Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,434,094 G→A 6.6% R216R (CGC→CGT dedA ← DedA family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,434,0940GA 6.6% 226.0 / 3.3 76R216R (CGC→CGTdedADedA family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (28/42);  new base A (2/3);  total (30/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.85e-01

ACCTGGTTCGATGCCAGTTTTATCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTAAAAAGCAGTACC  >  NC_000913/2433946‑2434241
                                                                                                                                                    |                                                                                                                                                   
aCCTGGTTCGATGCCAGTTTTATCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGgcgc                                                                                                                                                    >  2:451054/1‑150 (MQ=255)
aCCTGGTTCGATGCCAGTTTTATCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGgcgc                                                                                                                                                    >  2:455767/1‑150 (MQ=255)
    ggTTCGATGCCAGTTTTATCTTAGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGGCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGc                                                                                                                                                <  1:753430/150‑1 (MQ=255)
     gTTCGATGCCAGTTTTATCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTAATTTTTATTTTGCGGCGCTTGcc                                                                                                                                               >  1:698335/1‑150 (MQ=255)
       tCGATGCCAGTTTTATCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGc                                                                                                                                             <  1:409878/150‑1 (MQ=255)
       tCGATGCCAGTTTTATCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGc                                                                                                                                             <  1:421359/150‑1 (MQ=255)
           tGCCAGTTTTATCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAgcg                                                                                                                                         <  2:468202/150‑1 (MQ=255)
           agCCAGTTTTATCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGGCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAgcg                                                                                                                                         <  2:176541/149‑1 (MQ=255)
            tccAGTTTTATCTTTGGGGACTCATAATGACATTTTTCCCCCCAACAGACCAAGAATGTTTCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTATTTATTTTGCGGCACGCGCCGCaaaat                                                                                                                                        <  1:106882/149‑5 (MQ=255)
            gCCAGTTTTATCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAgcgc                                                                                                                                        >  2:1060088/1‑150 (MQ=255)
            gCCAGTTTTATCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCACGCGCCGCaaaat                                                                                                                                        <  1:1057046/150‑5 (MQ=255)
            gCCAGTTTTATCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCACGCGCCGCaaaaa                                                                                                                                        <  2:753080/150‑5 (MQ=255)
               aGTTTTATCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGtt                                                                                                                                     <  2:712655/150‑1 (MQ=255)
                 ttttATCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTtg                                                                                                                                   <  2:952144/150‑1 (MQ=255)
                 ttttATCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGGCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTtg                                                                                                                                   <  1:1236065/150‑1 (MQ=255)
                   ttATCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGACCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTtgtg                                                                                                                                 >  1:555834/1‑150 (MQ=255)
                     aTCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATTAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGAc                                                                                                                               <  2:749770/150‑1 (MQ=255)
                       cTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACgg                                                                                                                             >  1:115926/1‑150 (MQ=255)
                         ttGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGat                                                                                                                           <  2:1060062/150‑1 (MQ=255)
                           ggggACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACCGCCCGCAACTTTGGATAATAAAGTGGTCGAACCGCGGAGTTACGTTTTATTTTGCGGCGCGTGCCGCAGCGCGGTTGTGACGGagtt                                                                                                                         >  2:1063601/1‑147 (MQ=255)
                               aCGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTATAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTc                                                                                                                     <  2:472223/150‑1 (MQ=255)
                                  cATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGAt                                                                                                                  >  2:1066953/1‑150 (MQ=255)
                                   attaTGCCATATTTGCACCCAACAGACCATGAATGTTGCACATTAAATCATAACAGCCCGAAACTTTGGATAAAAAAGTGGGCGAACCGCGGAGTTACTTTTTTTTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATg                                                                                                                 <  2:310770/147‑1 (MQ=255)
                                         ccATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGccc                                                                                                           <  1:148552/150‑1 (MQ=255)
                                         ccATTTTTGACCCCAACAGACCATGACTGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCTCGTTTGTGACGGATTATTCCGATGACGccc                                                                                                           <  1:779173/150‑1 (MQ=255)
                                            tttttGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGc                                                                                                        <  1:1109085/150‑1 (MQ=255)
                                                tGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCaaaa                                                                                                    <  2:1273461/150‑1 (MQ=255)
                                                  cccccAACAGCCCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTTCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCAGTCAAAATa                                                                                                  >  2:152860/1‑150 (MQ=255)
                                                   ccccAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAg                                                                                                 >  1:359160/1‑150 (MQ=255)
                                                   ccccAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAg                                                                                                 >  2:699058/1‑150 (MQ=255)
                                                     ccAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGATTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGaa                                                                                               <  2:1165468/150‑1 (MQ=255)
                                                        aCAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAACAAAGTGGTCGAACCGCGGAGATACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAAcc                                                                                            <  1:25467/150‑1 (MQ=255)
                                                          aGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACcac                                                                                          <  2:970999/150‑1 (MQ=255)
                                                            aCCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACaa                                                                                        >  1:115959/1‑150 (MQ=255)
                                                             ccATGAATGTTGCACATTAAAACCTAACAGCCCGAAACTTTGGATAAAAAAGGGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACaat                                                                                       <  1:635633/150‑1 (MQ=255)
                                                                  aaTGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATcc                                                                                  <  2:581040/150‑1 (MQ=255)
                                                                     gTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCcgc                                                                               >  2:1271112/1‑149 (MQ=255)
                                                                      ttGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCcgac                                                                              <  1:973719/150‑1 (MQ=255)
                                                                       tGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCcgacg                                                                             <  2:581258/150‑1 (MQ=255)
                                                                         cacaTTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAATTAGAAACAACAATAATCCCGACTAt                                                                           >  2:1175819/1‑150 (MQ=255)
                                                                          acaTTAAAACATAACAGCCCGTAACTTTGGATAAAATAGTGGTCGATCCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGCCGCCCGGCAAAATAGAAGCCACAATAATCCcgacgagc                                                                          >  2:638025/1‑148 (MQ=255)
                                                                           caTTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATca                                                                         >  1:1114898/1‑150 (MQ=255)
                                                                             ttAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCTTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATcagc                                                                       <  2:569931/150‑1 (MQ=255)
                                                                               aaaaCATAAGAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATcagcag                                                                     <  1:200718/150‑1 (MQ=255)
                                                                                 aaCATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCt                                                                   <  1:545193/150‑1 (MQ=255)
                                                                                   cATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTATTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAGTAATCCCGACGATCAGCAGCTTa                                                                 >  1:172159/1‑150 (MQ=255)
                                                                                     tAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAg                                                               >  1:1142665/1‑150 (MQ=255)
                                                                                      aaCAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAgg                                                              <  2:1178746/150‑1 (MQ=255)
                                                                                       aCAGGCCGAAACTATGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGGGCCGCAGCGCGTGTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGt                                                             <  2:809451/150‑1 (MQ=255)
                                                                                       aCAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGt                                                             <  2:755675/150‑1 (MQ=255)
                                                                                            ccGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATcc                                                        <  2:1317371/150‑1 (MQ=255)
                                                                                                 aCTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAAc                                                   >  2:362676/1‑150 (MQ=255)
                                                                                                       gATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAACCCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCgg                                             >  1:280965/1‑150 (MQ=255)
                                                                                                       gATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCgg                                             <  1:1060088/150‑1 (MQ=255)
                                                                                                       gATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCgg                                             <  2:1235649/150‑1 (MQ=255)
                                                                                                       gATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCgg                                             <  1:1285942/150‑1 (MQ=255)
                                                                                                       gATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCgg                                             <  1:648073/150‑1 (MQ=255)
                                                                                                        aTAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCggg                                            <  1:884366/150‑1 (MQ=255)
                                                                                                         tAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGATCCATCGGGa                                           >  2:150342/1‑150 (MQ=255)
                                                                                                               aGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTAc                                     <  2:428741/150‑1 (MQ=255)
                                                                                                                  ggTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCga                                  >  2:670948/1‑150 (MQ=255)
                                                                                                                   gTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCgaa                                 >  1:1195672/1‑150 (MQ=255)
                                                                                                                     cGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCgaaga                               >  1:804568/1‑150 (MQ=255)
                                                                                                                         ccGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATa                           >  2:741879/1‑150 (MQ=255)
                                                                                                                         ccGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATa                           >  1:1270172/1‑150 (MQ=255)
                                                                                                                         ccGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACAACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGCATTGTACCGAAGaaaaa                           >  2:238542/1‑148 (MQ=255)
                                                                                                                         ccGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCATTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATa                           >  2:1147816/1‑150 (MQ=255)
                                                                                                                           gcgGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCTGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGc                         <  1:670948/150‑1 (MQ=255)
                                                                                                                            cgGAGTTACTTTTTATTTTTCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGCACCCTCGGGATTGTACCGAAGAAATatac                        >  1:902952/1‑147 (MQ=255)
                                                                                                                            cgGAGTTACTTTTTATTTTGCGTCGTGTGCCGCAGCGCGTTTTTGACGGATTTTTTCGATGACGCCCGGCAAAATAGAAACAACAATAATCACGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGTATTGTACCGAAGAAATAGcc                        <  2:855426/150‑1 (MQ=255)
                                                                                                                              gAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTg                      <  2:294153/150‑1 (MQ=255)
                                                                                                                               aGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGc                     >  2:1071517/1‑150 (MQ=255)
                                                                                                                                   aatttttATTTTGCGGCTCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCTGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAg                 <  2:797513/148‑1 (MQ=255)
                                                                                                                                         tATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAACCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGTAGAAATAGCCTGCGTAGGTaaaa           <  1:421011/150‑1 (MQ=255)
                                                                                                                                           ttttGCGGCGCGTGCCGCAGGGCGTTTGTGACGTATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTAAAAAg         <  1:1085608/150‑1 (MQ=255)
                                                                                                                                           cgctGCGGCACGCGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCTAAATAGAACCCACAATAATCCCGACGATCAGCAGCGTAAGGGGATCCTGCACCATGGGGAGTGTACCGAAGACAGAGCCTGCGTAGGTACAAAg         >  1:98928/4‑150 (MQ=255)
                                                                                                                                           cgctGCGGCACGCGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTAAAAAg         >  2:358823/4‑150 (MQ=255)
                                                                                                                                            tttGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTAAAAAGc        <  2:115926/150‑1 (MQ=255)
                                                                                                                                               gcggcgGGTGCCGCCGCGGGTTTGTGACGGAATATTACGATGACGCCCGGCAAAATAGAAACCACAATAAGCGCGTCGATCAGCAGCATATGCTTATCATGCACAAGCGGGATTGTACCGAAGAAGTAGCCTGCGTAGGTAAAAAGCAGt     >  1:345772/1‑150 (MQ=255)
                                                                                                                                               gcggcgCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTAAAAAGCAGt     >  2:1292152/1‑150 (MQ=255)
                                                                                                                                                cggcgCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTAAAAAGCAGTa    <  1:699058/150‑1 (MQ=255)
                                                                                                                                                  gcgcGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGCCGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTAAAAAGCAGTAcc  >  2:1050622/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ACCTGGTTCGATGCCAGTTTTATCTTTGGGGACGCATAATGCCATTTTTGCCCCCAACAGACCATGAATGTTGCACATTAAAACATAACAGCCCGAAACTTTGGATAAAAAAGTGGTCGAACCGCGGAGTTACTTTTTATTTTGCGGCGCGTGCCGCAGCGCGTTTGTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTAAAAAGCAGTACC  >  NC_000913/2433946‑2434241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: