BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	312,304	A→T	7.2%	I65L (ATA→TTA) ‡	ykgL →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	312,304	0	A	T	7.2%	242.8 / 4.2	97	I65L (ATA→TTA) ‡	ykgL	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base A (51/38); new base T (4/4); total (55/42)
Fisher's exact test for biased strand distribution p-value = 7.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

AGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCGCT  >  NC_000913/312155‑312453

aGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTta                                                                                                                                                       <  1:388843/150‑1 (MQ=255)

 gTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTtat                                                                                                                                                      >  1:1228978/1‑150 (MQ=255)

  tCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTtata                                                                                                                                                     <  2:1301513/150‑1 (MQ=255)

    tAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATaaa                                                                                                                                                   >  1:666873/1‑150 (MQ=255)

     aaaaGAGGGTTGATTCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGATTATTCTGAGAGAAACGTCATGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAATATTAATGTTGGTGTTATAAac                                                                                                                                                  <  1:213731/150‑1 (MQ=255)

         gagGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAacacac                                                                                                                                              <  1:579527/150‑1 (MQ=255)

           gggTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCa                                                                                                                                            >  2:385654/1‑150 (MQ=255)

           gggTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCa                                                                                                                                            <  2:946219/150‑1 (MQ=255)

            ggTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCaa                                                                                                                                           >  2:83538/1‑150 (MQ=255)

                gATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTGTTTATAACACCAACAtt                                                                                                                                       <  2:114111/150‑1 (MQ=255)

                 aTGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTGTTTATAACACCAACATTa                                                                                                                                      <  1:135031/150‑1 (MQ=255)

                   gCCAGCATACAGGGGCGAAGCTGGGCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTGTTTATAACACCAACATTAAt                                                                                                                                    <  2:780507/150‑1 (MQ=255)

                       gCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGa                                                                                                                                <  1:751674/150‑1 (MQ=255)

                         aTACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATc                                                                                                                              <  2:153977/150‑1 (MQ=255)

                          tACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCt                                                                                                                             >  2:1263649/1‑150 (MQ=255)

                            cAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATAAATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGAACTGa                                                                                                                           >  1:122971/1‑150 (MQ=255)

                              ggggCGAAGCTGGTCAACAAGTCAACATTAAGATAATGCAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAAACCACCAACATTAATCCGATCTGatt                                                                                                                         >  2:365457/1‑150 (MQ=255)

                               gggCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGatta                                                                                                                        <  2:614934/150‑1 (MQ=255)

                               gggCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGatta                                                                                                                        <  2:864865/150‑1 (MQ=255)

                                ggCGAAGCTGGTCAACAAGGCAACATTAAGATAATGGAATATTCGGAGAGACACGTCAGGCAGTTGGCCTCAAATTAACAGGAAGAATATAACCCCATGAAAATTAATGTTGGTGGTATAAACACACCAACATTAATCCGAGCTGattat                                                                                                                       <  1:184770/150‑1 (MQ=255)

                                  cGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGAttatta                                                                                                                     <  1:605096/150‑1 (MQ=255)

                                     aGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGTAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCACATGAAAATTAATGTTTGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATc                                                                                                                  <  2:685021/150‑1 (MQ=255)

                                     aGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAACATTAATGTTGGTGTTATTAACCCACCATCATGAGTCCGATGTGATGATTAATc                                                                                                                  >  2:1146953/1‑150 (MQ=255)

                                     aGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTTATTATTAAta                                                                                                                  >  2:637085/1‑149 (MQ=255)

                                     aGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATc                                                                                                                  <  1:133471/150‑1 (MQ=255)

                                      gCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCa                                                                                                                 >  2:403914/1‑150 (MQ=255)

                                         ggTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGAc                                                                                                              >  1:641055/1‑150 (MQ=255)

                                         ggTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGGTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATACGATCTGATTATTAATCataa                                                                                                              >  1:287877/1‑147 (MQ=255)

                                         ggTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGACAGGCAGTTGGCCTCAAATGAACAGGAAGAAAATAACCCCAGGAAAATTAATGTTGGTGTTAAAAACACACCAACATTAATACGATATGATTATTAATCAGaa                                                                                                              >  1:980098/1‑149 (MQ=255)

                                          gTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAAAAGGAAGAATATATGCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAAACCGATCTGATTATTAATAAGACa                                                                                                             >  2:592498/1‑150 (MQ=255)

                                            caacaaGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGTCATTTTGCCTCAAATGCTCAGGAAGAATATATCACCAGGAAAATTAATGTTTTTGTTATAAACACACCAACATTAATCCGATCTGATTAATAATCAGatcgg                                                                                                           <  2:196326/150‑5 (MQ=255)

                                            caacaaGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATAAGAaaaa                                                                                                           >  1:345767/1‑150 (MQ=255)

                                                  gTCAACATTAAGATAATGGAATATTCGGCGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCTCCAGGAAAATTAATGTTGGTGTTATAAAAACACCAACATTAATACGATCTGATTATTAATCAGACAAATATCGt                                                                                                     >  1:368472/1‑150 (MQ=255)

                                                  gTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGt                                                                                                     >  1:287099/1‑150 (MQ=255)

                                                    cAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTa                                                                                                   >  1:878178/1‑150 (MQ=255)

                                                     aaCATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTaa                                                                                                  <  2:723873/150‑1 (MQ=255)

                                                       cATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACGCCAACATTAATACGATCTGATTATTAATCAGACAAATATCGTTAATc                                                                                                >  2:1209979/1‑150 (MQ=255)

                                                         ttAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCg                                                                                              <  2:499457/150‑1 (MQ=255)

                                                         ttAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCg                                                                                              >  1:430288/1‑150 (MQ=255)

                                                            aGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCg                                                                                           >  1:468510/1‑150 (MQ=255)

                                                               tAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACCCCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAAt                                                                                        >  2:502666/1‑150 (MQ=255)

                                                                  tGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAAtttt                                                                                     >  2:351134/1‑150 (MQ=255)

                                                                   ggAATATTCGGAGAGAACCGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATGATTAATCAGACAAATATCGTTAATCCGGCGAATTTTg                                                                                    >  1:1293136/1‑150 (MQ=255)

                                                                   ggAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATTTATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTg                                                                                    >  2:435162/1‑150 (MQ=255)

                                                                      atatTCGGAGAGAACCGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAAt                                                                                 <  2:584857/150‑1 (MQ=255)

                                                                       tatTCGGAGAGAAAGGTCAGGCAGTTGGCCTGAAATGAACAGGAAGAATATATTCCCAGGAAATTTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGTCAAATATCGTTAATCCGTCGAATTTTGAATc                                                                                <  2:596834/150‑1 (MQ=255)

                                                                       tatTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATc                                                                                >  2:1281783/1‑150 (MQ=255)

                                                                           cGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACg                                                                            >  1:119504/1‑150 (MQ=255)

                                                                           cGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACg                                                                            <  2:266967/150‑1 (MQ=255)

                                                                            ggAGAGAAAAGTCACGCAGTTGGCCTAAAATGATCAAGAAGAATTTATAACAAGGAAAATTAATGTTGGTGTTTTAAAAACAAAAAAAATAATCAGATATGATTTATAATCATACAAAAAAATGTAAACCGTCATAGTTTGAATCAACGt                                                                           >  2:1187972/1‑150 (MQ=255)

                                                                                agaAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCACAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGttttt                                                                       <  1:83538/150‑1 (MQ=255)

                                                                                 gaAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGtttttt                                                                      >  2:423716/1‑150 (MQ=255)

                                                                                 gaAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGtttttt                                                                      >  1:529345/1‑150 (MQ=255)

                                                                                   aaCGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCt                                                                    >  1:215540/1‑150 (MQ=255)

                                                                                   aaCGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCt                                                                    <  1:1263649/150‑1 (MQ=255)

                                                                                     cGTCAGGCAGGTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTa                                                                  <  1:1092096/150‑1 (MQ=255)

                                                                                      gTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGATAATCCGTCGAATTTTGAATAAAATTTTTTTCTTAc                                                                 >  1:90879/1‑150 (MQ=255)

                                                                                           gCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACTAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGAccc                                                            <  2:125674/150‑1 (MQ=255)

                                                                                                tGGCCTCAAATGAAAATTAAGAATATTTCCCAAGGTAAATTAATTGTGGTGATATAAACACAGCAACATTAATCCGATCTGATTATTAATCAGACTAATATCTTTAATCCGGCTAATTTTGAATCAACGTTTTTTCTTACGACCCTGAAc                                                       <  1:190479/150‑1 (MQ=255)

                                                                                                  gCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACgg                                                     <  2:311024/150‑1 (MQ=255)

                                                                                                   ccTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGc                                                    >  2:396958/1‑150 (MQ=255)

                                                                                                   ccTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATAAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGc                                                    >  2:1064228/1‑150 (MQ=255)

                                                                                                    cTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGcc                                                   >  2:746511/1‑150 (MQ=255)

                                                                                                     tCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCTTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCt                                                  <  2:510691/150‑1 (MQ=255)

                                                                                                     tCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCt                                                  >  1:1292162/1‑150 (MQ=255)

                                                                                                     tCAAATGAACAGGAACAATATATCCACAGGAAAATTAAATTTGGTGTTTTAAACACACCAACATTAATCCGATCTGATTAATAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCt                                                  <  2:121050/150‑1 (MQ=255)

                                                                                                      cAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCtt                                                 >  2:1161702/1‑150 (MQ=255)

                                                                                                      cAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATACGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCtt                                                 <  2:1136635/150‑1 (MQ=255)

                                                                                                      aaaaTGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCtt                                                 <  1:704919/149‑1 (MQ=255)

                                                                                                       aaaTGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTa                                                <  2:716642/150‑1 (MQ=255)

                                                                                                        attGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACTACCCTGAACTGCCTTaa                                               >  2:567629/3‑150 (MQ=255)

                                                                                                          tGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTaaaa                                             >  2:303598/1‑150 (MQ=255)

                                                                                                            aaCAGGAAGAATATATCCACAGGAAAATTAATGTTGGTGTTATAAACACACAAAGATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTGTTTTCTTACGACCCTGAACGGCCTTAAAACg                                           <  1:646342/150‑1 (MQ=255)

                                                                                                              cAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCATTAAAACGTg                                         >  2:116429/1‑150 (MQ=255)

                                                                                                               aGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTgg                                        >  1:1056646/1‑150 (MQ=255)

                                                                                                                ggAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTTATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGa                                       >  2:446883/1‑150 (MQ=255)

                                                                                                                ggAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGa                                       <  1:403914/150‑1 (MQ=255)

                                                                                                                 gaagaaTATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGAAAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTAAGACACTGAACGGCATTAAAACGTGGAt                                      >  1:160951/1‑150 (MQ=255)

                                                                                                                  aagaaTATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGAtt                                     <  1:1161702/150‑1 (MQ=255)

                                                                                                                               cAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATc                        >  1:1058741/1‑150 (MQ=255)

                                                                                                                                 ggAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATcac                      <  1:1260661/150‑1 (MQ=255)

                                                                                                                                  gAAAATTAATGTTGGTGTTATAAACACACCATCATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATcaca                     <  1:351134/150‑1 (MQ=255)

                                                                                                                                  gAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATcaca                     <  2:929705/150‑1 (MQ=255)

                                                                                                                                   aaaaTTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACat                    >  1:50428/1‑150 (MQ=255)

                                                                                                                                    aaaTTAATGTTGGTGTTATAAACACACCAACCTTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGCCCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACata                   >  1:940817/1‑150 (MQ=255)

                                                                                                                                     gaTTAATGTTGGTGTGTTTATAACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACataa                  >  2:386214/2‑149 (MQ=39)

                                                                                                                                     aaTTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACatat                  <  1:746511/150‑1 (MQ=255)

                                                                                                                                        tAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAACACGGGGATTAGATTTACAAATCACATATAGc               <  1:562625/150‑1 (MQ=255)

                                                                                                                                        tAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGc               <  2:902847/150‑1 (MQ=255)

                                                                                                                                         aaTGTTGGTGTTATAAACACACCAACATTAATCCGATGTGATTATTAATCAGACAAATATCGTTAATCCGTCGTATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAATACGTGGATTAGATATACAAATCACATATAGcc              >  2:643720/1‑150 (MQ=255)

                                                                                                                                         aaTGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGcc              >  2:643718/1‑150 (MQ=255)

                                                                                                                                          aTGTTGGTGTGTTTATAACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCg             >  1:1024215/1‑150 (MQ=255)

                                                                                                                                           tgttgttgtTATAAACACAACAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGt            <  1:231280/150‑1 (MQ=255)

                                                                                                                                           tGTTGGTGTGTTTATAACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGt            >  1:1125898/1‑150 (MQ=255)

                                                                                                                                                 tgtTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTAAATCCGTCGAATATTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTcggttt      >  1:727917/1‑145 (MQ=255)

                                                                                                                                                  gtTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTc     >  2:1241616/1‑150 (MQ=255)

                                                                                                                                                  gtTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTc     >  1:544476/1‑150 (MQ=255)

                                                                                                                                                  gtTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTc     >  2:1250453/1‑150 (MQ=255)

                                                                                                                                                   ttATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCg    <  1:1027702/150‑1 (MQ=255)

                                                                                                                                                    tataAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCGc   >  1:451866/1‑150 (MQ=255)

AGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCGCT  >  NC_000913/312155‑312453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`