Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,042,315 T→A 9.1% intergenic (+22/+174) ygfZ → / ← yqfA iron‑sulfur cluster repair protein, plumbagin resistance/hemolysin III family HylIII inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,042,3150TA 9.1% 208.6 / 9.6 84intergenic (+22/+174)ygfZ/yqfAiron‑sulfur cluster repair protein, plumbagin resistance/hemolysin III family HylIII inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (37/39);  new base A (3/5);  total (40/44)
Fisher's exact test for biased strand distribution p-value = 7.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01

TGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGACGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCT  >  NC_000913/3042166‑3042463
                                                                                                                                                     |                                                                                                                                                    
tGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGTGTACGCGACGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCAt                                                                                                                                                      <  2:948068/150‑1 (MQ=255)
 ggAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGACGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCAtt                                                                                                                                                     >  1:468355/1‑150 (MQ=255)
 ggAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGACGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCAtt                                                                                                                                                     >  1:32945/1‑150 (MQ=255)
     gATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGACGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATc                                                                                                                                                 >  1:1159238/1‑150 (MQ=255)
         gTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAGCCGGATAGCATCTTCCGCGTACGAGACGATGCGAAAACATTGCAAATCGAGACGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGTCATTTATCGcaa                                                                                                                                             >  2:936152/1‑148 (MQ=255)
         gTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGACGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCt                                                                                                                                             >  2:1137095/1‑150 (MQ=255)
           cAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGACGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGa                                                                                                                                           <  1:994202/150‑1 (MQ=255)
           cAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGACGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGa                                                                                                                                           >  2:738287/1‑150 (MQ=255)
           cAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGACGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGa                                                                                                                                           <  1:637494/150‑1 (MQ=255)
             ggTCGTGGTACAGGTTGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGACGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATg                                                                                                                                         >  2:4410/1‑150 (MQ=255)
             ggTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGACGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCGATAAATGCCTGATg                                                                                                                                         <  1:930215/150‑1 (MQ=255)
             ggTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGACGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATg                                                                                                                                         >  1:716345/1‑150 (MQ=255)
             ggTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGACGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATg                                                                                                                                         >  1:548745/1‑150 (MQ=255)
             gctCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGACGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATg                                                                                                                                         <  2:88093/148‑1 (MQ=255)
              gtgggggTACAGGGCGGCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGACGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGc                                                                                                                                        <  2:923474/145‑1 (MQ=255)
              gTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGACGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCGATAAATGCCTGATGc                                                                                                                                        <  1:1305378/150‑1 (MQ=255)
              gTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGACGATGCGAATAAATTGCATATCGAGCCGCTGCCATATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGc                                                                                                                                        <  2:850025/150‑1 (MQ=255)
               tCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGACGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTTAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCg                                                                                                                                       >  2:697899/1‑150 (MQ=255)
                 gTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGACGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCGATAAATGCCTGATGCGat                                                                                                                                     <  1:537304/150‑2 (MQ=255)
                 gTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGACGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCGATAAATGCCTGATGCGat                                                                                                                                     <  1:1050483/150‑2 (MQ=255)
                 gTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGACGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCGATAAATGCCTGATGCGat                                                                                                                                     <  1:437464/150‑2 (MQ=255)
                  tGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGACGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACg                                                                                                                                    <  2:145467/150‑1 (MQ=255)
                         ggTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGACGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGgcgc                                                                                                                             >  2:156506/1‑150 (MQ=255)
                          gtcgtcATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGACGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGcgcg                                                                                                                            >  1:1254934/1‑150 (MQ=255)
                           tcgtcATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGACGATGCGAATACATTGTATTTATATAATGTTACGTATTCGATCGATTTGTAAATGTCTTTTTCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCgtgt                                                                                                                           <  2:782367/150‑1 (MQ=255)
                             gtcATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGACGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCt                                                                                                                         >  2:1113947/1‑150 (MQ=255)
                                  gAATAACGATATGGAACCGGATAGCATCATCCGCGTACGCGACGATGCGAATACAATGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCCCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCa                                                                                                                    <  2:71305/150‑1 (MQ=255)
                                          atatGGAACCGTATTGCATCTTCCGCGTACGCGACGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAATAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTggg                                                                                                            <  1:510118/150‑1 (MQ=255)
                                               taaCCGTATAGCTGCTTCCGCGTACGCGACGATGCTAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAAGCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTg                                                                                                       <  2:1211780/149‑1 (MQ=39)
                                               gAACCGGATAGCATCTTCCGCGTACGCGACGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTg                                                                                                       >  1:919186/1‑150 (MQ=255)
                                                  ccGGATAGCATCTTCCGCGTACGCGACGATGCGAATACATTTCATTTCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATGTATCGCCTGCTGCGCCGCTGGCGCGTCTTAGCTTGTCTGGGAttggctg                                                                                                    >  2:232682/1‑145 (MQ=255)
                                                   cgtaTAGCATCTTCCGCGTACGCGACGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCTTTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGc                                                                                                   <  1:879826/147‑1 (MQ=255)
                                                   cgcaTAGCAGATTCCGCGTACGCGACGATGCTAATACATTGAATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTTATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGc                                                                                                   <  1:518699/147‑1 (MQ=255)
                                                    ggATAGCATCTTCCGCGTACGCGACGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATgac                                                                                                  >  2:651704/1‑148 (MQ=255)
                                                    cgATACCATCTTCCGCGTACCCTACGACGCTAATACATTGCATATCGTCCCGCTGCCGTATTCGATCGAAGAGTAAAACTCTTTATCGCATCAGGCATTTAACGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGcc                                                                                                  <  2:329281/149‑1 (MQ=37)
                                                     tatatCATCTTCCGGGTCCGCGACTATGCTAATACATTGCTTATCGCGCCGGGGCCGAATTCGCTCGATGAGTAAATCTCTTTATCGCATCAGGCTTTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCt                                                                                                 <  2:119896/149‑1 (MQ=255)
                                                     gATAGCATCTTCCGCGTACGCGACGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCt                                                                                                 >  1:1133456/1‑150 (MQ=255)
                                                      aTAGCATCTTCCGCGTACGCGACGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCtt                                                                                                <  2:882557/150‑1 (MQ=255)
                                                       tAGCATCTTCCGCGTACGCGACGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCttt                                                                                               >  1:1107507/1‑150 (MQ=255)
                                                        aGCATCTTCCGCGTACGCGACGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCAAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTa                                                                                              >  2:941978/1‑150 (MQ=255)
                                                         gCATCTTCCGCGTACGCGACGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTAt                                                                                             >  2:54781/1‑150 (MQ=255)
                                                         gCATCTTCCGCGTACGCGACGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTAt                                                                                             >  1:703127/1‑150 (MQ=255)
                                                          cATCTTCCGCGTACGCGACGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATc                                                                                            <  2:813708/150‑1 (MQ=255)
                                                               tCCGCGTACGCGACGATGCGAATACAATGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCAATTATCGCCTGATGCGACGCTGGCGCGGCTTATCAGGCCTGGGATTTGATGCCTGTATcacatc                                                                                       >  2:630073/1‑148 (MQ=39)
                                                                ccGCGTACGCGACGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCacaaca                                                                                      <  2:898333/150‑1 (MQ=255)
                                                                       cgcgACGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGa                                                                               >  1:363006/1‑150 (MQ=255)
                                                                         cgaagaTGATAATACATTGCATATCGAGACGCTGCCTTATTCGCTCTAAGAGTAAATCTATTATTCGAAACAGGAATTTTTCGCCTGATGCGACGCTGTCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAgg                                                                             <  1:248897/150‑1 (MQ=255)
                                                                             gaTGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCg                                                                         <  1:156506/150‑1 (MQ=255)
                                                                                gCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAg                                                                      >  1:1116349/1‑150 (MQ=255)
                                                                                 cGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGa                                                                     <  2:74859/150‑1 (MQ=255)
                                                                                    aTACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCg                                                                  >  2:1037584/1‑150 (MQ=255)
                                                                                       cATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGAAGCCCCGGTGGCGCGTCTTATCATGTCTCGGATTAGCTGCCTTTAGCACAACAGCGACGAGGCCCGTAGATCAGAt                                                               >  1:357062/1‑150 (MQ=255)
                                                                                        aTTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAGTCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATa                                                              >  2:1229870/1‑150 (MQ=255)
                                                                                        aTTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATa                                                              >  2:895252/1‑150 (MQ=255)
                                                                                         atGCATATCGTGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTAATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATaa                                                             <  2:715201/149‑1 (MQ=255)
                                                                                           gCATTTCGAGCCGCTGCCGTATTCGCTCGAAGTGTAAATCTCTTTATCGCATCTGGCATTTATCGCCTGATGGGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAgg                                                           <  2:495773/150‑1 (MQ=255)
                                                                                           gCATCTCGAGCCGCTGCCGTATTCTCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAgg                                                           <  2:45644/150‑1 (MQ=255)
                                                                                             atatCGAGCCGCGGCCGTATTCGCTCGAAGCGTAAATCTCTTTATCGCATCAGGCATTAATCGCCTGATGCGACGCTGTCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCg                                                         <  1:235935/150‑1 (MQ=255)
                                                                                                         tGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTAACATGTCTGGGATTAGATGCCTTTATCACAACAGGGACGAGGCCCGAAGATCGGATAAGGAGTTAACGACGAAt                                             >  1:160447/1‑150 (MQ=255)
                                                                                                          gCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATc                                            >  1:779144/1‑150 (MQ=255)
                                                                                                           ccGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATcc                                           >  1:304539/1‑150 (MQ=255)
                                                                                                           ccGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATcc                                           >  1:494385/1‑150 (MQ=255)
                                                                                                            cGTATTCGCTCGAAGAGTAAATCTCTTTATCGCAGCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCg                                          <  1:746463/150‑1 (MQ=255)
                                                                                                               ttgcGCTCGAAGATTAAATCTATATATCCCATACGGCATTTAGCCCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCa                                       <  2:1156047/147‑1 (MQ=255)
                                                                                                               aTTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATTCGGCGTGAACGCCTTATCCgatc                                       <  2:32945/150‑4 (MQ=39)
                                                                                                               aTTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATTCGGCGTGAACGCCTTATCCgatc                                       <  1:77586/150‑4 (MQ=39)
                                                                                                               aTTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATGTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATTCGGCGTGAACGCCTTATCCgatc                                       <  1:935586/150‑4 (MQ=255)
                                                                                                                    cTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTtgt                                  >  2:748163/1‑150 (MQ=255)
                                                                                                                     tCGAAGATTAAATCTCTTTATCGCATCAGGCATTTTTCGCCTTATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTtgtg                                 <  2:1071087/150‑1 (MQ=255)
                                                                                                                     tCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTGGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTtgtg                                 >  2:658496/1‑150 (MQ=255)
                                                                                                                      cGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGc                                <  2:475267/150‑1 (MQ=255)
                                                                                                                      cGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCGTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGc                                >  2:322045/1‑150 (MQ=255)
                                                                                                                        aagataaaaTCTCTTTATCGAATCAGGAATTTATCGCCTTATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTc                              <  2:642581/144‑1 (MQ=255)
                                                                                                                                 tctcTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCATATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTg                     <  2:694281/150‑1 (MQ=255)
                                                                                                                                    ctTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATg                  >  2:592487/1‑150 (MQ=255)
                                                                                                                                           gCATCAGGCGATAAATGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCt           >  1:812033/1‑150 (MQ=38)
                                                                                                                                           gCATCAGGCGATAAATGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCt           >  2:1134156/1‑150 (MQ=38)
                                                                                                                                           gCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCt           <  2:816229/150‑1 (MQ=255)
                                                                                                                                            cATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTAACGCCGAATCCGCCAGTTGTGCTCCGATGCCTGATGCGCCGCTg          >  1:887689/1‑150 (MQ=255)
                                                                                                                                              tCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATCCCTGATTCGACGCTggg        >  1:821580/1‑149 (MQ=255)
                                                                                                                                               ccggCATATATCGCCTGAGGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGgcg       <  1:291178/148‑1 (MQ=255)
                                                                                                                                               cAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGgcg       <  2:919186/150‑1 (MQ=255)
                                                                                                                                               cAGGCATTACTCCCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGgcg       <  2:438041/150‑1 (MQ=255)
                                                                                                                                                 ggCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGcgcg     >  2:1123426/1‑150 (MQ=255)
                                                                                                                                                   cATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTc   >  2:150433/1‑150 (MQ=255)
                                                                                                                                                   cATTTATCGCCTGATGCGACGCTGGCGCGGCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTc   <  1:52995/150‑1 (MQ=255)
                                                                                                                                                    aTTTATCTCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCt  <  2:884333/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TGGAAGATGGTCAGGTCGTGGTACAGGTCGTCATGAATAACGATATGGAACCGGATAGCATCTTCCGCGTACGCGACGATGCGAATACATTGCATATCGAGCCGCTGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCT  >  NC_000913/3042166‑3042463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: