Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,696,187 A→G 7.0% intergenic (‑116/+275) uidA ← / ← uidR beta‑D‑glucuronidase/transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,696,1870AG 7.0% 208.8 / 5.8 71intergenic (‑116/+275)uidA/uidRbeta‑D‑glucuronidase/transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base A (47/19);  new base G (2/3);  total (49/22)
Fisher's exact test for biased strand distribution p-value = 1.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01

TCACGGGTTGGGGTTTCTACAGGACGTAACATAAGGGACTCCTCATTAAGATAATAATACTGGTCAACCTTTAATCTGATTAGTTAACCAATTCTGTGTTGCACACAGTTATCTGTGAGAAACCCATGGAAGAGAAATGGAAATAAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCATTAATCTTTAAAACAGCGTTGACAGAAGCCATAAAATAAGCAACCGAACGTTCGTTCGAGCGTATTGGGAACGATAAACTCTCATG  >  NC_000913/1696040‑1696335
                                                                                                                                                   |                                                                                                                                                    
tCACGGGTTGGGGTTTCTACAGGACGTAACATAAGGGACTCCTCATTAAGATAATAATACTGGTCAACCTTTAATCTGATTAGTTAACCAATTCTGTGTTGCACACAGTTATCTGTGAGAAACCCATGGAAGAGAAATGGAAATAAAAAg                                                                                                                                                    >  1:324423/1‑150 (MQ=255)
 cACGGGTTGGGGTTTCTACAGGACGTAACATAAGGGACTCCTCATTAAGATAATAATACTGGTCAACCTTTAATCTGATTAGTTAACCAATTCTGTGTTGCACCCAGTTATCAGTGAGCAACCCATGGAAGAGAAATGGAAATAAAAAGa                                                                                                                                                   >  2:248463/1‑150 (MQ=255)
       ttGGGGTTTCTACAGGACGTAACATAAGGGACTCCTCATTAAGATAATAATACTGGTCAACCTTTAATCTGATTAGTTAACCAATTCTGTGTTGCACACAGTTATCTGTGAGAAACCCATGGAAGAGAAATGGAAATAAAAAGAACTGac                                                                                                                                             <  1:1298995/150‑1 (MQ=255)
         ggggTTTCTACAGGACGTAACATAAGGGACTCCTCATTAAGATAATAATACTGGTCAACCTTTAATCTGATTAGTTAACCAATTCTGTGTTGCACACAGTTATCTGTGAGAAACCCATGGAAGAGAAATGGAAATAAAAAGAACTGacac                                                                                                                                           >  2:607610/1‑150 (MQ=255)
         ggggTTTCTACAGGACGTAACATAAGGGACTCCTCATTAAGATAATAATACTGGTCAACCTTTAATCTGATTAGTTAACCAATTCTGTGTTGCACACAGTTATCTGTGAGAAACCCATGGAAGAGAAATGGAAATAAAAAGAACTGacac                                                                                                                                           >  2:1114169/1‑150 (MQ=255)
           ggTTTCTACAGGACGTAACATAAGGGACTCCTCATTAAGATAATAATACTGGTCAACCTTTAATCTGATTAGTTAACCAATTCTGTGTTGCACACAGTTATCTGTGAGAAACCCATGGAAGAGAAATGGAAATAAAAAGAACTGACACcg                                                                                                                                         >  2:635352/1‑150 (MQ=255)
             tttCTACAGGACGTAACATAAGGGACTCCTCATTAAGATAATAATACTGGTCAACCTTTAATCTGATTAGTTAACCAATTCTGTGTTGCACACAGTTATCTGTGAGAAACCCATGGAAGAGAAATGGAAATAAAAAGAACTGACACcgcg                                                                                                                                       <  1:1001118/150‑1 (MQ=255)
                  aCAGGACGTAACATAAGGGACTCCTCATTAAGATAATAATACTGGTCAACCTTTAATCTGATTAGTTAACCAATTCTGTGTTGCACACAGTTATCTGTGAGAAACCCATGGAAGAGAAATGGAAATAAAGAGAACTGCCACCGCGATCaa                                                                                                                                  >  1:1141272/1‑150 (MQ=255)
                  aCAGGACGTAACATAAGGGACTCCTCATTAAGATAATAATACTGGTCAACCTTTAATCTGATTAGTTAACCAATTCTGTGTTGCACACAGTTATCTGTGAGAAACCCATGGAAGAGAAATGGAAATAAAGAGAACTGACACCGCGATCaa                                                                                                                                  >  1:1136196/1‑150 (MQ=255)
                     ggACGTAACATAAGGGACTCCTCATTAAGATAATAATAATGGTCAACCTTTAATCTCATTAGTTAACCAAATATGTGTTGAACACAGTTATATGACAGAAACCAATGGAAAAAAAATCAAAATAAAAAGAACTGAAAACTCTATaaaaaa                                                                                                                               >  1:756122/1‑150 (MQ=255)
                      gACGTAACATAAGGGACTCCTCATTAAGATAATAATACTGGTCAACCTTTAATCTGATTAGTTAACCAATTCTGTGTTGCACACAGTTATCTGTGAGAAACCCATGGAAGAGAAATTGAAATAAAAAGAAATGACACCGCGATCaaaaaa                                                                                                                              >  1:119668/1‑150 (MQ=255)
                         gTAACATAAGGGACTCCTCATTAAGATAATAATACTGGTCAACCTTTAATCTGATTAGTTAACCAATTCTGTGTTGCACACAGTTATCTGTGAGAAACCCATGGAAGAGAAATGGAAATAAAAAGAACTGACACCGCGATCAAAAAACCa                                                                                                                           >  1:362166/1‑150 (MQ=255)
                             cATAAGGGACTCCTCATTAAGATAATAATACTGGTCAACCTTTAATCTGATTAGTTAACCAATTCTGTGTTGCACACAGTTATCTGTGAGAAACCCATGGAAGAGAAATGGAAATAAAAAGAACTGACACCGCGATCAAAAAACCaaaaa                                                                                                                       >  1:471022/1‑150 (MQ=255)
                             cATAAGGGACTCCTCATTAAGATAATAATACTGGTCAACCTTTAATCTGATTAGTTAACCAATTCTGTGTTGCACACAGTTATCTGTGAGAAACCCATGGAAGAGAAATGGAAATAAAAAGAACTGACACCGCGATCAAAAAACCaaaaa                                                                                                                       >  2:1013120/1‑150 (MQ=255)
                                aaGGGACTCCTCATTAAGATAATAATACTGGTCAACCTTTAATCTGATTAGTTAACCAATTCTGTGTTGCACACAGTTATCTGTGAGAAACCCATGGAAGAGAAATGGAAATAAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTg                                                                                                                    >  1:1042968/1‑150 (MQ=255)
                                      ctcctcATAAAGATAATAATACTGGTCAACCTTTAATCTGATTAGTTAACCAATTCTGTGTTGCACACAGTTATCTGTGAGAAACCCATGGAAGAGAAATGGAAATAAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATAc                                                                                                              <  2:194782/150‑1 (MQ=255)
                                         ctcATTAAGATAATAATACTGGTCAACCTTTAATCTGATTAGTTAACCAATTCTGTGTTGCACACAGTTATCTGTGAGAAACCCATGGAAGAGAAATGGAAATAAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGAAATACCca                                                                                                           >  1:350830/1‑150 (MQ=255)
                                           cATTAAGATAATAATACTGGTCAACCTTTAATCTGATTAGTTAACCAATTCTGTGTTGCACACAGTTATCTGTGAGCAACCCATGGAAGAGAAATGGAAATAAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCcaca                                                                                                         >  2:390358/1‑150 (MQ=255)
                                           cATTAAGATAATAATACTGGTCAACCTTTAATCTGATTAGTTAACCAATTCTGTGTTGCACACAGTTATCTGTGAGAACCCCATGGAAGAGAAATGGAAATAAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCcaca                                                                                                         >  2:940441/1‑150 (MQ=255)
                                                        ataCTGGTCAACCTTTAATCTGATTAGTTAACCAATTCTGTGTTGCACACAGTTATCTGTGAGAAACCCATGGAAGAGAAATGGAAATAAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGAt                                                                                            >  2:748745/1‑150 (MQ=255)
                                                             ggTCAACCTTTAATCTGATTAGTTAACCAATTCTGTGTTGCACACAGTTATCTGTGAGAAACCCATGGAAGAGAAATTGAAATAAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCAt                                                                                       >  1:256924/1‑150 (MQ=255)
                                                               tCAACCTTTAATCTGATTAGTTAACCAATTCTGTGTTGCACACAGTTATCTGTGAGAAACCCATGGAAGAGAAATGGAAATAAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCATTa                                                                                     >  2:317242/1‑150 (MQ=255)
                                                                  aCCTTTAATCTGATTAGTTAACCAATTCTGTGTTGCACACAGTTATCTGTGAGAAACCCATGGAAGAGAAATGGAAATAAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCATTAATc                                                                                  <  1:859771/150‑1 (MQ=255)
                                                                  aCCTTTAATCTGATTAGTTAACCAATTCTGTGTTGCACACAGTTATCTGTGAGAAACCCATGGAAGAGAAATGGAAATAAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCATTAATc                                                                                  >  1:62228/1‑150 (MQ=255)
                                                                     tttAATCTTATTAGTTAACCAATTCTGTGTTGCACACAGTTATCTGTGAGAAACCCATGGAAGAGAAATGGAAATAAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCATTAATCttt                                                                               <  1:57553/150‑1 (MQ=255)
                                                                     tttAATCTGATTAGTTAACCAATTCTGTGTTGCACACAGTTATCTGTGAGAAACCCATTGAAGAGAAATGTAAATAAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCATTAATCttt                                                                               <  1:1162921/150‑1 (MQ=255)
                                                                        aaTCTGATTAGTTAACCAATTCTGTGTTGCACACAGTTATCTGTGAGAAACCCATGGAAGAGAAATGGAAATAAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCATTAATCTTTaaa                                                                            <  2:50370/150‑1 (MQ=255)
                                                                             gATTAGTTAACCAATTCTGTGTTGCACACAGTTATCTGTGAGAAACCCATGGAAGAGAAATGGAAATAAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCATTAATCTTTAAAACAGc                                                                       >  2:1194005/1‑150 (MQ=255)
                                                                             gATTAGTTAACCAATTCTGTGTTGCACACAGTTATCTGTGAGAAACCCATGGAAGAGAAATGGAAATAAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCATTAAGCTTTAAAACAGc                                                                       >  2:1106769/1‑150 (MQ=255)
                                                                               ttAGTTAACCAATTCTGTGTTGCACACAGTTATCTGTGAGAAACCCATGGAAGAGAAATGGAAATAAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCATTAATCTTTAAAACAGCGt                                                                     <  1:635352/150‑1 (MQ=255)
                                                                               ttAGTTAACCAATTCTGTGTTGCACACAGTTATCTGTGAGAAACCCATGGAAGAGAAATGGAAATAAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCATTAATCTTTAAAACAGCGt                                                                     >  2:455596/1‑150 (MQ=255)
                                                                                tAGTTAACCAATTCTGTGTTGCACACAGTTATCTGTGAGAAACCCATGGAAGAGAAATGGAAATAAAGAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCATTAATCTTTAAAACAGCGtt                                                                    <  1:1095494/150‑1 (MQ=255)
                                                                                 aGTTAACCAATTCTGTGTTGCACACAGTTATCTGTGAGAAACCCATGGAAGAGAAATGGAAATAAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCATTAATCTTTAAAACAGCGTTg                                                                   >  2:606096/1‑150 (MQ=255)
                                                                                 aGTTAACCAATTCTGTGTTGCACACAGTTATCTGTGAGAAACCCATGGAAGAGAAATGGAAATAAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCATTAATCTTTAAAACAGCGTTg                                                                   >  2:1248732/1‑150 (MQ=255)
                                                                                 aGTTAACCAATTCTGTGTTGCACACAGTTATCTGTGAGAAACCCATGGAAGAGAAATGGAAATAAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCATTAATCTTTAAAACAGCGTTg                                                                   <  2:1223328/150‑1 (MQ=255)
                                                                                 aGTTAACCAATTCTGTGTTGCACAAAGTTATCTGTGAGAAACCCATGGATGAGAAATGGAAATTAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGAGAGATATAGATCCCATTAATCTTTAAAAAAGAGttt                                                                   >  1:799277/1‑149 (MQ=255)
                                                                                  gTTAACCAATTCTGTGTTGCACACTGTTATCTGTGAGAAACCCATGGAAGAGAAATGGAAATAAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCATTAATCTTTAAAACAGCGTTGa                                                                  >  2:522211/1‑150 (MQ=255)
                                                                                   ttAACCAATTCTGTGTTGCACACAGTTATCTGTGAGAAACCCATGGAAGAGAAATGGAAATAAAAAGATCTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCATTAATCTTTAAAACAGCGTTGAc                                                                 >  1:582929/1‑150 (MQ=255)
                                                                                    tAACCAATTCTGTGTTGCACACAGTTATCTGTGAGAAACCCATTGAACAGAAATGGAAATAAAAAGAACTGACAACGCCATCAAAAAACCAAAAACAGCAATAACCACTGCGTGATATAGTTCGCATTTATCTTTAAACCAGCGTTGCCa                                                                >  1:107179/1‑150 (MQ=255)
                                                                                      aCCAATTCTGTGTTGCACACAGTTATCTGTGAGAAACCCATGGAAGAGAAATGGAAATAAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCATTAATCTTTAAAACAGCGTTGACAGa                                                              >  2:357454/1‑150 (MQ=255)
                                                                                      aCCAATTATGTGTTGCACACAGTTATCTGTGAGAAACCCATGGAAGAGAAATTGAAATAAAAAGACCTGACACCGCGAGCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCATTAATCTTTAAAACAGCGTTGACAGa                                                              <  1:810664/150‑1 (MQ=255)
                                                                                        cAATTCTGTGTTGCACACAGTTATCTGTGAGAAACCCATGGAAGAGAAATGGAAATAAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGCTCGCATTAATCTTTAAAACAGCGTTGACAGAAg                                                            >  1:1111448/1‑150 (MQ=255)
                                                                                        cAATTCTGTGTTGCACACAGTTATCTGTGAGAAACCCATGGAAGAGAAATGGAAATAAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCATTAATCTTTAAAACAGCGATGACAGAAg                                                            >  1:418325/1‑150 (MQ=255)
                                                                                           ttCTGTGTTGCACACAGTTATCTGTGAGAAACCCATGGAAGAGAAATGGAAATAAAAAAAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCATTAATCTTTAAAACAGCGTTGACAGAAGCCa                                                         >  2:690382/1‑150 (MQ=255)
                                                                                              tgtgTTGCACACAGTTATCTGTGAGAAACCCATGGAAGAGAAATGGAAATAAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCATTAATCTTTAAAACAGCGTTGACAGAAGCCATaa                                                      >  1:113533/1‑150 (MQ=255)
                                                                                                tgtTGCACACAGTTATCTGTGAGAAACCCATGAAGAGAAATGGAAATAAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCATTAATCTTTAAAACAGCGTTGACAGAAGCCATAAAAt                                                   >  2:528702/1‑150 (MQ=255)
                                                                                                    gCACACAGTTATCTGTGAGAAACCCATGGAAGAGAAATGGAAATAAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCATTAATCTTTAAAACAGCGTTGACAGAAGCCATAAAATAAg                                                >  2:518993/1‑150 (MQ=255)
                                                                                                     cacacaGTTATCTGTGAGATACCCATGGAAGAGAAATGGAAATAAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCATTAATCTTTAAAACAGCGTTGACAGAAGCCATAAAATAAGc                                               <  2:817932/150‑1 (MQ=255)
                                                                                                       cacaGTTATCTGTGAGAAACCCATGGAAGAGAAATGGAAATAAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCATTAATCTTTAAAACAGCGTTGACAGAAGCCATAAAATAAGCaa                                             >  1:147581/1‑150 (MQ=255)
                                                                                                         caGTTATCTGTGAGAAACCCATGGAAGAGAAATGGAAATAAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCATTAATCTTTAAAACAGCGTTGACAGAAGCCATAAAATAAGCAAcc                                           <  1:880937/150‑1 (MQ=255)
                                                                                                          aGTTATCTGTGAGAAACCCATGGAAGAGAAATGGAAATAAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCATTAATCTTTAAAACAGCGTTGACAGAAGCCATAAAATAAGCAACCg                                          >  1:835979/1‑150 (MQ=255)
                                                                                                           gTTATCTGTGAGAAACCCATGGAAGAGAAATGGAAATAAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCATTAATCTTTAAAACAGCGTTGACAGAAGCCATAAAATAAGCAACCGa                                         >  2:143115/1‑150 (MQ=255)
                                                                                                                cTGTGAGAAACCCATGGAGGAGAAATGGAAATAAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCcaca                                                                                                         >  1:322149/1‑81 (MQ=255)
                                                                                                                cTGTGAGAAACCCATGGAGGAGAAATGGAAATAAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCcaca                                                                                                         <  2:322149/81‑1 (MQ=255)
                                                                                                                cTGTGAGAAACCCATGGAAGAGAAATGGAAATAAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCATTAATCTTTAAAACAGCGTTGACAGAAGCCATAAAATAAGCAACCGAAcgtt                                    >  1:181486/1‑150 (MQ=255)
                                                                                                                   tgAGAAACCCATGGAAGAGAAATGGAAATAAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGCTCGCATTAATCTTTAAAACAGCGTTGACAGAAGCCATAAAATAAGCAACCGAAcgttcgt                                 >  1:922255/1‑150 (MQ=255)
                                                                                                                    tataaaCCCATGGAAGAGAATTGGAAATAAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCATTAATCTTTAAAACAGCGTTGACAGAATCCATAAAATAAGCAACCGAAcgttcgtt                                <  2:756122/147‑1 (MQ=255)
                                                                                                                    gggaAACCCATGGAAGAGAAATGGAAATAAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCATTAATCTTTACAACAGCGTTGACAGAAGCCATAAAATAAGCAACCGAAcgttcgtt                                >  1:184784/3‑150 (MQ=255)
                                                                                                                      gaAACCCATGGAAGAGAAATGGAAATAAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCATTAATCTTTAAAACAGCGTTGACAGAAGCCATAAAATAAGCAACCGAACGttcgttcg                              >  2:722195/1‑150 (MQ=255)
                                                                                                                        aaCCCATGGAAGAGAAATGGAAATAAAGAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCATTAATCTTTAAAACAGCGTTGACAGAAGCCATAAAATAAGCAACCGAACGTTCGTTCGAg                            <  2:1141272/150‑1 (MQ=255)
                                                                                                                        aaCCCATGGAAGAGAAATGGAAATAAAGAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCATTAATCTTTAAAACAGCGTTGACAGAAGCCATAAAATAAGCAACCGAACGTTCGTTCGAg                            <  2:1136196/150‑1 (MQ=255)
                                                                                                                        aaCCCATGGAAGAGAAATGGAAATAAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCATTAATCTTTAAAACAGCGTTGACAGAAGCCATAAAATAAGCAACCGAACGTTCGTTCGAg                            >  2:853543/1‑150 (MQ=255)
                                                                                                                        aaCCCATGGAAGAGAAATGGAAATAAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCATTAATCTTTAAAACAGCGTTGACAGAAGCCATAAAATAAGCAACCGAACGTTCGTTCGAg                            <  1:607610/150‑1 (MQ=255)
                                                                                                                          cccATGGAAGAGAAATGGAAATAAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAAAACCCACAGCGTGATATAGATCGAATAAATCTTTAAAACAGCGTTGACAGAAGCCATAAAATAAGCAACCGAACGTACGTTCAAGCg                          >  2:19270/1‑150 (MQ=255)
                                                                                                                             aTGGAAGAGAAATGGAAATAAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCATTAATCTTTAAAACAGCGTTGACAGAAGCCATAAAATAAGCAACCGAACGTTCGTTCGAGCGTAt                       >  2:312708/1‑150 (MQ=255)
                                                                                                                                  agagAAATGGAAATTAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCATTAATCTTTAAAACAGCGTTGACAGAAGCCATAAAATAAGCAACCGAACGTTCGTTCGAGCGTATTgggg                  >  1:125044/1‑149 (MQ=255)
                                                                                                                                    agaAATGGAAATAAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCATTAATCTTTAAAACAGCGTTGACAGAAGCCATAAAATAAGCAACCGAACGTTCGTTCGAGCGTATTGGGAAc                <  1:1050484/150‑1 (MQ=255)
                                                                                                                                           taaaTAAAAAGTACTGAAATATCGATCAAAAAACCAAAAACTGCAATACCCACAGCTTTATATAGATCGCATTAATCTTTAAAACAGCGTTGACAGAAGCCATAAAATAAGCAACCGAACGTTCGTTCGTGCGTATTGGGAACGATAAAc         <  1:19270/149‑1 (MQ=255)
                                                                                                                                           gAAATAAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCATTAATCTTTAAAACAGCGTTGACAGAAGCCATAAAATAAGCAACCGAACGTTCGTTCGAGCGTATTGGGAACGATAAAc         <  2:324423/150‑1 (MQ=255)
                                                                                                                                             aaTAAAAAGACCTGCCACCGCTCTCAAAAACACAAAAACTGCAATACCCACAGCGTGATATAGATCTCATTAATCTTTAAAACAGCTTTGACAGAAGCCATAAAATAAGCAACCGAACGTTCGTGCGAGCGTATTGGGAACGATAAActc       <  2:193574/150‑1 (MQ=255)
                                                                                                                                               tAAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCATTAATCTTTAAAACAGCGTTGACAGAAGCCATAAAATAAGCAACCGAACGTTCGTTCGAGCGTATTGGGAACGATAAACtctc     >  1:1141358/1‑150 (MQ=255)
                                                                                                                                                 aaaaGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCATTAATCTTTAAAACAGCGTTGACAGAAGCCATAAAATAAGCAACCGAACGTTCGTTCGAGCGTATTGGGAACGATAAACTCTCAt   >  1:342167/1‑150 (MQ=255)
                                                                                                                                                 aaaaGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCATTAATCTTTAAAACAGCGTTGACAGAAGCCATAAAATAAGCAACCGAACGTTCGTTCGAGCGTATTGGGAACGATAAACTCTCAt   >  1:892908/1‑150 (MQ=255)
                                                                                                                                                 aaaaGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCATTAAACTTTAAAACAGCGTTGACAGAAGCCATAAAATAAGCAACCCAACGTTCGATCGAGCGAAATGCCAACGATAAACTCTCa  >  1:337263/1‑149 (MQ=255)
                                                                                                                                                  aaaaaaCTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCATTAATCTTTAAAACAGCGTTGACAGAAGCCATAAAATAAGCAACCGAACGTTCGTTCGAGCGTATTGGGAACGATAAACTCTCATg  <  1:690382/150‑1 (MQ=255)
                                                                                                                                                  aaaGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCATTAATCTTTAAAACAGCGTTGACAGAAGCCATAAAATAAGCAACCGAACGTTCGTTCGAGCGTATTGGGAACGATAAACTCTCATg  <  1:427169/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TCACGGGTTGGGGTTTCTACAGGACGTAACATAAGGGACTCCTCATTAAGATAATAATACTGGTCAACCTTTAATCTGATTAGTTAACCAATTCTGTGTTGCACACAGTTATCTGTGAGAAACCCATGGAAGAGAAATGGAAATAAAAAGAACTGACACCGCGATCAAAAAACCAAAAACTGCAATACCCACAGCGTGATATAGATCGCATTAATCTTTAAAACAGCGTTGACAGAAGCCATAAAATAAGCAACCGAACGTTCGTTCGAGCGTATTGGGAACGATAAACTCTCATG  >  NC_000913/1696040‑1696335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: