Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,587,014 G→T 5.3% Q428H (CAG→CAT) ‡ narQ → sensory histidine kinase in two‑component regulatory system with NarP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,587,0140GT 5.3% 275.3 / 3.2 94Q428H (CAG→CAT) ‡narQsensory histidine kinase in two‑component regulatory system with NarP
Reads supporting (aligned to +/- strand):  ref base G (48/41);  new base T (2/3);  total (50/44)
Fisher's exact test for biased strand distribution p-value = 6.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01

TACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGC  >  NC_000913/2586865‑2587160
                                                                                                                                                     |                                                                                                                                                  
tacttacGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTgcag                                                                                                                                                    >  2:39223/1‑150 (MQ=255)
  cttacGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTgcagca                                                                                                                                                  >  2:1188122/1‑150 (MQ=255)
  cttacGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTgcagca                                                                                                                                                  <  2:786563/150‑1 (MQ=255)
    tacGTATCCCGTTGACGTTACTGACGCGTTCGATCCCGGACGATAACGCCACCGCACAACGTATCATGGCCGATTTTTCCCCGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAgg                                                                                                                                                <  2:1112744/150‑1 (MQ=255)
      cGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGCTGCAGCGTCAGGCg                                                                                                                                              <  2:420283/150‑1 (MQ=255)
      cGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCg                                                                                                                                              <  1:1160493/150‑1 (MQ=255)
      cGTATCCAGTTGACGTTACTCCAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCCTGGCCGATTATTCCCCGGCGGTGAATGATGCTTATCGGCAGTAACGCGAGCGGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCg                                                                                                                                              <  2:194194/150‑1 (MQ=255)
      agTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCg                                                                                                                                              <  2:803956/149‑1 (MQ=255)
       gTATCCAGTAGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGCTGCAGCGTCAGGCgg                                                                                                                                             <  1:742385/150‑1 (MQ=255)
          tCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGCTGCAGCGTCAGGCGGaaag                                                                                                                                         <  2:537979/150‑4 (MQ=255)
             aGTTGACGTTACTGCAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCGGACGCTGCAGCAGGCGGATCTcc                                                                                                                                       <  1:545969/150‑1 (MQ=255)
             aGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTcc                                                                                                                                       <  1:405109/150‑1 (MQ=255)
                tGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAATGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACGTTCCGCCTGACGCTGCAGCCGGCGGATCTccccg                                                                                                                                    >  1:618509/1‑149 (MQ=255)
                tGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGCCTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCt                                                                                                                                    >  1:1118754/1‑150 (MQ=255)
                 gACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTAACGCGAGCTGTTGACTGCTTTCCGCCTGCCGCTGCAGCACGCGGATCTCCCCTc                                                                                                                                   >  2:528028/1‑150 (MQ=255)
                  aCGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTcc                                                                                                                                  >  2:76910/1‑150 (MQ=255)
                      tACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAt                                                                                                                              <  2:969475/150‑1 (MQ=255)
                       aCTGATGCGTTCGATAGCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTACCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAtt                                                                                                                             <  1:17545/150‑1 (MQ=255)
                        cTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTg                                                                                                                            <  2:781151/150‑1 (MQ=255)
                         tgccgCGTCCGAAACCGGACGAAAACGCCACCGCACAAAGTCTCATGGCCTATTTTTCCCCGGCGCTGAATGATGCTAATCGGCAGTTACGCGAGCTGTTGTCTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGa                                                                                                                           <  2:73827/146‑1 (MQ=255)
                          gAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAg                                                                                                                          <  1:1183534/150‑1 (MQ=255)
                          gAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAg                                                                                                                          >  2:392786/1‑150 (MQ=255)
                          gAAGCGTTCGATACCGGAAGATAACGCAAACGCCCAAAGTATCATGGCCGATTTTTCCCAGGCTGTGAATGCTGATTATCGGCAGTGCCGAGCGCGGTTGACGACTTTCCGCCTGCCGCTGCAGCCGGCGGGGCTCCCCTCCGCATTGAg                                                                                                                          >  2:196757/1‑150 (MQ=255)
                            aGCGTTCGATACCGGAAGATAACGCAACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAggg                                                                                                                        <  1:234370/150‑1 (MQ=255)
                             gCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGa                                                                                                                       >  2:1036953/1‑150 (MQ=255)
                               gTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGaaa                                                                                                                     >  2:527796/1‑150 (MQ=255)
                                 tCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATg                                                                                                                   >  1:929109/1‑150 (MQ=255)
                                  cGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATgg                                                                                                                  >  2:919781/1‑149 (MQ=255)
                                  cGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGc                                                                                                                  <  1:665000/150‑1 (MQ=255)
                                    aTACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTg                                                                                                                >  1:774002/1‑150 (MQ=255)
                                      aCCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGa                                                                                                              >  1:19028/1‑150 (MQ=255)
                                      aCCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGa                                                                                                              >  2:957784/1‑150 (MQ=255)
                                      aCCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGa                                                                                                              >  2:420027/1‑150 (MQ=255)
                                       ccGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGCCGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGAt                                                                                                             >  2:1232197/1‑150 (MQ=255)
                                            aGATAACGCCACCGCACAAAGTATCATGGCCGATATTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGtt                                                                                                        <  2:531109/150‑1 (MQ=255)
                                              aTAACGCCACCGCACACAGTATCATGGCCGTATTATCCCCGGCGGTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTAc                                                                                                      <  2:94851/150‑1 (MQ=255)
                                                aaCGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACaa                                                                                                    <  2:48197/150‑1 (MQ=255)
                                                aaCGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTAAGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACaa                                                                                                    >  1:131253/1‑150 (MQ=255)
                                                         gCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAAcc                                                                                           <  1:324147/150‑1 (MQ=255)
                                                         gCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAAcc                                                                                           >  1:408672/1‑150 (MQ=255)
                                                           acaAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAg                                                                                         >  1:1174540/1‑150 (MQ=255)
                                                           acaAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAg                                                                                         <  1:691066/150‑1 (MQ=255)
                                                               aGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTAGAAAATCAAACCAGCGcc                                                                                     <  1:130898/150‑1 (MQ=255)
                                                                 tATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCaa                                                                                   >  2:41446/1‑150 (MQ=255)
                                                                  aTCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCaaa                                                                                  >  1:936372/1‑150 (MQ=255)
                                                                  aTCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCaaa                                                                                  <  2:194731/150‑1 (MQ=255)
                                                                      tGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTgc                                                                              >  2:291043/1‑149 (MQ=255)
                                                                       ggCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGAc                                                                             >  1:931465/1‑150 (MQ=255)
                                                                         ccGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGAccc                                                                           <  1:456158/150‑1 (MQ=255)
                                                                                ttCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCACCAAACTGACCCTCGACTg                                                                    <  1:1232197/150‑1 (MQ=255)
                                                                                 tCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTTGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGc                                                                   <  2:1090534/150‑1 (MQ=255)
                                                                                 tCCCAGGCGGTGAATGATGCGTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGc                                                                   <  1:232537/150‑1 (MQ=255)
                                                                                      ggCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGCTCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCt                                                              >  1:202620/1‑150 (MQ=255)
                                                                                      ggCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCt                                                              >  1:22348/1‑150 (MQ=255)
                                                                                       gCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTg                                                             <  1:946274/150‑1 (MQ=255)
                                                                                        cGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAACCCAGCGCCAAACTGACCATCGACTGCCGTCTGc                                                            >  2:64899/1‑150 (MQ=255)
                                                                                        cGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGc                                                            <  1:1039111/150‑1 (MQ=255)
                                                                                         gTTGAATGATTCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGAAGCTGCAGCAGGCGGATCTCCCCTCCGCAGTGAGGGAAATGATGGATACGTTACAAAATCAAACCAGCGCCAACCTGACCCTCTCCTGCCGTCTGcc                                                           >  2:621984/1‑150 (MQ=255)
                                                                                         gTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGCCTGCCGTCTGcc                                                           >  1:887274/1‑150 (MQ=255)
                                                                                           tGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCaa                                                         <  1:385972/150‑1 (MQ=255)
                                                                                            gAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACACTCGACTGCCGTCTGCCAAc                                                        >  1:650744/1‑150 (MQ=255)
                                                                                            gAATGATGCTTATCGGCAGTTACGCGAGCTGGTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAAc                                                        <  1:500279/150‑1 (MQ=255)
                                                                                             aaTGTTGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAAcc                                                       <  2:420944/150‑1 (MQ=255)
                                                                                                gatgCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTg                                                    >  1:1031350/1‑150 (MQ=255)
                                                                                                  tgCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCACGACTGCCGTCTGCCAACCCTgcc                                                  >  2:864212/1‑148 (MQ=255)
                                                                                                   gCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCa                                                 >  2:537504/1‑150 (MQ=255)
                                                                                                      tATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTg                                              <  1:22792/150‑1 (MQ=255)
                                                                                                         cGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCAGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGAt                                           <  2:349/150‑1 (MQ=255)
                                                                                                          ggCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGCCCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATg                                          >  1:725259/1‑150 (MQ=255)
                                                                                                           gCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGCCCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATgc                                         >  2:829052/1‑150 (MQ=255)
                                                                                                           gCAGTTACGCGAGCTGGTGACTACTTTCCGCCTGACCCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATgc                                         <  2:863601/150‑1 (MQ=255)
                                                                                                                   gcgAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGc                                 >  2:724833/1‑150 (MQ=255)
                                                                                                                   gcgAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGc                                 <  1:561776/150‑1 (MQ=255)
                                                                                                                    cgAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCCGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTGACAAAATCAAACCAGCGCCACACTGACCCTCGCCAGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCa                                >  2:837992/1‑150 (MQ=255)
                                                                                                                      aGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAgg                              >  2:908202/1‑150 (MQ=255)
                                                                                                                        cTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGCCCCTCGCCTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTg                            >  2:872806/1‑150 (MQ=255)
                                                                                                                        cTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTg                            <  1:1188122/150‑1 (MQ=255)
                                                                                                                        cTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACACTCGACTGCAGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTg                            >  1:653905/1‑150 (MQ=255)
                                                                                                                            tGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCAtt                        >  2:141460/1‑150 (MQ=255)
                                                                                                                             gACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGGATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCCAACTGCCCCTCGACTGGCGTCTGCCAACCCTGGCTCTGGATGCGCAAATGCGGGTGCAttt                       >  1:1130782/1‑150 (MQ=255)
                                                                                                                               ctactTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATttgt                     <  2:440646/150‑1 (MQ=255)
                                                                                                                               ctactTTCCGCCTGACGCGGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCACCTGCATttgc                     <  1:109468/150‑2 (MQ=255)
                                                                                                                                tactTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATttgtt                    <  1:504267/150‑1 (MQ=255)
                                                                                                                                tactTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATttgtt                    <  2:106472/150‑1 (MQ=255)
                                                                                                                                  ctTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGc                  >  2:174406/1‑150 (MQ=255)
                                                                                                                                   tttCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCa                 >  2:1036563/1‑150 (MQ=255)
                                                                                                                                    atCCGCCTGCTGCAGCGTCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACAAGCGCCAAACTGACCCTCGACTGCCGTCTGCCCACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCaa                >  2:798853/2‑150 (MQ=255)
                                                                                                                                     tCCGCCTGCTGCAGCGTCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCaaa               >  2:322732/1‑150 (MQ=255)
                                                                                                                                      ccGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAat              >  1:673750/1‑150 (MQ=255)
                                                                                                                                       cGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCCGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAatt             >  2:1101660/1‑150 (MQ=255)
                                                                                                                                       cGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACACTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAatt             >  2:128859/1‑150 (MQ=255)
                                                                                                                                        gCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGGCCACCCTGGCACTGGTGGCGCTAATGGAGGTGCAGTTGTGGCattttt            >  2:1212636/1‑145 (MQ=255)
                                                                                                                                           tGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTc         >  2:1260724/1‑150 (MQ=255)
                                                                                                                                            gACGCGGCAGCAGGCGGATCTCCCCTCCGCTATGAGGGAAATGCTGGATACGTTACAAAATCAACCCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTcg        <  1:1146808/150‑1 (MQ=255)
                                                                                                                                             aCGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCGGCGCCAAACTGACCCTCAACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTcgc       <  2:1115406/150‑1 (MQ=255)
                                                                                                                                                 tGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAg   <  1:39223/150‑1 (MQ=255)
                                                                                                                                                 tGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAg   >  1:583176/1‑150 (MQ=255)
                                                                                                                                                  gcagcaGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGc  >  2:390105/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
TACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGC  >  NC_000913/2586865‑2587160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: