Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,024,611 T→A 6.2% intergenic (+20/+24) rcsA → / ← dsrB transcriptional regulator of colanic acid capsular biosynthesis/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,024,6110TA 6.2% 213.8 / 2.2 74intergenic (+20/+24)rcsA/dsrBtranscriptional regulator of colanic acid capsular biosynthesis/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (43/26);  new base A (2/3);  total (45/29)
Fisher's exact test for biased strand distribution p-value = 3.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGC  >  NC_000913/2024462‑2024757
                                                                                                                                                     |                                                                                                                                                  
aGCCAAGACCTTTTCATCGCATAAAGGTAATATTAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTc                                                                                                                                                   <  1:232005/150‑1 (MQ=255)
   cAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTccc                                                                                                                                                 >  2:384024/1‑150 (MQ=255)
          gTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTc                                                                                                                                          >  2:1058253/1‑150 (MQ=255)
            ttCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGGAAACCACCAGTCAg                                                                                                                                        <  2:1130366/150‑1 (MQ=255)
             tCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAgg                                                                                                                                       >  2:1163490/1‑150 (MQ=255)
               aTCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCt                                                                                                                                     >  1:142469/1‑150 (MQ=255)
                tCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGGAAACCACCAGTCAgaatg                                                                                                                                    <  2:552482/150‑5 (MQ=255)
                 cGCATAAAGGTAATATTAAACGTAAGATCAAAACTCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATTGTATTTTTGTCAACATGCGCTAACACATGCTGACTGGTGGGAAACCACCAGTCAgaatgt                                                                                                                                   <  1:672078/150‑6 (MQ=255)
                  gCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGaa                                                                                                                                  >  2:262678/1‑150 (MQ=255)
                   cATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTATTGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGATGAAt                                                                                                                                 >  2:1109546/1‑150 (MQ=255)
                   cATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTTTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCAACAAGTCAGGCTGAAt                                                                                                                                 >  2:632352/1‑150 (MQ=255)
                    aTAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATa                                                                                                                                >  1:445524/1‑150 (MQ=255)
                     tAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCTTGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCACACCAGTCAGGCTGAATaa                                                                                                                               >  1:736766/1‑150 (MQ=255)
                     tAAAGGTAATATTAAACGTAAGATCAAAACACATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATaa                                                                                                                               >  2:764817/1‑150 (MQ=255)
                      aaaGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACGAGTCAGGCTGAAAAAg                                                                                                                              <  2:1146371/150‑1 (MQ=255)
                      aaaGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAg                                                                                                                              <  1:504322/150‑1 (MQ=255)
                        aGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCATGCTGAATAAGAt                                                                                                                            >  1:1129595/1‑150 (MQ=255)
                           tAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCAACCAGTCAGGCTGAATAAGATTAc                                                                                                                         >  2:1135652/1‑150 (MQ=255)
                               atTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTg                                                                                                                     <  1:1163490/150‑1 (MQ=255)
                               atTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTg                                                                                                                     <  1:379047/150‑1 (MQ=255)
                                  aaaCGTTAGATCAAAACGCATAAGAAACAGGTTATCTACAATGTCGTCCGCCTGAGGGATATTGTGAGTAATGGGATTTTTGTCAACATGCGCAACCACCTTCTGACTGGTGGTTTCCCCCCAGGCCGGCTGCATAAGATTACTATGCtt                                                                                                                  >  2:774980/1‑150 (MQ=255)
                                   aaCGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCttt                                                                                                                 <  2:711459/150‑1 (MQ=255)
                                     cGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCACCATGCGCTACCACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGATTAAGATTACTCTGCtttgt                                                                                                               >  1:622966/1‑148 (MQ=255)
                                      gTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTtctc                                                                                                              <  1:375099/150‑1 (MQ=255)
                                        aaGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCca                                                                                                            <  2:473159/150‑1 (MQ=255)
                                             cAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAg                                                                                                       >  1:709596/1‑150 (MQ=255)
                                             cAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAg                                                                                                       <  1:1127742/150‑1 (MQ=255)
                                              aaaaCGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGa                                                                                                      <  1:280144/150‑1 (MQ=255)
                                                  cGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATAcc                                                                                                  <  1:262678/150‑1 (MQ=255)
                                                    cATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGt                                                                                                >  2:1217841/1‑150 (MQ=255)
                                                    cATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGt                                                                                                >  1:870834/1‑150 (MQ=255)
                                                     ataataAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTc                                                                                               >  1:892428/1‑150 (MQ=255)
                                                      taataaACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTcc                                                                                              >  1:1039663/1‑150 (MQ=255)
                                                       aataaACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCt                                                                                             <  2:1257275/150‑1 (MQ=255)
                                                        ataaACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCCGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTg                                                                                            >  1:32313/1‑150 (MQ=255)
                                                           aaCAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATg                                                                                         >  2:1066251/1‑150 (MQ=255)
                                                               ggTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTACCACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGCTGCCCt                                                                                     >  1:1036390/1‑150 (MQ=255)
                                                               ggTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCt                                                                                     >  1:747861/1‑150 (MQ=255)
                                                               ggTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCt                                                                                     >  1:685019/1‑150 (MQ=255)
                                                                  tATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTTGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACAGTACTGCTGCCCTGCt                                                                                  >  2:826692/1‑150 (MQ=255)
                                                                  tATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCt                                                                                  >  2:605994/1‑150 (MQ=255)
                                                                    tCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTc                                                                                <  1:593003/150‑1 (MQ=255)
                                                                            gtcgtcCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGa                                                                        <  2:460777/150‑1 (MQ=255)
                                                                            gtcgtcCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTACCCCCCAGACAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGACATGATGCCCTGCTTCATTAAAGa                                                                        >  2:1085943/1‑150 (MQ=255)
                                                                              cgtcCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCa                                                                               >  2:755888/1‑141 (MQ=255)
                                                                              cgtcCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCa                                                                               <  1:755888/141‑1 (MQ=255)
                                                                                             tAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCggg                                                       <  2:464235/150‑1 (MQ=255)
                                                                                                 gtgACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGt                                                   >  1:781345/1‑150 (MQ=255)
                                                                                                     cTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCCCCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTc                                               >  2:562370/1‑150 (MQ=255)
                                                                                                     cTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACAGATGCCGAGCGGGTAGTCTTcc                                              >  1:497697/1‑150 (MQ=255)
                                                                                                         tGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGc                                           >  1:452921/1‑150 (MQ=255)
                                                                                                              tttttGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAAc                                      <  1:1191668/150‑1 (MQ=255)
                                                                                                               ttttGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAAcc                                     <  2:759348/150‑1 (MQ=255)
                                                                                                               ttttGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAAcc                                     <  2:484139/150‑1 (MQ=255)
                                                                                                                tttGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCa                                    >  2:347438/1‑150 (MQ=255)
                                                                                                                   gTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGt                                 >  2:725694/1‑150 (MQ=255)
                                                                                                                    tCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTa                                <  2:1136595/150‑1 (MQ=255)
                                                                                                                    tCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTa                                <  1:143136/150‑1 (MQ=255)
                                                                                                                         aTGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCGTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATtg                           <  2:830356/150‑1 (MQ=255)
                                                                                                                         aTGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATtg                           <  1:764817/150‑1 (MQ=255)
                                                                                                                          tGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCGCAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGACACCAGGTACATtgt                          >  1:17936/1‑150 (MQ=255)
                                                                                                                          tGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATtgt                          <  2:1042168/150‑1 (MQ=255)
                                                                                                                                aaCACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTc                    >  1:249574/1‑150 (MQ=255)
                                                                                                                                 acacATTCTGACTGGTGGTTTCCCACCAGTCAGAATGAATAAAAATACTATGAATACTAAACAAAAAAAAAAAACACATGCCCTGCTTCATTTAAGAACCAGATTCCGAGCGGGTAGTCTTCAAGCGAAGCCAGGGACATTGTGCCTTCa                   >  2:74645/1‑150 (MQ=255)
                                                                                                                                        cTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAAct            >  2:296461/1‑150 (MQ=255)
                                                                                                                                         tGACTGGTGGGAAACCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATTCCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAACCCAGGTACCTTGTGCCTTCACTAAActc           >  2:967123/1‑150 (MQ=255)
                                                                                                                                          gACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAActcc          >  2:15681/1‑150 (MQ=255)
                                                                                                                                          gACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAActcc          >  1:929834/1‑150 (MQ=255)
                                                                                                                                           aCTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGCTGCCCTGCTGCAGTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCCGGTACATTGTGACGTCAGTAAActccg         >  1:20855/1‑149 (MQ=255)
                                                                                                                                           aCTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAActcct         <  2:870834/150‑1 (MQ=255)
                                                                                                                                            cTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAActcctc        <  1:1058253/150‑1 (MQ=255)
                                                                                                                                              ggtggtTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCGTTCACTACActcctcca      >  2:903962/1‑148 (MQ=255)
                                                                                                                                                 ggtTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTg   >  2:904240/1‑150 (MQ=255)
                                                                                                                                                  gtTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGc  >  2:1018608/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
AGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGCTTCATTAAAGAACCAGATGCCGAGCGGGTAGTCTTCCAGCGAAACCAGGTACATTGTGCCTTCACTAAACTCCTCAACTGC  >  NC_000913/2024462‑2024757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: