Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,034,807 C→T 5.8% G123D (GGC→GAC)  lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,034,8070CT 5.8% 207.9 / 2.8 69G123D (GGC→GAC) lysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base C (32/33);  new base T (2/2);  total (34/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01

CGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCAT  >  NC_000913/3034658‑3034955
                                                                                                                                                     |                                                                                                                                                    
cGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCgcgc                                                                                                                                                      >  1:1229692/1‑150 (MQ=255)
    aTAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAg                                                                                                                                                  >  1:1090022/1‑150 (MQ=255)
    aTAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAg                                                                                                                                                  <  1:456088/150‑1 (MQ=255)
            gACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGc                                                                                                                                          <  2:670114/150‑1 (MQ=255)
                aTAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAg                                                                                                                                      >  2:378220/1‑150 (MQ=255)
                 aaGCGCGCTTCCGGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAgg                                                                                                                                     <  1:660958/149‑1 (MQ=255)
                          tCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACTCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCAtttt                                                                                                                            >  1:1078244/1‑150 (MQ=255)
                          tCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCAtttt                                                                                                                            >  2:562468/1‑150 (MQ=255)
                            cTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCAtttttt                                                                                                                          <  1:313645/150‑1 (MQ=255)
                             ggATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGGGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTg                                                                                                                         <  1:993744/149‑1 (MQ=255)
                               aTCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGaa                                                                                                                       <  2:8758/150‑1 (MQ=255)
                                tCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGTCGAGGATGTCGCCGAGGTCCCATTTTTTGAAc                                                                                                                      >  1:918049/1‑150 (MQ=255)
                                tCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGTCGAGGATGTCGCCGAGGTCCCATTTTTTGAAc                                                                                                                      >  1:922683/1‑150 (MQ=255)
                                tCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAAc                                                                                                                      >  2:1232260/1‑150 (MQ=255)
                                tCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTGCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAAc                                                                                                                      <  1:995655/150‑1 (MQ=255)
                                 ccTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACt                                                                                                                     <  1:890432/150‑1 (MQ=255)
                                  cTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTg                                                                                                                    >  2:509741/1‑150 (MQ=255)
                                          cGTGGAATTTAACCGGCAGCGGACGCAGTGCGGTGGTCAGCACACGCATCTCGGTGCAGTGGATAGACAGCGCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGTATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTtat                                                                                                            <  1:1011747/150‑1 (MQ=255)
                                           gTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGGTCGCCGGTTTTGGGTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTtata                                                                                                           <  2:74472/150‑1 (MQ=255)
                                                aTTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGc                                                                                                      <  2:1240317/150‑1 (MQ=255)
                                                aTTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGc                                                                                                      <  2:1223192/150‑1 (MQ=255)
                                                   tATCCTGCAGCGGCCGCAGTGCGTTGGTCAGCAGACGCCACTCGGTGCTGTGGATAGACAGTTCGCCGGTTTTGTTTTTTCACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATATTATGAACTGCTCGTTATAACCGACtt                                                                                                   >  1:208242/1‑150 (MQ=255)
                                                   tATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCtt                                                                                                   >  2:313917/1‑150 (MQ=255)
                                                          cAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTTGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGgag                                                                                            >  2:434062/1‑150 (MQ=255)
                                                          cAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGgag                                                                                            >  2:459652/1‑150 (MQ=255)
                                                                 cGCAGTGCTTTGGGCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGCCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCa                                                                                     <  1:154361/150‑1 (MQ=255)
                                                                   cAGTGCTTTGGTCAGCAGACGCAACTCGGTGCATTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGATCGTTATAAACCCCTTCCGGGAGATCGTCACg                                                                                   >  1:332757/1‑150 (MQ=255)
                                                                   cAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACg                                                                                   <  1:250869/150‑1 (MQ=255)
                                                                   cAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCACATTATTTGAACTGCTCGTAATAAACGCATTCCGGGAGATCGTCACg                                                                                   >  2:418854/1‑150 (MQ=255)
                                                                      tGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCTCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGAGCGACCCGGGc                                                                                >  1:671609/1‑150 (MQ=255)
                                                                      tGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGc                                                                                >  1:958395/1‑150 (MQ=255)
                                                                        cTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGCCAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCaa                                                                              >  1:842880/1‑150 (MQ=255)
                                                                        cTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCaa                                                                              >  1:1115130/1‑150 (MQ=255)
                                                                          ttGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACg                                                                            >  2:1210625/1‑150 (MQ=255)
                                                                                agcagACGGAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGc                                                                      <  1:1111167/150‑1 (MQ=255)
                                                                                agcagACGCAACGCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGc                                                                      <  2:904658/150‑1 (MQ=255)
                                                                                 gcagACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACTCCTTCCGGGAGATCGTCACGGGCAACGTACAGCt                                                                     >  1:995536/1‑150 (MQ=255)
                                                                                  cagACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACTCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTg                                                                    <  2:1078244/150‑1 (MQ=255)
                                                                                      cGCAAGTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATg                                                                <  2:482266/150‑1 (MQ=255)
                                                                                      cGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATg                                                                >  2:599331/1‑150 (MQ=255)
                                                                                       gCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGc                                                               >  1:903339/1‑150 (MQ=255)
                                                                                          aCTCGGTGCAGTGGATAGACAGTTCGACGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGAc                                                            <  2:1119776/150‑1 (MQ=255)
                                                                                              ggTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGcc                                                        <  2:341360/150‑1 (MQ=255)
                                                                                              ggTCCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTAGCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGATCTTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGcc                                                        <  1:1255008/150‑1 (MQ=255)
                                                                                                   aGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTTGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGt                                                   <  1:276937/150‑1 (MQ=255)
                                                                                                     gggATAGACAGATCGCCGGTTTTGGTTTTGAACAGCTTACCATTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTcc                                                 <  1:1197703/149‑1 (MQ=255)
                                                                                                       gATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCACCGTACAGCTGAATGCGACCGCCAACGTCCTg                                               >  1:685424/1‑150 (MQ=255)
                                                                                                           gACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAgg                                           >  2:989878/1‑150 (MQ=255)
                                                                                                           gACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAgg                                           <  1:1285677/150‑1 (MQ=255)
                                                                                                            aCAGTTCTCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAggg                                          <  1:1232260/150‑1 (MQ=255)
                                                                                                            aCAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGTCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAggg                                          <  2:918049/150‑1 (MQ=255)
                                                                                                            aCAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGTCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAggg                                          <  2:922683/150‑1 (MQ=255)
                                                                                                             cAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGt                                         <  1:386247/150‑1 (MQ=255)
                                                                                                               gTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTaa                                       >  1:647544/1‑150 (MQ=255)
                                                                                                               gTTCGCCGGTTGTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTaa                                       <  2:574452/150‑1 (MQ=255)
                                                                                                                  ggcaggttttggttttGAACAGCTTACCATTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACTGGCAACGTACAGCTGAATGCTACCGCCAACGTCCTGCAGGGTAACGa                                    <  2:826410/146‑1 (MQ=255)
                                                                                                                  cGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCCTTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa                                    >  2:629026/1‑150 (MQ=255)
                                                                                                                  cGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAACCGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa                                    >  2:1183440/1‑150 (MQ=255)
                                                                                                                    ccGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACTTCCTGCAGGGTAACGaaa                                  <  2:1090022/150‑1 (MQ=255)
                                                                                                                     cGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGGCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAg                                 <  1:1183619/150‑1 (MQ=255)
                                                                                                                          ttggttttgAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCt                            <  2:1229692/150‑1 (MQ=255)
                                                                                                                           tggttttgAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCtt                           <  1:487573/150‑1 (MQ=255)
                                                                                                                            ggttttgAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCttt                          >  1:446436/1‑150 (MQ=255)
                                                                                                                             gttttgAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTa                         >  1:305189/1‑150 (MQ=255)
                                                                                                                              ttttgAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGCCGCTTTAc                        >  1:740343/1‑150 (MQ=255)
                                                                                                                                ttgAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTAccc                      >  2:1267675/1‑150 (MQ=255)
                                                                                                                                   aaCAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCata                   <  1:562468/150‑1 (MQ=255)
                                                                                                                                           aCCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCCACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATACTacgacg           >  1:988839/1‑150 (MQ=255)
                                                                                                                                            ccTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGCCCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACgg          >  2:656919/1‑150 (MQ=255)
                                                                                                                                                 cgcgCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAACCGCCTTCCGGGAGCTCGTCCCGGGCAACGTACAGCTGAAGGCGACCGCCAACGTCCTGCAGGGTAACTAACGCCGCTTTACCCTTAATACGACGGGtcat     >  1:662964/1‑150 (MQ=255)
                                                                                                                                                    gcCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTcatcat  >  1:1277134/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCAT  >  NC_000913/3034658‑3034955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: