Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,705,871 A→T 5.4% V418V (GTT→GTA lepA ← back‑translocating elongation factor EF4, GTPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,705,8710AT 5.4% 232.5 / 2.3 84V418V (GTT→GTAlepAback‑translocating elongation factor EF4, GTPase
Reads supporting (aligned to +/- strand):  ref base A (44/35);  new base T (2/3);  total (46/38)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGG  >  NC_000913/2705722‑2706019
                                                                                                                                                     |                                                                                                                                                    
agagACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGtaata                                                                                                                                                      >  2:771616/1‑150 (MQ=255)
  agaCGCATAACCACGCGAGGTAGCTTTCAGGCGATCGAAGAAGTCGAGCCCCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAAc                                                                                                                                                    >  1:681309/1‑150 (MQ=255)
  agaCGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAAc                                                                                                                                                    >  2:1259716/1‑150 (MQ=255)
     cGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGtt                                                                                                                                                 >  2:482880/1‑150 (MQ=255)
       cATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGc                                                                                                                                               >  1:988288/1‑150 (MQ=255)
        aTAACCACGCGAGGTAGATTTCCGGCGATCGAAGCAGTCGAGCACCACTTCCGCCATCGGGAGCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGcc                                                                                                                                              <  2:56352/150‑1 (MQ=255)
           aCCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTTATTACGTTgtgcgt                                                                                                                                           <  2:1120566/150‑6 (MQ=255)
             cACGCGAGGGTGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCTTCGGTATCTCGTACGTCAGCGCCACCTGATTACCGTTGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTTATTACGTTgtgcgtag                                                                                                                                         <  2:271376/150‑8 (MQ=255)
              aCGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGata                                                                                                                                        >  1:320255/1‑150 (MQ=255)
              aCGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCACCGCCACCTGATAACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGata                                                                                                                                        >  2:564581/1‑150 (MQ=255)
                gcgAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATg                                                                                                                                      <  2:492916/150‑1 (MQ=255)
                gcgAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATg                                                                                                                                      <  2:1048525/150‑1 (MQ=255)
                gcgAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGATTATCTACGCACAACGTAATAACGTTGCCGAGATATg                                                                                                                                      >  1:307635/1‑150 (MQ=255)
                   aGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCAGAGATATGCCt                                                                                                                                   >  1:827079/1‑150 (MQ=255)
                    ggTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTg                                                                                                                                  >  2:1041041/1‑150 (MQ=255)
                    ggTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTg                                                                                                                                  >  2:698895/1‑150 (MQ=255)
                       aGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCgg                                                                                                                               >  1:624505/1‑150 (MQ=255)
                       aGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCgg                                                                                                                               <  1:1092221/150‑1 (MQ=255)
                         aattgaGGCGATCGTAGAAGTCGACCACCACTCCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCTTGGTAAACCATATTCGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATATCGTTGCCGAGTTATGCCTGCGgca                                                                                                                             <  2:275399/145‑1 (MQ=255)
                         aTTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCTGAGATATGCCTGCGgca                                                                                                                             <  1:1178038/150‑1 (MQ=255)
                           ttCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGgcagc                                                                                                                           <  2:730438/150‑1 (MQ=255)
                             cAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGgcagcag                                                                                                                         >  1:470847/1‑150 (MQ=255)
                               ggCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGgcagcagca                                                                                                                       <  1:736299/150‑1 (MQ=255)
                               ggCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGgcagcagca                                                                                                                       >  1:541401/1‑150 (MQ=255)
                               ggCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGgcagcagca                                                                                                                       <  2:349315/150‑1 (MQ=255)
                                gCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCAt                                                                                                                      >  1:84768/1‑150 (MQ=255)
                                 cGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGCTCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCAtg                                                                                                                     >  1:1242259/1‑150 (MQ=255)
                                 cGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCAtg                                                                                                                     >  1:1193517/1‑150 (MQ=255)
                                 cGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCAtg                                                                                                                     >  1:289073/1‑150 (MQ=255)
                                     cGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGac                                                                                                                 >  2:289815/1‑150 (MQ=255)
                                       aagaagTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGacac                                                                                                               >  1:522390/1‑150 (MQ=255)
                                          aagTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACCACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACActcg                                                                                                            >  1:987150/1‑149 (MQ=255)
                                           agTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTg                                                                                                           >  2:1140922/1‑150 (MQ=255)
                                           agTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTg                                                                                                           <  2:418888/150‑1 (MQ=255)
                                           agTCGACCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGGATTTCTACGCACAACGAAATAACGTTGCCGAGATATTCCTGCGGCCGCAGCATGTGACACTCTg                                                                                                           >  1:153762/1‑150 (MQ=255)
                                            gTCGATCACCACTTCAGCCATCTGGATCTCGTACGTCAGCGCCACCTTATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGc                                                                                                          <  2:529846/150‑1 (MQ=255)
                                            gTCGAGCCCCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCAACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGc                                                                                                          <  2:534299/150‑1 (MQ=255)
                                            gTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGc                                                                                                          <  2:1312180/150‑1 (MQ=255)
                                             tCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCa                                                                                                         <  2:1265588/150‑1 (MQ=255)
                                             gcGAGCAACACTTCCGCCATCGGGCGCTCGTACGTCATCTCCACCTGCTGAACGTGGTAAACCATATTGGTCTGCACGACGCGGTTTTCTACGCACATCGTAATAACGTTGCCGATATATGACTGCGGCAGCAGCATGTGACACTCTGCa                                                                                                         <  2:1012028/149‑1 (MQ=255)
                                              cGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGCTTAGCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCCAAACGTAATAACGTTGCCGAGATATTCCTGCGGCAGCAGCATGTGACACTCTGCaa                                                                                                        >  1:621302/1‑150 (MQ=255)
                                                 gCACCACTTCCGCCATCGGGATCTCGTACGTAAGCTCCACCTGATTACCGTGGTAAACCATATTGGTCTGAACGCCGCGTTTTTCTACGAACAACGTAAAAACCTAGCCGAGATATGCCTGCGGCAGCAGAAACTGACCCACAGCAATCg                                                                                                     >  1:937376/1‑150 (MQ=255)
                                                    ccacTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCt                                                                                                  <  2:367059/150‑1 (MQ=255)
                                                     cacTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTc                                                                                                 <  1:1111340/150‑1 (MQ=255)
                                                      acTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTcg                                                                                                <  2:738119/150‑1 (MQ=255)
                                                        ttCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTcgcg                                                                                              <  1:743799/150‑1 (MQ=255)
                                                           cGCCATCGGGATCTCGTACGTAAGCGCCACCTTTTTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAg                                                                                           <  2:827079/150‑1 (MQ=255)
                                                             ccATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGtt                                                                                         <  2:162642/150‑1 (MQ=255)
                                                                        tcGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTa                                                                              >  1:741710/1‑150 (MQ=255)
                                                                         ggTACGTTAGCGCCACTTGATTACCGTGGTAAAAGATATTGGTCTTCTAGCCGCGTTTTTCTAAGCACAACTTAATAAAGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTAt                                                                             <  1:932446/149‑1 (MQ=255)
                                                                          gTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTAtt                                                                            <  2:84768/150‑1 (MQ=255)
                                                                             cGTCAGCGACACCTGATTAGCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTAc                                                                         <  2:96946/150‑1 (MQ=255)
                                                                               tCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCg                                                                       <  2:883107/150‑1 (MQ=255)
                                                                                   cgcCACCTGTTTTCCGGGGTAAATCATATTGCTCTTCACGCCTGGTGTTTCTACGCACAACGTATTAACGTTGCCGAGATATGCCTGCGGCTGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTTTTTACCgcagg                                                                   <  2:788809/150‑1 (MQ=255)
                                                                                     ccACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGACGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCgcaggca                                                                 >  2:726298/1‑150 (MQ=255)
                                                                                         cTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCAGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCtt                                                             >  1:1241505/1‑150 (MQ=255)
                                                                                            aTTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCGTGGa                                                          >  2:668431/1‑150 (MQ=255)
                                                                                              tACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATg                                                        <  1:619462/150‑1 (MQ=255)
                                                                                                 cGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGGTGCCGAGATATGCCTGCGGCAGCAGCATGTGACGCTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGc                                                     <  2:923205/150‑1 (MQ=255)
                                                                                                  gTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCt                                                    >  2:255082/1‑150 (MQ=255)
                                                                                                   tGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACTCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTg                                                   >  1:503593/1‑150 (MQ=255)
                                                                                                     gTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTc                                                 <  2:988288/150‑1 (MQ=255)
                                                                                                       aaaCCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGa                                               >  2:1266829/1‑150 (MQ=255)
                                                                                                            atatTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAg                                          <  2:41699/150‑1 (MQ=255)
                                                                                                                  gTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACt                                    >  1:943734/1‑150 (MQ=255)
                                                                                                                  gTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACt                                    <  2:71273/150‑1 (MQ=255)
                                                                                                                     tGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGAAGGCATCTTGGATTGGCTGTAGACGTAGATAAATtct                                 >  2:638296/1‑150 (MQ=255)
                                                                                                                          gccgcGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGGAGATGTTATTTACAGCAGGCAGCTTGGATGGGCTGTCGACGTAGATACCTTCTCTTGa                            >  1:1197747/1‑150 (MQ=255)
                                                                                                                           ccgcGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTTACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAAATTCTCTTGAc                           >  2:301037/1‑150 (MQ=255)
                                                                                                                              cgTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTg                        >  1:610258/1‑150 (MQ=255)
                                                                                                                               gTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTgg                       <  1:255082/150‑1 (MQ=255)
                                                                                                                                tttttCTACGCACAACGTAATTACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGt                      <  2:541574/150‑1 (MQ=255)
                                                                                                                                   ttCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTc                   <  1:482880/150‑1 (MQ=255)
                                                                                                                                    tCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTCACACTCTGCAATCGGCTCGCGCAGTTCGAAGATGTTATATACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTACTCTTGACGTGGTTTCa                  >  2:464185/1‑150 (MQ=255)
                                                                                                                                      tACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAAc                <  2:1065762/150‑1 (MQ=255)
                                                                                                                                        cGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGGTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCTACtt              >  1:1088388/1‑150 (MQ=255)
                                                                                                                                         gCACAACGTAATACCGTTGCCGAGATATGCCTGCGGCAGCAGGATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCCGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACtta             >  1:401883/1‑149 (MQ=255)
                                                                                                                                         gCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTACATGTTAGTTACCGCACGCAGCTTGCATGGGCTGTCGACTGAGATAACTTCTCTTGACGTGGATTCAACTTc             >  1:72501/1‑150 (MQ=255)
                                                                                                                                         cggcaACGTTATTACGTTGTCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTc             >  1:122938/4‑150 (MQ=255)
                                                                                                                                          ggcaACGTTATTACGTTGTCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCa            >  1:100413/3‑150 (MQ=255)
                                                                                                                                          cacaACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCa            >  1:1306296/1‑150 (MQ=255)
                                                                                                                                             aaCGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATac         <  2:1153505/150‑1 (MQ=255)
                                                                                                                                              aCGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATaca        >  2:716818/1‑150 (MQ=255)
                                                                                                                                              aCGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATaca        >  1:1151784/1‑150 (MQ=255)
                                                                                                                                                gTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACt      >  1:1261645/1‑150 (MQ=255)
                                                                                                                                                    aaaCGTTGCCGAGATATGCCTGCGGCAGAAGCATGTGAAACTCAGCAATCGCCACGCGCAGTTCATAGATGATATTTACCGCAAGAAGCTTGGATGGGCTGTCTACCTAGATAAGTTCTCATGACGTGATTTCAACTTAATACACTACgg  >  2:156838/2‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AGAGACGCATAACCACGCGAGGTAGATTTCAGGCGATCGAAGAAGTCGAGCACCACTTCCGCCATCGGGATCTCGTACGTCAGCGCCACCTGATTACCGTGGTAAACCATATTGGTCTGCACGCCGCGTTTTTCTACGCACAACGTAATAACGTTGCCGAGATATGCCTGCGGCAGCAGCATGTGACACTCTGCAATCGGCTCGCGCAGTTCGTAGATGTTATTTACCGCAGGCAGCTTGGATGGGCTGTCGACGTAGATAACTTCTCTTGACGTGGTTTCAACTTCATACACTACGG  >  NC_000913/2705722‑2706019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: