Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,587,013 A→G 5.3% Q428R (CAG→CGG) ‡ narQ → sensory histidine kinase in two‑component regulatory system with NarP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,587,0130AG 5.3% 280.6 / 5.4 94Q428R (CAG→CGG) ‡narQsensory histidine kinase in two‑component regulatory system with NarP
Reads supporting (aligned to +/- strand):  ref base A (48/41);  new base G (2/3);  total (50/44)
Fisher's exact test for biased strand distribution p-value = 6.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.92e-01

TTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGC  >  NC_000913/2586864‑2587160
                                                                                                                                                     |                                                                                                                                                   
ttacttacGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTgca                                                                                                                                                     <  2:1283455/150‑1 (MQ=255)
 tacttacGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTgcag                                                                                                                                                    >  2:39223/1‑150 (MQ=255)
   cttacGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTgcagca                                                                                                                                                  >  2:1188122/1‑150 (MQ=255)
   cttacGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTgcagca                                                                                                                                                  <  2:786563/150‑1 (MQ=255)
     tacGTATCCCGTTGACGTTACTGACGCGTTCGATCCCGGACGATAACGCCACCGCACAACGTATCATGGCCGATTTTTCCCCGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAgg                                                                                                                                                <  2:1112744/150‑1 (MQ=255)
       cGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGCTGCAGCGTCAGGCg                                                                                                                                              <  2:420283/150‑1 (MQ=255)
       cGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCg                                                                                                                                              <  1:1160493/150‑1 (MQ=255)
       cGTATCCAGTTGACGTTACTCCAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCCTGGCCGATTATTCCCCGGCGGTGAATGATGCTTATCGGCAGTAACGCGAGCGGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCg                                                                                                                                              <  2:194194/150‑1 (MQ=255)
       agTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCg                                                                                                                                              <  2:803956/149‑1 (MQ=255)
        gTATCCAGTAGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGCTGCAGCGTCAGGCgg                                                                                                                                             <  1:742385/150‑1 (MQ=255)
           tCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGCTGCAGCGTCAGGCGGaaag                                                                                                                                         <  2:537979/150‑4 (MQ=255)
              aGTTGACGTTACTGCAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCGGACGCTGCAGCAGGCGGATCTcc                                                                                                                                       <  1:545969/150‑1 (MQ=255)
              aGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTcc                                                                                                                                       <  1:405109/150‑1 (MQ=255)
                 tGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAATGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACGTTCCGCCTGACGCTGCAGCCGGCGGATCTccccg                                                                                                                                    >  1:618509/1‑149 (MQ=255)
                 tGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGCCTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCt                                                                                                                                    >  1:1118754/1‑150 (MQ=255)
                  gACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTAACGCGAGCTGTTGACTGCTTTCCGCCTGCCGCTGCAGCACGCGGATCTCCCCTc                                                                                                                                   >  2:528028/1‑150 (MQ=255)
                   aCGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTcc                                                                                                                                  >  2:76910/1‑150 (MQ=255)
                       tACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAt                                                                                                                              <  2:969475/150‑1 (MQ=255)
                        aCTGATGCGTTCGATAGCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTACCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAtt                                                                                                                             <  1:17545/150‑1 (MQ=255)
                         cTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTg                                                                                                                            <  2:781151/150‑1 (MQ=255)
                          tgccgCGTCCGAAACCGGACGAAAACGCCACCGCACAAAGTCTCATGGCCTATTTTTCCCCGGCGCTGAATGATGCTAATCGGCAGTTACGCGAGCTGTTGTCTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGa                                                                                                                           <  2:73827/146‑1 (MQ=255)
                           gAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAg                                                                                                                          >  2:392786/1‑150 (MQ=255)
                           gAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAg                                                                                                                          <  1:1183534/150‑1 (MQ=255)
                           gAAGCGTTCGATACCGGAAGATAACGCAAACGCCCAAAGTATCATGGCCGATTTTTCCCAGGCTGTGAATGCTGATTATCGGCAGTGCCGAGCGCGGTTGACGACTTTCCGCCTGCCGCTGCAGCCGGCGGGGCTCCCCTCCGCATTGAg                                                                                                                          >  2:196757/1‑150 (MQ=255)
                             aGCGTTCGATACCGGAAGATAACGCAACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAggg                                                                                                                        <  1:234370/150‑1 (MQ=255)
                              gCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGa                                                                                                                       >  2:1036953/1‑150 (MQ=255)
                                gTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGaaa                                                                                                                     >  2:527796/1‑150 (MQ=255)
                                  tCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATg                                                                                                                   >  1:929109/1‑150 (MQ=255)
                                   cGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATgg                                                                                                                  >  2:919781/1‑149 (MQ=255)
                                   cGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGc                                                                                                                  <  1:665000/150‑1 (MQ=255)
                                     aTACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTg                                                                                                                >  1:774002/1‑150 (MQ=255)
                                       aCCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGa                                                                                                              >  2:957784/1‑150 (MQ=255)
                                       aCCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGa                                                                                                              >  2:420027/1‑150 (MQ=255)
                                       aCCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGa                                                                                                              >  1:19028/1‑150 (MQ=255)
                                        ccGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGCCGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGAt                                                                                                             >  2:1232197/1‑150 (MQ=255)
                                             aGATAACGCCACCGCACAAAGTATCATGGCCGATATTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGtt                                                                                                        <  2:531109/150‑1 (MQ=255)
                                               aTAACGCCACCGCACACAGTATCATGGCCGTATTATCCCCGGCGGTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTAc                                                                                                      <  2:94851/150‑1 (MQ=255)
                                                 aaCGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACaa                                                                                                    <  2:48197/150‑1 (MQ=255)
                                                 aaCGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTAAGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACaa                                                                                                    >  1:131253/1‑150 (MQ=255)
                                                          gCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAAcc                                                                                           >  1:408672/1‑150 (MQ=255)
                                                          gCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAAcc                                                                                           <  1:324147/150‑1 (MQ=255)
                                                            acaAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAg                                                                                         <  1:691066/150‑1 (MQ=255)
                                                            acaAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAg                                                                                         >  1:1174540/1‑150 (MQ=255)
                                                                aGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTAGAAAATCAAACCAGCGcc                                                                                     <  1:130898/150‑1 (MQ=255)
                                                                  tATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCaa                                                                                   >  2:41446/1‑150 (MQ=255)
                                                                   aTCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCaaa                                                                                  <  2:194731/150‑1 (MQ=255)
                                                                   aTCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCaaa                                                                                  >  1:936372/1‑150 (MQ=255)
                                                                       tGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTgc                                                                              >  2:291043/1‑149 (MQ=255)
                                                                        ggCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGAc                                                                             >  1:931465/1‑150 (MQ=255)
                                                                          ccGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGAccc                                                                           <  1:456158/150‑1 (MQ=255)
                                                                                 ttCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCACCAAACTGACCCTCGACTg                                                                    <  1:1232197/150‑1 (MQ=255)
                                                                                  tCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTTGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGc                                                                   <  2:1090534/150‑1 (MQ=255)
                                                                                  tCCCAGGCGGTGAATGATGCGTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGc                                                                   <  1:232537/150‑1 (MQ=255)
                                                                                       ggCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGCTCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCt                                                              >  1:202620/1‑150 (MQ=255)
                                                                                       ggCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCt                                                              >  1:22348/1‑150 (MQ=255)
                                                                                        gCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTg                                                             <  1:946274/150‑1 (MQ=255)
                                                                                         cGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAACCCAGCGCCAAACTGACCATCGACTGCCGTCTGc                                                            >  2:64899/1‑150 (MQ=255)
                                                                                         cGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGc                                                            <  1:1039111/150‑1 (MQ=255)
                                                                                          gTTGAATGATTCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGAAGCTGCAGCAGGCGGATCTCCCCTCCGCAGTGAGGGAAATGATGGATACGTTACAAAATCAAACCAGCGCCAACCTGACCCTCTCCTGCCGTCTGcc                                                           >  2:621984/1‑150 (MQ=255)
                                                                                          gTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGCCTGCCGTCTGcc                                                           >  1:887274/1‑150 (MQ=255)
                                                                                            tGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCaa                                                         <  1:385972/150‑1 (MQ=255)
                                                                                             gAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACACTCGACTGCCGTCTGCCAAc                                                        >  1:650744/1‑150 (MQ=255)
                                                                                             gAATGATGCTTATCGGCAGTTACGCGAGCTGGTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAAc                                                        <  1:500279/150‑1 (MQ=255)
                                                                                              aaTGTTGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAAcc                                                       <  2:420944/150‑1 (MQ=255)
                                                                                                 gatgCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTg                                                    >  1:1031350/1‑150 (MQ=255)
                                                                                                   tgCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCACGACTGCCGTCTGCCAACCCTgcc                                                  >  2:864212/1‑148 (MQ=255)
                                                                                                    gCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCa                                                 >  2:537504/1‑150 (MQ=255)
                                                                                                       tATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTg                                              <  1:22792/150‑1 (MQ=255)
                                                                                                          cGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCAGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGAt                                           <  2:349/150‑1 (MQ=255)
                                                                                                           ggCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGCCCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATg                                          >  1:725259/1‑150 (MQ=255)
                                                                                                            gCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGCCCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATgc                                         >  2:829052/1‑150 (MQ=255)
                                                                                                            gCAGTTACGCGAGCTGGTGACTACTTTCCGCCTGACCCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATgc                                         <  2:863601/150‑1 (MQ=255)
                                                                                                                    gcgAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGc                                 >  2:724833/1‑150 (MQ=255)
                                                                                                                    gcgAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGc                                 <  1:561776/150‑1 (MQ=255)
                                                                                                                     cgAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCCGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTGACAAAATCAAACCAGCGCCACACTGACCCTCGCCAGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCa                                >  2:837992/1‑150 (MQ=255)
                                                                                                                       aGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAgg                              >  2:908202/1‑150 (MQ=255)
                                                                                                                         cTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGCCCCTCGCCTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTg                            >  2:872806/1‑150 (MQ=255)
                                                                                                                         cTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTg                            <  1:1188122/150‑1 (MQ=255)
                                                                                                                         cTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACACTCGACTGCAGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTg                            >  1:653905/1‑150 (MQ=255)
                                                                                                                             tGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCAtt                        >  2:141460/1‑150 (MQ=255)
                                                                                                                              gACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGGATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCCAACTGCCCCTCGACTGGCGTCTGCCAACCCTGGCTCTGGATGCGCAAATGCGGGTGCAttt                       >  1:1130782/1‑150 (MQ=255)
                                                                                                                                ctactTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATttgt                     <  2:440646/150‑1 (MQ=255)
                                                                                                                                ctactTTCCGCCTGACGCGGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCACCTGCATttgc                     <  1:109468/150‑2 (MQ=255)
                                                                                                                                 tactTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATttgtt                    <  1:504267/150‑1 (MQ=255)
                                                                                                                                 tactTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATttgtt                    <  2:106472/150‑1 (MQ=255)
                                                                                                                                   ctTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGc                  >  2:174406/1‑150 (MQ=255)
                                                                                                                                    tttCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCa                 >  2:1036563/1‑150 (MQ=255)
                                                                                                                                     atCCGCCTGCTGCAGCGTCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACAAGCGCCAAACTGACCCTCGACTGCCGTCTGCCCACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCaa                >  2:798853/2‑150 (MQ=255)
                                                                                                                                      tCCGCCTGCTGCAGCGTCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCaaa               >  2:322732/1‑150 (MQ=255)
                                                                                                                                       ccGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAat              >  1:673750/1‑150 (MQ=255)
                                                                                                                                        cGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCCGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAatt             >  2:1101660/1‑150 (MQ=255)
                                                                                                                                        cGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACACTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAatt             >  2:128859/1‑150 (MQ=255)
                                                                                                                                         gCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGGCCACCCTGGCACTGGTGGCGCTAATGGAGGTGCAGTTGTGGCattttt            >  2:1212636/1‑145 (MQ=255)
                                                                                                                                            tGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTc         >  2:1260724/1‑150 (MQ=255)
                                                                                                                                             gACGCGGCAGCAGGCGGATCTCCCCTCCGCTATGAGGGAAATGCTGGATACGTTACAAAATCAACCCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTcg        <  1:1146808/150‑1 (MQ=255)
                                                                                                                                              aCGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCGGCGCCAAACTGACCCTCAACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTcgc       <  2:1115406/150‑1 (MQ=255)
                                                                                                                                                  tGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAg   <  1:39223/150‑1 (MQ=255)
                                                                                                                                                  tGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAg   >  1:583176/1‑150 (MQ=255)
                                                                                                                                                   gcagcaGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGc  >  2:390105/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGC  >  NC_000913/2586864‑2587160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: