| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,497,025 | C→A | 9.1% | intergenic (+33/+32) | ypdK → / ← alaC | inner membrane protein/glutamate‑pyruvate aminotransferase; glutamic‑pyruvic transaminase (GPT); alanine transaminase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,497,025 | 0 | C | A | 9.1% | 137.7 / 6.9 | 55 | intergenic (+33/+32) | ypdK/alaC | inner membrane protein/glutamate‑pyruvate aminotransferase; glutamic‑pyruvic transaminase (GPT); alanine transaminase |
| Reads supporting (aligned to +/- strand): ref base C (27/23); new base A (2/3); total (29/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.34e-01 | |||||||||||
GAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGC > NC_000913/2496878‑2497173 | gAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAg > 2:766096/1‑150 (MQ=255) aGTCATTCAGGCGTATCAGTAATTACGCGGAGAGATGTATAGTGATATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGc < 2:152465/150‑1 (MQ=255) cATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTcc > 2:1267500/1‑150 (MQ=255) cATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTcc > 2:246158/1‑150 (MQ=255) cAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGtt < 2:799400/150‑1 (MQ=255) ggCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGTGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCTGATGGCTCCTGtttt < 1:1186796/150‑2 (MQ=255) ggCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAGACAGGAGCCATCAGGCTCCTGTTTg < 2:720555/150‑1 (MQ=255) ggCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCTGATGGCTCCTGtttt < 1:175906/150‑2 (MQ=255) cggaaCAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGGCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCGCCTGTTTGCa < 1:1076630/147‑1 (MQ=255) tAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCAtt < 2:866168/150‑1 (MQ=255) aaCAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCATTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCTGATGGCTCCTGtttttcatt < 1:1059572/150‑7 (MQ=255) ctAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTAATGGTCATCGTTTGTGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTga < 1:1306351/149‑1 (MQ=255) gagaTGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGACATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTAt > 2:1236902/1‑150 (MQ=255) tGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCcg > 1:921472/1‑150 (MQ=255) tGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCcg > 1:544784/1‑150 (MQ=255) tAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCcgcg > 2:957854/1‑150 (MQ=255) tAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCACTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCcgcg > 1:732152/1‑150 (MQ=255) gAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCg > 1:1267461/1‑150 (MQ=255) aaaTATTTCTTTATGGGCATTTGTTTTATGGACATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGAAATGAAAAACTGGAGCCATCAGGCTCCTGTTTACATTTCTGACGACGGTGTTTATTCCGCGTTTTCGt < 2:634076/150‑1 (MQ=255) atTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAat > 2:982992/1‑150 (MQ=255) tttCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAata > 2:317116/1‑150 (MQ=255) tCTGTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCACTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAatatt > 1:20686/1‑149 (MQ=255) tttATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGaa > 1:511187/1‑144 (MQ=255) tttATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGaa < 2:511187/144‑1 (MQ=255) ttGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTtgc < 2:692597/149‑1 (MQ=255) gttttATGGTCATCTTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCggg < 2:927796/149‑1 (MQ=255) gTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAAc < 1:1042381/150‑1 (MQ=255) cATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCg > 2:424055/1‑150 (MQ=255) gTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCgg > 1:1299294/1‑150 (MQ=255) gTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTATCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCgg > 1:327024/1‑150 (MQ=255) cgCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACAGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGGTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACgg > 1:652352/2‑150 (MQ=255) ccGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTACACAGTCGGCACGGaa > 1:872737/1‑150 (MQ=255) ccTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTAGGCACGGAACATCGCTTTAATc > 1:246166/1‑150 (MQ=255) cTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATcc < 1:50446/150‑1 (MQ=255) cTGATGATCTAAAAGCAAACCGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATcc < 1:632318/150‑1 (MQ=255) tCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTACTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGAt > 2:298070/1‑150 (MQ=255) cTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATg < 1:694609/150‑1 (MQ=255) aaaaGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGc > 1:437635/1‑150 (MQ=255) aaaGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGGAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGcc < 1:618792/150‑1 (MQ=255) aaGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCt < 2:1267461/150‑1 (MQ=255) aaaCTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACg > 1:640826/1‑150 (MQ=255) cAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGt < 1:1206766/150‑1 (MQ=255) cAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGt < 1:1267500/150‑1 (MQ=255) aaaTGAAAAACAGGAGACATCATGCTCCTGTTTGCATTTCTGACGAAGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGt < 1:246158/149‑1 (MQ=255) aaaaaCAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCgg < 2:544784/150‑1 (MQ=255) aaaaaCAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGAGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCGCTCTGAGGGCCTGACGAATACGGTCGCgg > 2:5423/1‑150 (MQ=255) caaaCAGGAGCCTGATGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTATAATCCCTCTCATGGCCTGACGAATACGGTCGCGGt > 2:344100/2‑150 (MQ=255) aaaCAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAAGATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGtt < 1:138648/150‑1 (MQ=255) aaaCAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGAGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTGTAATGCCTCTGATGGCCTGACGAGTTCGGTCGCGGtt > 2:1065633/1‑150 (MQ=255) aCAGGAGCCTGATGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCAGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGtttt > 2:754165/1‑150 (MQ=255) aCAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGtttt > 1:1242319/1‑150 (MQ=255) gCCATCAGGCTCCTGTTTGCATTTCTGCCGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTACACCGTCGGCACGGAACATCGCTTTAAGCCCTCTGTTGGCCTGACGAATACGGTCGCGGTTTTCAATCa > 2:176352/1‑150 (MQ=255) ccATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAg > 1:446121/1‑150 (MQ=255) tCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGc < 2:797205/150‑1 (MQ=255) tCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGc < 1:688706/150‑1 (MQ=255) | GAAGTCATTCAGGCGTAACAGTAATTACGCGGAGAGATGTAAAGTGAAATATTTCTTTATGGGCATTTCTTTTATGGTCATCGTTTGGGCCGGTACTTTTGCCCTGATGATCTAAAAGCAAACTGTGCAATGAAAAACAGGAGCCATCAGGCTCCTGTTTGCATTTCTGACGACGGTGTTTATTCCGCGTTTTCGTGAATATGTTTGCTGCTGGCGGGTAGTAAACCGTCGGCACGGAACATCGCTTTAATCCCTCTGATGGCCTGACGAATACGGTCGCGGTTTTCAATCAGTGC > NC_000913/2496878‑2497173 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |