Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,931,831 A→T 5.2% intergenic (+21/+34) ygdG → / ← fucO Ssb‑binding protein, misidentified as ExoIX/L‑1,2‑propanediol oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,931,8310AT 5.2% 200.9 / 2.6 77intergenic (+21/+34)ygdG/fucOSsb‑binding protein, misidentified as ExoIX/L‑1,2‑propanediol oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (31/41);  new base T (2/2);  total (34/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.92e-01

TTGCCGAAAAGTGGCGCAAAAAATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGAATGTCTTCCTTG  >  NC_000913/2931683‑2931979
                                                                                                                                                    |                                                                                                                                                    
ttGCCGAATAGTGGCGGAAAAAATTAGAAACCCATAAAGAGATGGCGGTTCTGTGCCGCGATATTGCGCGCTTACATACCGTTTTGCATATCGCCGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCaa                                                                                                                                                     <  2:535514/150‑1 (MQ=255)
  gCCGAAAAGTGGCGCAAAAAATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAAc                                                                                                                                                   >  1:181982/1‑150 (MQ=255)
    cGAAAAGTGGCGCAAAAAATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGCCGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAAcgt                                                                                                                                                 >  1:915965/1‑150 (MQ=255)
    cGAAAAGTGGCGCAAAAAATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATTCGCCGCAAAcgt                                                                                                                                                 >  1:143808/1‑150 (MQ=255)
    cGAAAAGTGGCGCAAAAAATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCCGATACGCCGCAAAcgt                                                                                                                                                 >  1:1275535/1‑150 (MQ=255)
    cGAAAAGTGGCGCAAAAAATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGAAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAAcgt                                                                                                                                                 >  1:768540/1‑150 (MQ=255)
     taaaaGTGGCGCAAAAAATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAAcgtc                                                                                                                                                <  1:1081731/149‑1 (MQ=255)
     gAAAAGTGGCGCAAAAAATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAAcgtc                                                                                                                                                <  1:377384/150‑1 (MQ=255)
      aaaaGTGGCGCAAAAAATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAAcgtcg                                                                                                                                               <  1:499197/150‑1 (MQ=255)
       aaaGTGGCGCAAAAAATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAAcgtcgt                                                                                                                                              <  2:397790/150‑1 (MQ=255)
             gcgcAAAAAATTAGAAACCCATAAAGAGATGGCGGTTCTGTGCCGCGATATTGCCCGCTTACAAACCGAATTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGACGTTTGCGGCGTATCCgg                                                                                                                                        <  2:933253/150‑1 (MQ=255)
              cgcAAAAAATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAAAGTCGTATCCGGc                                                                                                                                       >  1:33171/1‑150 (MQ=255)
              cgcAAAAAATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGACGTTTGCGGCGTATCCGGc                                                                                                                                       <  1:373850/150‑1 (MQ=255)
               gcAAAAAATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCa                                                                                                                                      <  2:1210702/150‑1 (MQ=255)
                    aaaTTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAACCCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCAGCAAACGTCGTATCCTGCATGATc                                                                                                                                 >  1:888709/1‑150 (MQ=255)
                      aTTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATcac                                                                                                                               <  2:705746/150‑1 (MQ=255)
                              cccATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAgcgc                                                                                                                       <  1:937721/150‑1 (MQ=255)
                               ccATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGCCTAAGCCGCAAACCTCGTATGCGGGAATATAACATCACCGCa                                                                                                                      >  2:428253/1‑150 (MQ=255)
                                 aTAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCTGCAAACGTCGTATCCGGCATTATCACATCAGCGCAtt                                                                                                                    <  2:1290115/150‑1 (MQ=255)
                                  tAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTTCAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCAttt                                                                                                                   >  1:898817/1‑150 (MQ=255)
                                  tAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCAttt                                                                                                                   <  1:464936/150‑1 (MQ=255)
                                    aaGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTAc                                                                                                                 >  2:936825/1‑150 (MQ=255)
                                     agagATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTAcc                                                                                                                <  2:33958/150‑1 (MQ=255)
                                     agagATGGCGGTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTAcc                                                                                                                <  2:599250/150‑1 (MQ=255)
                                       agaTGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAg                                                                                                              <  1:1254928/150‑1 (MQ=255)
                                         aTGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGc                                                                                                            <  1:276865/150‑1 (MQ=255)
                                                       ccGCGATATTGCCCGCTTCCAAACCGATTTGCATATCGACGGAAATTTACAGCAATTGCGGGTGGTACGGTAACGGCGAGCCCGGTAAGCCGCAAAAGTCGTATACGCCAATATCACAACAGCCCATTAACCAGGCGGTATTGGAAAGca                                                                                              >  2:319614/1‑149 (MQ=255)
                                                       ccGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAACCGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTTTGGTAAAGct                                                                                              >  2:23432/1‑150 (MQ=255)
                                                         gcgATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGctct                                                                                            <  1:574753/150‑1 (MQ=255)
                                                               ttGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGATCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAata                                                                                      <  2:583473/150‑1 (MQ=255)
                                                                 gCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGCTACGCCGCCAACCGCGGATACGGCAGTATCACAACAGCCCATTTACCCCGCGGTTATGTAAAGCGCGAGAatagc                                                                                    >  2:450705/1‑148 (MQ=255)
                                                                    cGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGCATAAGCCGCAAACGTCGTATACCGCATTATAACATCATCGCATTTACCAGGCGGTATTGTAAAACTCTAAAAAATACta                                                                                 >  2:643984/1‑149 (MQ=255)
                                                                     gCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCa                                                                                >  1:910610/1‑150 (MQ=255)
                                                                       ttACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAg                                                                              <  1:795599/150‑1 (MQ=255)
                                                                          cAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCCTTATCACATCAGCGCATTTTCCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGt                                                                           >  1:671793/1‑150 (MQ=255)
                                                                          cAAACCGATTTCCATATCGACGGCAATTTACAGCAATTGCGGGTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGt                                                                           <  2:336434/150‑1 (MQ=255)
                                                                             aCCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATAGGCCGCAAACGGCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGc                                                                        <  2:882738/150‑1 (MQ=255)
                                                                              ccGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCt                                                                       >  1:21562/1‑150 (MQ=255)
                                                                              ccGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGAAAACGTCGTATCCGGCATTATCCCCTAAGCGCATTTACCCGACTGTATGGAAAAGCTCTACAAAATCCTCAAGAGATGca                                                                       >  1:155011/1‑149 (MQ=255)
                                                                                taTTTGCATATCGACGGCAATTTACAGCAATTTCGTTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTTCCAGGAGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGGTTc                                                                     <  1:189935/149‑1 (MQ=255)
                                                                                     gCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCCGATACGCCGCAAACGTCGTATCCGGCATTATGACATCAGCGCATTTACCAGGCGGTATGGTTAACCTCTACAATATCCTCAAGCGTTTCTTAAcgcg                                                                >  1:228901/1‑150 (MQ=255)
                                                                                       atatCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCggg                                                              <  2:145481/150‑1 (MQ=255)
                                                                                       atatCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCggg                                                              <  1:733821/150‑1 (MQ=255)
                                                                                          tCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTg                                                           <  2:803438/150‑1 (MQ=255)
                                                                                             aCGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCGACAATATCCTCAAGCGTTGCTTCACGCGGGTTGccc                                                        >  1:693878/1‑149 (MQ=255)
                                                                                              cGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCCGATACGCCGCAAACGTCGTATACGGGATTATCCCATCAGCCCATTTACCCGGCGGTATGGTAAAGCTCTACAATATGCTCAAGCGTTTCTTAACGCGGGtttgcac                                                       >  2:1235585/1‑145 (MQ=255)
                                                                                               ggCAATTTACAGCAATTGCGGTTGGTAGGGTAACGGCGAGACGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCAcc                                                      <  2:48800/150‑1 (MQ=255)
                                                                                                   aTTTCCAGCAAATGCGGTGGGGACGGAAACGGCGCGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCTGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTa                                                  <  1:1014115/150‑1 (MQ=255)
                                                                                                   aTTTACATCAATTGCGGTTAGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTa                                                  <  2:63611/150‑1 (MQ=255)
                                                                                                   aTTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCCGAAACGCCGCAAACCTCGTTTACGGCAATATAAAAAAAACGCATTTAACAAGCGGGATTGTAAACCAAGACAATATACACAAACCTTTGTTAACGCGGGTTGCCACCCGTa                                                  >  2:1207525/1‑150 (MQ=255)
                                                                                                      tACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACaa                                               >  1:915631/1‑150 (MQ=255)
                                                                                                      tACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATACTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACaa                                               >  2:30834/1‑150 (MQ=255)
                                                                                                             aTTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGACGTATACGGCATTATCACATCAGCGCATTTTCCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAATcatcat                                        >  1:846984/1‑150 (MQ=255)
                                                                                                               tGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATcc                                      <  1:580953/150‑1 (MQ=255)
                                                                                                               tGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATcc                                      <  2:470929/150‑1 (MQ=255)
                                                                                                               tGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATcc                                      <  2:466238/150‑1 (MQ=255)
                                                                                                                gCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCTGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCa                                     <  1:722132/150‑1 (MQ=255)
                                                                                                                gCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCa                                     <  1:483930/150‑1 (MQ=255)
                                                                                                                 cGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAg                                    <  2:181982/150‑1 (MQ=255)
                                                                                                                    ttGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTgc                                 <  1:874547/150‑1 (MQ=255)
                                                                                                                      ggTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTgccg                               <  2:300947/150‑1 (MQ=255)
                                                                                                                        taaggTAACGGCGAGCCTGATACGCAGCAAACGTAGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTgccgcc                             <  2:860985/147‑1 (MQ=255)
                                                                                                                         aCGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCt                            >  2:751977/1‑150 (MQ=255)
                                                                                                                         aCGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCt                            >  1:23697/1‑150 (MQ=255)
                                                                                                                                cGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAg                     <  2:1210793/150‑1 (MQ=255)
                                                                                                                                    gAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGcc                 <  2:910610/150‑1 (MQ=255)
                                                                                                                                     aGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCg                <  1:533309/150‑1 (MQ=255)
                                                                                                                                      gCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCgg               >  1:397721/1‑150 (MQ=255)
                                                                                                                                      gCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCgg               <  2:143808/150‑1 (MQ=255)
                                                                                                                                      gCCGGATACGACGTTTGCGGCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCgg               >  1:1114825/1‑150 (MQ=255)
                                                                                                                                       ccGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGATGCCACCGGTCCAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGa              >  1:193625/1‑150 (MQ=255)
                                                                                                                                       ccGGATACGACGTTTGCGGCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACCACCATCATCCAGTGCCGCCTGCGCCAGTGCCGGa              >  1:956174/1‑150 (MQ=255)
                                                                                                                                        cGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGaa             >  1:1057361/1‑150 (MQ=255)
                                                                                                                                          gATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTAGGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGAATg           <  2:158492/150‑1 (MQ=255)
                                                                                                                                             aCGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGAATGTCt        <  2:985400/150‑1 (MQ=255)
                                                                                                                                                  gcAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCACGCGTTGCTTCGCGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGAATGGCTTCCtt   >  2:528051/1‑150 (MQ=255)
                                                                                                                                                   cAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGAATGTCTTCCTTg  <  1:42532/150‑1 (MQ=255)
                                                                                                                                                   cAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGAATGTCTTCCTTg  >  1:101094/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TTGCCGAAAAGTGGCGCAAAAAATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGAATGTCTTCCTTG  >  NC_000913/2931683‑2931979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: