Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,033,631 C→T 7.4% intergenic (+18/+26) idi → / ← lysS isopentenyl diphosphate isomerase/lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,033,6310CT 7.4% 232.5 / 6.6 81intergenic (+18/+26)idi/lysSisopentenyl diphosphate isomerase/lysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base C (30/45);  new base T (4/2);  total (34/47)
Fisher's exact test for biased strand distribution p-value = 2.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.72e-01

AATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCC  >  NC_000913/3033483‑3033780
                                                                                                                                                    |                                                                                                                                                     
aaTGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGcc                                                                                                                                                      <  1:669189/150‑1 (MQ=255)
     tgtgATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGt                                                                                                                                                 >  1:220763/1‑150 (MQ=255)
     tgtgATTTAGCAGATGTATTACACGGAATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCTACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGt                                                                                                                                                 <  2:1182136/150‑1 (MQ=255)
     tgtgATTTACCAGATGTATTACACGGAATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGt                                                                                                                                                 <  1:504361/150‑1 (MQ=255)
       tgATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTTTCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCAGGGGtgg                                                                                                                                               >  1:536059/1‑148 (MQ=255)
          tttAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGGCAAATGTCGGGGtttttt                                                                                                                                            <  1:10759/150‑5 (MQ=255)
          tttAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGGCAAATGTCGGGGtttttt                                                                                                                                            <  2:320228/150‑5 (MQ=255)
                aGATTTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTTATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCAGCATTTACCCAGCATAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATaa                                                                                                                                      <  1:1232289/150‑1 (MQ=255)
                aGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATaa                                                                                                                                      <  1:543153/150‑1 (MQ=255)
                 gATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAAc                                                                                                                                     >  1:1021289/1‑150 (MQ=255)
                   ggTAATACACGGTATTGAAGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCCGGCGACAAATCGCGAAGCCAGACAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGt                                                                                                                                   <  1:174443/149‑1 (MQ=255)
                     tATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTaa                                                                                                                                 <  1:1278285/150‑1 (MQ=255)
                       ttACACGGTATGGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAAACGCGAAGCCAGAAAACGATTCTCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATg                                                                                                                               <  1:101574/150‑1 (MQ=255)
                        tccacGGAATTGATGCCACGCCGTGGGCGTTCTGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGCTTATCTGCATTAACCCAGCTTAAATAAAAAAACCCCGACAATTGCCGGGGTTGTGAGCATAACGTAATGc                                                                                                                              <  1:784678/148‑1 (MQ=255)
                         acacGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCt                                                                                                                             >  2:1048029/1‑150 (MQ=255)
                         acacGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCt                                                                                                                             >  2:609797/1‑150 (MQ=255)
                         acacGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCt                                                                                                                             >  2:927829/1‑150 (MQ=255)
                               tATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTAtttt                                                                                                                       <  1:1100731/150‑1 (MQ=255)
                               tATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGAGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTAtttt                                                                                                                       <  2:476753/150‑1 (MQ=255)
                                aTTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTa                                                                                                                      <  1:473222/150‑1 (MQ=255)
                                    aTGCCACGCCGTTGGCGTTCAGTCCGTGGATGGTTATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCgg                                                                                                                  <  1:1002949/150‑1 (MQ=255)
                                         aCGTCGTTGGCGTTCAGTCCGTGGTTGGTGATGCAGGCGACAAATCGCGAAGCCAGAACACGAATATCTGCATTTTCCCAGCTTAAATAAAAAAACCCCGCCATTTGCCGGGGATGTGAGCATAACGTAATGCTTATTTTACCGGACGCa                                                                                                             <  2:191409/150‑1 (MQ=255)
                                          cGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCAt                                                                                                            >  1:774715/1‑150 (MQ=255)
                                            ccGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCg                                                                                                          >  1:1289710/1‑150 (MQ=255)
                                                 ggCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCggg                                                                                                     <  2:405570/150‑1 (MQ=255)
                                                 ggCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCggg                                                                                                     <  1:1203264/150‑1 (MQ=255)
                                                 ggCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCggg                                                                                                     <  1:866223/150‑1 (MQ=255)
                                                 ggCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACAATTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCggg                                                                                                     <  1:634762/150‑1 (MQ=255)
                                                  gCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGa                                                                                                    >  2:950299/1‑150 (MQ=255)
                                                         gTCCGTTGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAAt                                                                                             <  1:190706/150‑1 (MQ=255)
                                                           ccGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATaa                                                                                           >  2:1147524/1‑150 (MQ=255)
                                                                gATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTcg                                                                                      <  2:793121/150‑1 (MQ=255)
                                                                 aTGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTcgc                                                                                     <  2:476599/150‑1 (MQ=255)
                                                                    gTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGa                                                                                  >  1:1154271/1‑150 (MQ=255)
                                                                    gTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACAGTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGa                                                                                  >  1:361608/1‑150 (MQ=255)
                                                                       aTGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg                                                                               >  1:796868/1‑150 (MQ=255)
                                                                         gCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTa                                                                             <  1:1289330/150‑1 (MQ=255)
                                                                            ggCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATgg                                                                          <  2:921452/150‑1 (MQ=255)
                                                                             gCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGc                                                                         >  2:977057/1‑150 (MQ=255)
                                                                             gCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGc                                                                         <  1:513992/150‑1 (MQ=255)
                                                                                  aaaTCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTg                                                                    <  1:54720/150‑1 (MQ=255)
                                                                                    aTCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGt                                                                  >  1:355324/1‑150 (MQ=255)
                                                                                      cgcgAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGa                                                                <  1:764977/150‑1 (MQ=255)
                                                                                      cgcgAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGa                                                                <  1:1214591/150‑1 (MQ=255)
                                                                                       gcgAAGCCAGAAAACGATTATCTGCATTTACCCGGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGATGTTGGTGaa                                                               >  1:445111/1‑150 (MQ=255)
                                                                                        cgAAGCCAGAAAACGATTATCTGCAATAACCCAGATTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATAGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAAc                                                              >  1:814273/1‑150 (MQ=255)
                                                                                           aGCCATAATCCGATTATCTGCATTTACCCAGCTTAAATAAAAACACCCCGACATTTGCCGGGGTTGTGAGCATCCCGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGc                                                           <  1:218733/150‑1 (MQ=255)
                                                                                              cAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCAtt                                                        <  1:910572/150‑1 (MQ=255)
                                                                                              cAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCAtt                                                        >  2:802042/1‑150 (MQ=255)
                                                                                              cAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCAtt                                                        >  2:1101279/1‑150 (MQ=255)
                                                                                                taaaaCGTTTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTAc                                                      <  1:985803/149‑1 (MQ=255)
                                                                                                    aCGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTAATGCTGTTCACCAACAGCCATACCAtc                                                  <  2:348602/150‑2 (MQ=255)
                                                                                                         tATCTGCATTTACCCAGCTTAAATAAATAAACCCCGACATTTGCCGGTGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGTAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAAGAGCATTACCATACGGTc                                             <  1:1065544/150‑1 (MQ=255)
                                                                                                            cTGCATTTACCCAGCTTAACTAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGAt                                          <  1:1241003/150‑1 (MQ=255)
                                                                                                              gCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATAc                                        <  2:1289710/150‑1 (MQ=255)
                                                                                                                   tACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAAtt                                   >  1:142677/1‑150 (MQ=255)
                                                                                                                      ccAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTccc                                <  2:1255134/150‑1 (MQ=255)
                                                                                                                       cAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCAGGGAACAGAAAAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCa                               >  2:351805/1‑150 (MQ=255)
                                                                                                                          cTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGAc                            <  2:425804/150‑1 (MQ=255)
                                                                                                                           ttAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGAcc                           >  2:1030301/1‑150 (MQ=255)
                                                                                                                           ttAAATAAAAAAAACCCGAAATTTGCCTGGGTTGTGAGCATAACTTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATTGTATGGCTGTTGGTGAACATCATTAACATACGTTCGATACCAATTCCCAGAca                           <  2:508591/150‑2 (MQ=255)
                                                                                                                            taaataaaAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTTATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACct                          >  1:763063/1‑150 (MQ=255)
                                                                                                                              aataaaAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctgc                        <  1:1238017/150‑1 (MQ=255)
                                                                                                                                 ctcacaaCCCCGGCAAATGTCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGTACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGt                     >  1:973182/6‑150 (MQ=255)
                                                                                                                                 aaaaaaaCCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACAATACGGTCGATACCAATTCCCAGACCTGCTGt                     >  2:290627/1‑150 (MQ=255)
                                                                                                                                 aaaaaaaCCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGCGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctgcttt                     >  2:183691/1‑148 (MQ=255)
                                                                                                                                  tcacaaCCCCGGCAAATGTCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTc                    >  2:1062847/5‑150 (MQ=255)
                                                                                                                                   aaacaCCCCGACATTTGCCGGGGTTGTGAGCAAAACGTAATGCTTAATATACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTcg                   <  2:110919/150‑1 (MQ=255)
                                                                                                                                      aaCCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATATGGGCGATACCAATTCCCAGACCTGCTGTcggcg                <  2:564141/150‑1 (MQ=255)
                                                                                                                                       aCCCCGGCAAATGTCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTcggcgg               >  2:1248110/1‑150 (MQ=255)
                                                                                                                                       aCCCCGGCAAATGTCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGATGTcggcgg               >  2:188912/1‑150 (MQ=255)
                                                                                                                                       aCCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTcggcgg               <  2:373471/150‑1 (MQ=255)
                                                                                                                                       aCCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACAGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTAAGGCTGTTGGTGAACAGCATTAACATACGGTCGCTACCAATTCACAGACCTGCTGTcggcgg               >  2:1067677/1‑150 (MQ=255)
                                                                                                                                        ccccGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGt              <  1:609797/150‑1 (MQ=255)
                                                                                                                                        ccccGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGt              >  1:762230/1‑150 (MQ=255)
                                                                                                                                        ccccGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGt              <  2:566750/150‑1 (MQ=255)
                                                                                                                                          ccGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTTCCATACGGTCGATACCAATTCCCAGCCCTGCTGTCGGCGGTaa            >  2:607447/1‑150 (MQ=255)
                                                                                                                                          ccGACATTTGCCGGGGCTGTGAGCAAAACGTAATGCTTATTTTACCGGACGCCTCGCCGGGAACAGAATAACGTCGCGGAGGGTATGGCTGGTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTaa            <  1:940551/150‑1 (MQ=255)
                                                                                                                                               aTTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCAt       >  1:587077/1‑150 (MQ=255)
                                                                                                                                               aTTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCAt       >  1:452194/1‑150 (MQ=255)
                                                                                                                                                 ttGCCGGGGTTGTGAGCATAACGTAATGCTTAATTTACCGGACGCATCGGCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGt     <  2:474147/150‑1 (MQ=255)
                                                                                                                                                   gCCGGGGTTGTGACCAAACCGTACTGCATATTTTACCGGACGCATCGCCGGCAACAGAAAAACGTCGCGGATGGTATGGCTGTCGGCGAACAGCATTACCATACGGTCGATACCAATTCCCACACCTGCTGTCGGCGGTAAGCCATGTTc   <  2:175834/150‑1 (MQ=255)
                                                                                                                                                    ccGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTcc  >  1:621343/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCC  >  NC_000913/3033483‑3033780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: