                                                             aaTCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACGACAACAAGGAAAg      <  1:69161/150‑1 (MQ=255)
                                                                                                                                                cACGATTTTCATATTTGCTTTAACGCCTACAGGTTATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACGACAACAAGGAAAGGtt   <  1:949611/150‑1 (MQ=255)
                                                                                                                                                 aCGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGCAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCATTGACACCTGATATACGACAACAAGGAAAGGTTa  >  2:563752/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
ACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATGAAAATTGTGATGTAAATCACGATTTTCATCTTTGCTTTAACGCCTACAGGTGATCAAAATTTAACCTTTGGAATAACTAAAAAGATAAAAAAGGACGCCAGGTGAGTATTCAAAGTTTGCTTGATTATATTTCAGTGACCCCTGATATACGACAACAAGGAAAGGTTA  >  NC_000913/1530358‑1530652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑