Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,988,137 T→A 5.7% L201* (TTA→TAA)  yqeH → putative LuxR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,988,1370TA 5.7% 245.4 / 2.7 93L201* (TTA→TAA) yqeHputative LuxR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (39/48);  new base A (2/4);  total (41/52)
Fisher's exact test for biased strand distribution p-value = 6.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

Only 100 of 101 total aligned reads displayed.

ATTAACTCAGATGGAATTCACCATAATGGGGTATATGGTTTCTGGTTATAATCCATATCAGATTGCTGAAGTTCTGGATATGGATATCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACAC  >  NC_000913/2987988‑2988286
                                                                                                                                                     |                                                                                                                                                     
aTTAACTCAGATGGAATTCACCATAATGGGGTATATGGTTTCTGGTTATAATCCATATCAGATTGCTGAAGTTCTTGATATGGATATCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAATAGAATGGGTGGTAAAATAAACGAatt                                                                                                                                                       >  2:823286/1‑150 (MQ=255)
    aCTCAGATGGAATTCACCATAATGGGGTATATGGTTTCTGGTTATAATCCATATCAGATTGCTGAAGTTCGGGATATGGATATCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAAttattt                                                                                                                                                   <  2:357146/150‑1 (MQ=255)
       cAGATGGAATTCACCATAATGGGGTATATGGTTTCTGGTTATAATCCATATCAGATTGCTGAAGTTCTGGATATGGATATCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATtatttatt                                                                                                                                                <  2:1256820/150‑1 (MQ=255)
           tGGAATTCACCATAATGGGGTATATGGTTTCTGGTTATAATCCATATCAGATTGCTGAAGTTCTGGATATGGATATCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTAGtt                                                                                                                                            >  2:494924/1‑150 (MQ=255)
            ggAATTCACCATAATGGTGTATATGGTTTCTGGTTATAATCCATATCAGATTGCTGAAGTTCTGGATATGGATATCCGTAGCTTCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTc                                                                                                                                           <  1:295970/150‑1 (MQ=255)
            ggAATTCACCATAATGGGGTATATGGTTTCTGGTTATAATCCATATCAGATTGCTGAAGTTCTGGATATGGATATCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTc                                                                                                                                           >  1:380165/1‑150 (MQ=255)
            ggAATTCACCATAATGGGGTATATGGTTTCTGGTTATAATCCATATCAGATTGCTGAAGTTCTGGATATGGATATCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATAAATAATTCGttt                                                                                                                                           <  1:1253689/150‑2 (MQ=255)
              aaTTCACCATAATGGGGTATATGGTTTCTGGTTATAATCCATATCAGATTGCTGAAGTTCTGGATATGGATATCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTcac                                                                                                                                         <  1:1154937/150‑1 (MQ=255)
               aTTCACCATAATGGGGTATATGGTTTCTGGTTATAATCCATATCATATTGCTGAAGTTCTGGATATGGATATCCGTAGAATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATAAATAATTCGtttatt                                                                                                                                        <  1:642727/150‑5 (MQ=255)
               aTTCACCATAATGGGGTATATGGTTTCTGGTTATAATCCATATCAGATTGCTGAAGTTCTGGATATGGATATCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCTTTcaca                                                                                                                                        >  2:178669/1‑150 (MQ=255)
                ttCACCATAATGGGGTATATGGTTTCTGGTTATAATCCATATCAGATTGCTGAAGTTCTGGATATGGATATCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACAt                                                                                                                                       <  1:1185771/150‑1 (MQ=255)
                 tCACCATAATGGGGTATATGGTTTCTGGTTATAATCCATATCAGATTGCTGAAGTTCTGGATATGGATATCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATAAATAATTCGtttatttt                                                                                                                                      <  2:884300/150‑7 (MQ=255)
                  cACCATAATGGTGGATTTGGTTTCTGGTTTTATTCCTTCTCCGATAGCTGAAGTTCTGTATATTTATATCCGTATCATCTACGCGTACAAGCATCGAATAGAAAAGAGAATGGGTGGGAAAATAAACGAATTATTTATTCGTTCACATTc                                                                                                                                     <  1:84850/150‑1 (MQ=255)
                  cACCATAATGGGGTATATGGTTTCTGGTTATAATCCATATCAGATTGCTGAAGTTCTGGATATGGATATCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCTTTCACATTc                                                                                                                                     >  2:188566/1‑150 (MQ=255)
                   aCCATAATGGGGTATATGGTTTCTGGTTATAATCCATATCAGATTGCTGAAGTTCTGGATATGGATATCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTTGTAAAATAAACGAATTATTTATTCGTTCACATTCg                                                                                                                                    >  2:913046/1‑150 (MQ=255)
                   aCCATAATGGGGTATATGGTTTCTGGTTATAATCCATATCAGATTGCTGAAGTTCTGGATATGGATATCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAATTAAACGAATTATTTATTCGTTCACATTCg                                                                                                                                    >  1:73184/1‑150 (MQ=255)
                       tAATGGGGTATATGGTTTCTGGTTATAATCCATATCAGATTGCTGTAGTTCTGGATATGGATATCAGTAGCATCTACGCGTACAAGCAACGAATCGATAAGAGAATGGGAGGTAAAATAAAAGAAATATTTATACGTTCACATTCGGTcc                                                                                                                                >  2:506550/1‑150 (MQ=255)
                       tAATGGGGTATATGGTTTCTGGTTATAATCCATATCAGATTGCTGAAGTTCTGGATATGGATATCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTTGTAAAATAAACGAATTATTTATTCGTTCACATTCGGtag                                                                                                                                <  1:251930/150‑3 (MQ=255)
                          tGGGGTATATGGTTTCTGGTTATAATCCATATCAGATTGCTGAAGTTCTGGATATGGATATCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAAc                                                                                                                             <  1:501710/150‑1 (MQ=255)
                                atatTGTTTCTGGTTATAATCCATATCAGAATGCTGAAGTTCTGGATATGGATATCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGAt                                                                                                                       <  1:226440/150‑1 (MQ=255)
                                 tatGGTTTCTGGTTATAATCCATATCAGATTGCTGAAGTTCTGGATATGGATATCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATa                                                                                                                      <  1:753862/150‑1 (MQ=255)
                                  atGGTTTCTTGTTATAATCCATATCAGATTGCTGAAGTTCTGGATATGGATATCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATAc                                                                                                                     <  1:787172/150‑1 (MQ=255)
                                   tGGTTTCTGGTTATAATCCATATCAGATTGCTGAAGTTCTGGATATGGATATCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACt                                                                                                                    >  1:497347/1‑150 (MQ=255)
                                         cTGGTTATAATCCATATCAGATTGCTGAAGTTCTTGATATGGATGTCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTTGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGa                                                                                                              <  1:1103744/150‑1 (MQ=255)
                                           ggTTATAATCCATATCAGATTGCTGAAGTTCTGGATATGGATATCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGaac                                                                                                            >  1:696250/1‑148 (MQ=255)
                                           ggTTATAATCCATATCAGATTGCTGAAGTTCTGGATATGGATATCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGAcc                                                                                                            >  1:509241/1‑150 (MQ=255)
                                            gTTATAATCCATATCAGATTGCTGAAGTTCTGGATATGGATATCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGGGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGAcca                                                                                                           <  2:1016376/150‑1 (MQ=255)
                                              tataATCCATATCAGATTGCTGAAGTTCTGGATATGGATATCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGAccagc                                                                                                         <  2:440177/150‑1 (MQ=255)
                                               ataATCCATATCAGATTGCTGAAGTTCTGGATATGGATATCCGTAGCATCTACGCGTACAATCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGAccagcc                                                                                                        >  1:839896/1‑150 (MQ=255)
                                               ataATCCATATCAGATTGCTGAAGTTCTGGATATGGATATCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGAccagcc                                                                                                        >  2:399149/1‑150 (MQ=255)
                                                 aaTCCATATCAGATTGCTGAAGTTCTGGATATGGATATCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGAccagccag                                                                                                      >  1:821058/1‑150 (MQ=255)
                                                  aTCCATATCAGATTGCTGAAGTTCTGGATATGGATATCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGa                                                                                                     >  1:959678/1‑150 (MQ=255)
                                                    ccATATCAGATTGCTGAAGTTCTGGATATGGATATCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAAt                                                                                                   >  2:27102/1‑150 (MQ=255)
                                                     cATATCAGATTGCTGAAGTTCTGGATATGGATATCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCTCATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATc                                                                                                  >  1:421850/1‑150 (MQ=255)
                                                       tatCAGATTGCTGAAGTTCTGGATATGGATATCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCAt                                                                                                <  2:1266471/150‑1 (MQ=255)
                                                           aGATTGCTGAATTTCTGGATATGGATATCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATActc                                                                                            <  1:313452/150‑1 (MQ=255)
                                                              ttGCTGAAGTTCTGGATATGGATATCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAACCGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGt                                                                                         >  2:573123/1‑150 (MQ=255)
                                                                gCTGAAGTTCTGGATATGGATATCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAACCGAATAATTTATTCGTTCACATTCGGTCCAACATTGAAACTCAACGACCAGACAGAATCTTACTCTGttt                                                                                       >  1:814918/1‑150 (MQ=255)
                                                                gCTGAAGTTCTGGATATGGATATCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCCACATTGATACTCAACGACCAGCCAGAATCATACTCTGttt                                                                                       >  2:800686/1‑150 (MQ=255)
                                                                gCTGAAGTTCTGGATATGGATATCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGttt                                                                                       <  1:760457/150‑1 (MQ=255)
                                                                 cTGAAGTTCTGGATATGGATATCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTta                                                                                      >  2:606423/1‑150 (MQ=255)
                                                                 cTGAAGTTCTGGATATGGATATCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTta                                                                                      >  1:529206/1‑150 (MQ=255)
                                                                 cTGAAGTTCTGGATATGGATATCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTta                                                                                      >  1:326327/1‑150 (MQ=255)
                                                                  tGAAGTTCTGGATATGGATATCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTtat                                                                                     >  2:1113446/1‑150 (MQ=255)
                                                                       ttCTGGATATGGATATCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACTACCAGCCAGAATCATACTCTGTTTATACggg                                                                                <  2:12773/150‑1 (MQ=255)
                                                                             atatggatatCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTa                                                                          <  1:1023440/150‑1 (MQ=255)
                                                                                tggatatCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTg                                                                       >  2:101323/1‑150 (MQ=255)
                                                                                tggatatCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTg                                                                       <  2:264360/150‑1 (MQ=255)
                                                                                   atatCCGTAGCATCTCCGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGaaa                                                                    <  2:1122253/150‑1 (MQ=255)
                                                                                      tCCGTAGCAGCTACGCGTAAAATAAACGAATCGAAAAGTGAAGGCGTGTTAAAATAAACGAATTATTTATTCGTTCACAATCGGTCCAACATTGATACTCAACGACCAGCCAGATTCATACTCTGTTTATACGGGCATTTATTGAAATGt                                                                 <  2:542696/150‑1 (MQ=255)
                                                                                       ccGTAGCAGCAACGCGTACAAGCAACGAATCGAAAAGCGAACGGGTGGTAAAATAAACGAATAATTTATTCGTTCACATTCGGTCCAACATTGATACTCACCGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTc                                                                <  2:168825/150‑1 (MQ=255)
                                                                                        cGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTcc                                                               >  1:2169/1‑150 (MQ=255)
                                                                                         gTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCg                                                              >  2:1104704/1‑150 (MQ=255)
                                                                                         gTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCg                                                              >  2:1269864/1‑150 (MQ=255)
                                                                                            gCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATCTCCGTAt                                                           >  2:324577/1‑150 (MQ=255)
                                                                                              aTCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTAttt                                                         >  1:399362/1‑150 (MQ=255)
                                                                                                cTACGCGTAGAAGCAACGAATCGAAAATTGAATGGGTGTTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGACATTTCAATAAATGCCCGTAtaaac                                                       <  1:1058191/150‑5 (MQ=255)
                                                                                                cTACGCGTACAAGCTACGTATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGACATTTCAATAAATGCCCGTAtaaac                                                       <  2:1295601/150‑5 (MQ=255)
                                                                                                cTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTta                                                       >  2:25750/1‑150 (MQ=255)
                                                                                                cTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTta                                                       <  1:1283768/150‑1 (MQ=255)
                                                                                                  aCGAGTACACCCAACGAATCGAACAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGGCCAACATTGATACTCAACGACCAGCCATAATCATAATCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTtata                                                     <  2:769238/150‑1 (MQ=255)
                                                                                                    gcgTACAAGCAACGAATCGAAAAGAGAATGGGTTGTAAAATAAACGAATTATTTTTTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATaaa                                                   <  1:455079/150‑1 (MQ=255)
                                                                                                        aCAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAgg                                               <  2:264802/150‑1 (MQ=255)
                                                                                                         cAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGc                                              <  1:442481/150‑1 (MQ=255)
                                                                                                         aaaGCATCGAATCGATAAGAGAATGGGTTGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATTCTCTGTTTATACGGTCATTTATTGAAATGTCCGTATTTTATAAATAGGc                                              <  2:633125/149‑1 (MQ=255)
                                                                                                         aaaGAAACGAATATAAAATATAATTGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGc                                              <  1:840656/149‑1 (MQ=255)
                                                                                                           aGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCg                                            <  1:579962/150‑1 (MQ=255)
                                                                                                             cAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTc                                          <  2:508404/150‑1 (MQ=255)
                                                                                                             cAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAAACATACTCTCTTTATACGGGAATTTATAGAAATGTCAGTATTTTATAAATAGGCCGTc                                          >  1:133517/1‑150 (MQ=255)
                                                                                                              aaCGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCATCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTcc                                         <  2:315683/150‑1 (MQ=255)
                                                                                                              aaCGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGTGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTcc                                         <  2:1229442/150‑1 (MQ=255)
                                                                                                                cGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTcccc                                       <  2:937294/150‑1 (MQ=255)
                                                                                                                 gAATCGAACAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATAGAAATGTCCGTATTTTATAAATAGGCCGTccccg                                      <  1:99568/150‑2 (MQ=255)
                                                                                                                   aTCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAg                                                >  2:253123/1‑138 (MQ=255)
                                                                                                                   aTCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAg                                                <  1:253123/138‑1 (MQ=255)
                                                                                                                     cGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGTAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAag                                  <  2:1219578/150‑1 (MQ=255)
                                                                                                                     cGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAag                                  <  2:848271/150‑1 (MQ=255)
                                                                                                                     cGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAag                                  <  2:821058/150‑1 (MQ=255)
                                                                                                                      gAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAaga                                 >  2:1010149/1‑150 (MQ=255)
                                                                                                                          agagAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTaa                             <  1:992629/150‑1 (MQ=255)
                                                                                                                          agagAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTaa                             <  1:677859/150‑1 (MQ=255)
                                                                                                                            tgaATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGTCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAac                           <  2:1040239/149‑1 (MQ=255)
                                                                                                                                  ggtggtAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATa                     >  1:636492/1‑150 (MQ=255)
                                                                                                                                   gtgtgaaaaTAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAg                    <  2:1146035/145‑1 (MQ=255)
                                                                                                                                      gtAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTAt                 >  1:1075795/1‑150 (MQ=255)
                                                                                                                                       tAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAATACTATGGTGtt                <  2:510547/150‑1 (MQ=255)
                                                                                                                                       tAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCACTCAAGAGTAAACACCATAGTAtt                >  1:763250/1‑150 (MQ=255)
                                                                                                                                       tAAAATAAACGAATTATTTATTAGTTCACATTCGGTCCAACATTGATAATCAACGACCATCCAGAATCATACTCTGATTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTAtt                <  2:543742/150‑1 (MQ=255)
                                                                                                                                          tgtgaaCGAATAAATAATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTaa             >  1:40288/5‑150 (MQ=255)
                                                                                                                                             aaaCGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAAc          <  2:359432/150‑1 (MQ=255)
                                                                                                                                              aaCGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAAcc         <  1:926596/150‑1 (MQ=255)
                                                                                                                                              aaCGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAAcc         >  1:959213/1‑150 (MQ=255)
                                                                                                                                              aaCGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAAcc         >  2:1214620/1‑150 (MQ=255)
                                                                                                                                                 gAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCACCGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATc      >  1:86504/1‑150 (MQ=255)
                                                                                                                                                  aaTTATTTATTCGTTCACATTCGGTCCAACATTGATACTGAACGACGAGCCAGAGTCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATca     <  2:561593/150‑1 (MQ=255)
                                                                                                                                                   attattTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATcac    <  2:1011627/150‑1 (MQ=255)
                                                                                                                                                   attattTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATcac    <  1:1287771/150‑1 (MQ=255)
                                                                                                                                                     tatttattCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCacac  >  2:769071/1‑150 (MQ=255)
                                                                                                                                                     tatttattCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCacac  >  1:609565/1‑150 (MQ=255)
                                                                                                                                                     tatttattCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGAATTTATTGAAATGTCCGTATTTTATAAAAAGGACGTCCCCTCAAGAGTATACAGCATAGTATTTAAAACCATCacac  >  2:893049/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATTAACTCAGATGGAATTCACCATAATGGGGTATATGGTTTCTGGTTATAATCCATATCAGATTGCTGAAGTTCTGGATATGGATATCCGTAGCATCTACGCGTACAAGCAACGAATCGAAAAGAGAATGGGTGGTAAAATAAACGAATTATTTATTCGTTCACATTCGGTCCAACATTGATACTCAACGACCAGCCAGAATCATACTCTGTTTATACGGGCATTTATTGAAATGTCCGTATTTTATAAATAGGCCGTCCCCTCAAGAGTAAACACCATAGTATTTAAAACCATCACAC  >  NC_000913/2987988‑2988286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: