Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,709,006 | A→G | 21.5% | A696A (GCA→GCG) | rsxC → | SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,709,006 | 0 | A | G | 21.5% | 46.3 / 14.5 | 42 | A696A (GCA→GCG) | rsxC | SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex |
Reads supporting (aligned to +/- strand): ref base A (16/11); new base G (4/4); total (22/20) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.00e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.35e-01 |
GTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAG > NC_000913/1708860‑1709152 | gTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAACCGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTAGATCCGCGCAACGCGGCCGTGGCAGCGGCGAGTGCACGCGCTAAGGCCCga > 1:214084/1‑149 (MQ=14) aTCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGc > 1:402801/1‑150 (MQ=255) cgcAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGaa > 1:947775/1‑150 (MQ=255) gcAAAGCCGCCGTCTAAGCCGCTATTGCCCGATCCAAAGCGCGCAACCGGGAACAGCAACAGGCTAATGCGGATCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAAc < 1:977172/150‑1 (MQ=14) gTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCt > 1:1107302/1‑150 (MQ=255) gAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGCAGCGGCTATTGCCCGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTaaa > 2:879677/1‑149 (MQ=255) cTATTTCCCGCGCCAAAGCGCTCACTCGGTAACAGCAACCGCCTATTGCGGAGCCACTAGCTCAGGTCGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTAc < 1:1071516/150‑1 (MQ=17) ccGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCCCGCAAGCTGGAACAGCCACACGCTAATGCGGTACCagcaga > 1:139692/1‑150 (MQ=255) acaGCGCGCAACGGGGAACAGCAACCGGCTAATGAGGACGCAGAACACCTGGTTGATCCGCGAAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGtt < 1:1188501/148‑1 (MQ=17) cgcAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCcg > 1:1055256/1‑150 (MQ=255) cAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTAGACGCGGCTATTGCACGCGCAAAAGCACGCAAGCAGGACCAGCAGCAGGCTAATGCGGTACCAGAAGAACAGGTTGATCagcc > 2:878263/1‑149 (MQ=37) cAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGtt > 1:258391/1‑150 (MQ=255) ggcaactGCCGAGCCAGCACCCCAGGCCGAGCCGCGCAAACCCGCCGTCGACGCCGCTATTGCACGCGCCACAGCCCGCAAGCTGGAACAGCAACAGGCTAATGCGGGACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCcgc < 1:322144/144‑1 (MQ=14) ggCTAATTCGGACGGAGCATAGCAGGTTGATCCTCGTAAATCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCTCGCAAGCTGGAACAGCAACAGGCTATTGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCcgc < 1:696677/150‑1 (MQ=17) ggCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCTCAAATGCAAGAATGATGTTAAAGCATCAGGTTAATGAGGTAGCAGAAGAGCAGGTTGATCCGCGCAGAGCGGCAGTTGCcgc > 2:661388/1‑150 (MQ=25) cGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTg < 1:1197025/150‑1 (MQ=255) ggcgCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCCCGCGCCCAAGCACGCAAGCTGGAACAGCAACAGGCTAATGAGGTACCAGAAGACCAGGTAGCTCCGCGCAACGCGGCAGTTGCCGCGGCTATTGc > 2:674791/4‑150 (MQ=18) cAGAAGCCCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCcgcg < 1:391526/150‑1 (MQ=255) cAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAATAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCAATTGCCcgcg > 2:101123/1‑150 (MQ=255) cccgttgTTGATCCTCGTAAAGCTGCCGTAGAAGCCGCTATTGCACGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAg < 2:60705/144‑1 (MQ=18) cAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGACAAAGCACGCAAGCTGGAACAGAAACAGGCTAATTCGGTACCAGATGAACAGGTTCAACCGCGCAAAGCGGCAGTTGACGCGGAAATTGCACGCGCTCAGGcc > 1:946206/1‑150 (MQ=21) tggTCGATCCTCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCa < 2:1090721/149‑1 (MQ=11) tGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCaaaaa > 2:281782/1‑150 (MQ=255) aaaGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCAGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCcag > 1:116265/1‑150 (MQ=255) tgCTGCCGGCAAAGACGATATTGCACCTGCCTAAGAACGCAAGCTGGAACAGCAACAGGCTAATGCGGCCCCAGAAGAACAGGTCGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCcagca < 1:66908/149‑1 (MQ=18) aGCTGCCTTCTAAGCCGCTTTTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCcagca < 1:757306/150‑1 (MQ=255) aGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGGAAGCAGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGCTCCGCGCAAAGCGGCAGTTCCCGCGGCTATTGCACGCGCTCAGGCCAAAAAAGCCGCCcagca > 1:336859/1‑150 (MQ=25) cTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACCGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGa < 1:869914/150‑1 (MQ=255) ggCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACgt > 2:237556/1‑149 (MQ=255) cgCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTAGATCCGCGCAAAGCGGCAGTAGCCGCGGCTATTGCCCGAGCTAAGGCCAAAAAACCCGCACAGCAGCAGGCTGTAAACGa > 2:1027145/2‑150 (MQ=17) gCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTAACAGAAGAACAGGTTGATCCTCTCAAAGCGGCAGTTGCCGCTGCTATTGCCCTCGCTCAGGCAAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAg < 1:1209455/150‑1 (MQ=25) cTATTGCCCGTGCCAAAGCTCGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAgg < 1:1027145/150‑1 (MQ=255) ttccgctcgcCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACt < 1:68345/143‑1 (MQ=255) tGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTa > 1:1180697/1‑150 (MQ=255) cgcgCCAAAGCCCGCAAGCTGGAACAGCAACAGGCTAAGGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATg < 1:56229/150‑1 (MQ=255) gcgcCAAAGCACGCAAGCTGGAACCGCAACTGGCTAATTCGGTTCCAGAAGTACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATgg < 2:214084/150‑1 (MQ=38) ccAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCcgcg > 2:177806/1‑64 (MQ=25) ccAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCcgcg < 1:177806/64‑1 (MQ=25) cAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTAGATCCGCGCAAAGCCGCAGTTGCCGCGGCTATTGCCCGCGCACAGGCCAAAAAAGCCGAACAGCAGAAGGATGAAAACGAGGACTAAAAGGTAtt > 1:892279/1‑150 (MQ=18) cAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTAGATCCGCGCAAAGCGGCCGTAGCCGCGGCTATTGCCCGCGCGCAGGCCAACAAAGCCGCCCAGCAGCAGGGTGTAACCGAGGACGAAAAGGTagt > 1:469875/1‑148 (MQ=17) aaGCCCGCACGCGCGAACAGCTACCTTCTAATGCGGAACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCa < 1:892725/150‑1 (MQ=37) aGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAg < 2:947775/150‑1 (MQ=255) | GTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAG > NC_000913/1708860‑1709152 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |