Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,430,871 C→T 7.9% intergenic (‑108/+151) cvpA ← / ← dedD colicin V production protein/membrane‑anchored periplasmic protein involved in septation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,430,8710CT 7.9% 208.4 / 5.9 76intergenic (‑108/+151)cvpA/dedDcolicin V production protein/membrane‑anchored periplasmic protein involved in septation
Reads supporting (aligned to +/- strand):  ref base C (42/27);  new base T (2/4);  total (45/31)
Fisher's exact test for biased strand distribution p-value = 2.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.92e-01

AAGCAATCACCGCGATTATGGCGTAATCAATCCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACC  >  NC_000913/2430724‑2431018
                                                                                                                                                   |                                                                                                                                                   
aaGCAATCACCGCGATTATGGCGTAATCAATCCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCATAGGGATTTCCTTCCCGCGCATCAATAAAAATGgcgcag                                                                                                                                                   >  1:58950/1‑148 (MQ=255)
   cAATCACCGCGATTATGGCGTAATCAATCCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGaaa                                                                                                                                                >  2:810109/1‑150 (MQ=255)
      acACCGCGATTATCGCGTAATCAATCCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGaaaaaa                                                                                                                                             <  2:1184004/149‑1 (MQ=255)
       cACCGCGATTATGGCGTAATCAATCCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCTCTGAAAAAat                                                                                                                                            >  1:373545/1‑150 (MQ=255)
       cACCGCGATTATGGCGTAATCAATCCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAat                                                                                                                                            <  2:270216/150‑1 (MQ=255)
        aCCGCGCTTATTGCGTTATCAATCCAGACAATATGTGTCCCAAGATTTTACTCCCTGTCATCGTGTTCGGGTCGCTTTCTAACAGAATAAGAAAACGTTTGCGTAGGGATTGCCTTCCCGGGCATGAATAAAAATGGAGCTGaataaata                                                                                                                                           <  1:400487/150‑1 (MQ=255)
         ccGCGATTATGGCGTAATCAATCCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAatat                                                                                                                                          >  2:1061984/1‑150 (MQ=255)
          cgcgATTATGGCGTAATCAATCCAGACCATATGTGTCCCACGATTTTACGACATGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATAtt                                                                                                                                         >  2:584985/1‑150 (MQ=255)
            cgATTATGGCGTAATCAATCCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCa                                                                                                                                       <  1:1086728/150‑1 (MQ=255)
                  tGGCGTAATCAATCCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCa                                                                                                                                 >  1:67131/1‑150 (MQ=255)
                  tGGCGTAATCAATCCAGACCATATGTGTCCCACGATTTTACGCACTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCa                                                                                                                                 >  2:928804/1‑150 (MQ=255)
                   ggCGTAATCAATCCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGTTGAATATTTTTTCAGCGCCAt                                                                                                                                <  1:130915/150‑1 (MQ=255)
                     cGTAATCAATCCAGCCCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGTGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCAtag                                                                                                                              >  2:1143048/1‑148 (MQ=255)
                       tAATCAATCCAGACCATATGTGTCCCACGATTTTACGCCATGTCATCCTGTTCGGGGCGCATTCTAACAGAAAATGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGTAAAAATATTCAACGCGATCGAc                                                                                                                            >  1:655599/1‑150 (MQ=255)
                         atcaatcCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACtt                                                                                                                          >  2:1266337/1‑150 (MQ=255)
                          tcaatcCAGACCTTATGTGTACCACGATTTTACGCCCTGTCATCCTGTTCGGGTCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCTCTGAAAAAATATTCAACGCCATCGACttt                                                                                                                         <  1:883684/150‑1 (MQ=255)
                          tcaatcCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGCTTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATTTTCAACGCCGTCGACgtt                                                                                                                         >  2:1292725/1‑150 (MQ=255)
                          tcaatcCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGTTGAATATTTTTTCAGCGCCAtttttatt                                                                                                                         <  2:688002/150‑8 (MQ=255)
                          tcaatcCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGTTGAATATTTTTTCAGCGCCAtttttatt                                                                                                                         <  2:406896/150‑8 (MQ=255)
                          tcaatcCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGTTGAATATATTTTCAGCGCCAtttttatt                                                                                                                         <  1:778412/150‑8 (MQ=255)
                          tcaatcCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAGCGCCATCgcgttt                                                                                                                         >  2:514385/1‑145 (MQ=255)
                             atcCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTa                                                                                                                      >  2:148797/1‑150 (MQ=255)
                             atcCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTa                                                                                                                      >  1:669362/1‑150 (MQ=255)
                               caaGACCATATGTGTCCGACGATTTTACGCCCTGTCATCCTGTTAGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATTGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATg                                                                                                                    <  2:1283835/148‑1 (MQ=255)
                                   aCCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCtt                                                                                                                >  2:1121325/1‑150 (MQ=255)
                                     cATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTg                                                                                                              >  2:1132555/1‑150 (MQ=255)
                                      atatGTGTCCCACGATTTTAAGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGc                                                                                                             >  1:670518/1‑150 (MQ=255)
                                        atGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCgg                                                                                                           >  2:236316/1‑150 (MQ=255)
                                            gtCCCCCGATTTTACGCCCTGTCATCCTGTTCGGGGCTCATTCTAACAGAAAAAGAAACCGTTTGCGTAGGGAGTTCCTTCCCGCGCATCAATAAAACTGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATc                                                                                                       <  1:217577/150‑1 (MQ=255)
                                            gtCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCTTc                                                                                                       >  2:1119915/1‑150 (MQ=255)
                                                    aTTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATg                                                                                               >  1:1274427/1‑150 (MQ=255)
                                                    aTTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGCGATTTCCTTCCCTCGCATCAATAAAAATGGCGCTGAAAAAATATTAAACGCCATCGACTTTTTATGACTTTGAGGCATCGGGCAATg                                                                                               >  2:1220437/1‑150 (MQ=255)
                                                     ttttACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGc                                                                                              <  1:348563/150‑1 (MQ=255)
                                                       ttACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCgt                                                                                            <  1:381000/150‑1 (MQ=255)
                                                          cGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTc                                                                                         <  1:329946/150‑1 (MQ=255)
                                                           gCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCg                                                                                        >  1:700103/1‑150 (MQ=255)
                                                           gCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCg                                                                                        >  1:900602/1‑150 (MQ=255)
                                                            cccTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCgg                                                                                       >  1:516209/1‑150 (MQ=255)
                                                            cccTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCgg                                                                                       <  1:308401/150‑1 (MQ=255)
                                                                  cATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATgcgg                                                                                 <  1:951537/150‑1 (MQ=255)
                                                                   aTCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTGAACGCCATCGACTTTTTATGCCATTGCGGCATCGGGCAATGCGTGTCGGATgcggc                                                                                >  2:359444/1‑150 (MQ=255)
                                                                   aTCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATgcggc                                                                                >  2:138355/1‑150 (MQ=255)
                                                                    tCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGCATTCCTTCCCGCGCATCAATAAAAATGGCGCGGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGGCGGATgcggcg                                                                               <  2:53386/150‑1 (MQ=255)
                                                                          gcGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACg                                                                         <  1:260178/149‑1 (MQ=255)
                                                                            ggggCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGcc                                                                       <  1:1074013/150‑1 (MQ=255)
                                                                              ggCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCtt                                                                     >  2:929568/1‑150 (MQ=255)
                                                                              ggCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGATGAAAAAATATTCAACGCAATCGACTTTTTATGCATTTTCGGCATCGGGCAATGCGTGAAGGATTCGTCGTAAActactt                                                                     >  1:1138810/1‑145 (MQ=255)
                                                                                cgcATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTAt                                                                   >  1:1238216/1‑150 (MQ=255)
                                                                                  cATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATcc                                                                 >  2:524692/1‑150 (MQ=255)
                                                                                      cTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGAcc                                                             <  1:486370/150‑1 (MQ=255)
                                                                                          cAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACg                                                         >  2:623086/1‑150 (MQ=255)
                                                                                          cAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACg                                                         >  1:1316346/1‑150 (MQ=255)
                                                                                          cAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCT‑‑TCCGACCTACGGt                                                       >  2:241267/1‑150 (MQ=255)
                                                                                            gaaaaagaaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGt                                                       >  1:1014968/1‑150 (MQ=255)
                                                                                                   aaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTAccc                                                >  1:299607/1‑150 (MQ=255)
                                                                                                    aaaCTTTTGCGTATGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTAcccc                                               <  2:373545/150‑1 (MQ=255)
                                                                                                      aCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTCCCCCTg                                             >  1:528995/1‑150 (MQ=255)
                                                                                                             cGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGc                                      >  2:271102/1‑150 (MQ=255)
                                                                                                               tAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCt                                    <  1:863481/150‑1 (MQ=255)
                                                                                                                aGGGATTTCCTTCCCGCGCATCAATAAAGATGGCGCTGAAAAAATAGTCCACGCCGTCGACGTTTTATGCCTTTGCGGCAGCGGGCGATGCGTGTCGGATGCGGCGTCTACGCCGTCTCCGACGTCCGGTTCTGCCGCTGGGTAGGccgg                                   >  2:1219965/1‑148 (MQ=255)
                                                                                                                  ggATTTCCTTACCTAGCATCAATAAAAATGTAGCTGAAAAAATATTCAACTCCATCGACTTTTTATGCATTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGAt                                 <  2:184459/150‑1 (MQ=38)
                                                                                                                   gATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATa                                >  2:648751/1‑150 (MQ=255)
                                                                                                                       tccttccCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAgcc                            >  2:683807/1‑148 (MQ=255)
                                                                                                                        ccttccCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATATACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGTATGCTTCGTAAACGCGTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGAcg                           <  1:1015421/150‑1 (MQ=255)
                                                                                                                              cgcgCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAg                     <  1:700973/150‑1 (MQ=255)
                                                                                                                              cgcgCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAg                     >  1:331235/1‑150 (MQ=255)
                                                                                                                                    tCAATAAAAATGGCGCGGAAAAAATATTCACCGCCATCGACTTTTTCTGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGc               <  1:533352/150‑1 (MQ=255)
                                                                                                                                     cAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCa              <  2:1280415/150‑1 (MQ=255)
                                                                                                                                      aaTAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCAt             >  1:207002/1‑150 (MQ=255)
                                                                                                                                      aaTAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACACCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCAt             >  2:526183/1‑150 (MQ=255)
                                                                                                                                       aTAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATc            <  1:1266337/150‑1 (MQ=255)
                                                                                                                                          tcgaTGGCGTTGAATATTTTTTCAGCGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAgg         >  2:914102/4‑150 (MQ=37)
                                                                                                                                          aaaaTGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAgg         >  2:594363/1‑150 (MQ=255)
                                                                                                                                           cgaTGGCGTTGAATATTTTTTCAGCGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCATATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGc        >  1:54904/3‑150 (MQ=37)
                                                                                                                                            aaTGTCGCTGAAAAAATATTCAACTCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCa       <  2:822317/150‑1 (MQ=255)
                                                                                                                                             aaggCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCaa      <  2:555370/148‑1 (MQ=255)
                                                                                                                                              tGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAg     <  2:1274427/150‑1 (MQ=255)
                                                                                                                                                gcgcTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGAc   >  1:1185899/1‑150 (MQ=255)
                                                                                                                                                 cgcTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCTTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATATGACGCGCCAGCGTCGCATCAGGCAAGAcg  <  1:1004993/150‑2 (MQ=255)
                                                                                                                                                 cgcTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGAcc  >  1:173679/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AAGCAATCACCGCGATTATGGCGTAATCAATCCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACC  >  NC_000913/2430724‑2431018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: