Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,416,945 C→T 6.3% intergenic (+54/‑136) pta → / → yfcC phosphate acetyltransferase/putative inner membrane transporter; C4‑dicarboxylate anaerobic carrier family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,416,9450CT 6.3% 294.5 / 7.0 95intergenic (+54/‑136)pta/yfcCphosphate acetyltransferase/putative inner membrane transporter; C4‑dicarboxylate anaerobic carrier family protein
Reads supporting (aligned to +/- strand):  ref base C (36/53);  new base T (2/4);  total (38/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

AGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACCACTTTGTTTTGTGCAAGGGAATATTTGCGCTATGTCCGCAATC  >  NC_000913/2416800‑2417092
                                                                                                                                                 |                                                                                                                                                   
aGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTAtt                                                                                                                                                 >  1:492457/1‑150 (MQ=255)
    ggTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACCCCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTcag                                                                                                                                             >  1:668842/1‑148 (MQ=255)
    ggTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCg                                                                                                                                             <  1:891045/150‑1 (MQ=255)
     gTTCACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCgg                                                                                                                                            <  2:1158832/150‑1 (MQ=255)
     gTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTAGACCAGCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGGAGCTTTGCGCTGCGGATATCTGAACCGGAAATAGTGATTATTTCCgg                                                                                                                                            <  2:1246316/150‑1 (MQ=255)
     gTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAGTGATTATTTCCgg                                                                                                                                            <  2:339594/150‑1 (MQ=255)
      ttAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAGTGATTATTTCCGGt                                                                                                                                           <  2:454576/150‑1 (MQ=255)
      ttAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAGTGATTATTTCCGGt                                                                                                                                           <  1:689795/150‑1 (MQ=255)
      ttAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGt                                                                                                                                           <  1:1202000/150‑1 (MQ=255)
      ttAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGt                                                                                                                                           <  1:287833/150‑1 (MQ=255)
      ttAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCAATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGt                                                                                                                                           <  2:671338/150‑1 (MQ=255)
       tAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGtt                                                                                                                                          <  2:1052028/150‑1 (MQ=255)
            aCCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTa                                                                                                                                     >  1:431653/1‑150 (MQ=255)
            aCCTCTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTGGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAATTTATCACTTTTTCCGGgtgtttt                                                                                                                                     >  2:62405/1‑149 (MQ=255)
             ccttgcccGTGGCGCACTGGTTGACGATATCGTCTACACCAGCGCGCTGACTGAGATTCAGTCTGCTCAGCTTCAGTATTCTCGTCATCATCCGCAGCTTTGCGATGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTAt                                                                                                                                    <  2:551145/145‑1 (MQ=255)
             ccTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTAt                                                                                                                                    <  2:85224/150‑1 (MQ=255)
               tGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATtc                                                                                                                                  <  1:723073/150‑1 (MQ=255)
                gTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATtct                                                                                                                                 >  1:1030314/1‑150 (MQ=255)
                gTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATtct                                                                                                                                 >  1:745509/1‑150 (MQ=255)
                  cccGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTctct                                                                                                                               <  2:733623/150‑1 (MQ=255)
                        gcgcACTGGTTGACGATATCGTCAACACCATCGCGCTGAATGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCCGAAATAATCACTATTTCCGGTTTAATATTCTCTTAAttt                                                                                                                         >  1:704661/1‑150 (MQ=255)
                          gcACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGc                                                                                                                       <  2:293964/150‑1 (MQ=255)
                           cACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCa                                                                                                                      <  2:76420/150‑1 (MQ=255)
                            aCTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCAt                                                                                                                     <  2:664559/150‑1 (MQ=255)
                            aCTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCAt                                                                                                                     <  2:841357/150‑1 (MQ=255)
                            aCTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCAt                                                                                                                     <  1:201147/150‑1 (MQ=255)
                            aCTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCAt                                                                                                                     <  1:1197761/150‑1 (MQ=255)
                             cTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCAtt                                                                                                                    <  2:1157471/150‑1 (MQ=255)
                             cTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCAtt                                                                                                                    >  1:612280/1‑150 (MQ=255)
                             cTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCAtt                                                                                                                    <  2:556649/150‑1 (MQ=255)
                                 ttGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATc                                                                                                                <  2:143561/150‑1 (MQ=255)
                                  tGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATcc                                                                                                               <  2:1266355/150‑1 (MQ=255)
                                      gATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTc                                                                                                           <  2:511746/150‑1 (MQ=255)
                                       atatCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCt                                                                                                          <  2:401577/150‑1 (MQ=255)
                                           cGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACAATTTACGGTTTTATATTCTCTTAATTTGCATTAATCCTTTCTGAtt                                                                                                      >  2:1267227/1‑150 (MQ=255)
                                            gTCTAAACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATTATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTa                                                                                                     <  1:783647/150‑1 (MQ=255)
                                               tACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTAGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCt                                                                                                  >  1:467263/1‑150 (MQ=255)
                                                acacCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCtt                                                                                                 >  1:909202/1‑150 (MQ=255)
                                                acacCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTATGCGCTGCGTATATCTGAACCGGAAATAATCACTATTTCCGGATTTTTATTCTCTTAATATGAATTAATCCTTTCTGATTATCtt                                                                                                 >  2:1084958/1‑150 (MQ=255)
                                                  acCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGc                                                                                               >  2:492342/1‑150 (MQ=255)
                                                       cgcgCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAAc                                                                                          >  1:635656/1‑150 (MQ=255)
                                                       cgcgCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAAc                                                                                          <  2:392604/150‑1 (MQ=255)
                                                           cTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGAATTAATCCTTTCTGATTATCTTGCTTAACTgcg                                                                                      >  1:462396/1‑150 (MQ=255)
                                                            tGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTgcgc                                                                                     <  2:1008844/150‑1 (MQ=255)
                                                               cTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGc                                                                                  >  2:75100/1‑150 (MQ=255)
                                                                 gCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCAt                                                                                <  1:1142217/150‑1 (MQ=255)
                                                                  cGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATc                                                                               <  1:1189970/150‑1 (MQ=255)
                                                                   gATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCa                                                                              >  1:976366/1‑150 (MQ=255)
                                                                    aTTCAGTCTGCACAGCAGCAGTAATCTCGTGATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCaa                                                                             >  1:517112/1‑150 (MQ=255)
                                                                    aTTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCaa                                                                             <  2:1033701/150‑1 (MQ=255)
                                                                      tCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATg                                                                           <  2:76623/150‑1 (MQ=255)
                                                                        aGTCTGCAAAGAATCATTAATCTCTTAATCATCCGCAGCTTTGCGATGCTGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGaa                                                                         <  1:131572/150‑1 (MQ=255)
                                                                           cTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTg                                                                      <  1:1165366/150‑1 (MQ=255)
                                                                            tGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTgc                                                                     <  1:952670/150‑1 (MQ=255)
                                                                            tGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACGATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTgc                                                                     >  2:56590/1‑150 (MQ=255)
                                                                               acaGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGcc                                                                  >  2:389892/1‑150 (MQ=255)
                                                                                 agcagcagTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCAt                                                                >  1:287026/1‑150 (MQ=255)
                                                                                   cagcagTAATCTCGTCATCATACGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATcc                                                              <  1:195341/150‑1 (MQ=255)
                                                                                    agcagTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTGTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATccc                                                             >  1:1133239/1‑150 (MQ=255)
                                                                                          aaTCTCGTCATCATCCGCAGCTTTGCGCTTCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTg                                                       <  2:811481/150‑1 (MQ=255)
                                                                                          aaTCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTg                                                       <  2:1030314/150‑1 (MQ=255)
                                                                                          aaTCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTg                                                       >  2:351219/1‑150 (MQ=255)
                                                                                          aaTCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCACACtttt                                                       >  1:942434/1‑149 (MQ=255)
                                                                                             ctcGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCAt                                                    <  2:235456/150‑1 (MQ=255)
                                                                                               cGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATAc                                                  >  1:1030793/1‑150 (MQ=255)
                                                                                                    tcatcCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTAcc                                             <  2:1193318/150‑1 (MQ=255)
                                                                                                    tcatcCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTAcc                                             <  1:312903/150‑1 (MQ=255)
                                                                                                    tcatcCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTAcc                                             <  2:635656/150‑1 (MQ=255)
                                                                                                     catcCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACCa                                            >  2:996152/1‑150 (MQ=255)
                                                                                                        ccGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACCACtt                                         >  1:415383/1‑150 (MQ=255)
                                                                                                         cGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACCACttt                                        >  1:716446/1‑150 (MQ=255)
                                                                                                           cAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACCACtttgt                                      <  1:1301488/150‑1 (MQ=255)
                                                                                                             gCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACCACtttgttt                                    >  2:1192049/1‑150 (MQ=255)
                                                                                                              cTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACCACtttgtttt                                   <  1:1115471/150‑1 (MQ=255)
                                                                                                                 tGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACCACTTTGTTTtgtg                                <  2:492457/150‑1 (MQ=255)
                                                                                                                  gcgcTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACCACTTTGTTTTGTGc                               <  1:1125366/150‑1 (MQ=255)
                                                                                                                  gcgcTGCGGATATCTGAACCGGAAATAATCACTATATGCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACCACTTTGTTTTGTGc                               <  1:950112/150‑1 (MQ=255)
                                                                                                                   cgcTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCTTCAATGAATTGCGCCATCCCACTTTGCATACTTACCACTTTGTTTTGTGCa                              >  2:1211584/1‑150 (MQ=255)
                                                                                                                     cTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACCACTTTGTTTTGTGCAAg                            <  2:93410/150‑1 (MQ=255)
                                                                                                                      tGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACCACTTTGTTTTGTGCAAgg                           >  2:334182/1‑150 (MQ=255)
                                                                                                                       gCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACCACTTTGTTTTGTGCAAggg                          <  2:893375/150‑1 (MQ=255)
                                                                                                                       gCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACCACTTTGTTTTGTGCAAggg                          <  1:14433/150‑1 (MQ=255)
                                                                                                                        gggATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATATGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACCACTTTGTTTTGTGCAAGGGa                         <  2:741929/149‑1 (MQ=255)
                                                                                                                              tCTGAACCGGAAATAATCACTATTTGCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGATTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACAACTTTGTTTTGTGCAAGGGAATAttt                   >  1:801083/1‑150 (MQ=255)
                                                                                                                              tCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACCACTTTGTTTTGTGCAAGGGAATAttt                   <  2:909202/150‑1 (MQ=255)
                                                                                                                               cTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACCACTTTGTTTTGTGCAAGGGAATATTTg                  <  2:1145522/150‑1 (MQ=255)
                                                                                                                               aaaaaCCGGAAATAGTGATTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCAAATTTGCATACTTACCACTTTGTTTTGTGAAAGGGAATATTTg                  >  1:851460/4‑150 (MQ=255)
                                                                                                                                tGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACCACTTTGTTTTGTGCAAGGGAATATTTgc                 <  2:745509/150‑1 (MQ=255)
                                                                                                                                    ccGGAAATAGTGATTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCATTAATGATTATCTAGCTAAACTGCGCTGAATCAATGAATTGCTCCATCCCACTTTGCATACTTACCACATTGTATTGTGCAAGGGAATATTTGCGCTa             >  1:290043/1‑150 (MQ=255)
                                                                                                                                    ccGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACCACTTTGTTTTGTGCAAGGGAATATTTGCGCTa             <  1:434405/150‑1 (MQ=255)
                                                                                                                                       gAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACCACTTTGTTTTGTGCAAGGGAATATTTGCGCTATGt          >  1:802118/1‑150 (MQ=255)
                                                                                                                                        aaaTAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACCACTTTGTTTTGTGCAAGGGAATATTTGCGCTATGTc         <  1:812180/150‑1 (MQ=255)
                                                                                                                                         aataatCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACCACTTTGTTTTGTGCAAGGGAATATTTGCGCTATGTcc        >  2:1065051/1‑150 (MQ=255)
                                                                                                                                            aatCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACCACTTTGTTTTGTGCAAGGGAATATTTGCGCTATGTCCGCa     >  2:470527/1‑150 (MQ=255)
                                                                                                                                               cACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGATGCTTCATTGATTTGCGACATACCACTTTGCATTCTTACCAATTTGTTTTGTGCAAGGGAATATTTGCGCTATGTCCGCAATc  >  1:33163/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
AGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCTCGTCATCATCCGCAGCTTTGCGCTGCGGATATCTGAACCGGAAATAATCACTATTTCCGGTTTTTTATTCTCTTAATTTGCATTAATCCTTTCTGATTATCTTGCTTAACTGCGCTGCATCAATGAATTGCGCCATCCCACTTTGCATACTTACCACTTTGTTTTGTGCAAGGGAATATTTGCGCTATGTCCGCAATC  >  NC_000913/2416800‑2417092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: