Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 237,410 G→C 5.4% D26H (GAT→CAT)  yafT → lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913237,4100GC 5.4% 271.1 / 4.6 87D26H (GAT→CAT) yafTlipoprotein
Reads supporting (aligned to +/- strand):  ref base G (40/41);  new base C (2/3);  total (42/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTC  >  NC_000913/237261‑237559
                                                                                                                                                     |                                                                                                                                                     
tGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATtg                                                                                                                                                       <  2:985174/150‑1 (MQ=255)
 gAGGTATATACTTAGAGTCCGTTATCAAAGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATtga                                                                                                                                                      <  2:635796/150‑1 (MQ=255)
 gAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGACTTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATtga                                                                                                                                                      <  1:187950/150‑1 (MQ=255)
   ggTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATtgatg                                                                                                                                                    >  2:34972/1‑150 (MQ=255)
    gTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATtgatga                                                                                                                                                   >  1:353747/1‑150 (MQ=255)
      atataATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGaaa                                                                                                                                                 >  1:1213594/1‑150 (MQ=255)
         taATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAaaga                                                                                                                                              >  2:314741/1‑150 (MQ=255)
          aaTTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAaagaa                                                                                                                                             <  2:514732/150‑1 (MQ=255)
              agagTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGaaa                                                                                                                                         <  1:168173/150‑1 (MQ=255)
                agTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCAAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGaaaaa                                                                                                                                       <  2:611196/150‑1 (MQ=255)
                 gTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGATTGTCCAATTATGAATTCAAAATAGCTTTGGTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTTTTCATCAATagaagattcag                                                                                                                                      <  2:954826/150‑6 (MQ=255)
                 gTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTGCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTTTTCATCAATagaagattcag                                                                                                                                      <  2:954825/150‑6 (MQ=255)
                 gTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTTTTCATCAATagaagattcag                                                                                                                                      <  1:1115333/150‑6 (MQ=255)
                   ccGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGc                                                                                                                                    <  1:1269244/150‑1 (MQ=255)
                     gTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTc                                                                                                                                  >  1:1147712/1‑150 (MQ=255)
                      ttATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCa                                                                                                                                 <  2:43116/150‑1 (MQ=255)
                      ttATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGGGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCa                                                                                                                                 <  2:51701/150‑1 (MQ=255)
                        aTCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGCATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAg                                                                                                                               >  2:192358/1‑150 (MQ=255)
                         tCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGt                                                                                                                              >  2:113352/1‑150 (MQ=255)
                          cAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTc                                                                                                                             <  2:499075/150‑1 (MQ=255)
                               cTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTcacaca                                                                                                                        <  1:381706/150‑1 (MQ=255)
                                tAAATATTCTAATCATTATGACAGGCGAGGGACTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATTAAAAGAAGAAAAAAGCTCAAGTCACACaa                                                                                                                       >  1:172666/1‑150 (MQ=255)
                                   atatTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGACTTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGt                                                                                                                    <  2:263079/150‑1 (MQ=255)
                                   atatTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGt                                                                                                                    <  1:834261/150‑1 (MQ=255)
                                     atTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTaa                                                                                                                  <  1:1138842/150‑1 (MQ=255)
                                          aaTCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATAAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATta                                                                                                             >  1:252720/1‑150 (MQ=255)
                                             cATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATtaata                                                                                                          >  2:260512/1‑150 (MQ=255)
                                              aTTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATtaataa                                                                                                         <  2:1127576/150‑1 (MQ=255)
                                                  tGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAac                                                                                                     <  1:665162/150‑1 (MQ=255)
                                                  tGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAac                                                                                                     <  1:448729/150‑1 (MQ=255)
                                                       ggCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTcc                                                                                                <  1:625459/150‑1 (MQ=255)
                                                       ggCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTcc                                                                                                <  1:1256192/150‑1 (MQ=255)
                                                       agCGAGGGAGTGTACAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTcc                                                                                                <  2:442588/149‑1 (MQ=255)
                                                        gCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTccc                                                                                               >  2:594858/1‑150 (MQ=255)
                                                          gAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGGAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCa                                                                                             <  2:1060626/150‑1 (MQ=255)
                                                           aGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGATGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAg                                                                                            >  1:930330/1‑150 (MQ=255)
                                                           aGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCAGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTTATAAAAAAACTCCCAAg                                                                                            >  1:504341/1‑150 (MQ=255)
                                                            gggAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGc                                                                                           <  2:644324/150‑1 (MQ=255)
                                                                 tgtCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTg                                                                                      >  1:324865/1‑150 (MQ=255)
                                                                 tgtCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTg                                                                                      >  1:1252032/1‑150 (MQ=255)
                                                                     cAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTgacag                                                                                  >  2:339794/1‑150 (MQ=255)
                                                                            gAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGa                                                                           >  1:39979/1‑150 (MQ=255)
                                                                             aaTTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGAt                                                                          >  1:628332/1‑150 (MQ=255)
                                                                             aaTTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGAt                                                                          >  1:1085464/1‑150 (MQ=255)
                                                                               ttCAAAAAAGCTTTTTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATATTCTATTCATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATATAAACACTAACCAGCAACTGACAGACAAAGAttt                                                                        >  1:917494/1‑150 (MQ=255)
                                                                                 cAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTAt                                                                      >  1:268139/1‑150 (MQ=255)
                                                                                 cAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTAt                                                                      >  1:1069856/1‑150 (MQ=255)
                                                                                  aaaaaaGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTAtt                                                                     >  2:1105879/1‑150 (MQ=255)
                                                                                   aaaaaGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTc                                                                    <  1:561663/150‑1 (MQ=255)
                                                                                   aaaaaGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTc                                                                    <  2:509917/150‑1 (MQ=255)
                                                                                        gCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTaa                                                               >  1:288245/1‑150 (MQ=255)
                                                                                          tttGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATg                                                             <  1:824709/150‑1 (MQ=255)
                                                                                          tttGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATg                                                             <  2:924790/150‑1 (MQ=255)
                                                                                            tgttgCATATTTGTGTTATTCTCGCTGCTTGCAGGATTTGACTCTGAATCTTCTATTTATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGaa                                                           <  1:666068/150‑1 (MQ=255)
                                                                                             gttgCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGaaa                                                          >  1:43714/1‑150 (MQ=255)
                                                                                              ttgCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAAc                                                         <  2:755417/150‑1 (MQ=255)
                                                                                              ttgCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAAc                                                         <  1:1047548/150‑1 (MQ=255)
                                                                                                     tgtgtgTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGAAACTGACAGAAAAATATTTATTCGGTAATCAAACCACTCTg                                                  >  2:210369/1‑150 (MQ=255)
                                                                                                       tgtgtTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGc                                                <  1:414383/150‑1 (MQ=255)
                                                                                                        gtgtTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGcc                                               >  1:565570/1‑150 (MQ=255)
                                                                                                         tgtTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAAACACTCTGGCCg                                              >  1:825679/1‑150 (MQ=255)
                                                                                                             aTTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACTAAGTAATATTAATAAAAACACTCCACAGAAACTGACAGACAAAGATTTATTCGGTAATGAAAAACCTCTGGCCGTATc                                          >  1:288094/1‑150 (MQ=255)
                                                                                                             aTTCTCGCTGCTTGCAGGATGTGCCTCTGAAGCTTCTATTGATGAAAACCAGAAAAAAGCTCAACTCACACAAATTAATATTAATAAAAACACTCCCCACCAACTGACACACAAAGATTAATTCGGTCCTGAAACCCCGCTGGCCGTATc                                          <  2:160775/150‑1 (MQ=255)
                                                                                                              ttCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATcc                                         <  2:347319/150‑1 (MQ=255)
                                                                                                                ctcGCTGCTTGCAGGATGTGCCTCTTAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACAAAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGa                                       <  2:150385/150‑1 (MQ=255)
                                                                                                                ctcGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGa                                       >  2:797023/1‑150 (MQ=255)
                                                                                                                ctcGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCAAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGa                                       >  2:974946/1‑150 (MQ=255)
                                                                                                                   gctgctTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAaga                                    >  1:1314704/1‑150 (MQ=255)
                                                                                                                   gctgctTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAaga                                    >  1:922875/1‑150 (MQ=255)
                                                                                                                       ctTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACAACTCTGGCCGTATCCGAAGAGGat                                >  1:1222853/1‑150 (MQ=255)
                                                                                                                        ttGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGata                               <  1:1033200/150‑1 (MQ=255)
                                                                                                                           cAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAATAAGATCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTAATTCGGGAATGAAACAACTCTGGCCGTATCCGAAGAGGATAATc                            >  2:757433/1‑150 (MQ=255)
                                                                                                                               aTGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGc                        >  1:1088167/1‑150 (MQ=255)
                                                                                                                               aTGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGAATTATTCGGTAATCAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGc                        >  1:1083064/1‑150 (MQ=255)
                                                                                                                                 gtgCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTg                      >  1:1223569/1‑150 (MQ=255)
                                                                                                                                    ccTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTgcgc                   >  2:759143/1‑150 (MQ=255)
                                                                                                                                     gcttttttcttctTTTCATCAATAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGAAACTGACAGACAATGATTTTTTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCt                  >  2:881259/8‑150 (MQ=255)
                                                                                                                                       ctGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCACAGCAACTCACAGACAAAGATTTATTCTGTAATTAAACCACTCTGGCCTAATCAGAAGAGGATATTAAAGCATCGCTTg                >  1:241829/1‑150 (MQ=255)
                                                                                                                                         gAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGAt              >  2:1029397/1‑150 (MQ=255)
                                                                                                                                          aaTCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATCTTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGACACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCGCAGCTTGaat             <  2:1190384/150‑3 (MQ=255)
                                                                                                                                           aTCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATgg            >  2:377449/1‑150 (MQ=255)
                                                                                                                                           aTCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATgg            >  2:1223534/1‑150 (MQ=255)
                                                                                                                                            tcttctATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGga           <  2:1123913/150‑1 (MQ=255)
                                                                                                                                            tcttctATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGga           <  1:178935/150‑1 (MQ=255)
                                                                                                                                            tcttctATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGga           <  2:1299210/150‑1 (MQ=255)
                                                                                                                                            tcttctATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGga           <  1:608457/150‑1 (MQ=255)
                                                                                                                                             cttctATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGgag          <  1:797023/150‑1 (MQ=255)
                                                                                                                                             cttctATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCGCAGCTTGaatatc          <  2:268139/150‑6 (MQ=255)
                                                                                                                                             attctATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGgag          <  1:675765/149‑1 (MQ=255)
                                                                                                                                              ttctATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCGCAGCTTGaatatcc         <  2:39979/150‑7 (MQ=255)
                                                                                                                                                ctATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATg       <  2:499357/150‑1 (MQ=255)
                                                                                                                                                 tATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGa      <  1:1091885/150‑1 (MQ=255)
                                                                                                                                                     gatgaAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTc  <  1:133653/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGAGGTATATAATTAGAGTCCGTTATCAATGCTAAATATTCTAATCATTATGACAGGCGAGGGAGTGTCCAATTATGAATTCAAAAAAGCTTTGTTGCATATGTGTGTTATTCTCGCTGCTTGCAGGATGTGCCTCTGAATCTTCTATTGATGAAAAGAAGAAAAAAGCTCAAGTCACACAAAGTAATATTAATAAAAACACTCCCCAGCAACTGACAGACAAAGATTTATTCGGTAATGAAACCACTCTGGCCGTATCCGAAGAGGATATTCAAGCTGCGCTTGATGGAGATGAGTTC  >  NC_000913/237261‑237559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑