Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,587,007 C→T 5.2% T426M (ACG→ATG) ‡ narQ → sensory histidine kinase in two‑component regulatory system with NarP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,587,0070CT 5.2% 295.0 / 3.2 97T426M (ACG→ATG) ‡narQsensory histidine kinase in two‑component regulatory system with NarP
Reads supporting (aligned to +/- strand):  ref base C (48/43);  new base T (2/3);  total (51/46)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.51e-01

CTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGC  >  NC_000913/2586863‑2587155
                                                                                                                                                |                                                                                                                                                    
cttacttaCGTATCCAGTTGACGGTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTgc                                                                                                                                                 <  2:943999/150‑1 (MQ=255)
 ttacttacGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTgca                                                                                                                                                <  2:1283455/150‑1 (MQ=255)
  tacttacGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTgcag                                                                                                                                               >  2:39223/1‑150 (MQ=255)
    cttacGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTgcagca                                                                                                                                             >  2:1188122/1‑150 (MQ=255)
    cttacGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTgcagca                                                                                                                                             <  2:786563/150‑1 (MQ=255)
      tacGTATCCCGTTGACGTTACTGACGCGTTCGATCCCGGACGATAACGCCACCGCACAACGTATCATGGCCGATTTTTCCCCGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAgg                                                                                                                                           <  2:1112744/150‑1 (MQ=255)
        cGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGCTGCAGCGTCAGGCg                                                                                                                                         <  2:420283/150‑1 (MQ=255)
        cGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCg                                                                                                                                         <  1:1160493/150‑1 (MQ=255)
        cGTATCCAGTTGACGTTACTCCAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCCTGGCCGATTATTCCCCGGCGGTGAATGATGCTTATCGGCAGTAACGCGAGCGGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCg                                                                                                                                         <  2:194194/150‑1 (MQ=255)
        agTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCg                                                                                                                                         <  2:803956/149‑1 (MQ=255)
         gTATCCAGTAGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGCTGCAGCGTCAGGCgg                                                                                                                                        <  1:742385/150‑1 (MQ=255)
            tCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGCTGCAGCGTCAGGCGGaaag                                                                                                                                    <  2:537979/150‑4 (MQ=255)
               aGTTGACGTTACTGCAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCGGACGCTGCAGCAGGCGGATCTcc                                                                                                                                  <  1:545969/150‑1 (MQ=255)
               aGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTcc                                                                                                                                  <  1:405109/150‑1 (MQ=255)
                  tGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAATGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACGTTCCGCCTGACGCTGCAGCCGGCGGATCTccccg                                                                                                                               >  1:618509/1‑149 (MQ=255)
                  tGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGCCTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCt                                                                                                                               >  1:1118754/1‑150 (MQ=255)
                   gACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTAACGCGAGCTGTTGACTGCTTTCCGCCTGCCGCTGCAGCACGCGGATCTCCCCTc                                                                                                                              >  2:528028/1‑150 (MQ=255)
                    aCGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTcc                                                                                                                             >  2:76910/1‑150 (MQ=255)
                        tACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAt                                                                                                                         <  2:969475/150‑1 (MQ=255)
                         aCTGATGCGTTCGATAGCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTACCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAtt                                                                                                                        <  1:17545/150‑1 (MQ=255)
                          cTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTg                                                                                                                       <  2:781151/150‑1 (MQ=255)
                           tgccgCGTCCGAAACCGGACGAAAACGCCACCGCACAAAGTCTCATGGCCTATTTTTCCCCGGCGCTGAATGATGCTAATCGGCAGTTACGCGAGCTGTTGTCTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGa                                                                                                                      <  2:73827/146‑1 (MQ=255)
                            gAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAg                                                                                                                     >  2:392786/1‑150 (MQ=255)
                            gAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAg                                                                                                                     <  1:1183534/150‑1 (MQ=255)
                            gAAGCGTTCGATACCGGAAGATAACGCAAACGCCCAAAGTATCATGGCCGATTTTTCCCAGGCTGTGAATGCTGATTATCGGCAGTGCCGAGCGCGGTTGACGACTTTCCGCCTGCCGCTGCAGCCGGCGGGGCTCCCCTCCGCATTGAg                                                                                                                     >  2:196757/1‑150 (MQ=255)
                              aGCGTTCGATACCGGAAGATAACGCAACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAggg                                                                                                                   <  1:234370/150‑1 (MQ=255)
                               gCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGa                                                                                                                  >  2:1036953/1‑150 (MQ=255)
                                 gTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGaaa                                                                                                                >  2:527796/1‑150 (MQ=255)
                                   tCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATg                                                                                                              >  1:929109/1‑150 (MQ=255)
                                    cGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATgg                                                                                                             >  2:919781/1‑149 (MQ=255)
                                    cGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGc                                                                                                             <  1:665000/150‑1 (MQ=255)
                                      aTACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTg                                                                                                           >  1:774002/1‑150 (MQ=255)
                                        aCCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGa                                                                                                         >  2:420027/1‑150 (MQ=255)
                                        aCCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGa                                                                                                         >  2:957784/1‑150 (MQ=255)
                                        aCCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGa                                                                                                         >  1:19028/1‑150 (MQ=255)
                                         ccGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGCCGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGAt                                                                                                        >  2:1232197/1‑150 (MQ=255)
                                              aGATAACGCCACCGCACAAAGTATCATGGCCGATATTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGtt                                                                                                   <  2:531109/150‑1 (MQ=255)
                                                aTAACGCCACCGCACACAGTATCATGGCCGTATTATCCCCGGCGGTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTAc                                                                                                 <  2:94851/150‑1 (MQ=255)
                                                  aaCGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACaa                                                                                               <  2:48197/150‑1 (MQ=255)
                                                  aaCGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTAAGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACaa                                                                                               >  1:131253/1‑150 (MQ=255)
                                                           gCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAAcc                                                                                      >  1:408672/1‑150 (MQ=255)
                                                           gCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAAcc                                                                                      <  1:324147/150‑1 (MQ=255)
                                                             acaAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAg                                                                                    <  1:691066/150‑1 (MQ=255)
                                                             acaAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAg                                                                                    >  1:1174540/1‑150 (MQ=255)
                                                                 aGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTAGAAAATCAAACCAGCGcc                                                                                <  1:130898/150‑1 (MQ=255)
                                                                   tATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCaa                                                                              >  2:41446/1‑150 (MQ=255)
                                                                    aTCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCaaa                                                                             <  2:194731/150‑1 (MQ=255)
                                                                    aTCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCaaa                                                                             >  1:936372/1‑150 (MQ=255)
                                                                        tGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTgc                                                                         >  2:291043/1‑149 (MQ=255)
                                                                         ggCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGAc                                                                        >  1:931465/1‑150 (MQ=255)
                                                                           ccGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGAccc                                                                      <  1:456158/150‑1 (MQ=255)
                                                                                  ttCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCACCAAACTGACCCTCGACTg                                                               <  1:1232197/150‑1 (MQ=255)
                                                                                   tCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTTGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGc                                                              <  2:1090534/150‑1 (MQ=255)
                                                                                   tCCCAGGCGGTGAATGATGCGTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGc                                                              <  1:232537/150‑1 (MQ=255)
                                                                                        ggCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGCTCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCt                                                         >  1:202620/1‑150 (MQ=255)
                                                                                        ggCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCt                                                         >  1:22348/1‑150 (MQ=255)
                                                                                         gCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTg                                                        <  1:946274/150‑1 (MQ=255)
                                                                                          cGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAACCCAGCGCCAAACTGACCATCGACTGCCGTCTGc                                                       >  2:64899/1‑150 (MQ=255)
                                                                                          cGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGc                                                       <  1:1039111/150‑1 (MQ=255)
                                                                                           gTTGAATGATTCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGAAGCTGCAGCAGGCGGATCTCCCCTCCGCAGTGAGGGAAATGATGGATACGTTACAAAATCAAACCAGCGCCAACCTGACCCTCTCCTGCCGTCTGcc                                                      >  2:621984/1‑150 (MQ=255)
                                                                                           gTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGCCTGCCGTCTGcc                                                      >  1:887274/1‑150 (MQ=255)
                                                                                             tGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCaa                                                    <  1:385972/150‑1 (MQ=255)
                                                                                              gAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACACTCGACTGCCGTCTGCCAAc                                                   >  1:650744/1‑150 (MQ=255)
                                                                                              gAATGATGCTTATCGGCAGTTACGCGAGCTGGTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAAc                                                   <  1:500279/150‑1 (MQ=255)
                                                                                               aaTGTTGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAAcc                                                  <  2:420944/150‑1 (MQ=255)
                                                                                                  gatgCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTg                                               >  1:1031350/1‑150 (MQ=255)
                                                                                                    tgCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCACGACTGCCGTCTGCCAACCCTgcc                                             >  2:864212/1‑148 (MQ=255)
                                                                                                     gCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCa                                            >  2:537504/1‑150 (MQ=255)
                                                                                                        tATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTg                                         <  1:22792/150‑1 (MQ=255)
                                                                                                           cGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCAGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGAt                                      <  2:349/150‑1 (MQ=255)
                                                                                                            ggCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGCCCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATg                                     >  1:725259/1‑150 (MQ=255)
                                                                                                             gCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGCCCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATgc                                    >  2:829052/1‑150 (MQ=255)
                                                                                                             gCAGTTACGCGAGCTGGTGACTACTTTCCGCCTGACCCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATgc                                    <  2:863601/150‑1 (MQ=255)
                                                                                                                     gcgAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGc                            <  1:561776/150‑1 (MQ=255)
                                                                                                                     gcgAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGc                            >  2:724833/1‑150 (MQ=255)
                                                                                                                      cgAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCCGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTGACAAAATCAAACCAGCGCCACACTGACCCTCGCCAGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCa                           >  2:837992/1‑150 (MQ=255)
                                                                                                                        aGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAgg                         >  2:908202/1‑150 (MQ=255)
                                                                                                                          cTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGCCCCTCGCCTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTg                       >  2:872806/1‑150 (MQ=255)
                                                                                                                          cTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTg                       <  1:1188122/150‑1 (MQ=255)
                                                                                                                          cTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACACTCGACTGCAGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTg                       >  1:653905/1‑150 (MQ=255)
                                                                                                                              tGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCAtt                   >  2:141460/1‑150 (MQ=255)
                                                                                                                               gACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGGATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCCAACTGCCCCTCGACTGGCGTCTGCCAACCCTGGCTCTGGATGCGCAAATGCGGGTGCAttt                  >  1:1130782/1‑150 (MQ=255)
                                                                                                                                 ctactTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATttgt                <  2:440646/150‑1 (MQ=255)
                                                                                                                                 ctactTTCCGCCTGACGCGGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCACCTGCATttgc                <  1:109468/150‑2 (MQ=255)
                                                                                                                                  tactTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATttgtt               <  2:106472/150‑1 (MQ=255)
                                                                                                                                  tactTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATttgtt               <  1:504267/150‑1 (MQ=255)
                                                                                                                                    ctTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGc             >  2:174406/1‑150 (MQ=255)
                                                                                                                                     tttCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCa            >  2:1036563/1‑150 (MQ=255)
                                                                                                                                      atCCGCCTGCTGCAGCGTCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACAAGCGCCAAACTGACCCTCGACTGCCGTCTGCCCACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCaa           >  2:798853/2‑150 (MQ=255)
                                                                                                                                       tCCGCCTGCTGCAGCGTCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCaaa          >  2:322732/1‑150 (MQ=255)
                                                                                                                                        ccGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAat         >  1:673750/1‑150 (MQ=255)
                                                                                                                                         cGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCCGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAatt        >  2:1101660/1‑150 (MQ=255)
                                                                                                                                         cGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACACTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAatt        >  2:128859/1‑150 (MQ=255)
                                                                                                                                          gCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGGCCACCCTGGCACTGGTGGCGCTAATGGAGGTGCAGTTGTGGCattttt       >  2:1212636/1‑145 (MQ=255)
                                                                                                                                             tGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTc    >  2:1260724/1‑150 (MQ=255)
                                                                                                                                              gACGCGGCAGCAGGCGGATCTCCCCTCCGCTATGAGGGAAATGCTGGATACGTTACAAAATCAACCCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTcg   <  1:1146808/150‑1 (MQ=255)
                                                                                                                                               aCGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCGGCGCCAAACTGACCCTCAACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTcgc  <  2:1115406/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
CTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGC  >  NC_000913/2586863‑2587155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: