Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,814,174 T→A 7.6% intergenic (+20/+44) emrB → / ← luxS multidrug efflux system protein/S‑ribosylhomocysteine lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,814,1740TA 7.6% 288.6 / 10.2 102intergenic (+20/+44)emrB/luxSmultidrug efflux system protein/S‑ribosylhomocysteine lyase
Reads supporting (aligned to +/- strand):  ref base T (48/46);  new base A (2/6);  total (50/52)
Fisher's exact test for biased strand distribution p-value = 2.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.09e-01

Only 100 of 106 total aligned reads displayed.

TCAGGGGCTGATTATTTCCGCCAATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCC  >  NC_000913/2814025‑2814323
                                                                                                                                                     |                                                                                                                                                     
tCAGGGGCTGATTATTTCCGCCAATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCAt                                                                                                                                                       >  1:1080414/1‑150 (MQ=255)
  tgggtCTGATTATTTCCGCCAATGAGATCTTCTGGATGGCCGCAGGGATATTCCTCGTCGTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCAttt                                                                                                                                                     <  1:274150/149‑1 (MQ=255)
   ggggCTGATTATTTCCGCCAATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTg                                                                                                                                                    <  2:57026/150‑1 (MQ=255)
      gcggATTATTTCCGCCAATGCGATCTTCTTGATGGCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAAc                                                                                                                                                 <  2:217770/147‑1 (MQ=255)
       cTGATTATTTCCGCCAATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACt                                                                                                                                                >  1:181687/1‑150 (MQ=255)
          attattTCCGCCAATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGGTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGc                                                                                                                                             <  2:1132495/150‑1 (MQ=255)
           ttattTCCGCCAATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCt                                                                                                                                            <  2:1211241/150‑1 (MQ=255)
           ttattTCCGCCAATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCt                                                                                                                                            <  1:505116/150‑1 (MQ=255)
           ttattTCCGCCAATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCAAATGAACTGGCt                                                                                                                                            <  2:98060/150‑1 (MQ=255)
           ttattTCCGCCAATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCAAATGAACTGGCt                                                                                                                                            <  1:434240/150‑1 (MQ=255)
            tattTCCGCCAATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCAAATGAACTGGCtt                                                                                                                                           <  1:697977/150‑1 (MQ=255)
             attTCCGCCAATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCttt                                                                                                                                          >  2:1274602/1‑150 (MQ=255)
              tttCCGCCAATGAGATCTTCTGGATGTCAGCCGGGATATTCCGCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCAAATGAACTGGCttag                                                                                                                                         <  1:1074242/150‑3 (MQ=255)
               ttCCGCCAATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCAAATGAACTGGCttagt                                                                                                                                        <  1:1276633/150‑4 (MQ=255)
                     cAATGAGATCTTCTGGATGTCAGCCGGGACATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCaatt                                                                                                                                  <  2:969364/150‑1 (MQ=255)
                        tGAGTTCTTCTGGATGTCAGCCTGGATATTACTCGTCCTGCTGGTGCTGGTGTGTTTTTCTAAATCGCCATTTGGATCATGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCaattaat                                                                                                                               <  2:869677/150‑1 (MQ=255)
                        tGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCaattaat                                                                                                                               >  2:712078/1‑150 (MQ=255)
                              cttctGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGaa                                                                                                                         >  1:1251134/1‑150 (MQ=255)
                               ttctGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAg                                                                                                                        >  2:865167/1‑150 (MQ=255)
                                    gATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGt                                                                                                                   <  2:627664/150‑1 (MQ=255)
                                          aGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTg                                                                                                             <  1:229025/150‑1 (MQ=255)
                                           gCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCGATTTGGCTCAGGTGGCGGCGGCGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGa                                                                                                            >  1:661502/1‑150 (MQ=255)
                                           gCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGa                                                                                                            >  1:763475/1‑150 (MQ=255)
                                              gggATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTa                                                                                                         <  2:1087953/150‑1 (MQ=255)
                                              gggATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTa                                                                                                         >  2:777267/1‑150 (MQ=255)
                                               ggATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAg                                                                                                        >  2:1002221/1‑150 (MQ=255)
                                               ggATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAg                                                                                                        >  2:897192/1‑150 (MQ=255)
                                                gATATTCATCGTCCTGATGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGCCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTCTGAAGATAGTTTACTGACTAGa                                                                                                       >  2:406934/1‑150 (MQ=255)
                                                  tatTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGAtg                                                                                                     >  1:117446/1‑150 (MQ=255)
                                                    ttCCTCTTCCTGCTGTGGCTGGTGTGGTTTGCTAAACCGGCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACATCTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGAtgtg                                                                                                   <  1:722111/150‑1 (MQ=255)
                                                    ttCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGAtgtg                                                                                                   <  1:146325/150‑1 (MQ=255)
                                                    ttCCACGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGAtgtg                                                                                                   <  2:148450/150‑1 (MQ=255)
                                                       cTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAg                                                                                                <  2:373352/150‑1 (MQ=255)
                                                       cTCGTCCTGCTGGGGCTGGTGGGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAg                                                                                                <  1:654720/150‑1 (MQ=255)
                                                         cGTCCTGCTGGGGCGGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGtt                                                                                              <  1:441889/150‑1 (MQ=255)
                                                          gTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTc                                                                                             >  2:691743/1‑150 (MQ=255)
                                                             ctgctgGGCCTGGTGTGGTTTGCTAACCCGCCCTATGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTTCAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTg                                                                                          <  2:305737/150‑1 (MQ=255)
                                                               gctgGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCa                                                                                        <  2:31338/150‑1 (MQ=255)
                                                                 tgGGGCTGGTGTGGTTTGCTAAACCGCCAATTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAAGTAATTGTGAAGATAGTAAACTGACTAGATGTGCAGTTCCTGCAAc                                                                                      >  1:192007/1‑150 (MQ=255)
                                                                  ggggCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGCGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGCACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACt                                                                                     >  1:101346/1‑150 (MQ=255)
                                                                      cTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTtctc                                                                                 <  2:930932/150‑1 (MQ=255)
                                                                       tGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTctct                                                                                <  1:1067351/150‑1 (MQ=255)
                                                                          tgtgGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTc                                                                             <  2:1127642/150‑1 (MQ=255)
                                                                           gtgGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCg                                                                            >  1:1095700/1‑150 (MQ=255)
                                                                             ggTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGc                                                                          <  1:801896/150‑1 (MQ=255)
                                                                                ttGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGt                                                                       >  1:1030339/1‑150 (MQ=255)
                                                                                 ttcTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTg                                                                      <  1:1158149/148‑1 (MQ=255)
                                                                                 tGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTg                                                                      >  1:759600/1‑150 (MQ=255)
                                                                                 tGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTg                                                                      >  1:482908/1‑150 (MQ=255)
                                                                                     aaaCCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGAGCACAAAGTACAACTAAGCCAGTTCATTTGAACTGGCATTTTTCAATTAATTGTGAAGATATTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCat                                                                  >  1:377900/1‑149 (MQ=255)
                                                                                        ccGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGttc                                                               <  1:891740/150‑1 (MQ=255)
                                                                                        ccGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAAGTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAAGGTCTCTTTCGGCAGTGCCAGttg                                                               >  2:36630/1‑149 (MQ=255)
                                                                                          gCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGttctt                                                             >  2:174713/1‑150 (MQ=255)
                                                                                                tGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGc                                                       <  2:1115220/150‑1 (MQ=255)
                                                                                                 ggCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCt                                                      >  1:913259/1‑150 (MQ=255)
                                                                                                   ggcAGGTGGCGGCGGAGGCGGTGCGCACTCAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGGTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGt                                                    <  1:82101/149‑1 (MQ=255)
                                                                                                    gcAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGtt                                                   >  1:831374/1‑150 (MQ=255)
                                                                                                    gcAGGTGGCGGCGGAGGCGGAGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGtt                                                   <  1:174362/150‑1 (MQ=255)
                                                                                                        gTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATg                                               <  1:728380/150‑1 (MQ=255)
                                                                                                        gTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATg                                               >  1:305732/1‑150 (MQ=255)
                                                                                                            cggcggAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTa                                           >  1:636803/1‑150 (MQ=255)
                                                                                                             ggcggAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGATGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTAc                                          >  1:53573/1‑150 (MQ=255)
                                                                                                             ggcggAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTAc                                          >  1:633389/1‑150 (MQ=255)
                                                                                                             ggcggAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTAc                                          <  2:1081085/150‑1 (MQ=255)
                                                                                                              gcggAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACg                                         <  2:958699/150‑1 (MQ=255)
                                                                                                                ggAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTc                                       >  2:491083/1‑150 (MQ=255)
                                                                                                                  aGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCAc                                     >  1:334922/1‑150 (MQ=255)
                                                                                                                     cGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGtt                                  >  1:1012837/1‑150 (MQ=255)
                                                                                                                     cGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGtt                                  >  1:1130615/1‑150 (MQ=255)
                                                                                                                     cGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGtt                                  <  2:181687/150‑1 (MQ=255)
                                                                                                                       gTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTcc                                <  2:612168/150‑1 (MQ=255)
                                                                                                                         gcgcACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAg                              <  2:1095700/150‑1 (MQ=255)
                                                                                                                         gcgcACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAg                              <  2:1174035/150‑1 (MQ=255)
                                                                                                                         gcgcACTAAGTACAACTAAGCAAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAg                              <  2:1210576/150‑1 (MQ=255)
                                                                                                                            cACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAAt                           >  2:315914/1‑150 (MQ=255)
                                                                                                                            cACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCAAGAAt                           >  1:1264620/1‑150 (MQ=255)
                                                                                                                             aCTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATg                          <  2:51267/150‑1 (MQ=255)
                                                                                                                             aCTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGGGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATg                          <  1:143688/150‑1 (MQ=255)
                                                                                                                                  ttACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTAcg                     <  2:1123615/149‑1 (MQ=255)
                                                                                                                                     gaaaaaaGCCAGTTCAAATGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACgcgc                  >  2:436609/6‑150 (MQ=255)
                                                                                                                                     cAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACgcgc                  >  1:938762/1‑150 (MQ=255)
                                                                                                                                       aCTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTAGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCaa                >  2:829575/1‑150 (MQ=255)
                                                                                                                                        cTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAat               <  2:381595/150‑1 (MQ=255)
                                                                                                                                        aaaaGCCAGTTCAAATGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAat               >  2:90615/3‑150 (MQ=255)
                                                                                                                                         tAAGCCAGTTCATTTGAACTGGATTTTTTCAATAAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGACAGTTCATCGTTGCTTTTGATGCGTACGTCTCGTTCCAGAATGCTACGCGCAata              <  2:644503/150‑1 (MQ=255)
                                                                                                                                            gCCAGTTCTATTGAACGGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATTTGCAGTTCCTGCAATTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGCTGATGCGTACGTCACTTTCCAGAATGCTACGCGCAATATcc           <  1:176165/150‑1 (MQ=255)
                                                                                                                                            gCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATcc           <  2:1153670/150‑1 (MQ=255)
                                                                                                                                            gCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATcc           <  1:777267/150‑1 (MQ=255)
                                                                                                                                             ccAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCt          <  1:1078910/150‑1 (MQ=255)
                                                                                                                                              cAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTACATGTGCAGTTCCTGCAACTTCTCTTTCGGAAGTGCCAGTTCTTCGTTGCTGTTTATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTg         >  2:550147/1‑150 (MQ=255)
                                                                                                                                               aGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCATGCAACTTCTATTTAGGAAGTGACAGTTCTTCGTTGATGTTGATGCGTACGTCACGTTCAAGAATGCTAGGCGCAATATCCTgc        >  1:45168/1‑150 (MQ=255)
                                                                                                                                                  tCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCt     >  2:297658/1‑150 (MQ=255)
                                                                                                                                                   cATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCTCtt    >  1:826554/1‑150 (MQ=255)
                                                                                                                                                   cATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCtt    >  1:835130/1‑150 (MQ=255)
                                                                                                                                                   cATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCtt    <  1:1274602/150‑1 (MQ=255)
                                                                                                                                                   cATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCATGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGGTGCTGTTGCTGCGTACGTCCCGTTCCAGAATGCTACGCGCACTATCCTGCGCtt    >  2:653707/1‑150 (MQ=255)
                                                                                                                                                   cATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGAGCAGATCCTGCAACTTCTCTTTCGGAAGTGACAGTTATTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGAtt    >  1:1197213/1‑150 (MQ=255)
                                                                                                                                                    aTTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCGTCGTTGCGGTTGAGGCGTACTTCACGTTCCCGAATGCTACGCGCAAGATCCTGCGCTTc   >  2:103460/1‑150 (MQ=255)
                                                                                                                                                     tttGTACTGGCTTTTTTCTATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTACTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTcc  <  2:1153174/150‑1 (MQ=255)
                                                                                                                                                     tttGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTTTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTcc  <  1:1152972/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCAGGGGCTGATTATTTCCGCCAATGAGATCTTCTGGATGTCAGCCGGGATATTCCTCGTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCC  >  NC_000913/2814025‑2814323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: