Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,019,460 G→A 100% V359I (GTC→ATC)  fliK → flagellar hook‑length control protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,019,4600GA100.0% 225.5 / ‑6.7 77V359I (GTC→ATC) fliKflagellar hook‑length control protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (37/39);  minor base C (0/1);  total (37/40)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GAAAGTGGCATTCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGA  >  NC_000913/2019313‑2019605
                                                                                                                                                   |                                                                                                                                                 
gAAAGTGGCATTCACTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCAtc                                                                                                                                                 <  1:226292/150‑1 (MQ=255)
   aGTGGCATTCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCAGTTCCCATatct                                                                                                                                              >  1:853876/1‑150 (MQ=255)
       gCATTCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTAc                                                                                                                                          <  2:1249916/150‑1 (MQ=255)
       gCATTCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAACAAACCATGAACCTCTGGCGGGGGAAGCCGACGATACGCTTCCGGTTCCCATCTCTTTAc                                                                                                                                          >  1:256377/1‑150 (MQ=255)
        cATTCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACa                                                                                                                                         >  1:1080961/1‑150 (MQ=255)
           tCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTTGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAgg                                                                                                                                      <  2:993468/150‑1 (MQ=255)
            cAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGAAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAggg                                                                                                                                     <  2:448707/150‑1 (MQ=255)
             aGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGCCGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGc                                                                                                                                    >  1:1297687/1‑150 (MQ=255)
              gTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCg                                                                                                                                   >  1:1300124/1‑150 (MQ=255)
                tAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAAGCTCTGGCGGTGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCgtg                                                                                                                                 >  1:637499/1‑150 (MQ=255)
                tAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCgtg                                                                                                                                 <  1:464240/150‑1 (MQ=255)
                 aGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCCGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCgtgt                                                                                                                                >  2:198075/1‑150 (MQ=255)
                  gggCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACTGCAAACCCTGAACCTCTGGCGGGGGATGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTa                                                                                                                               <  1:584261/150‑1 (MQ=255)
                        aGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGc                                                                                                                         <  2:1090520/150‑1 (MQ=255)
                           aaCATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAAc                                                                                                                      >  2:837495/1‑150 (MQ=255)
                           aaCATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAAc                                                                                                                      <  1:1370/150‑1 (MQ=255)
                           aaCATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAAc                                                                                                                      >  2:715033/1‑150 (MQ=255)
                            aCATCAGTGGAGAAAGCTTTAGGCGTCAGCAGCCGGCCGCTACCAAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTCGCGGGGGAAGACGACGAGACGCTTCCGGCTCCCAGCTCTTTACAAGGGCGTGTAACAGGCAACa                                                                                                                     <  2:802370/150‑1 (MQ=255)
                             cATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGc                                                                                                                         >  2:960329/1‑145 (MQ=255)
                             cATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGc                                                                                                                         <  1:960329/145‑1 (MQ=255)
                             cATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAg                                                                                                                    >  1:96624/1‑150 (MQ=255)
                                cAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCACCAgcgg                                                                                                                 >  2:237519/1‑150 (MQ=255)
                                 aGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCACCGCACAGCAAGCCATGAACCTCTGGCGGGGGAAGACGCCGATACGCGTCCGGTTCCCATCTCTTTACAAGGGCGTGTCACAGGCAACggcggc                                                                                                                >  2:321293/1‑150 (MQ=255)
                                      cGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGa                                                                                                           >  2:886816/1‑150 (MQ=255)
                                      cGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGa                                                                                                           >  2:371939/1‑150 (MQ=255)
                                       gAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGat                                                                                                          >  2:1135121/1‑150 (MQ=255)
                                         aaGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTCACAGGCAACAGCGGCGTTGatat                                                                                                        <  1:856121/150‑1 (MQ=255)
                                           gCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAttt                                                                                                      >  2:542211/1‑150 (MQ=255)
                                            cTTTAGTGGTCAGCAGCAGGCCGCTTCGCAGCAACAGCAAAGCCAACGCACAGCTAACCATGAACCGCTGGCGGGGGATGCCGACGATACGCGTCCGGTTCCCATCTCTTTACAAGGGCGTGGTTCAGGCTACAGCTGCGTTGATAgttt                                                                                                     >  1:872201/1‑150 (MQ=255)
                                            cTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCATACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGGGTAACAGGCAACAGCGGCGTTGATAtttt                                                                                                     >  2:445804/1‑150 (MQ=255)
                                            cTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAtttt                                                                                                     >  2:1280281/1‑150 (MQ=255)
                                                    gTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAAc                                                                                             >  2:23560/1‑150 (MQ=255)
                                                      cagcagcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGACCCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGt                                                                                           >  1:38408/1‑150 (MQ=255)
                                                           gcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGCACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTGCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATCTTTTCGCCTAACGTCagag                                                                                      >  1:25412/1‑150 (MQ=255)
                                                            cagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATCAACCTCTGGCGGGGAAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAgg                                                                                     <  1:1192992/150‑1 (MQ=255)
                                                              ggCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATCCGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTa                                                                                   >  1:952325/1‑150 (MQ=255)
                                                                ccGATTCCAAGCAACATCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGc                                                                                 <  2:510238/150‑1 (MQ=255)
                                                                   cTTCCCAGAAACAGAAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCAcc                                                                              <  1:208766/150‑1 (MQ=255)
                                                                     tCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGt                                                                            <  1:727904/150‑1 (MQ=255)
                                                                     tCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGt                                                                            <  1:474749/150‑1 (MQ=255)
                                                                      cccAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTATGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCGCTTTACACGGGCGTGTAACAGGCAACACCGGCGTTGAAATTTTCGCCTAACGTCAGAGGTAGCCCCGTa                                                                           >  2:1020282/1‑150 (MQ=255)
                                                                         agcaacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATc                                                                        >  1:468034/1‑150 (MQ=255)
                                                                         agcaacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATc                                                                        <  1:222140/150‑1 (MQ=255)
                                                                                      ccAACGCACGGCAAACCATACAGCTCTGGCGGGGGAAGACGACGATACGCGTCCGGTACCCATCTCATGACCACGGCGTCTAACCGCCAACAGCGGCGTGGCAATTTTCGCCTTACCGCAGAGGTCGCACCGTAATCCGCGTCTATTccc                                                           >  1:209839/1‑150 (MQ=255)
                                                                                      ccAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTccc                                                           >  1:857737/1‑150 (MQ=255)
                                                                                      ccAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTccc                                                           <  2:1308605/150‑1 (MQ=255)
                                                                                       cAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTACGGTTCCCATCTCTTTTCAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCCGAGGTTGCACCGTAATCCGCGACTTTTcccc                                                          >  1:1053838/1‑150 (MQ=255)
                                                                                                gCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTttgttg                                                 <  1:942414/150‑1 (MQ=255)
                                                                                                gCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTttgttg                                                 <  2:416843/150‑1 (MQ=255)
                                                                                                 cAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTgc                                                <  2:1262551/150‑1 (MQ=255)
                                                                                                    aCCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCt                                             <  2:486899/150‑1 (MQ=255)
                                                                                                     ccATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTc                                            <  1:698509/150‑1 (MQ=255)
                                                                                                       aTGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCaa                                          >  1:837984/1‑150 (MQ=255)
                                                                                                        tGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAg                                         <  2:709171/150‑1 (MQ=255)
                                                                                                          aaCCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCGTTCCAAGGGCGGGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGAc                                       <  1:151087/150‑1 (MQ=255)
                                                                                                            ccTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGc                                     <  1:1285390/150‑1 (MQ=255)
                                                                                                              tctGGCGGGGGAAGACGCCGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGAGATTTTCGCCTATCGTCAGAGGTTGCACCGTAATCCGCGTCGTTTCACCGCTTTGGTGCGCTCAAGTCGccg                                   >  2:1078253/1‑148 (MQ=255)
                                                                                                                   cGGTGGAAGACGACGATACTCTTCCTGTTCCCATCTCTTTACAAGGGCGTGTAACCGGGTACAGAGGCGTTGATATTTTCTCCTAACGGCTGAGGGAGCACCGTAATCCGGGCATTTTACCCGCTTTTTTGCGCTCAGGAGGCAGGCTaa                              >  2:224506/1‑150 (MQ=255)
                                                                                                                   cGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATaa                              >  1:380106/1‑150 (MQ=255)
                                                                                                                    gtgggAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACATGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAAt                             <  2:637499/148‑1 (MQ=255)
                                                                                                                       ggAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAg                          <  1:595312/150‑1 (MQ=255)
                                                                                                                       ggAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAg                          <  1:600844/150‑1 (MQ=255)
                                                                                                                         aaGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGcc                        <  1:256520/150‑1 (MQ=255)
                                                                                                                         aaGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGcc                        <  1:274297/150‑1 (MQ=255)
                                                                                                                          aGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGCAGCAGGATAATTAGCCg                       >  2:958807/1‑150 (MQ=255)
                                                                                                                             cgccgaTACGCTTCCGCTTCCCATCTCTTAACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATa                    <  1:198075/147‑1 (MQ=255)
                                                                                                                             cgacgaTACGCTTCCGGTTACCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCGTTGTTGCGCTCAAGACCCAGGATAATTAGCCGaga                    >  1:817664/1‑148 (MQ=255)
                                                                                                                                   tACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGt              <  2:736623/150‑1 (MQ=255)
                                                                                                                                   tACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGt              <  2:264540/150‑1 (MQ=255)
                                                                                                                                      gCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGc           <  2:1118134/150‑1 (MQ=255)
                                                                                                                                       cTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCg          <  2:896815/150‑1 (MQ=255)
                                                                                                                                       cTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCg          >  2:1258384/1‑150 (MQ=255)
                                                                                                                                         tCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCCGAGGTAGCACCGTAATCCGCGTCCTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGac        <  1:768750/150‑1 (MQ=255)
                                                                                                                                         tCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGac        <  2:976311/150‑1 (MQ=255)
                                                                                                                                           cGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGacac      >  2:735101/1‑150 (MQ=255)
                                                                                                                                             gTTCCCCTCTCTTTACAAGCGCGGGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGAAAATTAGCCGAAAAGCAGTAGCGACACAg    <  1:704977/150‑1 (MQ=255)
                                                                                                                                               tCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGa  <  2:843373/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GAAAGTGGCATTCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGA  >  NC_000913/2019313‑2019605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: