Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,579,563 G→T 10.7% intergenic (‑221/+70) yddA ← / ← ydeM putative multidrug ABC transporter permease/ATPase/putative YdeN‑specific sulfatase‑maturating enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,579,5630GT10.7% 159.1 / 7.6 71intergenic (‑221/+70)yddA/ydeMputative multidrug ABC transporter permease/ATPase/putative YdeN‑specific sulfatase‑maturating enzyme
Reads supporting (aligned to +/- strand):  ref base G (25/38);  new base T (3/5);  total (28/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

ATTTAACAATATGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCTTAGCTAACTCTACCATGG  >  NC_000913/1579415‑1579709
                                                                                                                                                    |                                                                                                                                                  
aTTTAACAATATGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAgg                                                                                                                                                   <  1:379291/150‑1 (MQ=255)
aTTTAACAATATGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAgg                                                                                                                                                   >  2:549572/1‑150 (MQ=255)
   tAACAATATGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGTATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAAt                                                                                                                                                <  1:262057/150‑1 (MQ=255)
      aaaTATGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTa                                                                                                                                             <  2:290517/149‑1 (MQ=255)
        atatGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATg                                                                                                                                           >  2:1244712/1‑150 (MQ=255)
          atGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTc                                                                                                                                         <  2:336520/150‑1 (MQ=255)
             aaaTATAAAGGAATATTTCATTTTCAGTGTGTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACAACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAACCACACGACAGGAATATATGTAggg                                                                                                                                      >  1:1064945/1‑150 (MQ=255)
             aaaTATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACATAAATTCCTGTCggg                                                                                                                                      <  1:318455/150‑1 (MQ=255)
             aaaTATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCAGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACATAAATTCCTGTCggg                                                                                                                                      <  1:1166323/150‑1 (MQ=255)
              aaTATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACATAAATTCCTGTCGGGt                                                                                                                                     <  1:42587/150‑1 (MQ=255)
              aaTATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATCACGAAACACCCGACAGGAATTTATGTCGGGt                                                                                                                                     >  1:308319/1‑150 (MQ=255)
               atatatAGGAATATTTCATTTTCAGTGGATATGGATATGCTATTGATAATCAAGGATATAGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTg                                                                                                                                    <  2:859764/150‑1 (MQ=255)
                  taAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCt                                                                                                                                 >  1:1095597/1‑150 (MQ=255)
                   aaaGGAATATTTCATTATCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCtt                                                                                                                                <  2:540767/150‑1 (MQ=255)
                    aaGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATCATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGAGGATGACGAAACACCCGACATAAATTCCTGTCGGGTgtttcg                                                                                                                               <  1:838965/150‑6 (MQ=255)
                    aaGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTg                                                                                                                               <  1:161869/150‑1 (MQ=255)
                    aaGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTg                                                                                                                               <  2:813537/150‑1 (MQ=255)
                         atatTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAg                                                                                                                          <  2:1033167/150‑1 (MQ=255)
                          tatTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAgg                                                                                                                         <  2:707134/150‑1 (MQ=255)
                             ttCATTTTCAGTGGTTATGGATATGCTATTGATAATCAAGGATATTGCTAATGCCCAGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGATGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCa                                                                                                                      <  1:1212602/150‑1 (MQ=255)
                               cATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATTACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATa                                                                                                                    >  2:84801/1‑150 (MQ=255)
                                     cAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAagg                                                                                                              <  1:1297316/150‑1 (MQ=255)
                                             atggttatgCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAg                                                                                                      <  1:700137/150‑1 (MQ=255)
                                              tggttatgCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGa                                                                                                     >  1:1288105/1‑150 (MQ=255)
                                                   atgCTATTGATAATCAAGGATATTGCGAATGCCCGGATGCATCCCACGTAAGCCAGGATTTTCGCAAGGGAAGATGAGGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGa                                                                                                <  1:670842/150‑1 (MQ=255)
                                                    tgCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATCGat                                                                                               >  2:1252464/1‑149 (MQ=255)
                                                     gCTATTGATAATCAAGGAAATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGt                                                                                              <  1:70430/150‑1 (MQ=255)
                                                            aTAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAaga                                                                                       >  1:698175/1‑150 (MQ=255)
                                                               aTCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAagagag                                                                                    >  2:465235/1‑150 (MQ=255)
                                                                   aGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCggt                                                                                >  2:630088/1‑148 (MQ=255)
                                                                    ggATATTGCTAATGCCCTGATGCATACCCCGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCCTAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGttt                                                                               >  1:1070975/1‑149 (MQ=255)
                                                                      atatTGCTAATGCAATTATGCATACCACGTAAGCCAGGATTTTCGCAAGTGAAGATGATGATTACGAAACACCCGACATAAATTCCTGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTTAGAATGGAGTTAAAAGAGAGCGttatt                                                                             <  1:821711/150‑1 (MQ=255)
                                                                       tatTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGttatta                                                                            <  1:995648/150‑1 (MQ=255)
                                                                         ttGCTAATGCCCATTTGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGCTGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTattaat                                                                          <  2:221281/150‑1 (MQ=255)
                                                                          tGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTattaatt                                                                         <  1:436951/150‑1 (MQ=255)
                                                                           gCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTattaatta                                                                        >  2:529343/1‑150 (MQ=255)
                                                                           gCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGCAGGCTAAGAATGGAGTTAAAAGAGAGCGTTattaatta                                                                        >  1:688887/1‑150 (MQ=255)
                                                                           gCTAATGCCATGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTattaatta                                                                        <  2:135887/150‑1 (MQ=255)
                                                                              aaTGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATtc                                                                     <  2:42440/150‑1 (MQ=255)
                                                                              aaTGCCCTGATGCATACCACGTAAGCCAGGATTTACGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATTTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATGTattatta                                                                     >  1:803975/1‑149 (MQ=255)
                                                                               aTGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAACAGAGCGTTAATAATTATtca                                                                    >  2:130756/1‑149 (MQ=255)
                                                                                  cccTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGAGGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCa                                                                 >  1:161589/1‑150 (MQ=255)
                                                                                   ccTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCAt                                                                >  1:25569/1‑150 (MQ=255)
                                                                                   ccTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCAt                                                                <  2:1274854/150‑1 (MQ=255)
                                                                                      gATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTg                                                             <  1:157123/150‑1 (MQ=255)
                                                                                      gATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTg                                                             <  1:1257320/150‑1 (MQ=255)
                                                                                        tGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGTCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGtt                                                           >  1:101556/1‑150 (MQ=255)
                                                                                        tGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGtt                                                           >  1:285455/1‑150 (MQ=255)
                                                                                          cATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGtttt                                                         <  1:582849/150‑1 (MQ=255)
                                                                                              ccACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCaa                                                     <  2:698175/150‑1 (MQ=255)
                                                                                                  gTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATc                                                 <  2:335873/150‑1 (MQ=255)
                                                                                                          ggATTTTCGCATGGTAAGATGATGATGACGAAACACCCGACAGTAATTTATGTCGTGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGTATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAAtgt                                         <  1:660621/150‑1 (MQ=255)
                                                                                                          ggATTTTCGCAAGGGAAGATGATGTTGACGAAACAACCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAAtgt                                         <  1:281480/150‑1 (MQ=255)
                                                                                                           gATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAAtgtg                                        >  1:836993/1‑150 (MQ=255)
                                                                                                            aTTTTCGCAAGGGAAGATGAGGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTgg                                       <  2:1211936/150‑1 (MQ=255)
                                                                                                                  gCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGc                                 <  2:25569/150‑1 (MQ=255)
                                                                                                                   cAAGGGAAGATGATGATGACGAAACACCCGACAGGATTTTATGTCGGGTGCCTTGTTAAGGTCATGAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCg                                >  2:341486/1‑150 (MQ=255)
                                                                                                                   cAAGGGAAGAAGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGATAAAAGAGAGCGTTATTAATTATACTCCAATTGTTTTGCAAAATCCATAATGTGGTAAAGCg                                >  2:894482/1‑150 (MQ=255)
                                                                                                                   cAACGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCg                                <  2:629409/150‑1 (MQ=255)
                                                                                                                     aGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGt                              >  2:615475/1‑150 (MQ=255)
                                                                                                                       ggAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTAc                            >  2:1095442/1‑150 (MQ=255)
                                                                                                                           gatgatgatGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTg                        <  1:1124940/150‑1 (MQ=255)
                                                                                                                                tgatgaCGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCtt                   <  1:269231/150‑1 (MQ=255)
                                                                                                                                        aaaCACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGCGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCTTAGCTAAct           >  1:503470/1‑150 (MQ=255)
                                                                                                                                          gcacCCGACATAAATTCCTGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTAAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCTTAGCTAActct         >  2:269411/2‑150 (MQ=255)
                                                                                                                                            acCCGACATAAATTCCTGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCTTAGCTAACTCTAc       >  1:1186342/1‑150 (MQ=255)
                                                                                                                                             cccGACATAAATTCCTGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCAGAATGTGGTAAAGCGGTACTCTGTTCTTAGCTAACTCTAcc      >  2:887645/1‑150 (MQ=255)
                                                                                                                                             cccGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAATAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCTGTACTCTGTTCTTAGCTAACTCTAcc      <  2:686932/150‑1 (MQ=255)
                                                                                                                                               cGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTTATAAGAAGGAGTCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCTTAGCTAACTCTACCAt    <  2:101556/150‑1 (MQ=255)
                                                                                                                                               cGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCTTAGCTAACTCTACCAt    <  2:285455/150‑1 (MQ=255)
                                                                                                                                                gACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCTTAGCTAACTCTACCATg   <  2:260693/150‑1 (MQ=255)
                                                                                                                                                 ccAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCTTAGCTAACTCTACCATgg  <  2:461686/149‑1 (MQ=255)
                                                                                                                                                 aCAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCTTAGCTAACTCTACCATgg  <  2:1228427/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
ATTTAACAATATGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCTTAGCTAACTCTACCATGG  >  NC_000913/1579415‑1579709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: