Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,987,859 G→C 12.3% intergenic (‑101/+14) yecJ ← / ← azuC DUF2766 family protein/acid‑inducible small membrane‑associated protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,987,8590GC12.3% 167.3 / 17.7 65intergenic (‑101/+14)yecJ/azuCDUF2766 family protein/acid‑inducible small membrane‑associated protein
Reads supporting (aligned to +/- strand):  ref base G (28/29);  new base C (3/5);  total (31/34)
Fisher's exact test for biased strand distribution p-value = 7.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.82e-01

CCACATCAGAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCT  >  NC_000913/1987713‑1988006
                                                                                                                                                  |                                                                                                                                                   
ccACATCAGAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGc                                                                                                                                                  <  1:1273597/150‑1 (MQ=255)
 cacaTCAGAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACACCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCa                                                                                                                                                 <  1:424789/150‑1 (MQ=255)
  acaTCAGAAACCAATTCCTGATCTGCATTCAGCGGCTTGGACATCGTAAACTCCTGAAAGCATTTTGAAAGCCGTTATACTAGCGACTGCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAg                                                                                                                                                <  1:24664/150‑1 (MQ=255)
      cAGAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCTCCGGCAGGttt                                                                                                                                            <  1:925846/150‑1 (MQ=255)
         aaaCCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCTCCGTCATCAGGACACAAAAAAACCTGCTCCGGCAGGtttttt                                                                                                                                         <  1:860084/150‑1 (MQ=255)
         aaaCCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCTCCGGCAGGtttttt                                                                                                                                         <  1:1027502/150‑1 (MQ=255)
          acccAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTAATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTg                                                                                                                                        <  1:668383/148‑1 (MQ=255)
           aCCAATTCCTGATCGGCACTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCATGACACAAAAAAACCTGCTCCGGCAGGtttttttg                                                                                                                                       <  1:659288/150‑3 (MQ=255)
              aaTTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCAACCGTCATCAGGACACAAAAAAACCTGCCGGAGCTGCTTTTTTGTTAt                                                                                                                                    >  2:1086951/1‑150 (MQ=255)
                ttCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCg                                                                                                                                  <  2:745614/150‑1 (MQ=255)
                ttCCTGATCGGCATGCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCTCCGGCAGGtttttttgtgtcc                                                                                                                                  <  1:668349/150‑8 (MQ=255)
                 tCCTTATCTGCATTCATCGGCTGGTATATCGTAACCTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGGCACATCCACCGTCATCAGGACACAAAAAAACCTTCCGGAGCAGGTTTTTTGTTATCgg                                                                                                                                 <  2:61759/150‑1 (MQ=255)
                  ccTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTAACATCCACCTACATCAGGACACAAAAAAACCTGCCGGATCAGGTTTTTAGTGATCGGa                                                                                                                                >  2:556691/1‑150 (MQ=255)
                    tGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCTGGAGCAGGTTTTTTGTTATCGGAAc                                                                                                                              >  1:218636/1‑150 (MQ=255)
                    tGATCGGCAATCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAAc                                                                                                                              <  1:1049477/150‑1 (MQ=255)
                     gATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACa                                                                                                                             >  1:1262830/1‑150 (MQ=255)
                     gATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACa                                                                                                                             <  2:28057/150‑1 (MQ=255)
                      aTCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACat                                                                                                                            >  1:546033/1‑150 (MQ=255)
                              tCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTg                                                                                                                    <  1:396198/150‑1 (MQ=255)
                               cAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCCCCGTCATCAGGACACAAAAAAACCTGCCGGCGCAGGTGTTTTGTTATCGGAACATATTGCCTgg                                                                                                                   >  2:588891/1‑150 (MQ=255)
                               cAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTgg                                                                                                                   >  1:590671/1‑150 (MQ=255)
                                 gCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCg                                                                                                                 <  2:289053/150‑1 (MQ=255)
                                     cTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGCCTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTAc                                                                                                             >  1:1108907/1‑150 (MQ=255)
                                        ggACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTc                                                                                                          <  2:894478/150‑1 (MQ=255)
                                          aCATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCtt                                                                                                        >  1:601324/1‑150 (MQ=255)
                                           cATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCttt                                                                                                       <  1:245946/150‑1 (MQ=255)
                                            aTCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTg                                                                                                      >  2:1054444/1‑150 (MQ=255)
                                                 aaaCTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGt                                                                                                 >  2:971529/1‑150 (MQ=255)
                                                     tCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGTTACGTCTTTGAACGTCTTg                                                                                             >  1:418684/1‑150 (MQ=255)
                                                      ccTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGc                                                                                            >  1:1107726/1‑150 (MQ=255)
                                                      ccTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGc                                                                                            <  2:998513/150‑1 (MQ=255)
                                                       cTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCa                                                                                           <  1:10736/150‑1 (MQ=255)
                                                            aGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGt                                                                                      <  1:1044658/150‑1 (MQ=255)
                                                                 tttGATAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGTAACATATTGCATGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTg                                                                                 <  1:853378/150‑1 (MQ=255)
                                                                   tGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGaa                                                                               <  1:746682/150‑1 (MQ=255)
                                                                     aaaGCCGTTATAGTAGCGACTTCACATCTTCAGCGATTGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACa                                                                             <  1:835551/150‑1 (MQ=255)
                                                                      aatccGTTATAGTAGCTACTTCATATCTTCAGCGATAGTCACATGCTCCGTCATAAGGACACAAATAAACCTTCCTGAGCAGGTTTTTTGTTATCGGAACATAATGCGTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACAt                                                                            <  1:179342/147‑1 (MQ=255)
                                                                       aGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATa                                                                           >  2:291705/1‑150 (MQ=255)
                                                                          cGTTATAGTAGCGAATTCACATATTCAGAGATAGTCACATCCACCGTAATCAGTACACAAAAAAACCTGCCTGAGCAGGTTTTTTGTTATCGTAACATATTGCCTGGCGGTACGTCTTTGATCGTCTTGCAATAGTTATTGAACATACtt                                                                        <  1:400402/150‑1 (MQ=255)
                                                                              ataGTAGCGACTTCACATCTTCATCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCa                                                                    <  1:724060/150‑1 (MQ=255)
                                                                              ataGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTTCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCa                                                                    >  2:855422/1‑150 (MQ=255)
                                                                                 gtagCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGa                                                                 >  2:821981/1‑150 (MQ=255)
                                                                                      gACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTg                                                            >  2:103018/1‑150 (MQ=255)
                                                                                         ttCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTgcgc                                                         <  1:971529/150‑1 (MQ=255)
                                                                                         ttCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTgcgc                                                         <  1:399062/150‑1 (MQ=255)
                                                                                               tCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTc                                                   <  2:509997/150‑1 (MQ=255)
                                                                                                             cacaTCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTAACGGAACATATTCCCTGGCGGTACGTCTTTGATCGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTccc                                     >  2:93645/1‑149 (MQ=255)
                                                                                                               caTCCACCGTCATCAGGACCCAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGGACGTCGTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGAc                                   <  1:824484/150‑1 (MQ=255)
                                                                                                               caTCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGAc                                   <  1:884723/150‑1 (MQ=255)
                                                                                                               caTCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGATATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTAATCGCGGCACTCCGAc                                   >  2:496907/1‑150 (MQ=255)
                                                                                                                  ccACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCAt                                >  1:412835/1‑150 (MQ=255)
                                                                                                                   cACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCAtt                               >  2:1032222/1‑150 (MQ=255)
                                                                                                                           tcaGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTCCGGCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATAc                       <  1:1108323/150‑1 (MQ=255)
                                                                                                                              ggACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGTATTTTGCGCAGTTGCATCGCTGCACTCCGACCATTTGTTATACAgg                    <  1:777025/150‑1 (MQ=255)
                                                                                                                                 cacaAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGgtgt                 <  2:418684/150‑1 (MQ=255)
                                                                                                                                    caaaaaaCCTGCTCCGGCAGGTTTTTTGTTATGGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCTGCACTCCGACCATTTGTTATACTGGTGTTAt              >  2:202032/2‑150 (MQ=255)
                                                                                                                                    caaaaaaCCTGCTCCGGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCATTAGTTATTGAACATACTTTTCAGGATTTTGCGCGGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTAt              >  1:31514/2‑150 (MQ=255)
                                                                                                                                     aaaaaaCCTGCTCCGGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTAtt             >  2:910430/1‑150 (MQ=255)
                                                                                                                                     aaaaaaCCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCCCTCCGACCATTTGTTATACAGGTGTTAtt             >  1:1189832/1‑150 (MQ=255)
                                                                                                                                     aaaaaaCCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTAtt             >  2:884006/1‑150 (MQ=255)
                                                                                                                                      aaaaaCCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTg            >  1:577992/1‑150 (MQ=255)
                                                                                                                                        aaaCCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTc          >  1:182117/1‑150 (MQ=255)
                                                                                                                                             tGCCGGAGCAGGTTTTTTGTTATTGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGCCCATTTGTTATACAGGTGTTTTTGTCTTTgc     >  2:342222/1‑150 (MQ=255)
                                                                                                                                              gCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTgcg    >  2:430627/1‑150 (MQ=255)
                                                                                                                                                cGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCt  >  1:920593/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CCACATCAGAAACCAATTCCTGATCGGCATTCAGCGGCTGGGACATCGTAAACTCCTGATAGCATTTTGAAAGCCGTTATAGTAGCGACTTCACATCTTCAGCGATAGTCACATCCACCGTCATCAGGACACAAAAAAACCTGCCGGAGCAGGTTTTTTGTTATCGGAACATATTGCCTGGCGGTACGTCTTTGAACGTCTTGCAATAGTTATTGAACATACTTTTCAGGATTTTGCGCAGTTTCATCGCGGCACTCCGACCATTTGTTATACAGGTGTTATTGTCTTTGCGCT  >  NC_000913/1987713‑1988006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑