Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,992,083	G→C	8.1%	noncoding (35/76 nt)	glyW ←	tRNA‑Gly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,992,083	0	G	C	8.1%	249.9 / 13.7	86	noncoding (35/76 nt)	glyW	tRNA‑Gly
Reads supporting (aligned to +/- strand):  ref base G (29/50);  new base C (4/3);  total (33/53)
Fisher's exact test for biased strand distribution p-value = 4.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.48e-01

GAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAACGATATTTTTCACC  >  NC_000913/1991934‑1992232                                                                                                                                                      |                                                                                                                                                      gAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGATACGAGACTCGAACTCGCGACCCCGACCTTgg                                                                                                                                                       >  1:479526/1‑150 (MQ=255)      ggACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAgg                                                                                                                                                  >  1:790453/1‑150 (MQ=255)         cTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCg                                                                                                                                               <  2:381015/150‑1 (MQ=255)          tAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGt                                                                                                                                              >  2:543035/1‑150 (MQ=255)           aGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTg                                                                                                                                             >  2:984570/1‑150 (MQ=255)           aGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTg                                                                                                                                             >  2:297249/1‑150 (MQ=255)            gACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGCCAAGGTCgggg                                                                                                                                            <  2:713385/150‑4 (MQ=255)             aCGGATTTGCAAGCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCATGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGCCAAGGTCggggt                                                                                                                                           <  2:628637/150‑5 (MQ=255)             aCGGATATGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGCCAAGGTCggggt                                                                                                                                           <  2:344494/150‑5 (MQ=255)                 aTTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTAc                                                                                                                                       <  2:1202679/150‑1 (MQ=255)                  tttGCAATCCGCTACATAACCGCTTAGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTAcc                                                                                                                                      <  1:231833/150‑1 (MQ=255)                    tGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGCCCCCGACCTTGGCAAGGTCGTGCGCTACCaa                                                                                                                                    >  2:1110612/1‑150 (MQ=255)                     gCAATCCGCTACATAACCGCTTTCTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAc                                                                                                                                   <  2:232287/150‑1 (MQ=255)                       aaTCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTg                                                                                                                                 >  2:407723/1‑150 (MQ=255)                            gCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTa                                                                                                                            >  1:8444/1‑150 (MQ=255)                                cATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACTTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTcc                                                                                                                        >  2:1247566/1‑150 (MQ=255)                                cATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTcc                                                                                                                        <  1:40213/150‑1 (MQ=255)                                    aCCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCa                                                                                                                    >  1:465280/1‑150 (MQ=255)                                      cGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCACGCAtt                                                                                                                  >  2:369013/1‑150 (MQ=255)                                       gCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCGTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATtc                                                                                                                 <  2:712818/150‑1 (MQ=255)                                        cTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATtca                                                                                                                <  1:850273/150‑1 (MQ=255)                                        cTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATtca                                                                                                                <  1:297249/150‑1 (MQ=255)                                           tGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAGACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATtcatca                                                                                                             <  1:637873/150‑1 (MQ=255)                                           tGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATtcatca                                                                                                             <  2:995990/150‑1 (MQ=255)                                           tGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCACGCATtcatca                                                                                                             >  1:1247682/1‑150 (MQ=255)                                             ttAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTACACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAg                                                                                                           <  2:343784/150‑1 (MQ=255)                                               aaCGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCa                                                                                                         <  1:291863/150‑1 (MQ=255)                                                    gcCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAg                                                                                                    <  2:47799/150‑1 (MQ=255)                                                     ccAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGt                                                                                                   <  1:549022/150‑1 (MQ=255)                                                            cttcAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACTAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTTTTCCCGCATTCATCAAGCAATCAGTTAATGAc                                                                                            >  2:805370/1‑150 (MQ=255)                                                            cttcAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCAc                                                                                            <  1:661633/150‑1 (MQ=255)                                                             ttcAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTTAACTGGAGCCGGAAACGAGACTCGAACTCGCGACCACGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCACTCAGTTAATCACt                                                                                           <  1:633857/150‑1 (MQ=255)                                                               cAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCAGCATTCATCAAGCAATCAGTTAATCACTTg                                                                                         <  2:338036/150‑1 (MQ=255)                                                                 ggCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGAt                                                                                       >  2:666634/1‑150 (MQ=255)                                                                         aGCCAGACATCCGCTTGACGACGATGTCTTTTAAACTGTAGCGGCAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGTTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTACTCACTTGATGTtattat                                                                               <  2:69935/150‑1 (MQ=25)                                                                          gCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATc                                                                              >  1:318277/1‑150 (MQ=255)                                                                          gCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGATGAGCTCTACCAACTGAGCTAATCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATc                                                                              <  1:866785/150‑1 (MQ=255)                                                                          aaaaGACATCGGCGTCAAGCGGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCGATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTTTc                                                                              >  1:1297514/4‑150 (MQ=21)                                                                           ccAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCg                                                                             <  2:790453/150‑1 (MQ=255)                                                                               aCATCCGCTTGCCGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTg                                                                         <  1:212711/150‑1 (MQ=255)                                                                                cATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTgg                                                                        <  2:596706/150‑1 (MQ=255)                                                                                    cGCTTGACGCCGATGTCTTTTACACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTATACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCaaa                                                                    >  2:919537/1‑149 (MQ=255)                                                                                     gCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATc                                                                   <  1:766347/150‑1 (MQ=255)                                                                                            gCCGATGTCTTTTAAACTGGAGCGTGAAACGAGACTCGAACTCGCGACCCCGTCCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCTAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTgccg                                                            <  2:1232045/150‑1 (MQ=255)                                                                                             ccGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTgccgc                                                           <  2:847574/150‑1 (MQ=255)                                                                                                aTGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGt                                                        <  2:591391/150‑1 (MQ=255)                                                                                                     ttttAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTTTTCCCGCATTCTTCAAGCAAGCGGTTTATCTCTTGATTGTATTATCGTCTGGCCATCAGTGCCGCCGTTCGAt                                                   >  2:935569/1‑150 (MQ=21)                                                                                                      tttAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATg                                                  <  2:609759/150‑1 (MQ=255)                                                                                                       ttATACTGGAGCGGGATATGAGACTCGTTCTCTCGAGCACGACCTTGGCAAGGTCGTGCTCTTCCAAATGAGCTATTCGCGCATTCATCTAGCAATCAGTTAATCTCTTGATTTTATTATGGTCTGGCAATCAGTGCCGCCGTTCGATGc                                                 <  1:867418/150‑1 (MQ=25)                                                                                                          aaCTGGAGCGGGAAACGAGACGGGAACTCGCGACCCCGACCTTCGCAAGGTCGTGCTCTACCAACTGTGCTATTACCGCATTCATCATGCAATGAGTTAATCACTTGTTTTTATTATCGTCTGGGAATCAGTGCCGCCGTTCGATGCGtt                                              <  1:1147747/150‑1 (MQ=25)                                                                                                           aCTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTg                                             <  2:586958/150‑1 (MQ=255)                                                                                                                aGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTc                                        >  1:728571/1‑150 (MQ=255)                                                                                                                 gCGGGAAACGAGACTCGCACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCCTCAAGCAATCAGTTAATCACTTGATTTTATTCTCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCt                                       <  1:201577/150‑1 (MQ=255)                                                                                                                 gCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCt                                       <  2:1285118/150‑1 (MQ=255)                                                                                                                   gggAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCTCCGTTCGCTGCGTTGCATTCTAc                                     >  1:1286341/1‑150 (MQ=255)                                                                                                                   gggAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTAc                                     <  1:369013/150‑1 (MQ=255)                                                                                                                    ggAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCTCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACt                                    >  2:192831/1‑150 (MQ=255)                                                                                                                    ggAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACt                                    >  2:575499/1‑150 (MQ=255)                                                                                                                       aaCGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCAATGCGTTGCATTCTACTTAc                                 <  2:1056413/150‑1 (MQ=255)                                                                                                                         cGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCt                               >  1:334593/1‑150 (MQ=255)                                                                                                                          gagaCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTg                              <  1:806479/150‑1 (MQ=255)                                                                                                                          gagaCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTg                              >  1:497623/1‑150 (MQ=255)                                                                                                                             aCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGgcg                           <  1:492011/150‑1 (MQ=255)                                                                                                                              cTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGgcgc                          >  2:554835/1‑150 (MQ=255)                                                                                                                                  aaCTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATg                      <  2:795097/150‑1 (MQ=255)                                                                                                                                      cgcgACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGCGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTc                  >  2:726198/1‑150 (MQ=255)                                                                                                                                      cgcgACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTc                  <  1:1291593/150‑1 (MQ=255)                                                                                                                                      cgcgACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTc                  >  1:20474/1‑150 (MQ=255)                                                                                                                                      cgcgACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTc                  <  1:728742/150‑1 (MQ=255)                                                                                                                                        cgACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCaa                >  2:886808/1‑150 (MQ=255)                                                                                                                                         gACGCCGACCTTGGCAAGGTCGTGCTGTACCAACTGAGCTATTCCCGAAGTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAAc               <  2:806631/150‑1 (MQ=255)                                                                                                                                         gACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAAc               <  2:787180/150‑1 (MQ=255)                                                                                                                                          agcacGACCTTGCCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAACg              >  2:538030/5‑150 (MQ=255)                                                                                                                                          aCCCCGACCTTGGCAAGGTCGTGCTCTACCATCTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAACg              <  1:717155/150‑1 (MQ=255)                                                                                                                                          aCCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAACg              <  2:1133153/150‑1 (MQ=255)                                                                                                                                           ccccGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTTCATTCTACTTACCTGGCGCGATGAGTCAACGa             <  1:782215/150‑1 (MQ=255)                                                                                                                                           ccccGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAACGa             >  1:899795/1‑150 (MQ=255)                                                                                                                                           ccccGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAACGa             >  2:892059/1‑150 (MQ=255)                                                                                                                                            cccGACCTTGGCAAGGTCGTGCTCTACCACCTGAGCTATACCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAACGat            <  1:815194/150‑1 (MQ=255)                                                                                                                                            cacGACCTTGCCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAACGat            >  2:588624/3‑150 (MQ=255)                                                                                                                                             acgcccTTGCCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAACGata           >  2:86022/5‑150 (MQ=255)                                                                                                                                             acGACCTTGCCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAACGata           >  1:1059005/2‑150 (MQ=255)                                                                                                                                                   ttGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGAGTTTATTATCGTCTGGCACTCAGTGCCGCCGTGCGATGCGTTGCATTCTACTTACCGGGCGCGATGAGTCAACGATATTTTTc     <  1:512877/150‑1 (MQ=255)                                                                                                                                                    tGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAACGATATTTTTca    <  2:479526/150‑1 (MQ=255)                                                                                                                                                    tGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAACGATATTTTTca    <  1:666634/150‑1 (MQ=255)                                                                                                                                                    tGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAACGATATTTTTca    <  1:1247566/150‑1 (MQ=255)                                                                                                                                                    tGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTAGCGCGATGAGTCAACGATATTTTTca    <  2:1179423/150‑1 (MQ=255)                                                                                                                                                      gCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAACGATATTTTTcacc  <  2:465280/150‑1 (MQ=255)                                                                                                                                                      gCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCACTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGATAGATGCGTTGCATTCTACTTACCTGGCGCGATGCGTCCACGATAATGTTcacc  >  1:101891/1‑150 (MQ=255)                                                                                                                                                      |                                                                                                                                                      GAACCGGACTAGACGGATTTGCAATCCGCTACATAACCGCTTTGTTAACGCGCCAAATTCTTCAGGCCTTTCAGCCAGACATCCGCTTGACGCCGATGTCTTTTAAACTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCATTCATCAAGCAATCAGTTAATCACTTGATTTTATTATCGTCTGGCAATCAGTGCCGCCGTTCGATGCGTTGCATTCTACTTACCTGGCGCGATGAGTCAACGATATTTTTCACC  >  NC_000913/1991934‑1992232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑