Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,777,968 A→G 5.7% E258G (GAA→GGA)  ydiO → putative acyl‑CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,777,9680AG 5.7% 204.5 / 2.7 70E258G (GAA→GGA) ydiOputative acyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (33/32);  new base G (2/2);  total (35/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

CTGCGAAGTCTATCTCGACAACGTGGAGGTTGAAGAGAGCGACATGGTGGGCGAAGAAGGAATGGGTTTCCTCAATGTGATGTACAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGGCGCTGATGGCGATTAAGATCGACAACATGCGCAACATGGTGCTGAAAGTGGCATGGCAAGCCGATCAG  >  NC_000913/1777819‑1778116
                                                                                                                                                     |                                                                                                                                                    
cTGCGAAGTCTATCTCGACAACGTGGAGGTTGAAGAGAGCGACATGGTGGGCGAAGAAGGAATGGGTTTCCTCAATGTGATGTACAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGa                                                                                                                                                      >  1:1020077/1‑150 (MQ=255)
   cGAAGTCTATCTCGACAACGTGGAGGTTGAAGAGAGCGACATGGTGGGCGAAGAAGGAATGGGTTTCCTCAATGTGATGTACAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGa                                                                                                                                                   >  2:1050813/1‑150 (MQ=255)
      aGTCTATCTCGACAACGTGGAGGTTGAAGAGAGCGACATGGTGGGCGAAGAAGGAATGGGTTTCCTCAATGTGATGTACAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATgc                                                                                                                                                >  1:77477/1‑150 (MQ=255)
          aaTCTCGCCCACGTGGAGGAGCAAGAGAGCGACATGGAGGGCGAAGAAGGAATGGGTTTACTCAATGTGAGGTACAACGTTGAGATGGAGCGCCTGAGCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGCAGATgccgcc                                                                                                                                            <  2:944803/149‑1 (MQ=255)
            tctcGACAACGTGGAGGTTGAAGAGAGCGACATGGTGGGCGAAGAAGGAATGGGTTTCCTCAATGTGATGTACAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGAAGccgcacg                                                                                                                                          >  1:868537/1‑150 (MQ=255)
               cGACAACGTGGAGGTTGAAGAGAGCGACATGGTGGGCGAAGAAGGAATGGGTTTCCTCAATGTGATGTACAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTa                                                                                                                                       >  1:1028109/1‑150 (MQ=255)
                 aCAACGTGGAGGTTGAAGAGAGCGACATGGTGGGCGAAGAAGGAATGGGTTTCCTCAATGTGATGTACAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATg                                                                                                                                     >  1:1267235/1‑150 (MQ=255)
                  cAACGTGGAGGTTGACGAGAGCGACATGGTGGGCGAAGAAGGAATGGGTTGCCTCAATGTGATGTACAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGc                                                                                                                                    <  2:342323/150‑1 (MQ=255)
                   aaCGTGGAGGTTGAAGAGAGCGACATGGTGGGCGAAGAAGGAATGGGTTTCCTCAATGTGATGTACAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGcc                                                                                                                                   <  1:777478/150‑1 (MQ=255)
                       tGGAGGTTGAAGAGAGCGACATGGTGGGCTAAGAAGGAATGGGTTTCCTCAATGTGATGTACAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGGAGATGCCGCCCGCTATGccaacc                                                                                                                               >  2:155123/1‑150 (MQ=255)
                       tGGAGGTTGAAGAGAGCGACATGGTGGGCGAAGAAGGAATGGGTTTCCTCAATGTGATGTACAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGGAGATGCCGCCCGCTAAGccaacc                                                                                                                               >  2:150855/1‑150 (MQ=255)
                           ggTTGAAGAGAGCGACATGGTGGGCGAAGAAGGAATGGGTTTCCTCAATGTGATGTACAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAACCAACg                                                                                                                           >  2:1291488/1‑150 (MQ=255)
                              tGAAGAGAGCGACATGGTGGGCGAAGAAGGAATGGGTTTCCTCAAGGTGATGTACAACATTGAGATGGAGCGACTGATCAACGCCGCGCGCAGCACCGCCATTGCAGAATGCGCATGTGAAGATGCCGCCCGCTCTGCCAACCAACGTaa                                                                                                                        >  1:512096/1‑149 (MQ=255)
                                  gagagCGACATGGTGGGCGAAGAAGGAATGGGTTTCCTCAATGTGATGTACAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCt                                                                                                                    <  2:1249869/150‑1 (MQ=255)
                                     agCGACATGGTGGGCGAAGAAGGAATGGGTTTCCTCAATGTGATGTACAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCtttt                                                                                                                 <  1:614980/150‑1 (MQ=255)
                                          cATGGTGGGCGAAGAAGGAATGGGTTTCCTCAATGTGATGTACAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTaa                                                                                                            >  2:129634/1‑150 (MQ=255)
                                           aTGGTGGGCGAAGAAGGAATGGGTTTCCTCAATGTGATGTACAACTTTGAGATGGAGCGCCTGATCTACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAg                                                                                                           <  1:855918/150‑1 (MQ=255)
                                            tggtggGCGAAGAAGGAATGGGTTTCCTCAATGTGATGTACAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAGc                                                                                                          >  2:679443/1‑150 (MQ=255)
                                              gtggGCGAATAAGGAATGGGTTTCCTCATTGTGATGTACAACTTTGAGATCGAGCGCCTGATCAACGCCGCGCGCAGCAGCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATGGCTTTTGGTAAGccc                                                                                                        <  2:799950/150‑1 (MQ=255)
                                                gggCGATGAAGGAATGGGTTTCCTCAATGTGATGTACAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGCTGCCGCCCGCTATGCCACCCCACGGATCGCTTTTGGTAAGCCCAt                                                                                                      >  1:397017/1‑150 (MQ=255)
                                                 ggCGAAGAAGGAATGGGTTTCCTCAATGTGATGTACAACTTTGATATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGTCAACCAACGTATCGCTTTTGGTAAGCCCAtt                                                                                                     <  1:380076/150‑1 (MQ=255)
                                                 ggCGAAGAAGGAATGGGTTTCCTCAATGTGATGTACAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAGCCCAtt                                                                                                     <  2:688146/150‑1 (MQ=255)
                                                  gCGAAGAAGGAATGGGTTTCCTCAATGTGATGTACAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAGCCCATTg                                                                                                    >  2:464101/1‑150 (MQ=255)
                                                     aagaagGAATGGGTTTCCTCAATGTGATGTACAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTAGTAAGCCCATTGGTc                                                                                                 <  1:557408/150‑1 (MQ=255)
                                                         agGAATGGGTTTCCTCAATGTGATGTACAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATaa                                                                                             >  2:1083431/1‑150 (MQ=255)
                                                          ggAATGGGTTTCCTCAATGTGATGTACAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAAc                                                                                            <  1:813297/150‑1 (MQ=255)
                                                          ggAATGGGGTTCCTCAATGTGATGTACAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAAc                                                                                            <  1:649013/150‑1 (MQ=255)
                                                                ggTTTCCTCAATGTGATGTACAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAgatg                                                                                      <  2:1209844/150‑1 (MQ=255)
                                                                ggTTTCCTCAATGTGATGTACAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAgata                                                                                      >  1:509511/1‑149 (MQ=255)
                                                                ggTTTCCTCAATGGGATGTACAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAgatg                                                                                      <  2:1193815/150‑1 (MQ=255)
                                                                    tCCTCAATGTGATGTACAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATcc                                                                                  <  1:129634/150‑1 (MQ=255)
                                                                     ccTCAATGTGATGTACAACTTTGAGATGGAGCGCCTGAGCAACGCCGCGCGCAGCACCTGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCa                                                                                 <  1:574122/150‑1 (MQ=255)
                                                                       tCAATGTGATGTACAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAgg                                                                               <  2:790304/150‑1 (MQ=255)
                                                                        aaaTGTGATGTACAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGa                                                                              <  2:1263576/149‑1 (MQ=255)
                                                                             tgATGTACAACTTTGAGATGGAGAGGCTGATCAAAGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGATGATGACTCCCGCTTTGCCAACCCACGTGTCGCTTTTGGTTAGCCAAGTGGTCCTAACCAGATGATCAAGGaaaacc                                                                         >  2:887886/1‑148 (MQ=255)
                                                                                tGTACAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCAAGGAACAACTgg                                                                      >  2:246329/1‑150 (MQ=255)
                                                                                 gTACAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGgc                                                                     <  1:908217/150‑1 (MQ=255)
                                                                                 gTACAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGgc                                                                     <  1:713078/150‑1 (MQ=255)
                                                                                  tccAATTTTGAGAGGGAGCTCCTGATCAACGCCGAGCGCAGCACCGGCTTGGCCGAATGCGCCTTTGAAGATTCCGCCCGCTATTCCAACCAACGTATCGCTTTTGGTAAGCCCATTTGTCATAACCAGATGATCCAGGAAAAACTGgcg                                                                    <  1:807250/148‑1 (MQ=255)
                                                                                  tACAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGgcg                                                                    >  2:1236062/1‑150 (MQ=255)
                                                                                  tACAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGgcg                                                                    >  1:630842/1‑150 (MQ=255)
                                                                                    cAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCCGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGGCGCt                                                                  >  1:979195/1‑150 (MQ=255)
                                                                                          tGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGGCGCTGATGGc                                                            <  2:725170/150‑1 (MQ=255)
                                                                                            agaTGTAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAACCACCGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGGCGCTGATGGCGa                                                          <  1:517623/150‑1 (MQ=255)
                                                                                              caggAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGAGGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGGCGCTGATGGCGAtt                                                        <  2:693836/148‑1 (MQ=255)
                                                                                               tGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCATCCAACGTATCGCTTTTGGTAAGCCCATTGGTCATTACCAGATGATCCAGGAAACACTGGCGCTGATGGCGATTa                                                       >  1:149346/1‑150 (MQ=255)
                                                                                                  aGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGGCGCTGATGGCGATTAAGa                                                    <  2:510289/150‑1 (MQ=255)
                                                                                                    cgcCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATTCGCCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATACCCAGATGATCCAGGAAAAACTGGCGCTGATGGCGATTAAGATc                                                  >  2:964510/1‑150 (MQ=255)
                                                                                                    cgcCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGGAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGGCGCTGATGGCGATTAAGATc                                                  <  1:155123/150‑1 (MQ=255)
                                                                                                    cgcCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGGAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAGCCCAATGGTCATAACCAGATGATCCAGGAAAAACTGGCGCTGATGGCGATTAAGATc                                                  <  1:150855/150‑1 (MQ=255)
                                                                                                    cgcCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAACACTGGCGCTGATGGCGATTAAGAtg                                                  >  1:818610/1‑149 (MQ=255)
                                                                                                     gcCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGGCGCTGATGGCGATTAAGATCg                                                 <  2:1113404/150‑1 (MQ=255)
                                                                                                      ccTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGACCCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTAGGAAAGCCCATTGGTCATAACCACATGCTCCAGGAAAAACTGGCGCGGATGGCGATTAAGCTCGa                                                >  1:248455/1‑150 (MQ=255)
                                                                                                       cTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGGCGCTGATGGCGATTAAGATCGac                                               <  2:12728/150‑1 (MQ=255)
                                                                                                        tGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGGCGCTGATGGCGATTAAGATCGaca                                              >  2:4443/1‑150 (MQ=255)
                                                                                                            cAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGGCGCTGATGGCGATTAAGATCGACAACAt                                          >  1:986122/1‑150 (MQ=255)
                                                                                                              aCGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGGCGCTGATGGCGATTAAGATCGACAACATgc                                        <  1:939076/150‑1 (MQ=255)
                                                                                                                gccgcGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGGCGCTGATGGCGATTAAGATCGACAACATgcgc                                      >  2:3757/1‑150 (MQ=255)
                                                                                                                gccgcGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGGCGCTGATGGCGATTAAGATCGACAACATgcgc                                      >  2:163144/1‑150 (MQ=255)
                                                                                                                       gcagcaCCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGGCGCTGATGGCGATTAAGATCGACAACATGCGCAACATgg                               <  1:16762/150‑1 (MQ=255)
                                                                                                                          gcaCCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGGCGCTGATGGCGATTAAGATCGACAACATGCGCAACATGGTGc                            >  2:1305102/1‑150 (MQ=255)
                                                                                                                            aCCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAACCTGGCGCTGATGGCTATTAAGATCTACAACATGCGCAACATGTTTCTg                          >  2:196226/1‑150 (MQ=255)
                                                                                                                            aCCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGGCGCTGATGGCGATTAAGATCGACAACATGCGCAACATGGTGCTg                          >  2:1074472/1‑150 (MQ=255)
                                                                                                                              cGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAAGCAACGTATCGCTTTTGGTACGCCCATTGGTCATAACCAGCTGCTCCAGGAAATACTGGCGCTGATGGCGATTAAGGTCGACAACATGCGCAACATGGTGCTgca                        >  1:284725/1‑148 (MQ=255)
                                                                                                                                    ttccGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGGCGCTGATGGCGATTAAGATCGACAACATGAGCAACATGGTGCTGAAAGtggc                  <  1:188141/148‑1 (MQ=255)
                                                                                                                                    tGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAGACCTGGCGCTGATGGCGATTAAGATCGACAACATGCGCAACATGGTGCTGAAAGtggc                  >  1:109952/1‑150 (MQ=255)
                                                                                                                                       cGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGGCGCTGATGGCGATTAAGATCGACAACATGCGCAACATGGTGCTGAAAGtggcatg               <  2:1020077/150‑1 (MQ=255)
                                                                                                                                             cgcCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGGCGCTGATGGCGATTAAGATCGACAACATGCGCAACATGGTGCTGAAAGTGGCATGGCAAGc         >  1:945291/1‑150 (MQ=255)
                                                                                                                                             cgcCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGGCGCTGATGGCGATTAAGATCGACAACATGCGCAACATGGTGCTGAAAGTGGCATGGCAAGc         <  1:1305102/150‑1 (MQ=255)
                                                                                                                                               ccTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGGCGCTGATGGCGATTAAGATCGACAACATGCGCAACATGGTGCTGAAAGTGGCATGGCAAGCCg       <  2:294541/150‑1 (MQ=255)
                                                                                                                                                 tttGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAACACTGGCGCTGATGGCGAGTAAGATCGACCTCCTGCGCAACATGGTGCTGAAAGTGGCATGGCTAGCCGAt     >  2:236113/1‑150 (MQ=255)
                                                                                                                                                    taaGATGCCGCCCGCTTTGCCAACCAACGTATCGCTTTTGGTAAGCCCATTCGGCATAACCAGATGATCCACGAAAAACTGGCGCTGATGGCGATTAAGATCCACAACATGCGCAACATGGTGCTGAAAGTGGCATGGCAAGCCGATCAg  <  1:519857/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CTGCGAAGTCTATCTCGACAACGTGGAGGTTGAAGAGAGCGACATGGTGGGCGAAGAAGGAATGGGTTTCCTCAATGTGATGTACAACTTTGAGATGGAGCGCCTGATCAACGCCGCGCGCAGCACCGGCTTTGCCGAATGCGCCTTTGAAGATGCCGCCCGCTATGCCAACCAACGTATCGCTTTTGGTAAGCCCATTGGTCATAACCAGATGATCCAGGAAAAACTGGCGCTGATGGCGATTAAGATCGACAACATGCGCAACATGGTGCTGAAAGTGGCATGGCAAGCCGATCAG  >  NC_000913/1777819‑1778116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑