Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,931,833 A→G 5.2% intergenic (+23/+32) ygdG → / ← fucO Ssb‑binding protein, misidentified as ExoIX/L‑1,2‑propanediol oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,931,8330AG 5.2% 225.9 / 3.2 77intergenic (+23/+32)ygdG/fucOSsb‑binding protein, misidentified as ExoIX/L‑1,2‑propanediol oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (31/41);  new base G (2/2);  total (34/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.31e-01

GCCGAAAAGTGGCGCAAAAAATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGAATGTCTTCCTTGCG  >  NC_000913/2931685‑2931981
                                                                                                                                                    |                                                                                                                                                    
gCCGAAAAGTGGCGCAAAAAATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAAc                                                                                                                                                     >  1:181982/1‑150 (MQ=255)
  cGAAAAGTGGCGCAAAAAATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGCCGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAAcgt                                                                                                                                                   >  1:915965/1‑150 (MQ=255)
  cGAAAAGTGGCGCAAAAAATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATTCGCCGCAAAcgt                                                                                                                                                   >  1:143808/1‑150 (MQ=255)
  cGAAAAGTGGCGCAAAAAATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCCGATACGCCGCAAAcgt                                                                                                                                                   >  1:1275535/1‑150 (MQ=255)
  cGAAAAGTGGCGCAAAAAATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGAAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAAcgt                                                                                                                                                   >  1:768540/1‑150 (MQ=255)
   taaaaGTGGCGCAAAAAATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAAcgtc                                                                                                                                                  <  1:1081731/149‑1 (MQ=255)
   gAAAAGTGGCGCAAAAAATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAAcgtc                                                                                                                                                  <  1:377384/150‑1 (MQ=255)
    aaaaGTGGCGCAAAAAATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAAcgtcg                                                                                                                                                 <  1:499197/150‑1 (MQ=255)
     aaaGTGGCGCAAAAAATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAAcgtcgt                                                                                                                                                <  2:397790/150‑1 (MQ=255)
           gcgcAAAAAATTAGAAACCCATAAAGAGATGGCGGTTCTGTGCCGCGATATTGCCCGCTTACAAACCGAATTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGACGTTTGCGGCGTATCCgg                                                                                                                                          <  2:933253/150‑1 (MQ=255)
            cgcAAAAAATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAAAGTCGTATCCGGc                                                                                                                                         >  1:33171/1‑150 (MQ=255)
            cgcAAAAAATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGACGTTTGCGGCGTATCCGGc                                                                                                                                         <  1:373850/150‑1 (MQ=255)
             gcAAAAAATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCa                                                                                                                                        <  2:1210702/150‑1 (MQ=255)
                  aaaTTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAACCCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCAGCAAACGTCGTATCCTGCATGATc                                                                                                                                   >  1:888709/1‑150 (MQ=255)
                    aTTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATcac                                                                                                                                 <  2:705746/150‑1 (MQ=255)
                            cccATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAgcgc                                                                                                                         <  1:937721/150‑1 (MQ=255)
                             ccATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGCCTAAGCCGCAAACCTCGTATGCGGGAATATAACATCACCGCa                                                                                                                        >  2:428253/1‑150 (MQ=255)
                               aTAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCTGCAAACGTCGTATCCGGCATTATCACATCAGCGCAtt                                                                                                                      <  2:1290115/150‑1 (MQ=255)
                                tAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTTCAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCAttt                                                                                                                     >  1:898817/1‑150 (MQ=255)
                                tAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCAttt                                                                                                                     <  1:464936/150‑1 (MQ=255)
                                  aaGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTAc                                                                                                                   >  2:936825/1‑150 (MQ=255)
                                   agagATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTAcc                                                                                                                  <  2:33958/150‑1 (MQ=255)
                                   agagATGGCGGTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTAcc                                                                                                                  <  2:599250/150‑1 (MQ=255)
                                     agaTGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAg                                                                                                                <  1:1254928/150‑1 (MQ=255)
                                       aTGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGc                                                                                                              <  1:276865/150‑1 (MQ=255)
                                                     ccGCGATATTGCCCGCTTCCAAACCGATTTGCATATCGACGGAAATTTACAGCAATTGCGGGTGGTACGGTAACGGCGAGCCCGGTAAGCCGCAAAAGTCGTATACGCCAATATCACAACAGCCCATTAACCAGGCGGTATTGGAAAGca                                                                                                >  2:319614/1‑149 (MQ=255)
                                                     ccGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAACCGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTTTGGTAAAGct                                                                                                >  2:23432/1‑150 (MQ=255)
                                                       gcgATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGctct                                                                                              <  1:574753/150‑1 (MQ=255)
                                                             ttGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGATCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAata                                                                                        <  2:583473/150‑1 (MQ=255)
                                                               gCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGCTACGCCGCCAACCGCGGATACGGCAGTATCACAACAGCCCATTTACCCCGCGGTTATGTAAAGCGCGAGAatagc                                                                                      >  2:450705/1‑148 (MQ=255)
                                                                  cGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGCATAAGCCGCAAACGTCGTATACCGCATTATAACATCATCGCATTTACCAGGCGGTATTGTAAAACTCTAAAAAATACta                                                                                   >  2:643984/1‑149 (MQ=255)
                                                                   gCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCa                                                                                  >  1:910610/1‑150 (MQ=255)
                                                                     ttACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAg                                                                                <  1:795599/150‑1 (MQ=255)
                                                                        cAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCCTTATCACATCAGCGCATTTTCCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGt                                                                             >  1:671793/1‑150 (MQ=255)
                                                                        cAAACCGATTTCCATATCGACGGCAATTTACAGCAATTGCGGGTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGt                                                                             <  2:336434/150‑1 (MQ=255)
                                                                           aCCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATAGGCCGCAAACGGCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGc                                                                          <  2:882738/150‑1 (MQ=255)
                                                                            ccGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCt                                                                         >  1:21562/1‑150 (MQ=255)
                                                                            ccGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGAAAACGTCGTATCCGGCATTATCCCCTAAGCGCATTTACCCGACTGTATGGAAAAGCTCTACAAAATCCTCAAGAGATGca                                                                         >  1:155011/1‑149 (MQ=255)
                                                                              taTTTGCATATCGACGGCAATTTACAGCAATTTCGTTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTTCCAGGAGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGGTTc                                                                       <  1:189935/149‑1 (MQ=255)
                                                                                   gCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCCGATACGCCGCAAACGTCGTATCCGGCATTATGACATCAGCGCATTTACCAGGCGGTATGGTTAACCTCTACAATATCCTCAAGCGTTTCTTAAcgcg                                                                  >  1:228901/1‑150 (MQ=255)
                                                                                     atatCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCggg                                                                <  2:145481/150‑1 (MQ=255)
                                                                                     atatCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCggg                                                                <  1:733821/150‑1 (MQ=255)
                                                                                        tCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTg                                                             <  2:803438/150‑1 (MQ=255)
                                                                                           aCGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCGACAATATCCTCAAGCGTTGCTTCACGCGGGTTGccc                                                          >  1:693878/1‑149 (MQ=255)
                                                                                            cGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCCGATACGCCGCAAACGTCGTATACGGGATTATCCCATCAGCCCATTTACCCGGCGGTATGGTAAAGCTCTACAATATGCTCAAGCGTTTCTTAACGCGGGtttgcac                                                         >  2:1235585/1‑145 (MQ=255)
                                                                                             ggCAATTTACAGCAATTGCGGTTGGTAGGGTAACGGCGAGACGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCAcc                                                        <  2:48800/150‑1 (MQ=255)
                                                                                                 aTTTCCAGCAAATGCGGTGGGGACGGAAACGGCGCGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCTGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTa                                                    <  1:1014115/150‑1 (MQ=255)
                                                                                                 aTTTACATCAATTGCGGTTAGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTa                                                    <  2:63611/150‑1 (MQ=255)
                                                                                                 aTTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCCGAAACGCCGCAAACCTCGTTTACGGCAATATAAAAAAAACGCATTTAACAAGCGGGATTGTAAACCAAGACAATATACACAAACCTTTGTTAACGCGGGTTGCCACCCGTa                                                    >  2:1207525/1‑150 (MQ=255)
                                                                                                    tACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACaa                                                 >  1:915631/1‑150 (MQ=255)
                                                                                                    tACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATACTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACaa                                                 >  2:30834/1‑150 (MQ=255)
                                                                                                           aTTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGACGTATACGGCATTATCACATCAGCGCATTTTCCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAATcatcat                                          >  1:846984/1‑150 (MQ=255)
                                                                                                             tGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATcc                                        <  1:580953/150‑1 (MQ=255)
                                                                                                             tGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATcc                                        <  2:470929/150‑1 (MQ=255)
                                                                                                             tGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATcc                                        <  2:466238/150‑1 (MQ=255)
                                                                                                              gCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCTGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCa                                       <  1:722132/150‑1 (MQ=255)
                                                                                                              gCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCa                                       <  1:483930/150‑1 (MQ=255)
                                                                                                               cGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAg                                      <  2:181982/150‑1 (MQ=255)
                                                                                                                  ttGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTgc                                   <  1:874547/150‑1 (MQ=255)
                                                                                                                    ggTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTgccg                                 <  2:300947/150‑1 (MQ=255)
                                                                                                                      taaggTAACGGCGAGCCTGATACGCAGCAAACGTAGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTgccgcc                               <  2:860985/147‑1 (MQ=255)
                                                                                                                       aCGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCt                              >  2:751977/1‑150 (MQ=255)
                                                                                                                       aCGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCt                              >  1:23697/1‑150 (MQ=255)
                                                                                                                              cGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAg                       <  2:1210793/150‑1 (MQ=255)
                                                                                                                                  gAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGcc                   <  2:910610/150‑1 (MQ=255)
                                                                                                                                   aGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCg                  <  1:533309/150‑1 (MQ=255)
                                                                                                                                    gCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCgg                 <  2:143808/150‑1 (MQ=255)
                                                                                                                                    gCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCgg                 >  1:397721/1‑150 (MQ=255)
                                                                                                                                    gCCGGATACGACGTTTGCGGCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCgg                 >  1:1114825/1‑150 (MQ=255)
                                                                                                                                     ccGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGATGCCACCGGTCCAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGa                >  1:193625/1‑150 (MQ=255)
                                                                                                                                     ccGGATACGACGTTTGCGGCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACCACCATCATCCAGTGCCGCCTGCGCCAGTGCCGGa                >  1:956174/1‑150 (MQ=255)
                                                                                                                                      cGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGaa               >  1:1057361/1‑150 (MQ=255)
                                                                                                                                        gATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTAGGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGAATg             <  2:158492/150‑1 (MQ=255)
                                                                                                                                           aCGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGAATGTCt          <  2:985400/150‑1 (MQ=255)
                                                                                                                                                gcAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCACGCGTTGCTTCGCGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGAATGGCTTCCtt     >  2:528051/1‑150 (MQ=255)
                                                                                                                                                 cAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGAATGTCTTCCTTg    <  1:42532/150‑1 (MQ=255)
                                                                                                                                                 cAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGAATGTCTTCCTTg    >  1:101094/1‑150 (MQ=255)
                                                                                                                                                   aaCGGCGTATCCGGCATTATCACATCAGCGCATTTCCCAGGCGGTAGGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGGTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGAATGTCTTCCTTGCg  <  1:863319/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCCGAAAAGTGGCGCAAAAAATTAGAAACCCATAAAGAGATGGCGTTTCTGTGCCGCGATATTGCCCGCTTACAAACCGATTTGCATATCGACGGCAATTTACAGCAATTGCGGTTGGTACGGTAACGGCGAGCCGGATACGCCGCAAACGTCGTATCCGGCATTATCACATCAGCGCATTTACCAGGCGGTATGGTAAAGCTCTACAATATCCTCAAGCGTTGCTTCACGCGGGTTGCCACCGGTACAAACATCATCCAGTGCCGCCTGCGCCAGTGCCGGAATGTCTTCCTTGCG  >  NC_000913/2931685‑2931981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: