Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,197,658 T→A 5.8% pseudogene (1185/2847 nt) yehH → DUF4132 family pseudogene; defective yehI paralog; molybdate metabolism regulator, first fragment; molybdate metabolism regulator, second fragment 2; yehI' molR mutations have phenotypes and the N‑terminal molR fragment of yehI' may be expressed and may have retained or evolved MolR function

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,197,6580TA 5.8% 211.9 / 2.1 81pseudogene (1185/2847 nt)yehHDUF4132 family pseudogene; defective yehI paralog; molybdate metabolism regulator, first fragment; molybdate metabolism regulator, second fragment 2; yehI' molR mutations have phenotypes and the N‑terminal molR fragment of yehI' may be expressed and may have retained or evolved MolR function
Reads supporting (aligned to +/- strand):  ref base T (40/36);  new base A (2/3);  total (42/39)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.34e-01

CTGATGAGCACATTCTTGCCTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGTATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCTTATACGTGTAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGG  >  NC_000913/2197510‑2197806
                                                                                                                                                    |                                                                                                                                                    
cTGATGAGCACATTCTTGCCTCATTTGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGTATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTg                                                                                                                                                     >  2:543252/1‑150 (MQ=255)
      aGCACATTCTTGCCTCATTAGAAAAATATCACGAACCATATGCCATTTTTGTTGATTACTATTGTCGATCGATATGGTCAGACACCGTATTACATGAGCAAGGTGTTACAGCCCTGCACCGATTTGCTCCCTATGCAGCAAGTGacaact                                                                                                                                               >  1:1136114/1‑150 (MQ=255)
       gCACATTCTTGCCTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGTATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTg                                                                                                                                              >  1:598566/1‑150 (MQ=255)
        cacaTTCTTGCCTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGTATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTgc                                                                                                                                             <  1:534133/150‑1 (MQ=255)
              cTTGCCTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGTATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATTCCGCAAGTGACTACTGCGCCGAt                                                                                                                                       >  2:786873/1‑150 (MQ=255)
                  ccTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGTATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATgtgt                                                                                                                                   >  2:454321/1‑150 (MQ=255)
                  ccTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGTATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATgtgt                                                                                                                                   >  2:1290499/1‑150 (MQ=255)
                  ccTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGTATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCAGATgtgt                                                                                                                                   >  2:748885/1‑150 (MQ=255)
                  ccTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGTATTACAGGAGCAAGGTGTTGCAGCCATGCCCCGATTTGCTCCCTATGCAGCAAGTGACTACTGCGCCGATgtgt                                                                                                                                   >  1:623791/1‑150 (MQ=255)
                    tCATTAGAATAATATAACGAACCATATGCAATTTTAGATGATTACTATAGTCGTGCGATATGGTCAGCCACCGTATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGAGTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTg                                                                                                                                 <  2:79902/150‑1 (MQ=255)
                      aTTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGTATTACAGGAGCAAGGGGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCg                                                                                                                               <  2:268779/150‑1 (MQ=255)
                        tAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGTATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTc                                                                                                                             >  2:730464/1‑150 (MQ=255)
                             aaaTATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGTATTACAGTAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATatc                                                                                                                        <  2:543891/150‑1 (MQ=255)
                                tatCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGTATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATatcaat                                                                                                                     >  1:872021/1‑150 (MQ=255)
                                 atCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGTTCAGCCACCGTATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATatcaatc                                                                                                                    <  1:854188/150‑1 (MQ=255)
                                             atGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGTATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCAt                                                                                                        <  2:199898/150‑1 (MQ=255)
                                              tGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGTATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGAGGTGTTGCGTCACATCAATCTTCCGTTCGCagt                                                                                                       >  2:688722/1‑148 (MQ=255)
                                                ccATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGTATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGa                                                                                                     >  2:463063/1‑150 (MQ=255)
                                                   tttttGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGAATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGacac                                                                                                  >  2:881056/1‑150 (MQ=255)
                                                    ttttGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGTATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACt                                                                                                 <  1:356371/150‑1 (MQ=255)
                                                      ttGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGTATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCTATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGc                                                                                               <  2:88056/150‑1 (MQ=255)
                                                        gatgatTACTATTGTGGTGCGATATGGTCAGCCACCGTATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCtt                                                                                             >  2:425036/1‑150 (MQ=255)
                                                         atgatTACTATTGTGGTGCGATATGGTCAGCCACCGTATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCTta                                                                                            >  2:1297183/1‑150 (MQ=255)
                                                         atgatTACTATTGTGGTGCGATATGGTCAGCCACCGTATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCTta                                                                                            <  2:691872/150‑1 (MQ=255)
                                                           gatTACTATTGTGGTGCGATATGGTCAGCCACCGTATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCTtata                                                                                          <  1:1108089/150‑1 (MQ=255)
                                                             ttACTATTGTGGTGCGATATGGTCAGCCACCGTATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCTTATACg                                                                                        <  2:1199911/150‑1 (MQ=255)
                                                                   ttGTGGTGCGATATGGTCAGCCACCGTATTACAGTAGAAAGGTGTTGCAGCCCTGCCCCGATTTTCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCTCATTGACACTGATTATACGTGTAGc                                                                                  <  1:684628/150‑1 (MQ=255)
                                                                   ttGTGGTGCGATATGGTCAGCCACCGTATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCTTATACGTGTAGc                                                                                  <  2:731533/150‑1 (MQ=255)
                                                                   ttGTGGAGCGATATGGTCAGCCACCGTATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGATCGCATTGACACTGCTTATACGTGTAGc                                                                                  <  2:241734/150‑1 (MQ=255)
                                                                    tgtgGTGCGATATGGTCAGCCACCGTATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCTTATACGTGTAGcc                                                                                 <  2:719930/150‑1 (MQ=255)
                                                                    tgtgGTGCGATATGGTCAGCCACCGTATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCTTATACGTGTAGcc                                                                                 <  2:433914/150‑1 (MQ=255)
                                                                       ggtgCGATATGGTCAGCCACCGTATTACAGGAGGAAGGTGTTGCAGCCCTGCCCCGTGGTGCTCCCTATGCCGCAAGTGACTACTGCGGCGATGTGTTGCGTCATATCCCTCCTCCGTTCGCTTTGGGAGTGCGTATACGTGTAGCCggg                                                                              >  2:334686/1‑150 (MQ=255)
                                                                       ggtgCGATATGGTCAGCCACCGTATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCTTATACGTGTAGCCggg                                                                              >  2:880250/1‑150 (MQ=255)
                                                                       ggtgCGATATGGTCAGCCACCGTATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCTTATACGTGTAGCCggg                                                                              <  1:454321/150‑1 (MQ=255)
                                                                            gATATGGTCAGCCACCGTATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGTGTCATATCAATCATCCGTTCGCATTGACACTGCTTATACGTGTAGCCGGGCAAAc                                                                         <  1:786873/150‑1 (MQ=255)
                                                                                tGGTCAGCCACCGTATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCTCATTGACACTGCTTATACGTGTAGCCGGGCAAACTaaa                                                                     >  2:237221/1‑150 (MQ=255)
                                                                                tGGTCAGCCACCGTATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATACGTTCGCATTGCCACTGATTATACGTGTAGCCGGGCAAACTaaa                                                                     >  1:92719/1‑150 (MQ=255)
                                                                                  gTCAGCCACCGTATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCTTATACGTGTAGCCGGGCAAACTAAACg                                                                   <  1:694165/150‑1 (MQ=255)
                                                                                   tCAGCCACCGTATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCTTATACGTGTAGCCGGGCAAACTAAACGc                                                                  >  2:660880/1‑150 (MQ=255)
                                                                                    cAGCCACCGTATAACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATGTGCTCCCTATGCCTCAAGTGACTACTGCGCCGATGGGTGCCGTCATATCAATCATCAGTTCGCATTGACACTGCTTATACGTGTAGCCGGGCAAACTAAACGCt                                                                 <  2:193786/150‑1 (MQ=255)
                                                                                      gCCACCGTATTACAGCAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCACTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCTTATACGTGTAGCCGGGCAAACTAAACGCTGt                                                               >  2:363713/1‑150 (MQ=255)
                                                                                       ccACCGTATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCTTATACGTGTAGCCGGGCAAACTAAACGCTGTc                                                              >  1:1201064/1‑150 (MQ=255)
                                                                                       ccACCGAATTACAGGCGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGAGACTACTGCGCCGATGTGTGGCGTCATATCAATCATCCGTTCGCATTGACACTGCATATACGTGTAGCCGGGCAAACTAAACGCTGTc                                                              <  2:181255/150‑1 (MQ=255)
                                                                                        cACCGTATTACAGGAGCAAGGTGGTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCTTATACGTGTAGCCGGGCAAACTAAACGCTGTCa                                                             <  2:548699/150‑1 (MQ=255)
                                                                                        cACCGTATTACAGGAGCAAGGGGGTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCTTATACGTGTAGCCGGGCAAACTAAACGCTGTCa                                                             <  2:529122/150‑1 (MQ=255)
                                                                                             tATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCTTATACGTGTAGCCGGGCAAACTAAACGCTGTCACGATc                                                        <  1:537871/150‑1 (MQ=255)
                                                                                                tACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCTTATACGTGTAGCCGGGCAAACTAAACGCTGTCACGATCGGa                                                     <  2:945998/150‑1 (MQ=255)
                                                                                                tACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGAATTGACACTGCTTATACGTGTAGCCGGGCAAACTAAACGCTGTCACGATCggt                                                     >  2:815437/1‑149 (MQ=255)
                                                                                                 aCAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCTTATACGTGTAGCCGGGCAAACTAAACGCTGTCACGATCGGAt                                                    <  1:756866/150‑1 (MQ=255)
                                                                                                  cAGGAGAAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCTTATACGTGTAGCCGGGCAAACTAAACGCTGTCACGATCGGATg                                                   <  2:499870/150‑1 (MQ=255)
                                                                                                    ggAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCTTATACGTGTAGCCGGGCAAACTAAACGCTGTCACGATCGGATGAc                                                 <  1:375729/150‑1 (MQ=255)
                                                                                                    ggAGCAAGGTGTTGCAGCCCTGCCCCGATTTGATCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCTTTGACACTGCTTATACGTGTAGCCGGTCAAACTAAACGCTGACACGATCGGATGAc                                                 >  1:224031/1‑150 (MQ=255)
                                                                                                     gAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGAGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCTTATACGTGTAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACg                                                >  1:1048208/1‑150 (MQ=255)
                                                                                                     gAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGAGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCTTATACGTGTAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACg                                                >  1:1053530/1‑150 (MQ=255)
                                                                                                        cAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCTTATACGTGTAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGaaa                                             >  2:834648/1‑150 (MQ=255)
                                                                                                          aGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCTTATACGTGTAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGAAAGc                                           >  1:270634/1‑150 (MQ=255)
                                                                                                           ggTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGAGCCGATGTGTTCCGTGATATCAATCATACCATCTCTTTGACACTGCTTATACGTGTACACGGGCAAACTACACGCTGACACGATCGCATGACGAAAGcc                                          >  2:384355/1‑150 (MQ=255)
                                                                                                                  cAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGAGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCTTATACGTGTAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATtgctg                                   <  2:1053530/150‑1 (MQ=255)
                                                                                                                  cAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGAGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCTTATACGTGTAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATtgctg                                   <  2:1048208/150‑1 (MQ=255)
                                                                                                                     cccTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCTTATACGTGTAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGt                                >  2:809034/1‑150 (MQ=255)
                                                                                                                        tGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGCTGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCTTATACGTGTAGCCTGGCAAACTAAACGCTGTCACGATCGGATGACGACAGCCATTGCGGCGTTcc                             >  1:671551/1‑150 (MQ=255)
                                                                                                                        tGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCTTATACGTGTAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTcc                             >  1:1202448/1‑150 (MQ=255)
                                                                                                                        tGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCTTATACGTGTAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTcc                             >  2:667778/1‑150 (MQ=255)
                                                                                                                         gCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCTTATACGTGTAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGCAAGCCATTGCTGCGTTccc                            >  1:874089/1‑150 (MQ=255)
                                                                                                                         gCCCCGATTTGCTCCCCATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCTTATACGTGTAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTccc                            >  1:884210/1‑150 (MQ=255)
                                                                                                                          ccccGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCTTATACGTGTAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGACAGCCATTGCTGCGTTCCca                           >  2:1078495/1‑150 (MQ=255)
                                                                                                                             cGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCTTATACGTGTAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACAt                        >  2:1156379/1‑150 (MQ=255)
                                                                                                                                tttGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCTTATACGTGTAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATgca                     <  2:268655/150‑1 (MQ=255)
                                                                                                                                tttGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCTTATACGTGTAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATgca                     <  2:147715/150‑1 (MQ=255)
                                                                                                                                 ttGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCTTATACGTGTAGCCGGGTAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATgcag                    <  1:748885/150‑1 (MQ=255)
                                                                                                                                     tCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCCTATCAATCATCCGTTCGCATTGACACTGCTTATACGTGTAGCCGGGCAACCTAAACGCTGTCACGATCGGATGAAGAAAGCCATTGCTGCGGTCCCACATGCCGCAAt                >  2:917480/1‑150 (MQ=255)
                                                                                                                                     tCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCTTATACGTGTAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAAt                <  1:492660/150‑1 (MQ=255)
                                                                                                                                     tCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCTTATACGTGTAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAAt                <  1:730464/150‑1 (MQ=255)
                                                                                                                                      cccTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCTTATACGTGTAGCTGGGCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATg               <  1:1135513/150‑1 (MQ=255)
                                                                                                                                      cccTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCTTATACGTGTAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATg               <  2:1119925/150‑1 (MQ=255)
                                                                                                                                        cTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCTTATACGTGTAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATggc             <  2:346281/150‑1 (MQ=255)
                                                                                                                                            cccGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCTTATACGTGTAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCa         >  1:716393/2‑150 (MQ=255)
                                                                                                                                              cGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCTTATACGTGTAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACt       >  1:297687/1‑150 (MQ=255)
                                                                                                                                                  aGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCTTATACGTGTAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGACAGCCATTGCTGCGTTCCCACCTGCAgcactggcggcactggcg   >  2:1196823/1‑150 (MQ=255)
                                                                                                                                                   gTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCTGTTCGCATTGACACTGCTTATACGTGTAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACgg  >  2:2400/1‑150 (MQ=255)
                                                                                                                                                   gTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCTTATACGTGTAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACgg  <  1:1046047/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CTGATGAGCACATTCTTGCCTCATTAGAAAAATATCACGAACCATATGCCATTTTTGATGATTACTATTGTGGTGCGATATGGTCAGCCACCGTATTACAGGAGCAAGGTGTTGCAGCCCTGCCCCGATTTGCTCCCTATGCCGCAAGTGACTACTGCGCCGATGTGTTGCGTCATATCAATCATCCGTTCGCATTGACACTGCTTATACGTGTAGCCGGGCAAACTAAACGCTGTCACGATCGGATGACGAAAGCCATTGCTGCGTTCCCACATGCAGCAATGGCGGCACTGACGG  >  NC_000913/2197510‑2197806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: