Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,259,686 A→G 6.7% intergenic (‑390/+33) psuK ← / ← fruA pseudouridine kinase/fused fructose‑specific PTS enzymes: IIBcomponent/IIC components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,259,6860AG 6.7% 216.1 / 5.9 74intergenic (‑390/+33)psuK/fruApseudouridine kinase/fused fructose‑specific PTS enzymes: IIBcomponent/IIC components
Reads supporting (aligned to +/- strand):  ref base A (35/34);  new base G (2/3);  total (37/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.88e-01

AAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAG  >  NC_000913/2259537‑2259834
                                                                                                                                                     |                                                                                                                                                    
ctcaaGGGCAGATTATTTTCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTacagga                                                                                                                                                      >  2:925953/4‑150 (MQ=255)
 aaaaGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTacaggac                                                                                                                                                     >  1:1302426/1‑150 (MQ=255)
   aaGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTacaggacag                                                                                                                                                   >  2:453777/1‑150 (MQ=255)
       gCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGaaa                                                                                                                                               <  2:741892/150‑1 (MQ=255)
          gAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAAttt                                                                                                                                            <  1:654770/150‑1 (MQ=255)
           aaaaTAATCTGCCCTTGAGGAAATAACCTGAGTGGTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTc                                                                                                                                           <  2:94661/150‑1 (MQ=255)
               taatCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCCCAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACATGACAGTAAAtttaagcc                                                                                                                                       >  1:643442/1‑145 (MQ=255)
               taatCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGcc                                                                                                                                       <  1:762342/150‑1 (MQ=255)
                 atCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCATTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCt                                                                                                                                     >  1:1081689/1‑150 (MQ=255)
                   cTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATTTTACAGGACAGGAAATTTCTGCCCTGt                                                                                                                                   >  1:193199/1‑150 (MQ=255)
                    tGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGGCAGAAATTTCCTGTCCTGTa                                                                                                                                  <  2:130706/150‑1 (MQ=255)
                     gCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTaa                                                                                                                                 >  1:1142673/1‑150 (MQ=255)
                       ccTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAaca                                                                                                                               <  2:363546/150‑1 (MQ=255)
                        cTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGGCAGAAATTTCCTGTCCTGTAacat                                                                                                                              <  1:600729/150‑2 (MQ=255)
                          tGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTTCATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGGCAGAAATTTCCTGTCCTGTAacattt                                                                                                                            <  2:794324/150‑4 (MQ=39)
                          tGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAacacac                                                                                                                            <  1:495615/150‑1 (MQ=255)
                          tGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAacacac                                                                                                                            >  2:4821/1‑150 (MQ=255)
                          tGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATAACATAAACAAACGGTTCATACTCTTTGCCATCATCAAATTTTACAGGACAGTAAATTAATGCCATTTAacacac                                                                                                                            >  2:302695/1‑150 (MQ=255)
                           gAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGTACAGGAAATTTCTGCCCTGTAACACAcc                                                                                                                           <  2:537990/150‑1 (MQ=255)
                             ggAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACAtt                                                                                                                         >  1:489818/1‑150 (MQ=255)
                             ggAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTAATGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACTAACCGGTAATTCTCTATGCGCTCTTCAAATGTTACAGGACAGGAAATTTATGAACTTTAAAACAACtt                                                                                                                         >  1:1198258/1‑150 (MQ=255)
                                 aTAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTttat                                                                                                                     <  1:709617/150‑1 (MQ=255)
                                    aCCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTAc                                                                                                                  >  2:62348/1‑150 (MQ=255)
                                    aCCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTAc                                                                                                                  <  1:790264/150‑1 (MQ=255)
                                              ttGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCg                                                                                                        <  1:270238/150‑1 (MQ=255)
                                               tgatgaACGTTTGAAGTTTCTACTGAAAGATATAACCATTTAATTTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGc                                                                                                       <  1:173066/150‑1 (MQ=255)
                                               tgatgaACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTTTAACACACCTTTTATTACGCTGCTTTCGc                                                                                                       >  2:28795/1‑150 (MQ=255)
                                               tgatgaACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCATGTAACACACCTTTTATTACGCTGCTTTCGc                                                                                                       >  2:1000799/1‑150 (MQ=255)
                                                gatgaACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCt                                                                                                      >  2:197225/1‑150 (MQ=255)
                                                 atgaACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTATGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCACTGTAACCCACCTTTTATTACGCTGCATTCGCTa                                                                                                     >  1:756062/1‑150 (MQ=255)
                                                  tgaACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTGTCGCTAc                                                                                                    >  2:1117645/1‑150 (MQ=255)
                                                    aaCGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTg                                                                                                  <  1:475596/150‑1 (MQ=255)
                                                         ttGAAGTTTATACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCATTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTcc                                                                                             <  1:207788/150‑1 (MQ=255)
                                                          tGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCa                                                                                            >  1:692880/1‑150 (MQ=255)
                                                                 tCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCgg                                                                                     >  1:93623/1‑150 (MQ=255)
                                                                  cTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTAATACGCTGCTTTCGCTACTGCGTCCACTTCCggc                                                                                    >  2:1143618/1‑149 (MQ=255)
                                                                   tACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTATGCGCTCATCAAATGTTACAGGACAGGAAAATTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGACCACTTCCGGAc                                                                                   >  2:1008460/1‑150 (MQ=255)
                                                                    aCTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCGTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACg                                                                                  >  2:352883/1‑150 (MQ=255)
                                                                     cTGAAAGATAAAAACATTTAAATTCATAAAATTACATTCACAAACCCGTCATTCTCATTGCGCTCATCAAATGTTACAGGACACGAAATATCTGCCCTGTTACACACCTTTTAATACGCTGCTTTCGCTACTGCTTCCACTTCCGGACGt                                                                                 >  2:683429/1‑150 (MQ=255)
                                                                       taaaGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGttt                                                                               <  1:1301827/149‑1 (MQ=255)
                                                                          atataataCCATTTAAATTCATAAAATTACATTCACATACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTATCACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAg                                                                            <  1:1160281/148‑1 (MQ=255)
                                                                            aTAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGa                                                                          >  1:274729/1‑150 (MQ=255)
                                                                               aatccATTTAAATTCATATAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACCGGACAGGAAATTTCTGCCCTGTAAGTCACCTTTTATTACGCTTATTTCGGTACTGCGTCCTGTTCCAGAGGTTTCAGGatgt                                                                       <  2:924724/147‑4 (MQ=255)
                                                                               aaaCCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAgg                                                                       >  1:909931/1‑150 (MQ=255)
                                                                                     tttAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAgg                                                                 <  2:1157699/150‑1 (MQ=255)
                                                                                      ttAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGc                                                                <  2:1027080/150‑1 (MQ=255)
                                                                                       tAAATTCATAAAATTTCATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGcc                                                               >  1:1045442/1‑150 (MQ=255)
                                                                                        aaaTTCTTAAATTTACATTCAAAAAGCGGTGATTCTCTTTGCGCTCATCATATGTTTCAGGACAGGAAATTTCTGCCTTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCa                                                              <  2:641609/150‑1 (MQ=255)
                                                                                         aaTTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCaa                                                             <  1:94160/150‑1 (MQ=255)
                                                                                         aaTTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCaa                                                             >  1:1125394/1‑150 (MQ=255)
                                                                                           ttCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAACTTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAAc                                                           <  1:86317/150‑1 (MQ=255)
                                                                                             cATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAAccc                                                         >  2:358373/1‑150 (MQ=255)
                                                                                                aaaaTTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGcc                                                      <  1:925953/150‑1 (MQ=255)
                                                                                                       cATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCg                                               >  1:797721/1‑150 (MQ=255)
                                                                                                          tCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTAc                                            >  2:583200/1‑150 (MQ=255)
                                                                                                            acaAACCGTTGATTCTCATTGCGCGCATCATATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTAGCTACTGCGTCAACTTGCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCAGCAGCGTAcct                                          <  2:878736/150‑2 (MQ=255)
                                                                                                            acaAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCg                                          >  2:339476/1‑150 (MQ=255)
                                                                                                             caAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCgg                                         <  2:907028/150‑1 (MQ=255)
                                                                                                                 ccGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCaat                                     >  2:1040674/1‑150 (MQ=255)
                                                                                                                   ggTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCaataa                                   <  1:1295800/150‑1 (MQ=255)
                                                                                                                   ggTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGATGCTTTCGCTACTGCGTACACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCCGCTGTCCGGCaataa                                   >  2:811308/1‑150 (MQ=255)
                                                                                                                    gTCATTCTCTTTGCGCTCATGAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCaataat                                  <  2:756062/150‑1 (MQ=255)
                                                                                                                      cATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTg                                <  2:699938/150‑1 (MQ=255)
                                                                                                                        ttCTCTTTGCGCTCATCAAATGTTACAGGACAGGAACTTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCt                              <  1:133264/150‑1 (MQ=255)
                                                                                                                            ctTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATAGCTACCa                          >  2:1028055/1‑150 (MQ=255)
                                                                                                                                     catcaAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCa                 <  1:607605/150‑1 (MQ=255)
                                                                                                                                     catcaAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCa                 >  2:1279923/1‑150 (MQ=255)
                                                                                                                                     catcaAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCa                 >  2:1268137/1‑150 (MQ=255)
                                                                                                                                         gtgtGTTACAGGGCAGAAATTTCCTGTCCTGTAACATACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATAc             >  1:181464/4‑150 (MQ=39)
                                                                                                                                          aaTGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACTCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATAcc            <  1:674449/150‑1 (MQ=255)
                                                                                                                                          aaTGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACTCAATAcc            <  1:1127632/150‑1 (MQ=255)
                                                                                                                                          aaTGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATAcc            <  1:318691/150‑1 (MQ=255)
                                                                                                                                             gTTACAGGGCAGAAATTTCCTGTCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTTCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGc         >  2:639377/1‑150 (MQ=255)
                                                                                                                                             gTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCGCTTCCGGACGTTTCAGGAAGGCATAGGCCACACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTACCCCAATACCGGc         >  1:886805/1‑150 (MQ=255)
                                                                                                                                             gTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCTGACTTTTCAGGAAGGCATAGGCCAAACACGACACCAGCGTAACGGCAATTAATGCTACCAGGTAACCAAATACCGGc         >  1:887418/1‑150 (MQ=255)
                                                                                                                                              ttACATGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCg        <  1:447473/150‑1 (MQ=255)
                                                                                                                                              ttACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCg        <  1:250663/150‑1 (MQ=255)
                                                                                                                                                  aggacaggaAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACAAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAAt    >  1:1300976/1‑150 (MQ=255)
                                                                                                                                                   ggacaggaAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATa   >  1:477934/1‑150 (MQ=255)
                                                                                                                                                   ggacaggaAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATa   >  2:262939/1‑150 (MQ=255)
                                                                                                                                                    gacaggaTATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTATCCCAATACCGGCGTAATAg  <  1:302695/150‑1 (MQ=255)
                                                                                                                                                    gacaggaAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAg  <  2:909931/150‑1 (MQ=255)
                                                                                                                                                    gacaggaAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAg  <  1:4821/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAG  >  NC_000913/2259537‑2259834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: