Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,980,744 A→T 7.0% intergenic (‑109/+20) ygeA ← / ← araE Asp/Glu_racemase family protein/arabinose transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,980,7440AT 7.0% 176.0 / 3.3 70intergenic (‑109/+20)ygeA/araEAsp/Glu_racemase family protein/arabinose transporter
Reads supporting (aligned to +/- strand):  ref base A (32/31);  new base T (2/2);  total (35/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.51e-01

TTCCCAGCTCATTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTA  >  NC_000913/2980597‑2980892
                                                                                                                                                   |                                                                                                                                                    
ttCCCAGCTCATTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGc                                                                                                                                                    >  2:565407/1‑150 (MQ=255)
 tCCCAGCTCATTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTAGAGca                                                                                                                                                   >  2:1231199/1‑150 (MQ=255)
   ccAGCTCATTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCACCAACGCGGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGCAAACAGGGCCGGATGTACCGcacc                                                                                                                                                 >  1:100877/1‑149 (MQ=255)
      gCTCATTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATcc                                                                                                                                              <  1:541551/150‑1 (MQ=255)
      gCTCATACCTCCCAGCAAACCACTTCTTTTCATTCCACGCACCTGATGTGTGCAACCGCAACAAGCCTGAATCAGGAACGAGACAAACGCCTCAACGTCCAAGTGCCCAACCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATcc                                                                                                                                              <  2:1139229/150‑1 (MQ=255)
         cATTCCTCCCAGCAAACCAATTGTGTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCGGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTGCTGTACATCCGGc                                                                                                                                           <  2:273650/150‑1 (MQ=255)
          aTTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTGCTGTACATCCGGcc                                                                                                                                          <  1:1195214/150‑1 (MQ=255)
                cccAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGaa                                                                                                                                    >  1:1280402/1‑150 (MQ=255)
                 ccAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGaaa                                                                                                                                   >  2:174866/1‑150 (MQ=255)
                  cAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAATGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAAt                                                                                                                                  >  1:437986/1‑150 (MQ=255)
                  cAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCATGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACAACCGGCCCGTGAAAt                                                                                                                                  >  1:711277/1‑150 (MQ=255)
                     cAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAg                                                                                                                               >  2:294721/1‑150 (MQ=255)
                     cAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAg                                                                                                                               >  2:1179083/1‑150 (MQ=255)
                               gTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGatat                                                                                                                     >  2:487207/1‑150 (MQ=255)
                                  ttCATTCCTCTCTCCTGATTTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTtc                                                                                                                  <  1:273342/150‑1 (MQ=255)
                                    ctttCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTtctc                                                                                                                <  1:813260/148‑1 (MQ=255)
                                       tCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTTAATAAGGAACTAGACAAACGCCTCAAAGTACAAGTTCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAAc                                                                                                             <  1:64177/150‑1 (MQ=255)
                                       tCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAAc                                                                                                             <  1:711023/150‑1 (MQ=255)
                                        ccTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGCCAAACGCCTCAACGTCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGTCCCGTGAAATCAGCCGCCGATATTTCTCAACt                                                                                                            >  1:94353/1‑150 (MQ=255)
                                           ctctcCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTTCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTtct                                                                                                         <  1:624190/150‑1 (MQ=255)
                                           ctctcCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTtct                                                                                                         >  2:219595/1‑150 (MQ=255)
                                             ctcCTGATGTGTGTTACCGCAACAAGTCTGAAAAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCg                                                                                                       <  1:1060468/150‑1 (MQ=255)
                                               ccTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCACCGGCCAAGTGCCCAATCTCTATTTACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAGTCAGCCGCCGATATTTCTCACCTTcgcggc                                                                                                     >  2:618983/1‑148 (MQ=255)
                                               ccTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGAGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGcc                                                                                                     >  2:59795/1‑150 (MQ=255)
                                                  gATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGc                                                                                                  >  1:152918/1‑150 (MQ=255)
                                                  gATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGc                                                                                                  >  1:1282992/1‑150 (MQ=255)
                                                   aTGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGcc                                                                                                 >  1:1180057/1‑150 (MQ=255)
                                                     gtgtgtTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCATCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCACGTGAAATCAGACGCAGATATTTCTCAACTTCTAGCCTGCAAt                                                                                               >  1:1192088/1‑150 (MQ=255)
                                                      tgtgtTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATc                                                                                              >  1:351813/1‑150 (MQ=255)
                                                       gtgtTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCa                                                                                             <  2:355770/150‑1 (MQ=255)
                                                        tgtTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAg                                                                                            >  2:247310/1‑150 (MQ=255)
                                                           tACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAACAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGttt                                                                                         >  2:382975/1‑150 (MQ=255)
                                                           tACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGttt                                                                                         >  2:1280767/1‑150 (MQ=255)
                                                            aCCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTg                                                                                        <  1:1317573/150‑1 (MQ=255)
                                                              cGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCg                                                                                      <  1:36230/150‑1 (MQ=255)
                                                                  acaaGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCCTCAGTTTGCGTTCg                                                                                  >  1:964869/1‑150 (MQ=255)
                                                                                ggAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGt                                                                    <  1:1271004/150‑1 (MQ=255)
                                                                                  accGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATAAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGa                                                                  <  2:208541/148‑1 (MQ=255)
                                                                                   aCGAGACAAACGCCTCTACGGCCAAGTGCCCAATCTCTATAAACGAAAAAAGGGCCGGATGTACAGCACAACCGGCCGGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGAc                                                                 <  2:1188381/150‑1 (MQ=255)
                                                                                    cGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACa                                                                <  2:1282992/150‑1 (MQ=255)
                                                                                      agaCAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACAtt                                                              >  1:1018311/1‑150 (MQ=255)
                                                                                          aaaCGCATCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACCGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTg                                                          <  1:945199/150‑1 (MQ=255)
                                                                                           aaCGCCTCAACGGCCATGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGTTGTACAGCACCTCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCGGCGATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTgg                                                         <  1:849761/150‑1 (MQ=255)
                                                                                                cTCAACGGCCAAGTGCCCAATCTCAATAACCGAAAAAAGGGCAGGATTTCCAGCACATCCGGCCAGTGAAATAAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCATTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTcc                                                    <  1:1176759/150‑1 (MQ=255)
                                                                                                 tCAACGTCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCg                                                   <  1:514517/150‑1 (MQ=255)
                                                                                                  aaaCGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGAACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCgg                                                  <  1:965721/149‑1 (MQ=255)
                                                                                                        ccAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAg                                            <  2:351813/150‑1 (MQ=255)
                                                                                                         cAAGTGCCCTATCTATATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGc                                           <  2:614054/150‑1 (MQ=255)
                                                                                                           aGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCa                                         >  2:1250795/1‑150 (MQ=255)
                                                                                                           aGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCa                                         <  1:1235270/150‑1 (MQ=255)
                                                                                                           aGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCa                                         <  1:487207/150‑1 (MQ=255)
                                                                                                           aGTGCCCAATATCTATTAACGAAAAAAGGGCCGGATGTACGGCACATCCGGCCCGTGAAATCAGTCGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCa                                         <  2:778936/150‑1 (MQ=255)
                                                                                                              gCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGaa                                      >  2:790960/1‑150 (MQ=255)
                                                                                                                 cAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGt                                   <  1:174866/150‑1 (MQ=255)
                                                                                                                       ctATTAACGAAAAACGGTCCTGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCGCGCCGGCCATCAGTGTGCGTGCGATATGTTCCAGCGTGACATTTTTGGTTTACGGAATGAGCCAGAAAGTAATGcc                             <  2:633005/150‑1 (MQ=255)
                                                                                                                        tATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGccc                            <  1:247310/150‑1 (MQ=255)
                                                                                                                             aCGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACaaa                       >  2:302306/1‑150 (MQ=255)
                                                                                                                              cGAAAAAACGCCCGGATGTACAGCACATCCGGCCAGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATTAGCCAGAAAGTAATGCCCACAAAc                      <  1:358962/150‑1 (MQ=255)
                                                                                                                               gAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACg                     >  2:1125563/1‑150 (MQ=255)
                                                                                                                                       ggCCGGATGTGCTGTACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTc             >  1:1052001/1‑150 (MQ=255)
                                                                                                                                       ggCCGGATGTGCTGTACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCAACAAACGCAATGTTc             >  2:1287741/1‑150 (MQ=255)
                                                                                                                                           ggATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAATTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAgcg         <  1:113202/150‑1 (MQ=255)
                                                                                                                                           ggATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAgcg         <  2:1180057/150‑1 (MQ=255)
                                                                                                                                           ggATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAgcg         >  2:600587/1‑150 (MQ=255)
                                                                                                                                            gATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAgcgc        <  2:152918/150‑1 (MQ=255)
                                                                                                                                              tGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAg      <  1:565407/150‑1 (MQ=255)
                                                                                                                                               gTACAGCACATCCGGCCCGTGAAATCAGAAGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCTgt     >  1:357779/1‑150 (MQ=255)
                                                                                                                                                tACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCAATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCACCCAAACGCAATGTTCAGCGCAgtg    >  1:838015/1‑150 (MQ=255)
                                                                                                                                                  cAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCGAGCGTGAGATTTTTGGTTTCCGGAAGGAGCGAGAAAGGAAGGCCCACACCCGCAATGTTCAGCGCCgtgtt  >  2:443989/1‑149 (MQ=255)
                                                                                                                                                  cAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACCTTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCCCAACCCGAATGTTCAGCGCAGTGTa  >  2:637897/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TTCCCAGCTCATTCCTCCCAGCAAACCAATTGTTTTCATTCCTCTCTCCTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCCGGAATGAGCCAGAAAGTAATGCCCACAAACGCAATGTTCAGCGCAGTGTA  >  NC_000913/2980597‑2980892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: