Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,430,876 A→T 7.9% intergenic (‑113/+146) cvpA ← / ← dedD colicin V production protein/membrane‑anchored periplasmic protein involved in septation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,430,8760AT 7.9% 203.7 / 7.3 76intergenic (‑113/+146)cvpA/dedDcolicin V production protein/membrane‑anchored periplasmic protein involved in septation
Reads supporting (aligned to +/- strand):  ref base A (44/26);  new base T (2/4);  total (46/30)
Fisher's exact test for biased strand distribution p-value = 2.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.36e-01

CAATCACCGCGATTATGGCGTAATCAATCCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTA  >  NC_000913/2430727‑2431024
                                                                                                                                                     |                                                                                                                                                    
cAATCACCGCGATTATGGCGTAATCAATCCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGaaa                                                                                                                                                      >  2:810109/1‑150 (MQ=255)
   acACCGCGATTATCGCGTAATCAATCCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGaaaaaa                                                                                                                                                   <  2:1184004/149‑1 (MQ=255)
    cACCGCGATTATGGCGTAATCAATCCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCTCTGAAAAAat                                                                                                                                                  >  1:373545/1‑150 (MQ=255)
    cACCGCGATTATGGCGTAATCAATCCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAat                                                                                                                                                  <  2:270216/150‑1 (MQ=255)
     aCCGCGCTTATTGCGTTATCAATCCAGACAATATGTGTCCCAAGATTTTACTCCCTGTCATCGTGTTCGGGTCGCTTTCTAACAGAATAAGAAAACGTTTGCGTAGGGATTGCCTTCCCGGGCATGAATAAAAATGGAGCTGaataaata                                                                                                                                                 <  1:400487/150‑1 (MQ=255)
      ccGCGATTATGGCGTAATCAATCCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAatat                                                                                                                                                >  2:1061984/1‑150 (MQ=255)
       cgcgATTATGGCGTAATCAATCCAGACCATATGTGTCCCACGATTTTACGACATGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATAtt                                                                                                                                               >  2:584985/1‑150 (MQ=255)
         cgATTATGGCGTAATCAATCCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCa                                                                                                                                             <  1:1086728/150‑1 (MQ=255)
               tGGCGTAATCAATCCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCa                                                                                                                                       >  1:67131/1‑150 (MQ=255)
               tGGCGTAATCAATCCAGACCATATGTGTCCCACGATTTTACGCACTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCa                                                                                                                                       >  2:928804/1‑150 (MQ=255)
                ggCGTAATCAATCCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGTTGAATATTTTTTCAGCGCCAt                                                                                                                                      <  1:130915/150‑1 (MQ=255)
                  cGTAATCAATCCAGCCCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGTGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCAtag                                                                                                                                    >  2:1143048/1‑148 (MQ=255)
                    tAATCAATCCAGACCATATGTGTCCCACGATTTTACGCCATGTCATCCTGTTCGGGGCGCATTCTAACAGAAAATGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGTAAAAATATTCAACGCGATCGAc                                                                                                                                  >  1:655599/1‑150 (MQ=255)
                      atcaatcCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACtt                                                                                                                                >  2:1266337/1‑150 (MQ=255)
                       tcaatcCAGACCTTATGTGTACCACGATTTTACGCCCTGTCATCCTGTTCGGGTCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCTCTGAAAAAATATTCAACGCCATCGACttt                                                                                                                               <  1:883684/150‑1 (MQ=255)
                       tcaatcCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGCTTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATTTTCAACGCCGTCGACgtt                                                                                                                               >  2:1292725/1‑150 (MQ=255)
                       tcaatcCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGTTGAATATTTTTTCAGCGCCAtttttatt                                                                                                                               <  2:406896/150‑8 (MQ=255)
                       tcaatcCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGTTGAATATTTTTTCAGCGCCAtttttatt                                                                                                                               <  2:688002/150‑8 (MQ=255)
                       tcaatcCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGTTGAATATATTTTCAGCGCCAtttttatt                                                                                                                               <  1:778412/150‑8 (MQ=255)
                       tcaatcCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAGCGCCATCgcgttt                                                                                                                               >  2:514385/1‑145 (MQ=255)
                          atcCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTa                                                                                                                            >  2:148797/1‑150 (MQ=255)
                          atcCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTa                                                                                                                            >  1:669362/1‑150 (MQ=255)
                            caaGACCATATGTGTCCGACGATTTTACGCCCTGTCATCCTGTTAGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATTGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATg                                                                                                                          <  2:1283835/148‑1 (MQ=255)
                                aCCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCtt                                                                                                                      >  2:1121325/1‑150 (MQ=255)
                                  cATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTg                                                                                                                    >  2:1132555/1‑150 (MQ=255)
                                   atatGTGTCCCACGATTTTAAGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGc                                                                                                                   >  1:670518/1‑150 (MQ=255)
                                     atGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCgg                                                                                                                 >  2:236316/1‑150 (MQ=255)
                                         gtCCCCCGATTTTACGCCCTGTCATCCTGTTCGGGGCTCATTCTAACAGAAAAAGAAACCGTTTGCGTAGGGAGTTCCTTCCCGCGCATCAATAAAACTGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATc                                                                                                             <  1:217577/150‑1 (MQ=255)
                                         gtCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCTTc                                                                                                             >  2:1119915/1‑150 (MQ=255)
                                                 aTTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATg                                                                                                     >  1:1274427/1‑150 (MQ=255)
                                                 aTTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGCGATTTCCTTCCCTCGCATCAATAAAAATGGCGCTGAAAAAATATTAAACGCCATCGACTTTTTATGACTTTGAGGCATCGGGCAATg                                                                                                     >  2:1220437/1‑150 (MQ=255)
                                                  ttttACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGc                                                                                                    <  1:348563/150‑1 (MQ=255)
                                                    ttACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCgt                                                                                                  <  1:381000/150‑1 (MQ=255)
                                                       cGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTc                                                                                               <  1:329946/150‑1 (MQ=255)
                                                        gCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCg                                                                                              >  1:700103/1‑150 (MQ=255)
                                                        gCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCg                                                                                              >  1:900602/1‑150 (MQ=255)
                                                         cccTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCgg                                                                                             <  1:308401/150‑1 (MQ=255)
                                                         cccTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCgg                                                                                             >  1:516209/1‑150 (MQ=255)
                                                               cATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATgcgg                                                                                       <  1:951537/150‑1 (MQ=255)
                                                                aTCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTGAACGCCATCGACTTTTTATGCCATTGCGGCATCGGGCAATGCGTGTCGGATgcggc                                                                                      >  2:359444/1‑150 (MQ=255)
                                                                aTCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATgcggc                                                                                      >  2:138355/1‑150 (MQ=255)
                                                                 tCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGCATTCCTTCCCGCGCATCAATAAAAATGGCGCGGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGGCGGATgcggcg                                                                                     <  2:53386/150‑1 (MQ=255)
                                                                       gcGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACg                                                                               <  1:260178/149‑1 (MQ=255)
                                                                         ggggCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGcc                                                                             <  1:1074013/150‑1 (MQ=255)
                                                                           ggCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCtt                                                                           >  2:929568/1‑150 (MQ=255)
                                                                           ggCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGATGAAAAAATATTCAACGCAATCGACTTTTTATGCATTTTCGGCATCGGGCAATGCGTGAAGGATTCGTCGTAAActactt                                                                           >  1:1138810/1‑145 (MQ=255)
                                                                             cgcATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTAt                                                                         >  1:1238216/1‑150 (MQ=255)
                                                                               cATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATcc                                                                       >  2:524692/1‑150 (MQ=255)
                                                                                   cTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGAcc                                                                   <  1:486370/150‑1 (MQ=255)
                                                                                       cAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACg                                                               >  2:623086/1‑150 (MQ=255)
                                                                                       cAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACg                                                               >  1:1316346/1‑150 (MQ=255)
                                                                                       cAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCT‑‑TCCGACCTACGGt                                                             >  2:241267/1‑150 (MQ=255)
                                                                                         gaaaaagaaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGt                                                             >  1:1014968/1‑150 (MQ=255)
                                                                                                aaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTAccc                                                      >  1:299607/1‑150 (MQ=255)
                                                                                                 aaaCTTTTGCGTATGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTAcccc                                                     <  2:373545/150‑1 (MQ=255)
                                                                                                   aCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTCCCCCTg                                                   >  1:528995/1‑150 (MQ=255)
                                                                                                          cGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGc                                            >  2:271102/1‑150 (MQ=255)
                                                                                                            tAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCt                                          <  1:863481/150‑1 (MQ=255)
                                                                                                             aGGGATTTCCTTCCCGCGCATCAATAAAGATGGCGCTGAAAAAATAGTCCACGCCGTCGACGTTTTATGCCTTTGCGGCAGCGGGCGATGCGTGTCGGATGCGGCGTCTACGCCGTCTCCGACGTCCGGTTCTGCCGCTGGGTAGGccgg                                         >  2:1219965/1‑148 (MQ=255)
                                                                                                               ggATTTCCTTACCTAGCATCAATAAAAATGTAGCTGAAAAAATATTCAACTCCATCGACTTTTTATGCATTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGAt                                       <  2:184459/150‑1 (MQ=38)
                                                                                                                gATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATa                                      >  2:648751/1‑150 (MQ=255)
                                                                                                                    tccttccCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAgcc                                  >  2:683807/1‑148 (MQ=255)
                                                                                                                     ccttccCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATATACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGTATGCTTCGTAAACGCGTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGAcg                                 <  1:1015421/150‑1 (MQ=255)
                                                                                                                           cgcgCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAg                           >  1:331235/1‑150 (MQ=255)
                                                                                                                           cgcgCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAg                           <  1:700973/150‑1 (MQ=255)
                                                                                                                                 tCAATAAAAATGGCGCGGAAAAAATATTCACCGCCATCGACTTTTTCTGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGc                     <  1:533352/150‑1 (MQ=255)
                                                                                                                                  cAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCa                    <  2:1280415/150‑1 (MQ=255)
                                                                                                                                   aaTAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCAt                   >  1:207002/1‑150 (MQ=255)
                                                                                                                                   aaTAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACACCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCAt                   >  2:526183/1‑150 (MQ=255)
                                                                                                                                    aTAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATc                  <  1:1266337/150‑1 (MQ=255)
                                                                                                                                       tcgaTGGCGTTGAATATTTTTTCAGCGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAgg               >  2:914102/4‑150 (MQ=37)
                                                                                                                                       aaaaTGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAgg               >  2:594363/1‑150 (MQ=255)
                                                                                                                                        cgaTGGCGTTGAATATTTTTTCAGCGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCATATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGc              >  1:54904/3‑150 (MQ=37)
                                                                                                                                         aaTGTCGCTGAAAAAATATTCAACTCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCa             <  2:822317/150‑1 (MQ=255)
                                                                                                                                          aaggCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCaa            <  2:555370/148‑1 (MQ=255)
                                                                                                                                           tGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAg           <  2:1274427/150‑1 (MQ=255)
                                                                                                                                             gcgcTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGAc         >  1:1185899/1‑150 (MQ=255)
                                                                                                                                              cgcTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCTTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATATGACGCGCCAGCGTCGCATCAGGCAAGAcg        <  1:1004993/150‑2 (MQ=255)
                                                                                                                                              cgcTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGAcc        >  1:173679/1‑150 (MQ=255)
                                                                                                                                                    aaaaaTATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTa  >  1:1075642/1‑150 (MQ=255)
                                                                                                                                                    aaaaaTATTCAACGCCATCGACTTTTTATGCCAATGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGTCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTa  >  1:775700/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CAATCACCGCGATTATGGCGTAATCAATCCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTA  >  NC_000913/2430727‑2431024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: