Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,727,000 A→G 7.4% Q114Q (CAA→CAG nemA → chromate reductase, quinone reductase, FMN‑linked; N‑Ethylmaleimide reductase; old yellow enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,727,0000AG 7.4% 185.6 / 6.2 68Q114Q (CAA→CAGnemAchromate reductase, quinone reductase, FMN‑linked; N‑Ethylmaleimide reductase; old yellow enzyme
Reads supporting (aligned to +/- strand):  ref base A (35/27);  new base G (2/3);  total (38/30)
Fisher's exact test for biased strand distribution p-value = 6.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.50e-01

CAGGCAAAAGGATATGCAGGTGCGCCTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACACGTACTTCTCTGCGCGATGAAAATGGTCAGGCGATCCGTGTTGAAACATCCATGCCGCGTGCGCTTGAACTGGAAGAGATTCCAGGTATCGTCAA  >  NC_000913/1726851‑1727143
                                                                                                                                                     |                                                                                                                                               
cAGGCAAAAGGATATGCAGGTGCGCCTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCaa                                                                                                                                                 >  1:22390/1‑150 (MQ=255)
 aGGCAAAAGGATATGCAGGTGCGCCTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAAc                                                                                                                                                >  1:1009118/1‑150 (MQ=255)
    cAAAAGGATACGCAGGTGCGCCCGGCATCCATAGTCCGGAGCAAATAGCCGCATGGAACAAAATCACCGCGGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTg                                                                                                                                             <  2:841168/150‑1 (MQ=255)
      aaaGGATATGCAGGTGCGCCTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGc                                                                                                                                           <  2:257547/150‑1 (MQ=255)
         ggATATGCAGGTGCGCCTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGGTTGCAGGCTGGCgtg                                                                                                                                        <  1:437773/150‑3 (MQ=255)
         ggAGATGCAGGTGCGCCTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGGTTGCAGGCTGGCgtg                                                                                                                                        <  2:1223997/150‑3 (MQ=255)
          gATATGCAGGTGCGCCTGGCATGCATAGTCCGGCGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGGTTGCAGGCTGGCgtga                                                                                                                                       <  2:499244/150‑4 (MQ=255)
          gATATGCAGGTGCGCCTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAATATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTc                                                                                                                                       >  1:916097/1‑150 (MQ=255)
                    tGCGCCTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGt                                                                                                                             <  1:503529/150‑1 (MQ=255)
                       gcCTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCGGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAgc                                                                                                                          <  1:463083/150‑1 (MQ=255)
                       gcCGGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCGGGCGATCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAgc                                                                                                                          <  1:458498/150‑1 (MQ=255)
                        ccTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCCGGCTCCGGTAgcg                                                                                                                         >  2:1302886/1‑150 (MQ=255)
                              agccATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCa                                                                                                                   <  1:814392/148‑1 (MQ=255)
                                     gTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTa                                                                                                            >  1:26583/1‑150 (MQ=255)
                                       ccGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAgc                                                                                                          <  1:394168/150‑1 (MQ=255)
                                         ggAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAgcgc                                                                                                        <  1:793598/150‑1 (MQ=255)
                                          gAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTcgcgcg                                                                                                       <  2:546205/150‑1 (MQ=255)
                                          gAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGcgcg                                                                                                       <  2:132964/150‑1 (MQ=255)
                                           aGCAACTTGCCGCATCGAAAAAAATCACCGCTGGCCTTCATGCTGAACATGCTCATCGGGCCGCCCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTACCGCgg                                                                                                      <  1:768876/150‑1 (MQ=255)
                                             cAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGa                                                                                                    >  2:1116681/1‑150 (MQ=255)
                                                   gCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCCCCGGTAGCGCCGGCAGCACTTAGCGCGGGAACACGt                                                                                              >  1:49068/1‑150 (MQ=255)
                                                      gCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACACGTACt                                                                                           >  1:453247/1‑150 (MQ=255)
                                                      gCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACACGTACt                                                                                           >  2:700117/1‑150 (MQ=255)
                                                        aTGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACACGTACTtc                                                                                         <  1:899771/150‑1 (MQ=255)
                                                        aTGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGGCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACACGTACTtc                                                                                         <  1:1155785/150‑1 (MQ=255)
                                                               aaaaTCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACACGTACTTCTCTgcgc                                                                                  >  1:462330/1‑150 (MQ=255)
                                                                 aaTCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACACGTACTTCTCTGCGCGa                                                                                >  2:944741/1‑150 (MQ=255)
                                                                       cGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACCCGTACTTCTCTGCGCGATGaaaa                                                                          >  1:948498/1‑150 (MQ=255)
                                                                         cTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACACGTACTTCTCTGCGCGATGAAAATg                                                                        >  2:812029/1‑150 (MQ=255)
                                                                         cTGGCGGTCATGCTGAAACTGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACACGTACTTCTCTGCGCGATGAAAATg                                                                        <  1:987877/150‑1 (MQ=255)
                                                                           ggCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACACGTACTTCTCTGCGCGATGAAAATGGt                                                                      <  1:909808/150‑1 (MQ=255)
                                                                           ggCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACACGTACTTCTCTGCGCGATGAAAATGGt                                                                      <  1:904956/150‑1 (MQ=255)
                                                                                  aTGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCCGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACACGTACTTCTCTGCGCGATGAAAATTGTCAGGCGa                                                               >  2:828949/1‑150 (MQ=255)
                                                                                  aTGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTTGCGCCTTCAGCACTTAGCGCGGGAACACGTACTTCTCTGCGCGATGAAAATGGTCAGGCGa                                                               >  1:1140866/1‑150 (MQ=255)
                                                                                   tGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCACCCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACACGTACTTCTCTGCGCGATGAAAATGGTCAGGCGAt                                                              >  2:793246/1‑150 (MQ=255)
                                                                                   tGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACACGTACTTCTCTGCGCGATGAAAATGGTCAGGCGAt                                                              >  2:652377/1‑150 (MQ=255)
                                                                                     cTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACACGTACTTCTCTGCGCGATGAAAATGGTCAGGCGATcc                                                            >  1:319091/1‑150 (MQ=255)
                                                                                        aaaaTGGGCATATGGGCGTGAAGATGTGGCACACCGGACGCATTTCTCATGCCAGCCTGCAACCTGTCGGTCTGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACACGTTCTTCTCTGCGCGATGAAAATGGTCAGGCGATCCgtg                                                         <  1:973080/150‑1 (MQ=255)
                                                                                         aaaTGGTCATATGGCCGTTCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACACGTACTTCTCTGCGCGATGAAAATGGTCAGGCGATCCgtgt                                                        <  2:1100441/150‑1 (MQ=255)
                                                                                           aTGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACACGTACTTCTCTGCGCGATGAATATGGTCAGGCGATCCGTGTTg                                                      >  1:1089068/1‑150 (MQ=255)
                                                                                             ggTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACACGTACTTCTGTGCCCGATGAAAATGGTCAGGCGATCCGTGTTGaa                                                    >  1:400832/1‑150 (MQ=255)
                                                                                                cATAAGGCCGTCCAGCTGGGGAACACCGGACGCATTTCTCACGCCAGCCTTCAACCTGTCGGTCAGGCACCGGTAGCGCCTTCCGCACTTAGCGCGGGAACACGTACTGCTCTGCGCGATGAAAATGGTCAGTCGATCCGTGTTGAcaca                                                 <  2:939388/150‑1 (MQ=255)
                                                                                                      gCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACACGTACTTCTCTGCGCGATGAAAATGGTCAGGCGATCCGTGTTGAAACATCCATg                                           <  1:461860/150‑1 (MQ=255)
                                                                                                       ccGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCCGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCGTCAGCACTTAGCGCGGGAACACGTACTTCTCTGCGCGATGAAAATGGTCAGGCGCTCCGTGTTGAAACATCCATGc                                          >  1:27144/1‑150 (MQ=255)
                                                                                                        cGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACACGTACTTCTCTGCGCGATGAAAATGGTCAGGCGATCCGTGTTGAAACATCCATGcc                                         <  2:677260/150‑1 (MQ=255)
                                                                                                           gCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACACGTACTTCTCTGCGCGATGAAAATGGTCAGGCGATCCGTGTTGAAACATCCATGCcgcg                                      >  1:900012/1‑150 (MQ=255)
                                                                                                                   ggCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACACGTACTTCTCTGCGCGATGAAAATGGTCAGGCGATCCGTGTTGAAACATCCATGCCGCGTGCGCTTg                              <  1:733004/150‑1 (MQ=255)
                                                                                                                   ggCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACACGTACTTCTCTGCGCGATGAAAATGGTCAGGCGATCCGTGTTGAAACATCCATGCCGCGTGCGCTTg                              >  2:1037826/1‑150 (MQ=255)
                                                                                                                     cacaCCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACACGTACTTCTCTGCGCGATGAAAATGGTCAGGCGATCCGTGTTGAAACATCCATGCCGCGGGCGCTAGaa                            >  1:853739/1‑150 (MQ=255)
                                                                                                                     cacaCCGGACGCATATCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTGCAGCACTTAGCGCGCGAACACGTACTTATCTGCGCGATGAAAATGGTCAGGCGATCCGTGAGGAAACATCCAAGCCGCGTGCGCTTGaa                            >  2:1284484/1‑150 (MQ=255)
                                                                                                                         ccGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTGCAGCACTTAGCGCGGGAACACGTACTTCTCTGCGCGATGAAAATGGTCAGGCGATCCGGGTGGAAACATCCATGCCGCGTGCGCTTGACCTgg                        >  1:621266/1‑150 (MQ=255)
                                                                                                                              cGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACACGTACTTCTCTGCGCGATGAAAATGGTCAGGCGATCCGTGTTGAAACATCCATGCCGCGTGCGCTTGAACTGGAagag                   <  1:1116681/150‑1 (MQ=255)
                                                                                                                                cATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACACGTACTTCTCTGCGCGATGAAAATGGTCAGGCGATCCGTGTTGAAACATCCATGCCGCGTGCGCTTGAACTGGAAGAGAt                 <  1:548851/150‑1 (MQ=255)
                                                                                                                                cATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACACGTACTTCTCTGCGCGATGAAAATGGTCAGGCGATCCGTGTTGAAACATCCATGCCGCGTGCGCTTGAACTGGAAGAGAt                 <  1:63516/150‑1 (MQ=255)
                                                                                                                                 aTTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCCGCACTTAGCGCGGGAACACGTACTTCTCTGCGCGATGAAAATGGTCAGGCGATCCGTGTTGAAACATCCATGCCGCGTGCGCTTGAACTGGAAGAGAtt                >  2:35137/1‑150 (MQ=255)
                                                                                                                                 aTTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACACGTACTTCTCTGCGCGATGAAAATGGTCAGGCGCTCCGTGTTGAAACATCCCTGCCGCGTGCGCTTGAACTGGAAGAGAtt                >  1:787508/1‑150 (MQ=255)
                                                                                                                                 aTTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACACGTACTTCTCTGCGCGATGAAAATGGTCAGGCGATCCGTGTTGAAACATCCATGCCGCGTGCGCTTGAACTGGAAGAGAtt                >  2:35136/1‑150 (MQ=255)
                                                                                                                                  tttCTCACGGCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCACGGGAACACGTACTTCTCTGCGCGATGAAAATGGTCAGGCGATCCGTGTTGAAACATCCATGCCGCGTGCGCTTGTACTGGAAGAGATTc               <  2:722877/150‑1 (MQ=255)
                                                                                                                                   ttCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCCGCACTTAGCGCGGGAACACGTACTTCTCTGCGCGATGAAAATGGTCAGGCGATCCGTGTTGAAACATCCATGCCGCGTGCGCTTGAACTGGAAGAGAGTcc              >  2:834258/1‑150 (MQ=255)
                                                                                                                                    ctgaccGCCAGGTTGCAGGCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACACGTACTTCTCTGCGCGATGAAAATGGTCAGGTGATCCGTGTTGAAACATCCATGCCGCGTGCGCTTGAACTGGAAGAGATTCCa             >  1:1273008/6‑150 (MQ=255)
                                                                                                                                     tgaccGCCAGGTTGCAGGCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACACGTACTTCTCTGCGCGATGAAAATGGTCAGGCGATCCGTGTTGAAACATCCATGCCGCGTGCGCTTGAACTGGAAGAGATTCCAg            >  1:902028/5‑150 (MQ=255)
                                                                                                                                      tcACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGTACACGTTCTTCTCTGCGCGATGAAAATGGTCAGGCGATCCGTGTTGAAACATCCTTGCCGCGTGCGCTTGACCTGGAAGAGATTCCAgg           >  2:646416/1‑150 (MQ=255)
                                                                                                                                      tcACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACACGTACTTCTCTGCGCGATGAAAATGGTCAGGCGATCCGTGTTGAAACATCAATGCCGCGTGCGCTTGAACTGGAAGAGATTCCAgg           >  2:642543/1‑150 (MQ=255)
                                                                                                                                         cGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACACGTACTTCTCGGCGCGATGAAAATGGTCAGGCGATCCGGGTTGAAACATCCATGCCGCGTGCGCTTGAACTGGAAGAGATTCAAGGTAt        >  1:665181/1‑150 (MQ=255)
                                                                                                                                            ccgCCTGCAACCTGGCGGTCAGGCACCGGTAGAGCCTTCAGCACTTAGCGCGGGAACACGTACTTCTCTGCGCGATGAAAATGGTCAGGCGATCCGTGTTGAAACATCCATGCCGCGTGCGCTTGAACTGGAAGAGATTCCAGGTATCGt     >  2:261562/3‑150 (MQ=255)
                                                                                                                                             aGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACACGTACTTCTCTGCGCGATGAAAATGGTCAGGCGATCCGTGTTGAAACATCCATGCCGCGTGCGCTTGAACTGGAAGAGATTCCAGGTATCGTc    >  1:1260577/1‑150 (MQ=255)
                                                                                                                                             aGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACACGTACTTCTCTGCGCGATGAAAATGGTCAGGCGATCCGTGTTGAAACATCCATGCCGCGTGCGCTTGAACTGGAAGAGATTCCAGGTATCGTc    <  2:1017819/150‑1 (MQ=255)
                                                                                                                                              gCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACACGTACTTCTCTGCGCGATGAAAATGGTCAGGCGATCCGTGTTGAAACATCCATGCCGCGTGCGCTTGAACTGGAAGAGATTCCAGGTATCGTCa   >  1:716402/1‑150 (MQ=255)
                                                                                                                                               ccTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACACGTACTTCTCTGCGCGATGAAAATGGTCAGGCGATCCGTGTTGAAACATCCATGCCGCGTGCGCTTGAACTGGAAGAGATTCCAGGTATCGTCaa  >  1:1065461/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                               
CAGGCAAAAGGATATGCAGGTGCGCCTGGCATCCATAGTCCGGAGCAAATTGCCGCATGGAAAAAAATCACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTGCAGCTGTGGCACACCGGACGCATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACACGTACTTCTCTGCGCGATGAAAATGGTCAGGCGATCCGTGTTGAAACATCCATGCCGCGTGCGCTTGAACTGGAAGAGATTCCAGGTATCGTCAA  >  NC_000913/1726851‑1727143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: