BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	312,303	T→G	6.3%	V64V (GTT→GTG)	ykgL →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	312,303	0	T	G	6.3%	277.5 / 7.7	96	V64V (GTT→GTG)	ykgL	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base T (52/38); new base G (3/3); total (55/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.91e-01

Only 100 of 102 total aligned reads displayed.

CAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCGC  >  NC_000913/312154‑312452

cAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATAAATCCCCAGGAAAATTAATGTTGGTGtt                                                                                                                                                       >  2:821120/1‑150 (MQ=255)

 aGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTta                                                                                                                                                      <  1:388843/150‑1 (MQ=255)

  gTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTtat                                                                                                                                                     >  1:1228978/1‑150 (MQ=255)

   tCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTtata                                                                                                                                                    <  2:1301513/150‑1 (MQ=255)

     tAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATaaa                                                                                                                                                  >  1:666873/1‑150 (MQ=255)

      aaaaGAGGGTTGATTCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGATTATTCTGAGAGAAACGTCATGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAATATTAATGTTGGTGTTATAAac                                                                                                                                                 <  1:213731/150‑1 (MQ=255)

          gagGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAacacac                                                                                                                                             <  1:579527/150‑1 (MQ=255)

            gggTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCa                                                                                                                                           >  2:385654/1‑150 (MQ=255)

            gggTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCa                                                                                                                                           <  2:946219/150‑1 (MQ=255)

             ggTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCaa                                                                                                                                          >  2:83538/1‑150 (MQ=255)

                 gATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTGTTTATAACACCAACAtt                                                                                                                                      <  2:114111/150‑1 (MQ=255)

                  aTGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTGTTTATAACACCAACATTa                                                                                                                                     <  1:135031/150‑1 (MQ=255)

                    gCCAGCATACAGGGGCGAAGCTGGGCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTGTTTATAACACCAACATTAAt                                                                                                                                   <  2:780507/150‑1 (MQ=255)

                        gCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGa                                                                                                                               <  1:751674/150‑1 (MQ=255)

                          aTACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATc                                                                                                                             <  2:153977/150‑1 (MQ=255)

                           tACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCt                                                                                                                            >  2:1263649/1‑150 (MQ=255)

                             cAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATAAATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGAACTGa                                                                                                                          >  1:122971/1‑150 (MQ=255)

                               ggggCGAAGCTGGTCAACAAGTCAACATTAAGATAATGCAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAAACCACCAACATTAATCCGATCTGatt                                                                                                                        >  2:365457/1‑150 (MQ=255)

                                gggCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGatta                                                                                                                       <  2:614934/150‑1 (MQ=255)

                                gggCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGatta                                                                                                                       <  2:864865/150‑1 (MQ=255)

                                 ggCGAAGCTGGTCAACAAGGCAACATTAAGATAATGGAATATTCGGAGAGACACGTCAGGCAGTTGGCCTCAAATTAACAGGAAGAATATAACCCCATGAAAATTAATGTTGGTGGTATAAACACACCAACATTAATCCGAGCTGattat                                                                                                                      <  1:184770/150‑1 (MQ=255)

                                   cGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGAttatta                                                                                                                    <  1:605096/150‑1 (MQ=255)

                                      aGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGTAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCACATGAAAATTAATGTTTGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATc                                                                                                                 <  2:685021/150‑1 (MQ=255)

                                      aGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAACATTAATGTTGGTGTTATTAACCCACCATCATGAGTCCGATGTGATGATTAATc                                                                                                                 >  2:1146953/1‑150 (MQ=255)

                                      aGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTTATTATTAAta                                                                                                                 >  2:637085/1‑149 (MQ=255)

                                      aGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATc                                                                                                                 <  1:133471/150‑1 (MQ=255)

                                       gCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCa                                                                                                                >  2:403914/1‑150 (MQ=255)

                                          ggTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGAc                                                                                                             >  1:641055/1‑150 (MQ=255)

                                          ggTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGGTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATACGATCTGATTATTAATCataa                                                                                                             >  1:287877/1‑147 (MQ=255)

                                          ggTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGACAGGCAGTTGGCCTCAAATGAACAGGAAGAAAATAACCCCAGGAAAATTAATGTTGGTGTTAAAAACACACCAACATTAATACGATATGATTATTAATCAGaa                                                                                                             >  1:980098/1‑149 (MQ=255)

                                           gTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAAAAGGAAGAATATATGCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAAACCGATCTGATTATTAATAAGACa                                                                                                            >  2:592498/1‑150 (MQ=255)

                                             caacaaGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGTCATTTTGCCTCAAATGCTCAGGAAGAATATATCACCAGGAAAATTAATGTTTTTGTTATAAACACACCAACATTAATCCGATCTGATTAATAATCAGatcgg                                                                                                          <  2:196326/150‑5 (MQ=255)

                                             caacaaGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATAAGAaaaa                                                                                                          >  1:345767/1‑150 (MQ=255)

                                                   gTCAACATTAAGATAATGGAATATTCGGCGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCTCCAGGAAAATTAATGTTGGTGTTATAAAAACACCAACATTAATACGATCTGATTATTAATCAGACAAATATCGt                                                                                                    >  1:368472/1‑150 (MQ=255)

                                                   gTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGt                                                                                                    >  1:287099/1‑150 (MQ=255)

                                                     cAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTa                                                                                                  >  1:878178/1‑150 (MQ=255)

                                                      aaCATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTaa                                                                                                 <  2:723873/150‑1 (MQ=255)

                                                        cATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACGCCAACATTAATACGATCTGATTATTAATCAGACAAATATCGTTAATc                                                                                               >  2:1209979/1‑150 (MQ=255)

                                                          ttAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCg                                                                                             <  2:499457/150‑1 (MQ=255)

                                                          ttAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCg                                                                                             >  1:430288/1‑150 (MQ=255)

                                                             aGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCg                                                                                          >  1:468510/1‑150 (MQ=255)

                                                                tAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACCCCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAAt                                                                                       >  2:502666/1‑150 (MQ=255)

                                                                   tGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAAtttt                                                                                    >  2:351134/1‑150 (MQ=255)

                                                                    ggAATATTCGGAGAGAACCGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATGATTAATCAGACAAATATCGTTAATCCGGCGAATTTTg                                                                                   >  1:1293136/1‑150 (MQ=255)

                                                                    ggAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATTTATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTg                                                                                   >  2:435162/1‑150 (MQ=255)

                                                                       atatTCGGAGAGAACCGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAAt                                                                                <  2:584857/150‑1 (MQ=255)

                                                                        tatTCGGAGAGAAAGGTCAGGCAGTTGGCCTGAAATGAACAGGAAGAATATATTCCCAGGAAATTTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGTCAAATATCGTTAATCCGTCGAATTTTGAATc                                                                               <  2:596834/150‑1 (MQ=255)

                                                                        tatTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATc                                                                               >  2:1281783/1‑150 (MQ=255)

                                                                            cGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACg                                                                           <  2:266967/150‑1 (MQ=255)

                                                                            cGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACg                                                                           >  1:119504/1‑150 (MQ=255)

                                                                             ggAGAGAAAAGTCACGCAGTTGGCCTAAAATGATCAAGAAGAATTTATAACAAGGAAAATTAATGTTGGTGTTTTAAAAACAAAAAAAATAATCAGATATGATTTATAATCATACAAAAAAATGTAAACCGTCATAGTTTGAATCAACGt                                                                          >  2:1187972/1‑150 (MQ=255)

                                                                                 agaAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCACAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGttttt                                                                      <  1:83538/150‑1 (MQ=255)

                                                                                  gaAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGtttttt                                                                     >  2:423716/1‑150 (MQ=255)

                                                                                  gaAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGtttttt                                                                     >  1:529345/1‑150 (MQ=255)

                                                                                    aaCGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCt                                                                   >  1:215540/1‑150 (MQ=255)

                                                                                    aaCGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCt                                                                   <  1:1263649/150‑1 (MQ=255)

                                                                                      cGTCAGGCAGGTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTa                                                                 <  1:1092096/150‑1 (MQ=255)

                                                                                       gTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGATAATCCGTCGAATTTTGAATAAAATTTTTTTCTTAc                                                                >  1:90879/1‑150 (MQ=255)

                                                                                            gCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACTAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGAccc                                                           <  2:125674/150‑1 (MQ=255)

                                                                                                 tGGCCTCAAATGAAAATTAAGAATATTTCCCAAGGTAAATTAATTGTGGTGATATAAACACAGCAACATTAATCCGATCTGATTATTAATCAGACTAATATCTTTAATCCGGCTAATTTTGAATCAACGTTTTTTCTTACGACCCTGAAc                                                      <  1:190479/150‑1 (MQ=255)

                                                                                                   gCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACgg                                                    <  2:311024/150‑1 (MQ=255)

                                                                                                    ccTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGc                                                   >  2:396958/1‑150 (MQ=255)

                                                                                                     cTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGcc                                                  >  2:746511/1‑150 (MQ=255)

                                                                                                      tCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCTTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCt                                                 <  2:510691/150‑1 (MQ=255)

                                                                                                      tCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCt                                                 >  1:1292162/1‑150 (MQ=255)

                                                                                                      tCAAATGAACAGGAACAATATATCCACAGGAAAATTAAATTTGGTGTTTTAAACACACCAACATTAATCCGATCTGATTAATAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCt                                                 <  2:121050/150‑1 (MQ=255)

                                                                                                       cAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCtt                                                >  2:1161702/1‑150 (MQ=255)

                                                                                                       cAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATACGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCtt                                                <  2:1136635/150‑1 (MQ=255)

                                                                                                       aaaaTGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCtt                                                <  1:704919/149‑1 (MQ=255)

                                                                                                        aaaTGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTa                                               <  2:716642/150‑1 (MQ=255)

                                                                                                         attGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACTACCCTGAACTGCCTTaa                                              >  2:567629/3‑150 (MQ=255)

                                                                                                           tGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTaaaa                                            >  2:303598/1‑150 (MQ=255)

                                                                                                             aaCAGGAAGAATATATCCACAGGAAAATTAATGTTGGTGTTATAAACACACAAAGATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTGTTTTCTTACGACCCTGAACGGCCTTAAAACg                                          <  1:646342/150‑1 (MQ=255)

                                                                                                               cAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCATTAAAACGTg                                        >  2:116429/1‑150 (MQ=255)

                                                                                                                aGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTgg                                       >  1:1056646/1‑150 (MQ=255)

                                                                                                                 ggAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTTATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGa                                      >  2:446883/1‑150 (MQ=255)

                                                                                                                 ggAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGa                                      <  1:403914/150‑1 (MQ=255)

                                                                                                                  gaagaaTATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGAAAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTAAGACACTGAACGGCATTAAAACGTGGAt                                     >  1:160951/1‑150 (MQ=255)

                                                                                                                   aagaaTATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGAtt                                    <  1:1161702/150‑1 (MQ=255)

                                                                                                                                cAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATc                       >  1:1058741/1‑150 (MQ=255)

                                                                                                                                  ggAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATcac                     <  1:1260661/150‑1 (MQ=255)

                                                                                                                                   gAAAATTAATGTTGGTGTTATAAACACACCATCATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATcaca                    <  1:351134/150‑1 (MQ=255)

                                                                                                                                   gAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATcaca                    <  2:929705/150‑1 (MQ=255)

                                                                                                                                    aaaaTTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACat                   >  1:50428/1‑150 (MQ=255)

                                                                                                                                     aaaTTAATGTTGGTGTTATAAACACACCAACCTTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGCCCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACata                  >  1:940817/1‑150 (MQ=255)

                                                                                                                                      gaTTAATGTTGGTGTGTTTATAACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACataa                 >  2:386214/2‑149 (MQ=39)

                                                                                                                                      aaTTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACatat                 <  1:746511/150‑1 (MQ=255)

                                                                                                                                         tAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAACACGGGGATTAGATTTACAAATCACATATAGc              <  1:562625/150‑1 (MQ=255)

                                                                                                                                         tAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGc              <  2:902847/150‑1 (MQ=255)

                                                                                                                                          aaTGTTGGTGTTATAAACACACCAACATTAATCCGATGTGATTATTAATCAGACAAATATCGTTAATCCGTCGTATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAATACGTGGATTAGATATACAAATCACATATAGcc             >  2:643720/1‑150 (MQ=255)

                                                                                                                                          aaTGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGcc             >  2:643718/1‑150 (MQ=255)

                                                                                                                                           aTGTTGGTGTGTTTATAACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCg            >  1:1024215/1‑150 (MQ=255)

                                                                                                                                            tgttgttgtTATAAACACAACAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGt           <  1:231280/150‑1 (MQ=255)

                                                                                                                                            tGTTGGTGTGTTTATAACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGt           >  1:1125898/1‑150 (MQ=255)

                                                                                                                                                  tgtTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTAAATCCGTCGAATATTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTcggttt     >  1:727917/1‑145 (MQ=255)

                                                                                                                                                   gtTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTc    >  1:544476/1‑150 (MQ=255)

                                                                                                                                                   gtTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTc    >  2:1241616/1‑150 (MQ=255)

                                                                                                                                                   gtTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTc    >  2:1250453/1‑150 (MQ=255)

                                                                                                                                                    ttATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCg   <  1:1027702/150‑1 (MQ=255)

                                                                                                                                                     tataAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCGc  >  1:451866/1‑150 (MQ=255)

CAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCGC  >  NC_000913/312154‑312452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`