Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,406,378 G→C 7.2% A88A (GCC→GCG) ‡ lrhA ← transcriptional repressor of flagellar, motility and chemotaxis genes

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,406,3780GC 7.2% 275.1 / 11.9 97A88A (GCC→GCG) ‡lrhAtranscriptional repressor of flagellar, motility and chemotaxis genes
Reads supporting (aligned to +/- strand):  ref base G (36/54);  new base C (3/4);  total (39/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.30e-01

CATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGAC  >  NC_000913/2406230‑2406525
                                                                                                                                                    |                                                                                                                                                   
cATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAgg                                                                                                                                                    >  2:295251/1‑150 (MQ=255)
   tACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCt                                                                                                                                                 >  2:359716/1‑150 (MQ=255)
      gTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCtcat                                                                                                                                              >  2:1158412/1‑150 (MQ=255)
       tttAACTCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCAAGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCtcatc                                                                                                                                             <  2:448447/150‑1 (MQ=255)
        ttAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCtcatca                                                                                                                                            >  1:430165/1‑150 (MQ=255)
        ttAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCtcatca                                                                                                                                            <  2:764912/150‑1 (MQ=255)
         tAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGTAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGATCATTAATGATGAGCAGGCCTcatcat                                                                                                                                           <  2:278690/150‑1 (MQ=255)
           aCGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTa                                                                                                                                         <  2:1111565/150‑1 (MQ=255)
           aCGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTa                                                                                                                                         >  1:930207/1‑150 (MQ=255)
           aCGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTCAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAAAGATGAGCAGGCCTCATCATTa                                                                                                                                         >  1:311250/1‑150 (MQ=255)
            cgcgGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTATCAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTaa                                                                                                                                        >  1:340211/1‑150 (MQ=255)
            cgcgGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTATGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTaa                                                                                                                                        >  1:450088/1‑150 (MQ=255)
            cgcgGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGAAAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTaa                                                                                                                                        >  2:1270522/1‑150 (MQ=255)
             gcgTACAACCAGCGATAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAATATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGGCCTGCTCATCATTaat                                                                                                                                       <  2:860623/150‑2 (MQ=255)
             gcgGACATCCAGCGCTAATTTCGTATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGGCCTGCTCATCATTaat                                                                                                                                       <  2:249065/150‑2 (MQ=255)
              cgGACATCCAGCGCTAATTTCGTATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGGCCTGCTCATCATTaatg                                                                                                                                      <  2:554892/150‑3 (MQ=255)
              cgGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTTATGATGAGCAGGCCTCATCATTaaaa                                                                                                                                      >  2:674790/1‑150 (MQ=255)
              cgGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTaaaa                                                                                                                                      >  2:1090233/1‑150 (MQ=255)
               ggACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGGCCTGCTCATCATTaatg                                                                                                                                      <  1:569774/149‑3 (MQ=255)
               ggACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGGCCTGCTCATCATTaatg                                                                                                                                      >  2:569774/1‑147 (MQ=255)
               ggACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAAc                                                                                                                                     <  2:542956/150‑1 (MQ=255)
                gACATCCAGCGCTAATTTCGGATAAACCGAACTAACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACg                                                                                                                                    <  2:633007/150‑1 (MQ=255)
                    tCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAATGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAgg                                                                                                                                <  1:22502/150‑1 (MQ=255)
                    accACCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAgg                                                                                                                                <  1:68568/149‑1 (MQ=255)
                     ccAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGa                                                                                                                               >  1:359645/1‑150 (MQ=255)
                        gcgcTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGAttt                                                                                                                            >  1:1173390/1‑150 (MQ=255)
                           cTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTcc                                                                                                                         <  2:74960/150‑1 (MQ=255)
                           cTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTcc                                                                                                                         <  2:164653/150‑1 (MQ=255)
                           cTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCt                                                                                                                        <  1:799322/150‑1 (MQ=255)
                             aaTTTCGGATAACCCGAAATCACGCTATTTAACAGGAAAGTTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATAAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTg                                                                                                                       <  2:935485/150‑1 (MQ=255)
                              aTTTCGGATAAACCGAACTCACGCTATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATAAGACCTGATGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTgg                                                                                                                      <  2:341608/150‑1 (MQ=255)
                              aTTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTgg                                                                                                                      <  2:307814/150‑1 (MQ=255)
                                   ggATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTaa                                                                                                                 >  2:517374/1‑150 (MQ=255)
                                    gATAAACCGAACTCACGCGAGTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCGCTTAAGATTAGTGAACATTAATGATGCGCAGGCCTCATCTTTAAAACGCCGGATTTTCCTGGCGTAAc                                                                                                                >  2:64769/1‑150 (MQ=255)
                                     aTAAACCGAACTCACGCGATTTATCAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTCGCGTAAcc                                                                                                               >  1:166270/1‑150 (MQ=255)
                                      aaaaCCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCa                                                                                                              <  1:926156/149‑1 (MQ=255)
                                          ccaaaCTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCaagaa                                                                                                          <  2:1158850/147‑1 (MQ=255)
                                            gAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGt                                                                                                        <  2:15359/150‑1 (MQ=255)
                                             aaCTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGtt                                                                                                       <  1:594152/150‑1 (MQ=255)
                                              aCTCACGCGATTTAACAGGAAAGCTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCGGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTg                                                                                                      <  2:1002711/150‑1 (MQ=255)
                                               cTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGa                                                                                                     <  1:46613/150‑1 (MQ=255)
                                                tCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCTCCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGaa                                                                                                    <  1:257216/150‑1 (MQ=255)
                                                 cACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAAt                                                                                                   >  1:242724/1‑150 (MQ=255)
                                                  aCGCTATTTAAAAGGAAAGGTAAGATCGAATCGGCAGATTCATCTGAAGCACCTATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATGTTCCTGGCGTAACCAAGAAGTTGAATg                                                                                                  <  2:1199431/150‑1 (MQ=255)
                                                  aCGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATg                                                                                                  <  1:498107/150‑1 (MQ=255)
                                                   cgcgATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGc                                                                                                 >  2:389815/1‑150 (MQ=255)
                                                     cgATTTAACAGGAAAGGTAAGATCGTATCGGCATATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCa                                                                                               <  1:1017497/150‑1 (MQ=255)
                                                          tAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTc                                                                                          >  2:1317696/1‑150 (MQ=255)
                                                             cAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGt                                                                                       <  1:720404/150‑1 (MQ=255)
                                                                 aaaGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAAc                                                                                   <  1:524954/150‑1 (MQ=255)
                                                                 aaaGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAAc                                                                                   <  1:50558/150‑1 (MQ=255)
                                                                     gTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAAAGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGtt                                                                               <  2:66119/150‑1 (MQ=255)
                                                                         gATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTttgtt                                                                           >  2:1103920/1‑150 (MQ=255)
                                                                          ttCGTATCGGCAGATTCATCTGAATCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTttgttg                                                                          <  2:595654/149‑1 (MQ=255)
                                                                          aTCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTACTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTttgttg                                                                          >  1:1219145/1‑150 (MQ=255)
                                                                           tCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGc                                                                         <  2:430165/150‑1 (MQ=255)
                                                                           tCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGc                                                                         <  1:477284/150‑1 (MQ=255)
                                                                               aTCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCTTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGccc                                                                     >  2:471948/1‑150 (MQ=255)
                                                                               aTCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGAcc                                                                     <  1:1041980/150‑1 (MQ=255)
                                                                                tCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCg                                                                    <  2:928615/150‑1 (MQ=255)
                                                                                 cGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGt                                                                   <  1:359716/150‑1 (MQ=255)
                                                                                      gATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTAGCGACCGTGACGa                                                              >  2:1200371/1‑150 (MQ=255)
                                                                                           aTCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGaa                                                         >  2:949069/1‑150 (MQ=255)
                                                                                            tCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAAc                                                        <  2:923443/150‑1 (MQ=255)
                                                                                               gAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGt                                                     <  1:183845/150‑1 (MQ=255)
                                                                                               gAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGt                                                     <  2:1234192/150‑1 (MQ=255)
                                                                                                aaGCACCGATAGTTAAAACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGtt                                                    <  1:282124/150‑1 (MQ=255)
                                                                                                     ccGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTc                                               <  2:750472/150‑1 (MQ=255)
                                                                                                     ccGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTc                                               >  1:407762/1‑150 (MQ=255)
                                                                                                     ccGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTATc                                               >  1:401914/1‑150 (MQ=255)
                                                                                                      cGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTcc                                              <  2:523701/150‑1 (MQ=255)
                                                                                                       gATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTccc                                             <  1:395676/150‑1 (MQ=255)
                                                                                                       gATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTccc                                             <  2:930207/150‑1 (MQ=255)
                                                                                                            ttAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGa                                        >  2:328403/1‑150 (MQ=255)
                                                                                                                    cccTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGCACAGTTCTTTCCCAACGAGTTGTTcc                                >  2:144383/1‑150 (MQ=255)
                                                                                                                    cccTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTcc                                <  1:994369/150‑1 (MQ=255)
                                                                                                                    cccTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTcc                                >  2:1132182/1‑150 (MQ=255)
                                                                                                                     ccTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCa                               >  2:1087398/1‑150 (MQ=255)
                                                                                                                      cTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAg                              >  2:1058690/1‑150 (MQ=255)
                                                                                                                              tACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCa                      <  1:1090233/150‑1 (MQ=255)
                                                                                                                               aCTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCAt                     <  2:1199689/150‑1 (MQ=255)
                                                                                                                                  gcaCATTAATGAAGCGCAGGCCTGAGCATTAAAACTCAGGATTTTCCTGGCGTAACAAATAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTg                  <  1:788029/148‑1 (MQ=255)
                                                                                                                                  gAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGATTGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTg                  >  1:1255252/1‑150 (MQ=255)
                                                                                                                                  gAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTg                  >  2:904098/1‑150 (MQ=255)
                                                                                                                                   aaCATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGc                 <  1:911239/150‑1 (MQ=255)
                                                                                                                                      aTTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGa              <  1:174540/150‑1 (MQ=255)
                                                                                                                                       ttAATGATGAGGCCTGCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGAc             >  2:210316/1‑150 (MQ=255)
                                                                                                                                       ttAATGATGAGGCCTGCTCATAATTAAAACGCAGGATTTACAGGGCGTAACCAAGATGATGAATGCAATGTTCACTTAACAGTTTGTTGCGACCGTGAAGAGCGAACAGTACTTTCCCAACGAGTTGTTCCAGACGCTGCATTtgatgaa             >  2:210320/1‑149 (MQ=255)
                                                                                                                                        tAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACt            <  2:242724/150‑1 (MQ=255)
                                                                                                                                        tAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACt            <  1:929279/150‑1 (MQ=255)
                                                                                                                                        tAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACt            <  2:299752/150‑1 (MQ=255)
                                                                                                                                         aaTGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGCCGCTGCATTTGCTGACtt           >  1:1129739/1‑150 (MQ=255)
                                                                                                                                         aaTGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACtt           >  2:582438/1‑150 (MQ=255)
                                                                                                                                         aaTGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTATTTCCCAACGAGGTGTTCCAGACGCTGCATTTGCTGCCtt           >  2:835757/1‑150 (MQ=255)
                                                                                                                                          atgatgAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTa          <  1:293894/150‑1 (MQ=255)
                                                                                                                                                aGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTAcggcgg    <  2:579187/150‑1 (MQ=255)
                                                                                                                                                aGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTAcggcgg    >  2:348318/1‑150 (MQ=255)
                                                                                                                                                  cAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGAc  <  2:990791/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CATTACGTTTAACGCGGACATCCAGCGCTAATTTCGGATAAACCGAACTCACGCGATTTAACAGGAAAGGTAAGATCGTATCGGCAGATTCATCTGAAGCACCGATAGTTAATACGCCCTGAAGATTACTGAACATTAATGATGAGCAGGCCTCATCATTAAAACGCAGGATTTTCCTGGCGTAACCAAGAAGTTGAATGCCATGTTCAGTTAACAGTTTGTTGCGACCGTGACGAGCGAACAGTTCTTTCCCAACGAGTTGTTCCAGACGCTGCATTTGCTGACTTACGGCGGAC  >  NC_000913/2406230‑2406525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: