Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,033,628 T→A 7.0% intergenic (+15/+29) idi → / ← lysS isopentenyl diphosphate isomerase/lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,033,6280TA 7.0% 212.0 / 4.7 80intergenic (+15/+29)idi/lysSisopentenyl diphosphate isomerase/lysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base T (30/44);  new base A (4/2);  total (34/46)
Fisher's exact test for biased strand distribution p-value = 3.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

TATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGT  >  NC_000913/3033479‑3033777
                                                                                                                                                     |                                                                                                                                                     
tATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACAtt                                                                                                                                                       >  1:465349/1‑150 (MQ=255)
    aaTGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGcc                                                                                                                                                   <  1:669189/150‑1 (MQ=255)
         tgtgATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGt                                                                                                                                              >  1:220763/1‑150 (MQ=255)
         tgtgATTTAGCAGATGTATTACACGGAATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCTACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGt                                                                                                                                              <  2:1182136/150‑1 (MQ=255)
         tgtgATTTACCAGATGTATTACACGGAATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGt                                                                                                                                              <  1:504361/150‑1 (MQ=255)
           tgATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTTTCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCAGGGGtgg                                                                                                                                            >  1:536059/1‑148 (MQ=255)
              tttAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGGCAAATGTCGGGGtttttt                                                                                                                                         <  1:10759/150‑5 (MQ=255)
              tttAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGGCAAATGTCGGGGtttttt                                                                                                                                         <  2:320228/150‑5 (MQ=255)
                    aGATTTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTTATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCAGCATTTACCCAGCATAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATaa                                                                                                                                   <  1:1232289/150‑1 (MQ=255)
                    aGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATaa                                                                                                                                   <  1:543153/150‑1 (MQ=255)
                     gATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAAc                                                                                                                                  >  1:1021289/1‑150 (MQ=255)
                       ggTAATACACGGTATTGAAGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCCGGCGACAAATCGCGAAGCCAGACAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGt                                                                                                                                <  1:174443/149‑1 (MQ=255)
                         tATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTaa                                                                                                                              <  1:1278285/150‑1 (MQ=255)
                           ttACACGGTATGGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAAACGCGAAGCCAGAAAACGATTCTCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATg                                                                                                                            <  1:101574/150‑1 (MQ=255)
                            tccacGGAATTGATGCCACGCCGTGGGCGTTCTGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGCTTATCTGCATTAACCCAGCTTAAATAAAAAAACCCCGACAATTGCCGGGGTTGTGAGCATAACGTAATGc                                                                                                                           <  1:784678/148‑1 (MQ=255)
                             acacGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCt                                                                                                                          >  2:609797/1‑150 (MQ=255)
                             acacGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCt                                                                                                                          >  2:1048029/1‑150 (MQ=255)
                             acacGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCt                                                                                                                          >  2:927829/1‑150 (MQ=255)
                                   tATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTAtttt                                                                                                                    <  1:1100731/150‑1 (MQ=255)
                                   tATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGAGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTAtttt                                                                                                                    <  2:476753/150‑1 (MQ=255)
                                    aTTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTa                                                                                                                   <  1:473222/150‑1 (MQ=255)
                                        aTGCCACGCCGTTGGCGTTCAGTCCGTGGATGGTTATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCgg                                                                                                               <  1:1002949/150‑1 (MQ=255)
                                             aCGTCGTTGGCGTTCAGTCCGTGGTTGGTGATGCAGGCGACAAATCGCGAAGCCAGAACACGAATATCTGCATTTTCCCAGCTTAAATAAAAAAACCCCGCCATTTGCCGGGGATGTGAGCATAACGTAATGCTTATTTTACCGGACGCa                                                                                                          <  2:191409/150‑1 (MQ=255)
                                              cGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCAt                                                                                                         >  1:774715/1‑150 (MQ=255)
                                                ccGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCg                                                                                                       >  1:1289710/1‑150 (MQ=255)
                                                     ggCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCggg                                                                                                  <  1:866223/150‑1 (MQ=255)
                                                     ggCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCggg                                                                                                  <  2:405570/150‑1 (MQ=255)
                                                     ggCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCggg                                                                                                  <  1:1203264/150‑1 (MQ=255)
                                                     ggCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACAATTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCggg                                                                                                  <  1:634762/150‑1 (MQ=255)
                                                      gCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGa                                                                                                 >  2:950299/1‑150 (MQ=255)
                                                             gTCCGTTGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAAt                                                                                          <  1:190706/150‑1 (MQ=255)
                                                               ccGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATaa                                                                                        >  2:1147524/1‑150 (MQ=255)
                                                                    gATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTcg                                                                                   <  2:793121/150‑1 (MQ=255)
                                                                     aTGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTcgc                                                                                  <  2:476599/150‑1 (MQ=255)
                                                                        gTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGa                                                                               >  1:1154271/1‑150 (MQ=255)
                                                                        gTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACAGTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGa                                                                               >  1:361608/1‑150 (MQ=255)
                                                                           aTGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg                                                                            >  1:796868/1‑150 (MQ=255)
                                                                             gCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTa                                                                          <  1:1289330/150‑1 (MQ=255)
                                                                                ggCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATgg                                                                       <  2:921452/150‑1 (MQ=255)
                                                                                 gCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGc                                                                      >  2:977057/1‑150 (MQ=255)
                                                                                 gCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGc                                                                      <  1:513992/150‑1 (MQ=255)
                                                                                      aaaTCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTg                                                                 <  1:54720/150‑1 (MQ=255)
                                                                                        aTCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGt                                                               >  1:355324/1‑150 (MQ=255)
                                                                                          cgcgAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGa                                                             <  1:764977/150‑1 (MQ=255)
                                                                                          cgcgAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGa                                                             <  1:1214591/150‑1 (MQ=255)
                                                                                           gcgAAGCCAGAAAACGATTATCTGCATTTACCCGGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGATGTTGGTGaa                                                            >  1:445111/1‑150 (MQ=255)
                                                                                            cgAAGCCAGAAAACGATTATCTGCAATAACCCAGATTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATAGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAAc                                                           >  1:814273/1‑150 (MQ=255)
                                                                                               aGCCATAATCCGATTATCTGCATTTACCCAGCTTAAATAAAAACACCCCGACATTTGCCGGGGTTGTGAGCATCCCGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGc                                                        <  1:218733/150‑1 (MQ=255)
                                                                                                  cAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCAtt                                                     <  1:910572/150‑1 (MQ=255)
                                                                                                  cAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCAtt                                                     >  2:802042/1‑150 (MQ=255)
                                                                                                  cAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCAtt                                                     >  2:1101279/1‑150 (MQ=255)
                                                                                                    taaaaCGTTTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTAc                                                   <  1:985803/149‑1 (MQ=255)
                                                                                                        aCGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTAATGCTGTTCACCAACAGCCATACCAtc                                               <  2:348602/150‑2 (MQ=255)
                                                                                                             tATCTGCATTTACCCAGCTTAAATAAATAAACCCCGACATTTGCCGGTGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGTAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAAGAGCATTACCATACGGTc                                          <  1:1065544/150‑1 (MQ=255)
                                                                                                                cTGCATTTACCCAGCTTAACTAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGAt                                       <  1:1241003/150‑1 (MQ=255)
                                                                                                                  gCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATAc                                     <  2:1289710/150‑1 (MQ=255)
                                                                                                                       tACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAAtt                                >  1:142677/1‑150 (MQ=255)
                                                                                                                          ccAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTccc                             <  2:1255134/150‑1 (MQ=255)
                                                                                                                           cAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCAGGGAACAGAAAAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCa                            >  2:351805/1‑150 (MQ=255)
                                                                                                                              cTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGAc                         <  2:425804/150‑1 (MQ=255)
                                                                                                                               ttAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGAcc                        >  2:1030301/1‑150 (MQ=255)
                                                                                                                               ttAAATAAAAAAAACCCGAAATTTGCCTGGGTTGTGAGCATAACTTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATTGTATGGCTGTTGGTGAACATCATTAACATACGTTCGATACCAATTCCCAGAca                        <  2:508591/150‑2 (MQ=255)
                                                                                                                                taaataaaAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTTATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACct                       >  1:763063/1‑150 (MQ=255)
                                                                                                                                  aataaaAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctgc                     <  1:1238017/150‑1 (MQ=255)
                                                                                                                                     ctcacaaCCCCGGCAAATGTCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGTACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGt                  >  1:973182/6‑150 (MQ=255)
                                                                                                                                     aaaaaaaCCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACAATACGGTCGATACCAATTCCCAGACCTGCTGt                  >  2:290627/1‑150 (MQ=255)
                                                                                                                                     aaaaaaaCCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGCGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctgcttt                  >  2:183691/1‑148 (MQ=255)
                                                                                                                                      tcacaaCCCCGGCAAATGTCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTc                 >  2:1062847/5‑150 (MQ=255)
                                                                                                                                       aaacaCCCCGACATTTGCCGGGGTTGTGAGCAAAACGTAATGCTTAATATACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTcg                <  2:110919/150‑1 (MQ=255)
                                                                                                                                          aaCCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATATGGGCGATACCAATTCCCAGACCTGCTGTcggcg             <  2:564141/150‑1 (MQ=255)
                                                                                                                                           aCCCCGGCAAATGTCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTcggcgg            >  2:1248110/1‑150 (MQ=255)
                                                                                                                                           aCCCCGGCAAATGTCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGATGTcggcgg            >  2:188912/1‑150 (MQ=255)
                                                                                                                                           aCCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTcggcgg            <  2:373471/150‑1 (MQ=255)
                                                                                                                                           aCCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACAGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTAAGGCTGTTGGTGAACAGCATTAACATACGGTCGCTACCAATTCACAGACCTGCTGTcggcgg            >  2:1067677/1‑150 (MQ=255)
                                                                                                                                            ccccGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGt           >  1:762230/1‑150 (MQ=255)
                                                                                                                                            ccccGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGt           <  2:566750/150‑1 (MQ=255)
                                                                                                                                            ccccGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGt           <  1:609797/150‑1 (MQ=255)
                                                                                                                                              ccGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTTCCATACGGTCGATACCAATTCCCAGCCCTGCTGTCGGCGGTaa         >  2:607447/1‑150 (MQ=255)
                                                                                                                                              ccGACATTTGCCGGGGCTGTGAGCAAAACGTAATGCTTATTTTACCGGACGCCTCGCCGGGAACAGAATAACGTCGCGGAGGGTATGGCTGGTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTaa         <  1:940551/150‑1 (MQ=255)
                                                                                                                                                   aTTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCAt    >  1:452194/1‑150 (MQ=255)
                                                                                                                                                   aTTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCAt    >  1:587077/1‑150 (MQ=255)
                                                                                                                                                     ttGCCGGGGTTGTGAGCATAACGTAATGCTTAATTTACCGGACGCATCGGCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGt  <  2:474147/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGT  >  NC_000913/3033479‑3033777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: