Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,579,569 T→C 11.3% intergenic (‑227/+64) yddA ← / ← ydeM putative multidrug ABC transporter permease/ATPase/putative YdeN‑specific sulfatase‑maturating enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,579,5690TC11.3% 188.3 / 14.6 71intergenic (‑227/+64)yddA/ydeMputative multidrug ABC transporter permease/ATPase/putative YdeN‑specific sulfatase‑maturating enzyme
Reads supporting (aligned to +/- strand):  ref base T (25/38);  new base C (3/5);  total (28/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.49e-01

CAATATGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCTTAGCTAACTCTACCATGGCGTTCAT  >  NC_000913/1579421‑1579716
                                                                                                                                                    |                                                                                                                                                   
aaaTATGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTa                                                                                                                                                    <  2:290517/149‑1 (MQ=255)
  atatGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATg                                                                                                                                                  >  2:1244712/1‑150 (MQ=255)
    atGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTc                                                                                                                                                <  2:336520/150‑1 (MQ=255)
       aaaTATAAAGGAATATTTCATTTTCAGTGTGTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACAACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAACCACACGACAGGAATATATGTAggg                                                                                                                                             >  1:1064945/1‑150 (MQ=255)
       aaaTATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACATAAATTCCTGTCggg                                                                                                                                             <  1:318455/150‑1 (MQ=255)
       aaaTATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCAGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACATAAATTCCTGTCggg                                                                                                                                             <  1:1166323/150‑1 (MQ=255)
        aaTATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACATAAATTCCTGTCGGGt                                                                                                                                            <  1:42587/150‑1 (MQ=255)
        aaTATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATCACGAAACACCCGACAGGAATTTATGTCGGGt                                                                                                                                            >  1:308319/1‑150 (MQ=255)
         atatatAGGAATATTTCATTTTCAGTGGATATGGATATGCTATTGATAATCAAGGATATAGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTg                                                                                                                                           <  2:859764/150‑1 (MQ=255)
            taAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCt                                                                                                                                        >  1:1095597/1‑150 (MQ=255)
             aaaGGAATATTTCATTATCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCtt                                                                                                                                       <  2:540767/150‑1 (MQ=255)
              aaGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATCATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGAGGATGACGAAACACCCGACATAAATTCCTGTCGGGTgtttcg                                                                                                                                      <  1:838965/150‑6 (MQ=255)
              aaGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTg                                                                                                                                      <  2:813537/150‑1 (MQ=255)
              aaGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTg                                                                                                                                      <  1:161869/150‑1 (MQ=255)
                   atatTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAg                                                                                                                                 <  2:1033167/150‑1 (MQ=255)
                    tatTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAgg                                                                                                                                <  2:707134/150‑1 (MQ=255)
                       ttCATTTTCAGTGGTTATGGATATGCTATTGATAATCAAGGATATTGCTAATGCCCAGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGATGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCa                                                                                                                             <  1:1212602/150‑1 (MQ=255)
                         cATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATTACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATa                                                                                                                           >  2:84801/1‑150 (MQ=255)
                               cAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAagg                                                                                                                     <  1:1297316/150‑1 (MQ=255)
                                       atggttatgCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAg                                                                                                             <  1:700137/150‑1 (MQ=255)
                                        tggttatgCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGa                                                                                                            >  1:1288105/1‑150 (MQ=255)
                                             atgCTATTGATAATCAAGGATATTGCGAATGCCCGGATGCATCCCACGTAAGCCAGGATTTTCGCAAGGGAAGATGAGGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGa                                                                                                       <  1:670842/150‑1 (MQ=255)
                                              tgCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATCGat                                                                                                      >  2:1252464/1‑149 (MQ=255)
                                               gCTATTGATAATCAAGGAAATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGt                                                                                                     <  1:70430/150‑1 (MQ=255)
                                                      aTAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAaga                                                                                              >  1:698175/1‑150 (MQ=255)
                                                         aTCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAagagag                                                                                           >  2:465235/1‑150 (MQ=255)
                                                             aGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCggt                                                                                       >  2:630088/1‑148 (MQ=255)
                                                              ggATATTGCTAATGCCCTGATGCATACCCCGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCCTAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGttt                                                                                      >  1:1070975/1‑149 (MQ=255)
                                                                atatTGCTAATGCAATTATGCATACCACGTAAGCCAGGATTTTCGCAAGTGAAGATGATGATTACGAAACACCCGACATAAATTCCTGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTTAGAATGGAGTTAAAAGAGAGCGttatt                                                                                    <  1:821711/150‑1 (MQ=255)
                                                                 tatTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGttatta                                                                                   <  1:995648/150‑1 (MQ=255)
                                                                   ttGCTAATGCCCATTTGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGCTGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTattaat                                                                                 <  2:221281/150‑1 (MQ=255)
                                                                    tGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTattaatt                                                                                <  1:436951/150‑1 (MQ=255)
                                                                     gCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTattaatta                                                                               >  2:529343/1‑150 (MQ=255)
                                                                     gCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGCAGGCTAAGAATGGAGTTAAAAGAGAGCGTTattaatta                                                                               >  1:688887/1‑150 (MQ=255)
                                                                     gCTAATGCCATGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTattaatta                                                                               <  2:135887/150‑1 (MQ=255)
                                                                        aaTGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATtc                                                                            <  2:42440/150‑1 (MQ=255)
                                                                        aaTGCCCTGATGCATACCACGTAAGCCAGGATTTACGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATTTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATGTattatta                                                                            >  1:803975/1‑149 (MQ=255)
                                                                         aTGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAACAGAGCGTTAATAATTATtca                                                                           >  2:130756/1‑149 (MQ=255)
                                                                            cccTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGAGGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCa                                                                        >  1:161589/1‑150 (MQ=255)
                                                                             ccTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCAt                                                                       >  1:25569/1‑150 (MQ=255)
                                                                             ccTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCAt                                                                       <  2:1274854/150‑1 (MQ=255)
                                                                                gATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTg                                                                    <  1:1257320/150‑1 (MQ=255)
                                                                                gATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTg                                                                    <  1:157123/150‑1 (MQ=255)
                                                                                  tGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGTCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGtt                                                                  >  1:101556/1‑150 (MQ=255)
                                                                                  tGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGtt                                                                  >  1:285455/1‑150 (MQ=255)
                                                                                    cATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGtttt                                                                <  1:582849/150‑1 (MQ=255)
                                                                                        ccACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCaa                                                            <  2:698175/150‑1 (MQ=255)
                                                                                            gTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATc                                                        <  2:335873/150‑1 (MQ=255)
                                                                                                    ggATTTTCGCATGGTAAGATGATGATGACGAAACACCCGACAGTAATTTATGTCGTGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGTATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAAtgt                                                <  1:660621/150‑1 (MQ=255)
                                                                                                    ggATTTTCGCAAGGGAAGATGATGTTGACGAAACAACCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAAtgt                                                <  1:281480/150‑1 (MQ=255)
                                                                                                     gATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAAtgtg                                               >  1:836993/1‑150 (MQ=255)
                                                                                                      aTTTTCGCAAGGGAAGATGAGGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTgg                                              <  2:1211936/150‑1 (MQ=255)
                                                                                                            gCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGc                                        <  2:25569/150‑1 (MQ=255)
                                                                                                             cAAGGGAAGATGATGATGACGAAACACCCGACAGGATTTTATGTCGGGTGCCTTGTTAAGGTCATGAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCg                                       >  2:341486/1‑150 (MQ=255)
                                                                                                             cAAGGGAAGAAGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGATAAAAGAGAGCGTTATTAATTATACTCCAATTGTTTTGCAAAATCCATAATGTGGTAAAGCg                                       >  2:894482/1‑150 (MQ=255)
                                                                                                             cAACGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCg                                       <  2:629409/150‑1 (MQ=255)
                                                                                                               aGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGt                                     >  2:615475/1‑150 (MQ=255)
                                                                                                                 ggAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTAc                                   >  2:1095442/1‑150 (MQ=255)
                                                                                                                     gatgatgatGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTg                               <  1:1124940/150‑1 (MQ=255)
                                                                                                                          tgatgaCGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCtt                          <  1:269231/150‑1 (MQ=255)
                                                                                                                                  aaaCACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGCGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCTTAGCTAAct                  >  1:503470/1‑150 (MQ=255)
                                                                                                                                    gcacCCGACATAAATTCCTGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTAAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCTTAGCTAActct                >  2:269411/2‑150 (MQ=255)
                                                                                                                                      acCCGACATAAATTCCTGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCTTAGCTAACTCTAc              >  1:1186342/1‑150 (MQ=255)
                                                                                                                                       cccGACATAAATTCCTGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCAGAATGTGGTAAAGCGGTACTCTGTTCTTAGCTAACTCTAcc             >  2:887645/1‑150 (MQ=255)
                                                                                                                                       cccGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAATAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCTGTACTCTGTTCTTAGCTAACTCTAcc             <  2:686932/150‑1 (MQ=255)
                                                                                                                                         cGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTTATAAGAAGGAGTCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCTTAGCTAACTCTACCAt           <  2:101556/150‑1 (MQ=255)
                                                                                                                                         cGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCTTAGCTAACTCTACCAt           <  2:285455/150‑1 (MQ=255)
                                                                                                                                          gACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCTTAGCTAACTCTACCATg          <  2:260693/150‑1 (MQ=255)
                                                                                                                                           ccAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCTTAGCTAACTCTACCATgg         <  2:461686/149‑1 (MQ=255)
                                                                                                                                           aCAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCTTAGCTAACTCTACCATgg         <  2:1228427/150‑1 (MQ=255)
                                                                                                                                                 aTTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCTTAGCTAACTCTACCATGGCGTTCa   <  1:699598/150‑1 (MQ=255)
                                                                                                                                                  tttATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCTTAGCTAACTCTACCATGGCGTTCat  <  1:1139985/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CAATATGAAATATAAAGGAATATTTCATTTTCAGTGGTTATGGTTATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCCATAATGTGGTAAAGCGGTACTCTGTTCTTAGCTAACTCTACCATGGCGTTCAT  >  NC_000913/1579421‑1579716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: