Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,560,321 C→T 5.2% A22V (GCT→GTT)  yffL → CPZ‑55 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,560,3210CT 5.2% 285.3 / 4.3 97A22V (GCT→GTT) yffLCPZ‑55 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (38/52);  new base T (3/2);  total (43/54)
Fisher's exact test for biased strand distribution p-value = 6.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.49e-01

Only 100 of 101 total aligned reads displayed.

GATTCGTCCGGCTTTTCTTTTTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAG  >  NC_000913/2560172‑2560470
                                                                                                                                                     |                                                                                                                                                     
gATTCGTCCGGCTTTTCTTTTTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAAtgc                                                                                                                                                       <  1:508685/150‑1 (MQ=255)
   tCGTCCGGCTTTTCTTTTTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAAtgctgc                                                                                                                                                    <  1:1242023/150‑1 (MQ=255)
      tCCGGCTTTTCTTTTTCGCTAATTATACCAATATTCCAATTAAGTGGAATAAAATAAACAATATCAAGAACTAACAACCAAGTTTGCAACTAAATATCCACAGTTCGAAAATAGGATCAACACAAAAAAAAGCCCCCGGAATGCTGCAAc                                                                                                                                                 >  2:618066/1‑150 (MQ=255)
        cGGCTTTTCTTTTTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGTTGCAACAt                                                                                                                                               <  1:911272/150‑1 (MQ=255)
            ttttcttatTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATTTTTGCAACGAAAGATCCAGAGTTCGAAAATAGAATCAACACAAATAAAAGCCCCCGGAATGATGCAACATGCCg                                                                                                                                           >  1:132534/1‑150 (MQ=255)
             tttcttttTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCgg                                                                                                                                          <  2:490013/150‑1 (MQ=255)
             tttcttttTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCgg                                                                                                                                          <  2:1039762/150‑1 (MQ=255)
              ttcttttTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCggg                                                                                                                                         >  1:651128/1‑150 (MQ=255)
              ttcttttTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCggg                                                                                                                                         >  1:318863/1‑150 (MQ=255)
                cttttTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGCATGTTGCAGCATTCCggggg                                                                                                                                       <  2:583827/150‑1 (MQ=255)
                cttttTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCggggg                                                                                                                                       <  2:1301359/150‑1 (MQ=255)
                cttttTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCggggg                                                                                                                                       <  2:1083791/150‑1 (MQ=255)
                cttttTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCggggg                                                                                                                                       <  1:754922/150‑1 (MQ=255)
                 tttttCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGc                                                                                                                                      >  1:329615/1‑150 (MQ=255)
                 tttttCGCTAATAATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAATAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGc                                                                                                                                      <  2:1140589/150‑1 (MQ=255)
                   tttCGCTATTTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCTTGTTTGCAACGAAAGATCCAGAGTGCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCCGCAACATGCCGGGGGCgg                                                                                                                                    <  2:214641/150‑1 (MQ=255)
                    ttCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTTCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGt                                                                                                                                   <  2:734641/150‑1 (MQ=255)
                      cGCTAATTATTCCATTATTCCAATTAAGTGGATTAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTAt                                                                                                                                 >  1:522531/1‑150 (MQ=255)
                          aaTTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATgaga                                                                                                                             <  2:1294838/150‑1 (MQ=255)
                               ttccattattccaATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACaa                                                                                                                        >  1:1114677/1‑150 (MQ=255)
                                 ccattattccaATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGc                                                                                                                      <  1:118297/150‑1 (MQ=255)
                                  cattattccaATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCt                                                                                                                     <  1:131822/150‑1 (MQ=255)
                                  cattattcaaATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCt                                                                                                                     <  2:1008687/150‑1 (MQ=255)
                                       ttccaATTAAGTGTAATAAATTATTCAATATCAAGAACTTAAATCAATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAgg                                                                                                                <  1:794120/150‑1 (MQ=255)
                                       ttccaATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAgg                                                                                                                <  1:887984/150‑1 (MQ=255)
                                       ttccaATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAgg                                                                                                                >  2:399080/1‑150 (MQ=255)
                                       ttccaATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAAACCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAgg                                                                                                                <  2:878326/150‑1 (MQ=255)
                                          caATTAAGTGGAATAAATTATTCAATATCTAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGATGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGgaga                                                                                                             >  1:1070983/1‑150 (MQ=255)
                                           aaTTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGaa                                                                                                            <  2:707337/150‑1 (MQ=255)
                                               aaGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTtc                                                                                                        >  2:1193096/1‑150 (MQ=255)
                                                 gTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTtctc                                                                                                      >  2:696393/1‑150 (MQ=255)
                                                   ggAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCAt                                                                                                    >  2:1036015/1‑150 (MQ=255)
                                                       tAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGTATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAGTTTCGCATGTCt                                                                                                >  1:788044/1‑150 (MQ=255)
                                                       tAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGATACAAGCTAAAGGAGAATTTCTCATGTcg                                                                                                >  2:406455/1‑149 (MQ=255)
                                                        aaaTTATTCAATATCAAGAACTTAAATCCATGTGTGCAACGAAAGATCCATAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTg                                                                                               <  2:1276268/150‑1 (MQ=255)
                                                        aaaTTATTCAATATCAAGAACTAAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTg                                                                                               <  2:931307/150‑1 (MQ=255)
                                                          attattCTATATAAAGAACTTAAATCCATGTTTGCAACGAAAGATCCTGAGTTCGAATATAGGATCAACACATATAAATGCCTGCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGat                                                                                             <  1:191106/150‑1 (MQ=255)
                                                              ttCAATATCAAGAACTTAACTCCATGATTGCAACTAAAGATACTGAGTACGAAAATCGGATCAACACAAATAAAATCCCCCGGAATGCTGCTACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGATAATTTCTCATGTCTGTTATGc                                                                                         <  1:46369/150‑1 (MQ=255)
                                                               tCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCt                                                                                        <  2:382843/150‑1 (MQ=255)
                                                                 aaTATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAg                                                                                      <  1:481257/150‑1 (MQ=255)
                                                                  atatCAATAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATATGATCAACACAAATAAAATCCCCCGTAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGc                                                                                     <  1:92102/150‑1 (MQ=255)
                                                                  atatCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGc                                                                                     <  2:172213/150‑1 (MQ=255)
                                                                    atCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAg                                                                                   <  1:1222346/150‑1 (MQ=255)
                                                                    atCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAg                                                                                   >  2:132486/1‑150 (MQ=255)
                                                                     tCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCACCACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGt                                                                                  >  1:951378/1‑150 (MQ=255)
                                                                     tCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGata                                                                                            <  1:997555/140‑1 (MQ=255)
                                                                      cAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGCTCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCTGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTa                                                                                 <  1:277317/150‑1 (MQ=255)
                                                                      cAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTa                                                                                 >  2:170650/1‑150 (MQ=255)
                                                                       aaGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAg                                                                                <  1:886091/150‑1 (MQ=255)
                                                                          aaCTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCAGATATGCTAGCAGTAGAAc                                                                             <  1:630993/150‑1 (MQ=255)
                                                                           aCTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACa                                                                            >  2:101042/1‑150 (MQ=255)
                                                                              tAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGa                                                                         >  1:1260301/1‑150 (MQ=255)
                                                                               aaaTCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGaa                                                                        <  1:774582/150‑1 (MQ=255)
                                                                                aaTCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGaaa                                                                       <  1:779407/150‑1 (MQ=255)
                                                                                  tCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGaaaca                                                                     <  1:1155565/150‑1 (MQ=255)
                                                                                  tCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGaaaca                                                                     <  2:1230509/150‑1 (MQ=255)
                                                                                          tGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAatga                                                             <  1:928233/150‑1 (MQ=255)
                                                                                          tGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAatga                                                             >  1:224011/1‑150 (MQ=255)
                                                                                          tGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAatga                                                             >  2:1156912/1‑150 (MQ=255)
                                                                                            cAACGAAAGATCCATAGATCGAAATTAGGATAAACAAAAATAAAAGCCGCCGGTATGCTGCAACATGACGGGGGCGGTATGATAAACAACCTAAAGGATAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAatgatg                                                           <  1:118288/150‑1 (MQ=255)
                                                                                            cAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACTAGAAACAAACAattatg                                                           >  2:145493/1‑150 (MQ=255)
                                                                                              aCGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGtt                                                         >  2:995778/1‑150 (MQ=255)
                                                                                              aCGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGtt                                                         >  1:156533/1‑150 (MQ=255)
                                                                                              aCGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTAATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGtt                                                         >  2:668438/1‑150 (MQ=255)
                                                                                                  aaGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGta                                                     >  2:902244/1‑149 (MQ=255)
                                                                                                     aTCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACATTGATGTTCGTCAAt                                                  >  2:715467/1‑150 (MQ=255)
                                                                                                        cAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAAtttt                                               >  2:1266733/1‑150 (MQ=255)
                                                                                                         agagTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACACACAATGATGTTCGTCAAttttt                                              >  1:764944/1‑150 (MQ=255)
                                                                                                          tatttCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTa                                             <  1:934347/147‑1 (MQ=255)
                                                                                                          gagTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAATCTAAAGGAGAATTTCACATGTCAGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTa                                             <  1:619756/150‑1 (MQ=255)
                                                                                                          gagTTCGAAAATAGGATCAAAACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGTGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTa                                             <  1:926682/150‑1 (MQ=255)
                                                                                                            gTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGCCGGTATGAGAAACAAGCAAAAGGAGAAATTCACATGTCTGATATGCTAGCAGAAGAACAAGAAACAAACAATGATGTTCATCAATATTTaaa                                           >  1:554815/1‑150 (MQ=255)
                                                                                                             ttCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAAc                                          <  2:329615/150‑1 (MQ=255)
                                                                                                              tCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACa                                         <  1:686309/150‑1 (MQ=255)
                                                                                                                       ggATCAACACAAATAAAAGCCCCCGGAATTCTGCAACATGCCGGGGGCCGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATAAGCTAGCAGTAGAACAAGATACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACg                                >  2:192480/1‑150 (MQ=255)
                                                                                                                       ggATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACg                                >  2:978056/1‑150 (MQ=255)
                                                                                                                        gATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGa                               <  2:942344/150‑1 (MQ=255)
                                                                                                                        gATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTAAAACAAAATTAACGa                               >  1:981990/1‑150 (MQ=255)
                                                                                                                          tctacacATATAAAATCACCCGGATTTCTGCAACATGCCGGTGTCGGTATGAGAAACAAGATAAAGGAGAATTTCTCATTTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAAAGAGt                             <  2:330467/147‑1 (MQ=255)
                                                                                                                           cAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGtt                            >  2:1053108/1‑150 (MQ=255)
                                                                                                                           cAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAACCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGtt                            >  2:523631/1‑150 (MQ=255)
                                                                                                                              cacaAATAAAAGCCCCCGGAAAGCTGCAACATGCCGCGGGCGGTATGAGAAACAAGATAAAGGATAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACg                         <  1:191629/150‑1 (MQ=255)
                                                                                                                                caAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGa                       >  2:69327/1‑150 (MQ=255)
                                                                                                                                  aaTAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAAt                     >  2:1159404/1‑150 (MQ=255)
                                                                                                                                  aaTAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAAt                     >  1:1111946/1‑150 (MQ=255)
                                                                                                                                    tAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCTTGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATaa                   <  2:69474/150‑1 (MQ=255)
                                                                                                                                      aaaGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAg                 <  1:487366/150‑1 (MQ=255)
                                                                                                                                        cgCCCCCGGCATGTTGCAGCATTCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCa               >  1:846556/2‑150 (MQ=255)
                                                                                                                                          ccccccGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCAcc             <  1:202033/150‑1 (MQ=255)
                                                                                                                                           ccccGGCATGTTGCAGCATTCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCAccc            >  1:597157/1‑150 (MQ=255)
                                                                                                                                           ccccGGCATGTTGCAGCATTCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCAGGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCAccc            >  1:857428/1‑150 (MQ=255)
                                                                                                                                           ccccGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCAccc            <  2:1194229/150‑1 (MQ=255)
                                                                                                                                            cccGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCa           <  2:951378/150‑1 (MQ=255)
                                                                                                                                            cccGGAATGCAGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCa           >  1:586346/1‑150 (MQ=255)
                                                                                                                                                 aaTGCTGCAACATGCCGGGGGCGGTATGATAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAAc      <  2:318863/150‑1 (MQ=255)
                                                                                                                                                 aaTGCGGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTTATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAAc      <  2:83366/150‑1 (MQ=255)
                                                                                                                                                  aTGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACg     <  2:588420/150‑1 (MQ=255)
                                                                                                                                                  aTGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACg     <  2:1070983/150‑1 (MQ=255)
                                                                                                                                                     ctgcAAGATGCCGGGGGAGGTATGAGAAACAAGCTAAAGGAGAATTTCTCTTGTCTGATATGGTTGCAGTTGATCAAGAAAAAAACAATGATGTTCGTCAATTTTTAAACTAACTTAACGAGTTACGTCTTAAAGGACCCAATAACGAag  <  2:710695/150‑1 (MQ=255)
                                                                                                                                                     ctgcAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAag  >  2:472859/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GATTCGTCCGGCTTTTCTTTTTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAG  >  NC_000913/2560172‑2560470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: