Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,430,886 A→G 9.0% intergenic (‑123/+136) cvpA ← / ← dedD colicin V production protein/membrane‑anchored periplasmic protein involved in septation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,430,8860AG 9.0% 194.4 / 10.0 78intergenic (‑123/+136)cvpA/dedDcolicin V production protein/membrane‑anchored periplasmic protein involved in septation
Reads supporting (aligned to +/- strand):  ref base A (44/25);  new base G (3/4);  total (47/31)
Fisher's exact test for biased strand distribution p-value = 4.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.80e-01

TGGCGTAATCAATCCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTA  >  NC_000913/2430742‑2431034
                                                                                                                                                |                                                                                                                                                    
tGGCGTAATCAATCCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCa                                                                                                                                                 >  1:67131/1‑150 (MQ=255)
tGGCGTAATCAATCCAGACCATATGTGTCCCACGATTTTACGCACTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCa                                                                                                                                                 >  2:928804/1‑150 (MQ=255)
 ggCGTAATCAATCCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGTTGAATATTTTTTCAGCGCCAt                                                                                                                                                <  1:130915/150‑1 (MQ=255)
   cGTAATCAATCCAGCCCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGTGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCAtag                                                                                                                                              >  2:1143048/1‑148 (MQ=255)
     tAATCAATCCAGACCATATGTGTCCCACGATTTTACGCCATGTCATCCTGTTCGGGGCGCATTCTAACAGAAAATGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGTAAAAATATTCAACGCGATCGAc                                                                                                                                            >  1:655599/1‑150 (MQ=255)
       atcaatcCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACtt                                                                                                                                          >  2:1266337/1‑150 (MQ=255)
        tcaatcCAGACCTTATGTGTACCACGATTTTACGCCCTGTCATCCTGTTCGGGTCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCTCTGAAAAAATATTCAACGCCATCGACttt                                                                                                                                         <  1:883684/150‑1 (MQ=255)
        tcaatcCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGCTTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATTTTCAACGCCGTCGACgtt                                                                                                                                         >  2:1292725/1‑150 (MQ=255)
        tcaatcCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGTTGAATATTTTTTCAGCGCCAtttttatt                                                                                                                                         <  2:688002/150‑8 (MQ=255)
        tcaatcCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGTTGAATATTTTTTCAGCGCCAtttttatt                                                                                                                                         <  2:406896/150‑8 (MQ=255)
        tcaatcCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGTTGAATATATTTTCAGCGCCAtttttatt                                                                                                                                         <  1:778412/150‑8 (MQ=255)
        tcaatcCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAGCGCCATCgcgttt                                                                                                                                         >  2:514385/1‑145 (MQ=255)
           atcCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTa                                                                                                                                      >  2:148797/1‑150 (MQ=255)
           atcCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTa                                                                                                                                      >  1:669362/1‑150 (MQ=255)
             caaGACCATATGTGTCCGACGATTTTACGCCCTGTCATCCTGTTAGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATTGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATg                                                                                                                                    <  2:1283835/148‑1 (MQ=255)
                 aCCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCtt                                                                                                                                >  2:1121325/1‑150 (MQ=255)
                   cATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTg                                                                                                                              >  2:1132555/1‑150 (MQ=255)
                    atatGTGTCCCACGATTTTAAGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGc                                                                                                                             >  1:670518/1‑150 (MQ=255)
                      atGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCgg                                                                                                                           >  2:236316/1‑150 (MQ=255)
                          gtCCCCCGATTTTACGCCCTGTCATCCTGTTCGGGGCTCATTCTAACAGAAAAAGAAACCGTTTGCGTAGGGAGTTCCTTCCCGCGCATCAATAAAACTGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATc                                                                                                                       <  1:217577/150‑1 (MQ=255)
                          gtCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCTTc                                                                                                                       >  2:1119915/1‑150 (MQ=255)
                                  aTTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATg                                                                                                               >  1:1274427/1‑150 (MQ=255)
                                  aTTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGCGATTTCCTTCCCTCGCATCAATAAAAATGGCGCTGAAAAAATATTAAACGCCATCGACTTTTTATGACTTTGAGGCATCGGGCAATg                                                                                                               >  2:1220437/1‑150 (MQ=255)
                                   ttttACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGc                                                                                                              <  1:348563/150‑1 (MQ=255)
                                     ttACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCgt                                                                                                            <  1:381000/150‑1 (MQ=255)
                                        cGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTc                                                                                                         <  1:329946/150‑1 (MQ=255)
                                         gCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCg                                                                                                        >  1:900602/1‑150 (MQ=255)
                                         gCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCg                                                                                                        >  1:700103/1‑150 (MQ=255)
                                          cccTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCgg                                                                                                       >  1:516209/1‑150 (MQ=255)
                                          cccTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCgg                                                                                                       <  1:308401/150‑1 (MQ=255)
                                                cATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATgcgg                                                                                                 <  1:951537/150‑1 (MQ=255)
                                                 aTCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTGAACGCCATCGACTTTTTATGCCATTGCGGCATCGGGCAATGCGTGTCGGATgcggc                                                                                                >  2:359444/1‑150 (MQ=255)
                                                 aTCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATgcggc                                                                                                >  2:138355/1‑150 (MQ=255)
                                                  tCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGCATTCCTTCCCGCGCATCAATAAAAATGGCGCGGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGGCGGATgcggcg                                                                                               <  2:53386/150‑1 (MQ=255)
                                                        gcGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACg                                                                                         <  1:260178/149‑1 (MQ=255)
                                                          ggggCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGcc                                                                                       <  1:1074013/150‑1 (MQ=255)
                                                            ggCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCtt                                                                                     >  2:929568/1‑150 (MQ=255)
                                                            ggCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGATGAAAAAATATTCAACGCAATCGACTTTTTATGCATTTTCGGCATCGGGCAATGCGTGAAGGATTCGTCGTAAActactt                                                                                     >  1:1138810/1‑145 (MQ=255)
                                                              cgcATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTAt                                                                                   >  1:1238216/1‑150 (MQ=255)
                                                                cATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATcc                                                                                 >  2:524692/1‑150 (MQ=255)
                                                                    cTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGAcc                                                                             <  1:486370/150‑1 (MQ=255)
                                                                        cAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACg                                                                         >  2:623086/1‑150 (MQ=255)
                                                                        cAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACg                                                                         >  1:1316346/1‑150 (MQ=255)
                                                                        cAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCT‑‑TCCGACCTACGGt                                                                       >  2:241267/1‑150 (MQ=255)
                                                                          gaaaaagaaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGt                                                                       >  1:1014968/1‑150 (MQ=255)
                                                                                 aaaaCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTAccc                                                                >  1:299607/1‑150 (MQ=255)
                                                                                  aaaCTTTTGCGTATGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTAcccc                                                               <  2:373545/150‑1 (MQ=255)
                                                                                    aCGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTCCCCCTg                                                             >  1:528995/1‑150 (MQ=255)
                                                                                           cGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGc                                                      >  2:271102/1‑150 (MQ=255)
                                                                                             tAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCt                                                    <  1:863481/150‑1 (MQ=255)
                                                                                              aGGGATTTCCTTCCCGCGCATCAATAAAGATGGCGCTGAAAAAATAGTCCACGCCGTCGACGTTTTATGCCTTTGCGGCAGCGGGCGATGCGTGTCGGATGCGGCGTCTACGCCGTCTCCGACGTCCGGTTCTGCCGCTGGGTAGGccgg                                                   >  2:1219965/1‑148 (MQ=255)
                                                                                                ggATTTCCTTACCTAGCATCAATAAAAATGTAGCTGAAAAAATATTCAACTCCATCGACTTTTTATGCATTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGAt                                                 <  2:184459/150‑1 (MQ=38)
                                                                                                 gATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATa                                                >  2:648751/1‑150 (MQ=255)
                                                                                                     tccttccCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAgcc                                            >  2:683807/1‑148 (MQ=255)
                                                                                                      ccttccCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATATACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGTATGCTTCGTAAACGCGTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGAcg                                           <  1:1015421/150‑1 (MQ=255)
                                                                                                            cgcgCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAg                                     >  1:331235/1‑150 (MQ=255)
                                                                                                            cgcgCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAg                                     <  1:700973/150‑1 (MQ=255)
                                                                                                                  tCAATAAAAATGGCGCGGAAAAAATATTCACCGCCATCGACTTTTTCTGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGc                               <  1:533352/150‑1 (MQ=255)
                                                                                                                   cAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCa                              <  2:1280415/150‑1 (MQ=255)
                                                                                                                    aaTAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCAt                             >  1:207002/1‑150 (MQ=255)
                                                                                                                    aaTAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACACCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCAt                             >  2:526183/1‑150 (MQ=255)
                                                                                                                     aTAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATc                            <  1:1266337/150‑1 (MQ=255)
                                                                                                                        tcgaTGGCGTTGAATATTTTTTCAGCGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAgg                         >  2:914102/4‑150 (MQ=37)
                                                                                                                        aaaaTGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAgg                         >  2:594363/1‑150 (MQ=255)
                                                                                                                         cgaTGGCGTTGAATATTTTTTCAGCGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCATATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGc                        >  1:54904/3‑150 (MQ=37)
                                                                                                                          aaTGTCGCTGAAAAAATATTCAACTCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCa                       <  2:822317/150‑1 (MQ=255)
                                                                                                                           aaggCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCaa                      <  2:555370/148‑1 (MQ=255)
                                                                                                                            tGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAg                     <  2:1274427/150‑1 (MQ=255)
                                                                                                                              gcgcTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGAc                   >  1:1185899/1‑150 (MQ=255)
                                                                                                                               cgcTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCTTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATATGACGCGCCAGCGTCGCATCAGGCAAGAcg                  <  1:1004993/150‑2 (MQ=255)
                                                                                                                               cgcTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGAcc                  >  1:173679/1‑150 (MQ=255)
                                                                                                                                     aaaaaTATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTa            >  1:1075642/1‑150 (MQ=255)
                                                                                                                                     aaaaaTATTCAACGCCATCGACTTTTTATGCCAATGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGTCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTa            >  1:775700/1‑150 (MQ=255)
                                                                                                                                         atatTCACCGCCATCGACTTTTTATGCCTTTGCGGCAGCGGGCAATGCGGGTCGCATCCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTc        <  1:1019586/150‑1 (MQ=38)
                                                                                                                                         atatTCAACGCCATCGACTTATTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTc        <  1:510948/150‑1 (MQ=255)
                                                                                                                                          tatTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCg       <  1:561630/150‑1 (MQ=255)
                                                                                                                                            ttCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGCCCGTATTAATTCGGc     >  1:784770/1‑150 (MQ=255)
                                                                                                                                              cAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCAGCAGGCAAGACCGTATTAATTCGGCGt   >  2:873045/1‑150 (MQ=255)
                                                                                                                                               aaCGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGta  <  1:1215534/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
TGGCGTAATCAATCCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAAGACCGTATTAATTCGGCGTA  >  NC_000913/2430742‑2431034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: