Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,259,690 G→A 6.9% intergenic (‑394/+29) psuK ← / ← fruA pseudouridine kinase/fused fructose‑specific PTS enzymes: IIBcomponent/IIC components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,259,6900GA 6.9% 202.7 / 4.2 74intergenic (‑394/+29)psuK/fruApseudouridine kinase/fused fructose‑specific PTS enzymes: IIBcomponent/IIC components
Reads supporting (aligned to +/- strand):  ref base G (33/34);  new base A (2/3);  total (37/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-01

GCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGCC  >  NC_000913/2259544‑2259838
                                                                                                                                                  |                                                                                                                                                    
gCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGaaa                                                                                                                                                   <  2:741892/150‑1 (MQ=255)
   gAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAAttt                                                                                                                                                <  1:654770/150‑1 (MQ=255)
    aaaaTAATCTGCCCTTGAGGAAATAACCTGAGTGGTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTc                                                                                                                                               <  2:94661/150‑1 (MQ=255)
        taatCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCCCAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACATGACAGTAAAtttaagcc                                                                                                                                           >  1:643442/1‑145 (MQ=255)
        taatCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGcc                                                                                                                                           <  1:762342/150‑1 (MQ=255)
          atCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCATTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCt                                                                                                                                         >  1:1081689/1‑150 (MQ=255)
            cTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATTTTACAGGACAGGAAATTTCTGCCCTGt                                                                                                                                       >  1:193199/1‑150 (MQ=255)
             tGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGGCAGAAATTTCCTGTCCTGTa                                                                                                                                      <  2:130706/150‑1 (MQ=255)
              gCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTaa                                                                                                                                     >  1:1142673/1‑150 (MQ=255)
                ccTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAaca                                                                                                                                   <  2:363546/150‑1 (MQ=255)
                 cTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGGCAGAAATTTCCTGTCCTGTAacat                                                                                                                                  <  1:600729/150‑2 (MQ=255)
                   tGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTTCATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGGCAGAAATTTCCTGTCCTGTAacattt                                                                                                                                <  2:794324/150‑4 (MQ=39)
                   tGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAacacac                                                                                                                                <  1:495615/150‑1 (MQ=255)
                   tGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAacacac                                                                                                                                >  2:4821/1‑150 (MQ=255)
                   tGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATAACATAAACAAACGGTTCATACTCTTTGCCATCATCAAATTTTACAGGACAGTAAATTAATGCCATTTAacacac                                                                                                                                >  2:302695/1‑150 (MQ=255)
                    gAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGTACAGGAAATTTCTGCCCTGTAACACAcc                                                                                                                               <  2:537990/150‑1 (MQ=255)
                      ggAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACAtt                                                                                                                             >  1:489818/1‑150 (MQ=255)
                      ggAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTAATGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACTAACCGGTAATTCTCTATGCGCTCTTCAAATGTTACAGGACAGGAAATTTATGAACTTTAAAACAACtt                                                                                                                             >  1:1198258/1‑150 (MQ=255)
                          aTAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTttat                                                                                                                         <  1:709617/150‑1 (MQ=255)
                             aCCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTAc                                                                                                                      <  1:790264/150‑1 (MQ=255)
                             aCCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTAc                                                                                                                      >  2:62348/1‑150 (MQ=255)
                                       ttGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCg                                                                                                            <  1:270238/150‑1 (MQ=255)
                                        tgatgaACGTTTGAAGTTTCTACTGAAAGATATAACCATTTAATTTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGc                                                                                                           <  1:173066/150‑1 (MQ=255)
                                        tgatgaACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTTTAACACACCTTTTATTACGCTGCTTTCGc                                                                                                           >  2:28795/1‑150 (MQ=255)
                                        tgatgaACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCATGTAACACACCTTTTATTACGCTGCTTTCGc                                                                                                           >  2:1000799/1‑150 (MQ=255)
                                         gatgaACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCt                                                                                                          >  2:197225/1‑150 (MQ=255)
                                          atgaACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTATGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCACTGTAACCCACCTTTTATTACGCTGCATTCGCTa                                                                                                         >  1:756062/1‑150 (MQ=255)
                                           tgaACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTGTCGCTAc                                                                                                        >  2:1117645/1‑150 (MQ=255)
                                             aaCGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTg                                                                                                      <  1:475596/150‑1 (MQ=255)
                                                  ttGAAGTTTATACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCATTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTcc                                                                                                 <  1:207788/150‑1 (MQ=255)
                                                   tGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCa                                                                                                >  1:692880/1‑150 (MQ=255)
                                                          tCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCgg                                                                                         >  1:93623/1‑150 (MQ=255)
                                                           cTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTAATACGCTGCTTTCGCTACTGCGTCCACTTCCggc                                                                                        >  2:1143618/1‑149 (MQ=255)
                                                            tACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTATGCGCTCATCAAATGTTACAGGACAGGAAAATTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGACCACTTCCGGAc                                                                                       >  2:1008460/1‑150 (MQ=255)
                                                             aCTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCGTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACg                                                                                      >  2:352883/1‑150 (MQ=255)
                                                              cTGAAAGATAAAAACATTTAAATTCATAAAATTACATTCACAAACCCGTCATTCTCATTGCGCTCATCAAATGTTACAGGACACGAAATATCTGCCCTGTTACACACCTTTTAATACGCTGCTTTCGCTACTGCTTCCACTTCCGGACGt                                                                                     >  2:683429/1‑150 (MQ=255)
                                                                taaaGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGttt                                                                                   <  1:1301827/149‑1 (MQ=255)
                                                                   atataataCCATTTAAATTCATAAAATTACATTCACATACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTATCACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAg                                                                                <  1:1160281/148‑1 (MQ=255)
                                                                     aTAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGa                                                                              >  1:274729/1‑150 (MQ=255)
                                                                        aatccATTTAAATTCATATAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACCGGACAGGAAATTTCTGCCCTGTAAGTCACCTTTTATTACGCTTATTTCGGTACTGCGTCCTGTTCCAGAGGTTTCAGGatgt                                                                           <  2:924724/147‑4 (MQ=255)
                                                                        aaaCCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAgg                                                                           >  1:909931/1‑150 (MQ=255)
                                                                              tttAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAgg                                                                     <  2:1157699/150‑1 (MQ=255)
                                                                               ttAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGc                                                                    <  2:1027080/150‑1 (MQ=255)
                                                                                tAAATTCATAAAATTTCATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGcc                                                                   >  1:1045442/1‑150 (MQ=255)
                                                                                 aaaTTCTTAAATTTACATTCAAAAAGCGGTGATTCTCTTTGCGCTCATCATATGTTTCAGGACAGGAAATTTCTGCCTTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCa                                                                  <  2:641609/150‑1 (MQ=255)
                                                                                  aaTTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCaa                                                                 <  1:94160/150‑1 (MQ=255)
                                                                                  aaTTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCaa                                                                 >  1:1125394/1‑150 (MQ=255)
                                                                                    ttCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAACTTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAAc                                                               <  1:86317/150‑1 (MQ=255)
                                                                                      cATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAAccc                                                             >  2:358373/1‑150 (MQ=255)
                                                                                         aaaaTTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGcc                                                          <  1:925953/150‑1 (MQ=255)
                                                                                                cATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCg                                                   >  1:797721/1‑150 (MQ=255)
                                                                                                   tCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTAc                                                >  2:583200/1‑150 (MQ=255)
                                                                                                     acaAACCGTTGATTCTCATTGCGCGCATCATATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTAGCTACTGCGTCAACTTGCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCAGCAGCGTAcct                                              <  2:878736/150‑2 (MQ=255)
                                                                                                     acaAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCg                                              >  2:339476/1‑150 (MQ=255)
                                                                                                      caAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCgg                                             <  2:907028/150‑1 (MQ=255)
                                                                                                          ccGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCaat                                         >  2:1040674/1‑150 (MQ=255)
                                                                                                            ggTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCaataa                                       <  1:1295800/150‑1 (MQ=255)
                                                                                                            ggTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGATGCTTTCGCTACTGCGTACACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCCGCTGTCCGGCaataa                                       >  2:811308/1‑150 (MQ=255)
                                                                                                             gTCATTCTCTTTGCGCTCATGAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCaataat                                      <  2:756062/150‑1 (MQ=255)
                                                                                                               cATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTg                                    <  2:699938/150‑1 (MQ=255)
                                                                                                                 ttCTCTTTGCGCTCATCAAATGTTACAGGACAGGAACTTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCt                                  <  1:133264/150‑1 (MQ=255)
                                                                                                                     ctTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATAGCTACCa                              >  2:1028055/1‑150 (MQ=255)
                                                                                                                              catcaAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCa                     <  1:607605/150‑1 (MQ=255)
                                                                                                                              catcaAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCa                     >  2:1279923/1‑150 (MQ=255)
                                                                                                                              catcaAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCa                     >  2:1268137/1‑150 (MQ=255)
                                                                                                                                  gtgtGTTACAGGGCAGAAATTTCCTGTCCTGTAACATACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATAc                 >  1:181464/4‑150 (MQ=39)
                                                                                                                                   aaTGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACTCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATAcc                <  1:674449/150‑1 (MQ=255)
                                                                                                                                   aaTGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACTCAATAcc                <  1:1127632/150‑1 (MQ=255)
                                                                                                                                   aaTGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATAcc                <  1:318691/150‑1 (MQ=255)
                                                                                                                                      gTTACAGGGCAGAAATTTCCTGTCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTTCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGc             >  2:639377/1‑150 (MQ=255)
                                                                                                                                      gTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCGCTTCCGGACGTTTCAGGAAGGCATAGGCCACACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTACCCCAATACCGGc             >  1:886805/1‑150 (MQ=255)
                                                                                                                                      gTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCTGACTTTTCAGGAAGGCATAGGCCAAACACGACACCAGCGTAACGGCAATTAATGCTACCAGGTAACCAAATACCGGc             >  1:887418/1‑150 (MQ=255)
                                                                                                                                       ttACATGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCg            <  1:447473/150‑1 (MQ=255)
                                                                                                                                       ttACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCg            <  1:250663/150‑1 (MQ=255)
                                                                                                                                           aggacaggaAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACAAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAAt        >  1:1300976/1‑150 (MQ=255)
                                                                                                                                            ggacaggaAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATa       >  1:477934/1‑150 (MQ=255)
                                                                                                                                            ggacaggaAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATa       >  2:262939/1‑150 (MQ=255)
                                                                                                                                             gacaggaTATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTATCCCAATACCGGCGTAATAg      <  1:302695/150‑1 (MQ=255)
                                                                                                                                             gacaggaAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAg      <  1:4821/150‑1 (MQ=255)
                                                                                                                                             gacaggaAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAg      <  2:909931/150‑1 (MQ=255)
                                                                                                                                                 ggaAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTACCCCAATACCGGCGTAATAGCGcc  >  2:963669/1‑150 (MQ=255)
                                                                                                                                                 ggaAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGcc  <  1:798108/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCTGCCCTGTAACACACCTTTTATTACGCTGCTTTCGCTACTGCGTCCACTTCCGGACGTTTCAGGAAGGCATAGGCCAAACCCGCCACCAGCGTACCGGCAATAATTGCTACCAGGTAACCCAATACCGGCGTAATAGCGCC  >  NC_000913/2259544‑2259838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: