Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,430,878 A→T 7.8% intergenic (‑115/+144) cvpA ← / ← dedD colicin V production protein/membrane‑anchored periplasmic protein involved in septation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,430,8780AT 7.8% 204.7 / 5.7 76intergenic (‑115/+144)cvpA/dedDcolicin V production protein/membrane‑anchored periplasmic protein involved in septation
Reads supporting (aligned to +/- strand):  ref base A (44/26);  new base T (2/4);  total (46/30)
Fisher's exact test for biased strand distribution p-value = 2.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.66e-01

TCACCGCGATTATGGCGTAATCAATCCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTA  >  NC_000913/2430730‑2431024
                                                                                                                                                    |                                                                                                                                                  
acACCGCGATTATCGCGTAATCAATCCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGaaaaaa                                                                                                                                                   <  2:1184004/149‑1 (MQ=255)
 cACCGCGATTATGGCGTAATCAATCCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCTCTGAAAAAat                                                                                                                                                  >  1:373545/1‑150 (MQ=255)
 cACCGCGATTATGGCGTAATCAATCCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAat                                                                                                                                                  <  2:270216/150‑1 (MQ=255)
  aCCGCGCTTATTGCGTTATCAATCCAGACAATATGTGTCCCAAGATTTTACTCCCTGTCATCGTGTTCGGGTCGCTTTCTAACAGAATAAGAAAACGTTTGCGTAGGGATTGCCTTCCCGGGCATGAATAAAAATGGAGCTGaataaata                                                                                                                                                 <  1:400487/150‑1 (MQ=255)
   ccGCGATTATGGCGTAATCAATCCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAatat                                                                                                                                                >  2:1061984/1‑150 (MQ=255)
    cgcgATTATGGCGTAATCAATCCAGACCATATGTGTCCCACGATTTTACGACATGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATAtt                                                                                                                                               >  2:584985/1‑150 (MQ=255)
      cgATTATGGCGTAATCAATCCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCa                                                                                                                                             <  1:1086728/150‑1 (MQ=255)
            tGGCGTAATCAATCCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCa                                                                                                                                       >  1:67131/1‑150 (MQ=255)
            tGGCGTAATCAATCCAGACCATATGTGTCCCACGATTTTACGCACTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCa                                                                                                                                       >  2:928804/1‑150 (MQ=255)
             ggCGTAATCAATCCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGTTGAATATTTTTTCAGCGCCAt                                                                                                                                      <  1:130915/150‑1 (MQ=255)
               cGTAATCAATCCAGCCCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGTGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCAtag                                                                                                                                    >  2:1143048/1‑148 (MQ=255)
                 tAATCAATCCAGACCATATGTGTCCCACGATTTTACGCCATGTCATCCTGTTCGGGGCGCATTCTAACAGAAAATGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGTAAAAATATTCAACGCGATCGAc                                                                                                                                  >  1:655599/1‑150 (MQ=255)
                   atcaatcCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACtt                                                                                                                                >  2:1266337/1‑150 (MQ=255)
                    tcaatcCAGACCTTATGTGTACCACGATTTTACGCCCTGTCATCCTGTTCGGGTCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCTCTGAAAAAATATTCAACGCCATCGACttt                                                                                                                               <  1:883684/150‑1 (MQ=255)
                    tcaatcCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGCTTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATTTTCAACGCCGTCGACgtt                                                                                                                               >  2:1292725/1‑150 (MQ=255)
                    tcaatcCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGTTGAATATTTTTTCAGCGCCAtttttatt                                                                                                                               <  2:406896/150‑8 (MQ=255)
                    tcaatcCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGTTGAATATTTTTTCAGCGCCAtttttatt                                                                                                                               <  2:688002/150‑8 (MQ=255)
                    tcaatcCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGTTGAATATATTTTCAGCGCCAtttttatt                                                                                                                               <  1:778412/150‑8 (MQ=255)
                    tcaatcCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAGCGCCATCgcgttt                                                                                                                               >  2:514385/1‑145 (MQ=255)
                       atcCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTa                                                                                                                            >  2:148797/1‑150 (MQ=255)
                       atcCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTa                                                                                                                            >  1:669362/1‑150 (MQ=255)
                         caaGACCATATGTGTCCGACGATTTTACGCCCTGTCATCCTGTTAGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATTGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATg                                                                                                                          <  2:1283835/148‑1 (MQ=255)
                             aCCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCtt                                                                                                                      >  2:1121325/1‑150 (MQ=255)
                               cATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTg                                                                                                                    >  2:1132555/1‑150 (MQ=255)
                                atatGTGTCCCACGATTTTAAGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGc                                                                                                                   >  1:670518/1‑150 (MQ=255)
                                  atGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCgg                                                                                                                 >  2:236316/1‑150 (MQ=255)
                                      gtCCCCCGATTTTACGCCCTGTCATCCTGTTCGGGGCTCATTCTAACAGAAAAAGAAACCGTTTGCGTAGGGAGTTCCTTCCCGCGCATCAATAAAACTGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATc                                                                                                             <  1:217577/150‑1 (MQ=255)
                                      gtCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCTTc                                                                                                             >  2:1119915/1‑150 (MQ=255)
                                              aTTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATg                                                                                                     >  1:1274427/1‑150 (MQ=255)
                                              aTTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGCGATTTCCTTCCCTCGCATCAATAAAAATGGCGCTGAAAAAATATTAAACGCCATCGACTTTTTATGACTTTGAGGCATCGGGCAATg                                                                                                     >  2:1220437/1‑150 (MQ=255)
                                               ttttACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGc                                                                                                    <  1:348563/150‑1 (MQ=255)
                                                 ttACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCgt                                                                                                  <  1:381000/150‑1 (MQ=255)
                                                    cGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTc                                                                                               <  1:329946/150‑1 (MQ=255)
                                                     gCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCg                                                                                              >  1:700103/1‑150 (MQ=255)
                                                     gCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCg                                                                                              >  1:900602/1‑150 (MQ=255)
                                                      cccTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCgg                                                                                             >  1:516209/1‑150 (MQ=255)
                                                      cccTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCgg                                                                                             <  1:308401/150‑1 (MQ=255)
                                                            cATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATgcgg                                                                                       <  1:951537/150‑1 (MQ=255)
                                                             aTCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTGAACGCCATCGACTTTTTATGCCATTGCGGCATCGGGCAATGCGTGTCGGATgcggc                                                                                      >  2:359444/1‑150 (MQ=255)
                                                             aTCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATgcggc                                                                                      >  2:138355/1‑150 (MQ=255)
                                                              tCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGCATTCCTTCCCGCGCATCAATAAAAATGGCGCGGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGGCGGATgcggcg                                                                                     <  2:53386/150‑1 (MQ=255)
                                                                    gcGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACg                                                                               <  1:260178/149‑1 (MQ=255)
                                                                      ggggCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGcc                                                                             <  1:1074013/150‑1 (MQ=255)
                                                                        ggCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCtt                                                                           >  2:929568/1‑150 (MQ=255)
                                                                        ggCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGATGAAAAAATATTCAACGCAATCGACTTTTTATGCATTTTCGGCATCGGGCAATGCGTGAAGGATTCGTCGTAAActactt                                                                           >  1:1138810/1‑145 (MQ=255)
                                                                          cgcATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTAt                                                                         >  1:1238216/1‑150 (MQ=255)
                                                                            cATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATcc                                                                       >  2:524692/1‑150 (MQ=255)
                                                                                cTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGAcc                                                                   <  1:486370/150‑1 (MQ=255)
                                                                                    cAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACg                                                               >  2:623086/1‑150 (MQ=255)
                                                                                    cAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACg                                                               >  1:1316346/1‑150 (MQ=255)
                                                                                    cAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCT‑‑TCCGACCTACGGt                                                             >  2:241267/1‑150 (MQ=255)
                                                                                      gaaaaagaaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGt                                                             >  1:1014968/1‑150 (MQ=255)
                                                                                             aaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTAccc                                                      >  1:299607/1‑150 (MQ=255)
                                                                                              aaaCTTTTGCGTATGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTAcccc                                                     <  2:373545/150‑1 (MQ=255)
                                                                                                aCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTCCCCCTg                                                   >  1:528995/1‑150 (MQ=255)
                                                                                                       cGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGc                                            >  2:271102/1‑150 (MQ=255)
                                                                                                         tAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCt                                          <  1:863481/150‑1 (MQ=255)
                                                                                                          aGGGATTTCCTTCCCGCGCATCAATAAAGATGGCGCTGAAAAAATAGTCCACGCCGTCGACGTTTTATGCCTTTGCGGCAGCGGGCGATGCGTGTCGGATGCGGCGTCTACGCCGTCTCCGACGTCCGGTTCTGCCGCTGGGTAGGccgg                                         >  2:1219965/1‑148 (MQ=255)
                                                                                                            ggATTTCCTTACCTAGCATCAATAAAAATGTAGCTGAAAAAATATTCAACTCCATCGACTTTTTATGCATTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGAt                                       <  2:184459/150‑1 (MQ=38)
                                                                                                             gATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATa                                      >  2:648751/1‑150 (MQ=255)
                                                                                                                 tccttccCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAgcc                                  >  2:683807/1‑148 (MQ=255)
                                                                                                                  ccttccCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATATACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGTATGCTTCGTAAACGCGTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGAcg                                 <  1:1015421/150‑1 (MQ=255)
                                                                                                                        cgcgCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAg                           >  1:331235/1‑150 (MQ=255)
                                                                                                                        cgcgCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAg                           <  1:700973/150‑1 (MQ=255)
                                                                                                                              tCAATAAAAATGGCGCGGAAAAAATATTCACCGCCATCGACTTTTTCTGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGc                     <  1:533352/150‑1 (MQ=255)
                                                                                                                               cAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCa                    <  2:1280415/150‑1 (MQ=255)
                                                                                                                                aaTAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCAt                   >  1:207002/1‑150 (MQ=255)
                                                                                                                                aaTAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACACCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCAt                   >  2:526183/1‑150 (MQ=255)
                                                                                                                                 aTAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATc                  <  1:1266337/150‑1 (MQ=255)
                                                                                                                                    tcgaTGGCGTTGAATATTTTTTCAGCGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAgg               >  2:914102/4‑150 (MQ=37)
                                                                                                                                    aaaaTGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAgg               >  2:594363/1‑150 (MQ=255)
                                                                                                                                     cgaTGGCGTTGAATATTTTTTCAGCGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCATATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGc              >  1:54904/3‑150 (MQ=37)
                                                                                                                                      aaTGTCGCTGAAAAAATATTCAACTCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCa             <  2:822317/150‑1 (MQ=255)
                                                                                                                                       aaggCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCaa            <  2:555370/148‑1 (MQ=255)
                                                                                                                                        tGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAg           <  2:1274427/150‑1 (MQ=255)
                                                                                                                                          gcgcTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGAc         >  1:1185899/1‑150 (MQ=255)
                                                                                                                                           cgcTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCTTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATATGACGCGCCAGCGTCGCATCAGGCAAGAcg        <  1:1004993/150‑2 (MQ=255)
                                                                                                                                           cgcTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGAcc        >  1:173679/1‑150 (MQ=255)
                                                                                                                                                 aaaaaTATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTa  >  1:1075642/1‑150 (MQ=255)
                                                                                                                                                 aaaaaTATTCAACGCCATCGACTTTTTATGCCAATGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGTCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTa  >  1:775700/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TCACCGCGATTATGGCGTAATCAATCCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTA  >  NC_000913/2430730‑2431024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: