| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 126,093 | T→C | 15.9% | A133A (GCT→GCC) | aceF → | pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 126,093 | 0 | T | C | 15.9% | 198.1 / 20.6 | 88 | A133A (GCT→GCC) | aceF | pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2 |
| Reads supporting (aligned to +/- strand): ref base T (36/36); new base C (7/7); total (43/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGA > NC_000913/125944‑126239 | gCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAACTCCTGGTGAAAGTTGGCGAAAAAGTTGAAGCt > 2:251070/1‑150 (MQ=255)gCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCt < 2:367442/150‑1 (MQ=255) aaCCTGCTCAGGCAGAAGATTAGATAGAAGCAGCTGCGGCAGCACGAGCAGCGTCTGAGGTGGCAAAAGACCTTAACGTTCCGGACATCGGCAGCGACGAAGTTGAATTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTg < 2:1109101/149‑1 (MQ=255) cTGCTCAGGCATCAGAGAAGAAACAAGCAGCTCCGGAATCATCACCAGCGGCTGCGGCGGCAAAAGACGTAAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAAc < 1:614293/150‑1 (MQ=255) cTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGCAATCATGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGt > 2:105992/1‑150 (MQ=255) tCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTc > 2:908321/1‑150 (MQ=255) ggCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGAGGTTAACGTTCCGGATATCGGCAGCGACGAAGTAGAAGTGACCGAAATCCTGGTGATAGTTGGCGAGAATGTTGAAGCTGAACCGTCGCt > 2:502761/1‑150 (MQ=255) ggCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCt < 2:539595/150‑1 (MQ=255) ggCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCt < 1:946906/150‑1 (MQ=255) ggCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTAGAAGCTGCACAGgcgcg > 1:159790/1‑149 (MQ=255) ggCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGAACCAGCGGCTGCGGCGGCAAAAAACGTAAACGTTCCGGATATCGGCATCGACTAAGTTGAAGTGAGCGCAAGCGTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGcg > 2:190617/1‑149 (MQ=255) agaagagaagAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATAACGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGAt > 1:1109944/1‑150 (MQ=255) aagaaagcAGCAGCTCCGGGATCAGCACCAGCGGCTGCTGCGGCAAAAGACGTTAACGTTCCGGACATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTa < 1:442945/150‑1 (MQ=38) agaaagaaGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCTGCGACGAAGTTGATGTGACCGAAATGCTGGTGAAAGTTGGCGATAAAGTTGAGGCTGAACAGTCGCTGATCAGCGTAg > 2:294379/1‑150 (MQ=25) gaaagaaGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTACGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCGTGGGGCAAGTAGGCGAGAAAGTTGTAGCTGACCAGACGCTGATCACCGTAGa > 1:1191165/1‑150 (MQ=255) gaagCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGGTGAAGTGACGGAAGTCCTGGTGAAAGTTGGCGAGAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGc > 1:94395/1‑150 (MQ=255) aagCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCg < 1:239134/150‑1 (MQ=14) tCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGc < 2:487892/150‑1 (MQ=255) ccGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGAGAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCt > 1:1197442/1‑150 (MQ=255) agcagcaCCAGCGGCGGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTTAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTAt < 1:1072667/150‑1 (MQ=255) gcagcaCCAGCGGATGCGGCGGCAAAAGACGTTAAGGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACAGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACTGTCGCTGATCTCCGTAGAAGGGGACAAGGCTTCTATg > 1:52888/1‑150 (MQ=255) caCCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAg < 2:553169/150‑1 (MQ=255) ccAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGtt > 1:733127/1‑150 (MQ=255) aGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAg < 2:84847/146‑1 (MQ=255) aGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAg > 1:84847/1‑146 (MQ=255) aGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGGGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTcc < 2:180427/150‑1 (MQ=255) gCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCg < 1:1311365/150‑1 (MQ=255) cGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCgg < 1:1234664/150‑1 (MQ=255) gcggcgGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCGCCg > 1:1274822/1‑150 (MQ=255) gcAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCt > 1:867035/1‑150 (MQ=255) gcAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCt > 1:871128/1‑150 (MQ=255) gcAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACAGAAATCCTGGTGAAAGTTGGCGATAAAGTTGCAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGATCCGGCTCCGTTTGca > 2:762722/1‑149 (MQ=255) cAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTg > 1:1264353/1‑150 (MQ=255) aaacGACGTGAACGTTCCGGATATTGGCAGCGACGAACTTGAAGTTATCTAAATCATTGTGATACTTGGCTATAAATTTGAAGATTAACATTCTCTGATCAACGTAGAAGTAGAGAAGTCTTCTATGGAAGTTCCGGCTCCGTTTGCTgg < 2:14220/150‑1 (MQ=16) aaaGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGc < 2:1108970/150‑1 (MQ=255) aCGTTAACGTTCCGGATATCGGCAGCGCCGAAGATGAAGAGACCGAAATCCTGGTGAAAGTTGGAGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAATGCTTCTATGGAAGTTACGGCTACGTTTGCTGGCACCg > 2:77999/1‑150 (MQ=18) cGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGCAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGt > 1:635061/1‑150 (MQ=255) gTTGCGGATATCGGCAGTGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTTAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGTCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAagag < 2:38331/150‑1 (MQ=255) ttCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAgaga < 2:899386/150‑1 (MQ=255) cccGGAAATCGCCAGCGACTAAGTTTAATTGTCCGAAAACCTGGTTAAAGTTGTCGATAAACTTGTATCTGACCAGTCGATGATCACCCTAGCAGGCGACAATGCATCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGAt < 2:391730/149‑1 (MQ=255) ccGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATc > 1:417580/1‑150 (MQ=255) tatCGGCAGTGTCGAAGGGCAAGGTCCCGATATCCTTGCGAATGTGGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGt < 1:434291/150‑1 (MQ=18) ggCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAAc > 2:1010147/1‑150 (MQ=255) gCAGCGACGAAGTTGAAGTGTCCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACg > 1:696539/1‑150 (MQ=255) gtcggatgaAGTTGAATTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGt < 2:1000965/143‑1 (MQ=255) cgacgaAGTTGAAGTGACCGAAATCCTGGTGAAAGTT‑GCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCAGGCAGCGTGAATGATCTCAAAGTGAACGTTGGt > 2:384682/1‑150 (MQ=18) gacgaAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAACGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGACGTGAATGAGATCAAAGTCAACGTtggg > 2:1072468/1‑149 (MQ=17) acgaAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTg < 1:442239/150‑1 (MQ=255) gaAGGTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGCAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGAc < 1:460989/150‑1 (MQ=255) aaGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACa > 2:863028/1‑150 (MQ=255) aGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCGACGTGAATGAACTGAAAGTCAACGTTGGCGAGaa > 2:804713/1‑150 (MQ=14) ttGAATTGACCGATATCCTGGTCAAAGTTGTCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAg < 1:1072095/150‑1 (MQ=255) ttGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAg < 2:393486/150‑1 (MQ=17) ttGAAATCACCGACATCCTGGTCAAAGTTGGCGAAAAAGTTGAAGCGGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAg < 2:951306/150‑1 (MQ=18) gAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAgtg < 2:1101681/150‑1 (MQ=255) gAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAgtg < 1:908321/150‑1 (MQ=255) gTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCt < 1:337478/150‑1 (MQ=255) gTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCAGGCGACGTGAAGGAGATCAAAGTCAACGTTGGAGAAAAAgtgaaa > 1:327003/1‑147 (MQ=17) tGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTa > 1:214064/1‑150 (MQ=255) tGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTa > 1:250116/1‑150 (MQ=255) ccGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTg > 2:1301545/1‑110 (MQ=14) ccGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTg < 1:1301545/110‑1 (MQ=14) ccGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCTCTGATCACCGTAGAAGGCGACAAAGc > 2:793583/1‑73 (MQ=255) ccGAAATCCTGGTGAAAGTGGGCGATAAAGTTGAAGCTGAACAGTCTCTGATCACCGTAGAAGGCGACAAAGc < 1:793583/73‑1 (MQ=14) cGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCgg > 2:127380/1‑150 (MQ=255) tCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAgg > 2:83164/1‑97 (MQ=255) tCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCTTCTATGGAAGTTCCGGCGCCGTTTGCAgg < 1:83164/97‑1 (MQ=255) tGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTg > 1:696462/1‑93 (MQ=11) tGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTg < 2:696462/93‑1 (MQ=11) ggTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTa > 2:834752/1‑137 (MQ=255) ggTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTa < 1:834752/137‑1 (MQ=255) gtcaaaGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACg > 2:386735/4‑118 (MQ=17) gtcaaaGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACg < 1:386735/115‑1 (MQ=17) aaGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCTCCGTAGAAGCCTACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGTAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATgg < 2:52888/150‑1 (MQ=255) aaGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATgg < 1:529296/150‑1 (MQ=255) gggCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCGTCTATGGAAGTTCCGGCTCCGTTTGCTGGCAACGTGAAAGAGATCAAAGTGAAAGTTGGTGACAAAGTGTCTACCGGCTAGCTGATTATGGTCtt > 2:784935/2‑150 (MQ=21) aTAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGGCTTCGAAg < 1:304731/150‑1 (MQ=255) aaaGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTc < 1:1005351/150‑1 (MQ=255) aaaGTTGAAGCGGAACAGTCGCTGATGACCGTAGACGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTc < 1:784935/150‑1 (MQ=255) aaaGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTgg < 2:625057/77‑1 (MQ=255) aaaGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTgg > 1:625057/1‑77 (MQ=255) aaGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCTCCGTGAATGAGCTCAAAGTGAACGTGGGTGACAAAGTGTATACCGGCTCGCTGATTATGATCTTCGAAGtag > 2:1179146/1‑148 (MQ=18) gTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGTCGACAACGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCAACGTGAAAGAGCTCACAGTGAACGTGGGTGACAAAGTGGCTACCGGCTCGCTGATTTAGTTCTTCGAAGtagac > 2:990236/1‑146 (MQ=25) tGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCggg < 2:250116/150‑1 (MQ=255) tGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGGCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCggg < 2:214064/150‑1 (MQ=255) aGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGa < 1:886078/150‑1 (MQ=255) aGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAg > 1:444566/1‑89 (MQ=255) aGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAg < 2:444566/89‑1 (MQ=255) aGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTggg < 2:533835/99‑1 (MQ=255) aGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTggg > 1:533835/1‑99 (MQ=255) | GCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGCACCGTGAAAGAGATCAAAGTGAACGTGGGTGACAAAGTGTCTACCGGCTCGCTGATTATGGTCTTCGAAGTCGCGGGTGA > NC_000913/125944‑126239 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |