Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 312,306 A→T 7.1% I65I (ATA→ATT) ‡ ykgL → uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913312,3060AT 7.1% 267.6 / 7.4 95I65I (ATA→ATT) ‡ykgLuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base A (49/39);  new base T (4/3);  total (53/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01

TCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCGCT  >  NC_000913/312157‑312453
                                                                                                                                                     |                                                                                                                                                   
tCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTtata                                                                                                                                                     <  2:1301513/150‑1 (MQ=255)
  tAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATaaa                                                                                                                                                   >  2:953329/1‑150 (MQ=255)
  tAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATaaa                                                                                                                                                   >  1:666873/1‑150 (MQ=255)
   aaaaGAGGGTTGATTCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGATTATTCTGAGAGAAACGTCATGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAATATTAATGTTGGTGTTATAAac                                                                                                                                                  <  1:213731/150‑1 (MQ=255)
       gagGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAacacac                                                                                                                                              <  1:579527/150‑1 (MQ=255)
         gggTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCa                                                                                                                                            <  2:946219/150‑1 (MQ=255)
         gggTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCa                                                                                                                                            >  2:385654/1‑150 (MQ=255)
          ggTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCaa                                                                                                                                           >  2:83538/1‑150 (MQ=255)
              gATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTGTTTATAACACCAACAtt                                                                                                                                       <  2:114111/150‑1 (MQ=255)
               aTGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTGTTTATAACACCAACATTa                                                                                                                                      <  1:135031/150‑1 (MQ=255)
                 gCCAGCATACAGGGGCGAAGCTGGGCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTGTTTATAACACCAACATTAAt                                                                                                                                    <  2:780507/150‑1 (MQ=255)
                     gCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGa                                                                                                                                <  1:751674/150‑1 (MQ=255)
                       aTACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATc                                                                                                                              <  2:153977/150‑1 (MQ=255)
                        tACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCt                                                                                                                             >  2:1263649/1‑150 (MQ=255)
                          cAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATAAATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGAACTGa                                                                                                                           >  1:122971/1‑150 (MQ=255)
                            ggggCGAAGCTGGTCAACAAGTCAACATTAAGATAATGCAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAAACCACCAACATTAATCCGATCTGatt                                                                                                                         >  2:365457/1‑150 (MQ=255)
                             gggCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGatta                                                                                                                        <  2:864865/150‑1 (MQ=255)
                             gggCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGatta                                                                                                                        <  2:614934/150‑1 (MQ=255)
                              ggCGAAGCTGGTCAACAAGGCAACATTAAGATAATGGAATATTCGGAGAGACACGTCAGGCAGTTGGCCTCAAATTAACAGGAAGAATATAACCCCATGAAAATTAATGTTGGTGGTATAAACACACCAACATTAATCCGAGCTGattat                                                                                                                       <  1:184770/150‑1 (MQ=255)
                                cGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGAttatta                                                                                                                     <  1:605096/150‑1 (MQ=255)
                                   aGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGTAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCACATGAAAATTAATGTTTGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATc                                                                                                                  <  2:685021/150‑1 (MQ=255)
                                   aGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAACATTAATGTTGGTGTTATTAACCCACCATCATGAGTCCGATGTGATGATTAATc                                                                                                                  >  2:1146953/1‑150 (MQ=255)
                                   aGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTTATTATTAAta                                                                                                                  >  2:637085/1‑149 (MQ=255)
                                   aGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATc                                                                                                                  <  1:133471/150‑1 (MQ=255)
                                    gCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCa                                                                                                                 >  2:403914/1‑150 (MQ=255)
                                       ggTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGAc                                                                                                              >  1:641055/1‑150 (MQ=255)
                                       ggTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGGTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATACGATCTGATTATTAATCataa                                                                                                              >  1:287877/1‑147 (MQ=255)
                                       ggTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGACAGGCAGTTGGCCTCAAATGAACAGGAAGAAAATAACCCCAGGAAAATTAATGTTGGTGTTAAAAACACACCAACATTAATACGATATGATTATTAATCAGaa                                                                                                              >  1:980098/1‑149 (MQ=255)
                                        gTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAAAAGGAAGAATATATGCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAAACCGATCTGATTATTAATAAGACa                                                                                                             >  2:592498/1‑150 (MQ=255)
                                          caacaaGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGTCATTTTGCCTCAAATGCTCAGGAAGAATATATCACCAGGAAAATTAATGTTTTTGTTATAAACACACCAACATTAATCCGATCTGATTAATAATCAGatcgg                                                                                                           <  2:196326/150‑5 (MQ=255)
                                          caacaaGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATAAGAaaaa                                                                                                           >  1:345767/1‑150 (MQ=255)
                                                gTCAACATTAAGATAATGGAATATTCGGCGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCTCCAGGAAAATTAATGTTGGTGTTATAAAAACACCAACATTAATACGATCTGATTATTAATCAGACAAATATCGt                                                                                                     >  1:368472/1‑150 (MQ=255)
                                                gTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGt                                                                                                     >  1:287099/1‑150 (MQ=255)
                                                  cAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTa                                                                                                   >  1:878178/1‑150 (MQ=255)
                                                   aaCATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTaa                                                                                                  <  2:723873/150‑1 (MQ=255)
                                                     cATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACGCCAACATTAATACGATCTGATTATTAATCAGACAAATATCGTTAATc                                                                                                >  2:1209979/1‑150 (MQ=255)
                                                       ttAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCg                                                                                              <  2:499457/150‑1 (MQ=255)
                                                       ttAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCg                                                                                              >  1:430288/1‑150 (MQ=255)
                                                          aGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCg                                                                                           >  1:468510/1‑150 (MQ=255)
                                                             tAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACCCCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAAt                                                                                        >  2:502666/1‑150 (MQ=255)
                                                                tGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAAtttt                                                                                     >  2:351134/1‑150 (MQ=255)
                                                                 ggAATATTCGGAGAGAACCGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATGATTAATCAGACAAATATCGTTAATCCGGCGAATTTTg                                                                                    >  1:1293136/1‑150 (MQ=255)
                                                                 ggAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATTTATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTg                                                                                    >  2:435162/1‑150 (MQ=255)
                                                                    atatTCGGAGAGAACCGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAAt                                                                                 <  2:584857/150‑1 (MQ=255)
                                                                     tatTCGGAGAGAAAGGTCAGGCAGTTGGCCTGAAATGAACAGGAAGAATATATTCCCAGGAAATTTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGTCAAATATCGTTAATCCGTCGAATTTTGAATc                                                                                <  2:596834/150‑1 (MQ=255)
                                                                     tatTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATc                                                                                >  2:1281783/1‑150 (MQ=255)
                                                                         cGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACg                                                                            <  2:266967/150‑1 (MQ=255)
                                                                         cGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACg                                                                            >  1:119504/1‑150 (MQ=255)
                                                                          ggAGAGAAAAGTCACGCAGTTGGCCTAAAATGATCAAGAAGAATTTATAACAAGGAAAATTAATGTTGGTGTTTTAAAAACAAAAAAAATAATCAGATATGATTTATAATCATACAAAAAAATGTAAACCGTCATAGTTTGAATCAACGt                                                                           >  2:1187972/1‑150 (MQ=255)
                                                                              agaAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCACAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGttttt                                                                       <  1:83538/150‑1 (MQ=255)
                                                                               gaAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGtttttt                                                                      >  2:423716/1‑150 (MQ=255)
                                                                               gaAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGtttttt                                                                      >  1:529345/1‑150 (MQ=255)
                                                                                 aaCGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCt                                                                    >  1:215540/1‑150 (MQ=255)
                                                                                 aaCGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCt                                                                    <  1:1263649/150‑1 (MQ=255)
                                                                                   cGTCAGGCAGGTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTa                                                                  <  1:1092096/150‑1 (MQ=255)
                                                                                    gTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGATAATCCGTCGAATTTTGAATAAAATTTTTTTCTTAc                                                                 >  1:90879/1‑150 (MQ=255)
                                                                                         gCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACTAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGAccc                                                            <  2:125674/150‑1 (MQ=255)
                                                                                              tGGCCTCAAATGAAAATTAAGAATATTTCCCAAGGTAAATTAATTGTGGTGATATAAACACAGCAACATTAATCCGATCTGATTATTAATCAGACTAATATCTTTAATCCGGCTAATTTTGAATCAACGTTTTTTCTTACGACCCTGAAc                                                       <  1:190479/150‑1 (MQ=255)
                                                                                                gCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACgg                                                     <  2:311024/150‑1 (MQ=255)
                                                                                                 ccTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGc                                                    >  2:396958/1‑150 (MQ=255)
                                                                                                 ccTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATAAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGc                                                    >  2:1064228/1‑150 (MQ=255)
                                                                                                  cTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGcc                                                   >  2:746511/1‑150 (MQ=255)
                                                                                                   tCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCTTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCt                                                  <  2:510691/150‑1 (MQ=255)
                                                                                                   tCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCt                                                  >  1:1292162/1‑150 (MQ=255)
                                                                                                   tCAAATGAACAGGAACAATATATCCACAGGAAAATTAAATTTGGTGTTTTAAACACACCAACATTAATCCGATCTGATTAATAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCt                                                  <  2:121050/150‑1 (MQ=255)
                                                                                                    cAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCtt                                                 >  2:1161702/1‑150 (MQ=255)
                                                                                                    cAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATACGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCtt                                                 <  2:1136635/150‑1 (MQ=255)
                                                                                                    aaaaTGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCtt                                                 <  1:704919/149‑1 (MQ=255)
                                                                                                     aaaTGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTa                                                <  2:716642/150‑1 (MQ=255)
                                                                                                      attGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACTACCCTGAACTGCCTTaa                                               >  2:567629/3‑150 (MQ=255)
                                                                                                        tGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTaaaa                                             >  2:303598/1‑150 (MQ=255)
                                                                                                          aaCAGGAAGAATATATCCACAGGAAAATTAATGTTGGTGTTATAAACACACAAAGATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTGTTTTCTTACGACCCTGAACGGCCTTAAAACg                                           <  1:646342/150‑1 (MQ=255)
                                                                                                            cAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCATTAAAACGTg                                         >  2:116429/1‑150 (MQ=255)
                                                                                                             aGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTgg                                        >  1:1056646/1‑150 (MQ=255)
                                                                                                              ggAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTTATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGa                                       >  2:446883/1‑150 (MQ=255)
                                                                                                              ggAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGa                                       <  1:403914/150‑1 (MQ=255)
                                                                                                               gaagaaTATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGAAAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTAAGACACTGAACGGCATTAAAACGTGGAt                                      >  1:160951/1‑150 (MQ=255)
                                                                                                                aagaaTATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGAtt                                     <  1:1161702/150‑1 (MQ=255)
                                                                                                                             cAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATc                        >  1:1058741/1‑150 (MQ=255)
                                                                                                                               ggAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATcac                      <  1:1260661/150‑1 (MQ=255)
                                                                                                                                gAAAATTAATGTTGGTGTTATAAACACACCATCATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATcaca                     <  1:351134/150‑1 (MQ=255)
                                                                                                                                gAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATcaca                     <  2:929705/150‑1 (MQ=255)
                                                                                                                                 aaaaTTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACat                    >  1:50428/1‑150 (MQ=255)
                                                                                                                                  aaaTTAATGTTGGTGTTATAAACACACCAACCTTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGCCCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACata                   >  1:940817/1‑150 (MQ=255)
                                                                                                                                   gaTTAATGTTGGTGTGTTTATAACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACataa                  >  2:386214/2‑149 (MQ=39)
                                                                                                                                   aaTTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACatat                  <  1:746511/150‑1 (MQ=255)
                                                                                                                                      tAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAACACGGGGATTAGATTTACAAATCACATATAGc               <  1:562625/150‑1 (MQ=255)
                                                                                                                                      tAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGc               <  2:902847/150‑1 (MQ=255)
                                                                                                                                       aaTGTTGGTGTTATAAACACACCAACATTAATCCGATGTGATTATTAATCAGACAAATATCGTTAATCCGTCGTATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAATACGTGGATTAGATATACAAATCACATATAGcc              >  2:643720/1‑150 (MQ=255)
                                                                                                                                       aaTGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGcc              >  2:643718/1‑150 (MQ=255)
                                                                                                                                        aTGTTGGTGTGTTTATAACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCg             >  1:1024215/1‑150 (MQ=255)
                                                                                                                                         tgttgttgtTATAAACACAACAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGt            <  1:231280/150‑1 (MQ=255)
                                                                                                                                         tGTTGGTGTGTTTATAACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGt            >  1:1125898/1‑150 (MQ=255)
                                                                                                                                               tgtTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTAAATCCGTCGAATATTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTcggttt      >  1:727917/1‑145 (MQ=255)
                                                                                                                                                gtTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTc     >  2:1250453/1‑150 (MQ=255)
                                                                                                                                                gtTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTc     >  1:544476/1‑150 (MQ=255)
                                                                                                                                                gtTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTc     >  2:1241616/1‑150 (MQ=255)
                                                                                                                                                 ttATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCg    <  1:1027702/150‑1 (MQ=255)
                                                                                                                                                  tataAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCGc   >  1:451866/1‑150 (MQ=255)
                                                                                                                                                   ataAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCGCt  <  2:1000307/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCGCT  >  NC_000913/312157‑312453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑