Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,323,275 T→A 7.3% intergenic (+24/‑172) atoC → / → atoD fused response regulator of ato operon, in two‑component system with AtoS: response regulator/sigma54 interaction protein/acetyl‑CoA:acetoacetyl‑CoA transferase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,323,2750TA 7.3% 233.3 / 5.8 87intergenic (+24/‑172)atoC/atoDfused response regulator of ato operon, in two‑component system with AtoS: response regulator/sigma54 interaction protein/acetyl‑CoA:acetoacetyl‑CoA transferase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (43/37);  new base A (4/3);  total (47/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

ATTATGGAAGTGCTGGAACAACAAGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACG  >  NC_000913/2323129‑2323424
                                                                                                                                                  |                                                                                                                                                     
atTATGGAAGTGCTGGAACAACAAGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTg                                                                                                                                                    >  2:1008400/1‑150 (MQ=255)
   aTGGAAGTGCTGGAACAACAAGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGcac                                                                                                                                                 >  2:583081/1‑150 (MQ=255)
   aTGGAAGTGCTGGAACAACAAGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGcac                                                                                                                                                 <  1:386416/150‑1 (MQ=255)
        aGTGCTGGAACAACAAGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTgc                                                                                                                                            >  1:721270/1‑150 (MQ=255)
        aGTGCTGGAACAACAAGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTgc                                                                                                                                            >  2:673233/1‑150 (MQ=255)
         cggCTGGCACAACAAGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATCCGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTgcg                                                                                                                                           <  2:183568/148‑1 (MQ=255)
          tGCTGGAACAACAAGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTgcgc                                                                                                                                          <  1:1194017/150‑1 (MQ=255)
          tGCTGGAACAACAAGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTgcgc                                                                                                                                          <  2:265645/150‑1 (MQ=255)
             tGGAACAACAAGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAAt                                                                                                                                       >  2:387665/1‑150 (MQ=255)
              ggAACAACAAGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAAtt                                                                                                                                      >  1:443769/1‑150 (MQ=255)
              ggAACAACAAGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAAtt                                                                                                                                      >  1:443367/1‑150 (MQ=255)
              ggAACAACAAGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATACGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCCCAAtt                                                                                                                                      >  2:501181/1‑150 (MQ=255)
                    acaaGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGc                                                                                                                                <  1:1216513/150‑1 (MQ=255)
                    acaaGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAAATTGCGCACTGTGCAAATTTCTGc                                                                                                                                <  1:1286512/150‑1 (MQ=39)
                      tggagtttatacatcaatgcacggcgactgatgcccagcattaaagcagtgcATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACGTGCTATGCAGAAAATTGCGCACTGTGCAAATTTCTGCAt                                                                                                                              >  1:96018‑M2/53‑150 (MQ=255)
                        gaagGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAAATTGCGCACTGTGCAAATTTCTGCATAg                                                                                                                            <  1:820416/150‑1 (MQ=39)
                         aagGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGc                                                                                                                           <  1:1199491/150‑1 (MQ=255)
                         aagGAAACCGAACCCGCACTGCATTAATGCTGGGAATCAGTCGCCGTTCATTGATGTATAAACTCCAGGAATACGTTATCGATACGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGc                                                                                                                           <  2:46553/150‑1 (MQ=255)
                         aagGAAACCGAACCCGCACAGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGc                                                                                                                           <  2:1105945/150‑1 (MQ=255)
                          agGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGcc                                                                                                                          >  2:516786/1‑150 (MQ=255)
                             aaaCCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGATATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGca                                                                                                                       >  1:482455/1‑149 (MQ=255)
                              aaCCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCAGTGCATAGATGTATAAACTCCAGGAATACTGTATCGAGCCGGCGGATGTATAACACAAAAACTTTCAATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCGGCTc                                                                                                                      >  2:253418/1‑150 (MQ=255)
                                 catcaatgcacggcgactgatgcccagcattaaagcagtgcATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCAtt                                                                                                                   >  2:1063742‑M2/42‑150 (MQ=255)
                                   tcaatgcacggcgactgatgcccagcattaaagcagtgcATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATtct                                                                                                                 >  1:597264‑M2/40‑150 (MQ=255)
                                     cccGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTcc                                                                                                               <  1:512705/150‑1 (MQ=255)
                                     cccGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTcc                                                                                                               <  2:969503/150‑1 (MQ=255)
                                       cGCACTGCTTTAATTCTGGGCATCAGTCGCCGTTCATTGATGTATAAAATCCAGGAATACGGTATAGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCtt                                                                                                             <  2:626036/150‑1 (MQ=255)
                                       cGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCtt                                                                                                             >  1:773602/1‑150 (MQ=255)
                                          ACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTAGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTtata                                                                                                          >  1:1298728‑M1/1‑150 (MQ=255)
                                          ACTGCTTTAATGCGGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTtata                                                                                                          >  1:1125634‑M1/1‑150 (MQ=255)
                                                      cTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATACCCATACa                                                                                              >  1:148948/1‑150 (MQ=255)
                                                        gggCATGATGCGCCGCGCCTTGATGTATAAACTCCAGGAATACTGTCTCGATCCGGCGGATGTATAACACCAAACCTTGCTATGCAGATATTTGCTGCGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCGATCCAAt                                                                                            <  2:135337/150‑1 (MQ=255)
                                                             tCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATc                                                                                       <  1:392894/150‑1 (MQ=255)
                                                              cAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATcc                                                                                      >  2:332660/1‑150 (MQ=255)
                                                                   gCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCa                                                                                 <  1:968698/150‑1 (MQ=255)
                                                                   gCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCa                                                                                 <  1:979569/150‑1 (MQ=255)
                                                                   gCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACAACAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAAACCCCATCCAATTTATCCCTTCa                                                                                 >  2:1172016/1‑150 (MQ=255)
                                                                     cGTGCATTGATGTATAAACTCCAGGAATACGGTATCGCTCCGGCGGCTGGATCACACCAAAACTTGCTATGCAGACATTTGCACAGTGCGCCATTTTCTGCATAGCCGCTCATTCGCCTTATAAATCGCCTTACAATGTCTCCCTTCata                                                                               >  1:206444/1‑150 (MQ=255)
                                                                     cGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATGTTCTGCATAGCCGCTCATTCTCCTTATAAATACCCATCCAATTTCTCCCTTCata                                                                               >  1:69923/1‑150 (MQ=255)
                                                                      gtgcATTGATGTATAAACTCCAGGAAGACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCACAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCatat                                                                              >  1:966090‑M2/5‑150 (MQ=255)
                                                                       tgcATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAACTCCCCATCCAATTTATCCCTTCATAtt                                                                             >  2:1158370‑M2/4‑150 (MQ=255)
                                                                        gcATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTc                                                                            >  1:914709‑M2/3‑150 (MQ=255)
                                                                         cagtGATGTATAACCTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGAAGAGCCGCGCATTCTCCTTTAAAATCCCGATACATTTTATCCCTTCatatgaa                                                                           >  2:75760/4‑147 (MQ=255)
                                                                           ttGATGTATAAACTCCAGGAATACTGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGAGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAt                                                                         <  1:173042/150‑1 (MQ=255)
                                                                           ttGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATTCAGAAATTTGCACAGTGCGCAATATTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAt                                                                         <  1:1171454/150‑1 (MQ=255)
                                                                           ttGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAt                                                                         <  1:1176761/150‑1 (MQ=255)
                                                                            tGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAtt                                                                        <  2:400792/150‑1 (MQ=255)
                                                                            tGATGTATAAACTACAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAtt                                                                        <  2:1038622/150‑1 (MQ=255)
                                                                             gATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTa                                                                       >  1:470547/1‑150 (MQ=255)
                                                                                   taAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATCCAATTAGTTaaa                                                                 <  2:773602/150‑1 (MQ=255)
                                                                                     aaCTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATa                                                               >  2:888394/1‑150 (MQ=255)
                                                                                     aaCTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAACCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATa                                                               >  1:730046/1‑150 (MQ=255)
                                                                                       cTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAAc                                                             >  2:943052/1‑150 (MQ=255)
                                                                                         ccAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTa                                                           <  1:270260/150‑1 (MQ=255)
                                                                                            ggAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTTTGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAAt                                                        <  1:875070/150‑1 (MQ=255)
                                                                                              aaaactgTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCATCATATTCAATTAGTTAAATAACTAAATcc                                                      <  1:934171/144‑1 (MQ=255)
                                                                                               aTACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCa                                                     >  1:1301853/1‑150 (MQ=255)
                                                                                                 aCGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGAGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCaat                                                   <  2:951641/150‑1 (MQ=255)
                                                                                                  cGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCAATCCAATTTATCACTTCATATTCAATTAGTTAAATAACTAAATCCaata                                                  >  1:613677/1‑150 (MQ=255)
                                                                                                           tCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCAtta                                         >  2:971342/1‑149 (MQ=255)
                                                                                                            ccGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTTCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCt                                        >  2:884279/1‑150 (MQ=255)
                                                                                                            ccGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCt                                        <  2:148661/150‑1 (MQ=255)
                                                                                                            ccGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTACTGCATATCCGCTCATTCTACTAAAAAATCACCAACCAATTTATCCATACATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTca                                        >  1:797680/1‑149 (MQ=255)
                                                                                                             cggcggATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCAAAGCCGCTCATTATCATTATAAAACCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATACAATAATCTCATTATg                                       >  2:984295/1‑150 (MQ=255)
                                                                                                                 ggATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCAc                                   >  1:665170/1‑150 (MQ=255)
                                                                                                                  gATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACt                                  >  2:1019653/1‑150 (MQ=255)
                                                                                                                        taACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCtt                            <  1:1280256/150‑1 (MQ=255)
                                                                                                                              aaaaaCTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTAtt                      <  1:790685/149‑1 (MQ=255)
                                                                                                                               aaaaCTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTg                     >  1:1089049/1‑150 (MQ=255)
                                                                                                                               aaaaCTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTg                     <  1:406496/150‑1 (MQ=255)
                                                                                                                                  aCTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCAAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCt                  >  1:470194/1‑150 (MQ=255)
                                                                                                                                   cTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTACTTAAATAACTAAATCCAAAAATCTCATTCAGGCACTCCCCATGCTATTGCCTg                 >  2:1073192/1‑150 (MQ=255)
                                                                                                                                    ttGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGa                >  2:1054039/1‑150 (MQ=255)
                                                                                                                                     tGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGAc               <  1:302847/150‑1 (MQ=255)
                                                                                                                                       cTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTg             <  1:423988/150‑1 (MQ=255)
                                                                                                                                       cTATGCAGAAAATTGCACAGTGCGCTATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTATATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTg             <  2:705050/150‑1 (MQ=255)
                                                                                                                                        tATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGt            <  1:669808/150‑1 (MQ=255)
                                                                                                                                            cAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCATTGCTATTGCCTGACTGTAccc        >  2:477858/1‑150 (MQ=255)
                                                                                                                                            cAGAAAATTGCGCACTGTGCAAATTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCCCTCCCCTTGCTATTGCATGACTGTAcca        >  1:251162/1‑149 (MQ=255)
                                                                                                                                            cAGAAAATTGCGCACTGTGCAAATTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTAccc        >  2:545168/1‑150 (MQ=255)
                                                                                                                                             aGAAATTTGCACAGTGCGCAATTTTCTTCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCca       >  2:506270/1‑150 (MQ=255)
                                                                                                                                             aGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACAAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCca       <  2:550897/150‑1 (MQ=255)
                                                                                                                                             aGAAAATTGCGCACTGTGCAAATTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCca       >  1:385237/1‑150 (MQ=255)
                                                                                                                                              gAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCcac      <  2:584758/150‑1 (MQ=255)
                                                                                                                                               aaaTTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTTCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCcaca     <  2:267705/150‑1 (MQ=255)
                                                                                                                                                 aTTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATTCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAAc   <  2:938787/150‑1 (MQ=255)
                                                                                                                                                 aTTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAAc   <  2:614017/150‑1 (MQ=255)
                                                                                                                                                  tttGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACg  >  1:918203/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
ATTATGGAAGTGCTGGAACAACAAGAAGGAAACCGAACCCGCACTGCTTTAATGCTGGGCATCAGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACG  >  NC_000913/2323129‑2323424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: